1.Role and bioinformatics analysis of UCP2 in cervical cancer
Jing JIANG ; Yan CHEN ; Hong WU
The Journal of Practical Medicine 2017;33(1):91-94
Objective To investigate the effect of UCP2 on cell proliferation, apoptosis, metastasis in cervical squamous carcinoma. Methods Plasmid?mediated downregulation of UCP2 was obtained in cervical cancer cell lines. MTT, flow cytometry and transwell chamber assay were conducted to detect the ability of proliferation, apoptosis and metastasis. Characteristics of UCP2 protein was analyzed by bioinformatics methods. Results (1)UCP2 was verified to be downregulated by qRT?PCR and Western blotting assay.(2)MTT, apoptosis assay and transwell chamber assay showed that the proliferation of SiHa cell was significantly inhibited, and the apoptosis rate was significantly increased, and metastasis was markedly deduced (P < 0.05). (3) Bioinformatic analysis showed that UCP2 was located in mitochondria with several phosphorylation sites, and UCP2 interacted with other proteins to produce biological effects. Conclusion UCP2 may play an important role in the occurrence and development of cervical cancer, and it is expected to be a new target for the early diagnosis and treatment of cervical cancer.
2.Study of Serum Interleukin-18,Tumor Necrosis Factor-Alpha,Interferon-Gama Level and Their Relationships in Children with Viral Myocarditis
nan, YANG ; hong-yan, ZHANG ; jing, WANG
Journal of Applied Clinical Pediatrics 2006;0(13):-
Objective To study the relationship and changes of cytokine interleukine-18(IL-18),tumor necrosis factor alpha(TNF-?)and interferon-gama(IFN-?)in viral myocarditis(VM).Method Changes of serum cytokine IL-18,TNF-? and IFN-? of 72 acute VM patients and 25 healthy children were detected by enzyme-linked immunosorbent assay(ELISA).Results 1.P-values represented the obvious significance of difference as comparing the concentration of serum cytokine IL-18,TNF-? and IFN-? of VM children with that of the control group(Pa
4.Intra-hospital transport nursing of patients with severe inhalation pulmonary injury treated with extracorporeal membrane oxygenation
Jing ZHANG ; Hong YAN ; Haijing ZHANG ; Hanying LIN ; Runnu JING
Modern Clinical Nursing 2017;16(7):17-20
Objective To explore the nursing experience of the safe intra-hospital transport of patients with the severe aspiration of the inhalation injury. Method The nursing measures for the intra-hospital transport of 2 cases of smoke pot inhalation injury caused by extracorporeal membrane oxygenation treatment were taken, including disease risk assessment, preparation for transport, organization of a transport team, effective vital signs monitoring during transport, extracorporeal membrane oxygenation (ECMO) pipeline monitoring and nursing and observation and nursing of complications. Result No emergency was found during the transport of patients and both of them were safely transported. Conclusion Such nursing measures as pre-transport assessment and preparation and bettering predictive nursing for the patients with severe inhalation pulmonary injury treated with extracorporeal membrane oxygenation are key to the safety during intra-hospital transport.
5.Study of clinicopathologic features and p53 gene alterations in renal epithelioid angiomyolipoma.
Yan ZHU ; Juan-hong SHI ; Jing ZHAO ; Jing-jing XU ; Lin-hui WANG ; Yong-wei YU
Chinese Journal of Pathology 2012;41(8):558-559
Adenoma, Acidophil
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metabolism
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Adult
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Angiomyolipoma
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genetics
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metabolism
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pathology
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Carcinoma, Renal Cell
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metabolism
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Codon
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Diagnosis, Differential
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Exons
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Female
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Follow-Up Studies
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Gene Deletion
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Genes, p53
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Humans
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Kidney Neoplasms
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genetics
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metabolism
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pathology
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Male
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Melanoma-Specific Antigens
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metabolism
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Middle Aged
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Retrospective Studies
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Tumor Suppressor Protein p53
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genetics
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metabolism
7.Quality standard for Naolingsu Tablets
Jing HAN ; Lizhang ZHOU ; Hong HAN ; Yan LU ;
Chinese Traditional Patent Medicine 1992;0(07):-
Objective:To establish the quality standard for Naolingsu Tablets.Methods: Applying the technique of TLC, Radix Ginseng and Herba Epimedii were identified. The content of icariin was determined by HPLC. Results: Radix Ginseng and Herba Epimedii can be determined by TLC. Icariin showed a good linear relationship in the range of 0.011 ~ 0.424 ?g and the average recovery is 102.1% , RSD is 1.0% . Conclusion: The methods are simple, sensitive and accurate with good reproducibility. The methods may be used for quality control of Naolingsu Tablets.
8.The Development of Endogenous CO and its Mechanism in Asthma
Hong WANG ; Yan LONG ; Jing WANG ; Weili LUAN
Chinese Pediatric Emergency Medicine 2001;8(1):20-21
Objective To explore the development of endogenous CO and its mechanisms in asthma.Methods 50 guinea pigs were equally separated 5 groups.After the models of asthmatic guinea pigs were established in 4 groups,3 groups of them were treated with dexamethasone,the specific stimulator(hemin)and inhibitor(Sn-PP)of HO-1 respectively.The rest of groups were asthmatic one and normal control one(NCG).From all tested animals,the COHb in blood and lung HO-1 activity were examined,the lung tissues were pathologically observed and immunohistochemically stained.Results In asthmatic and hemin stimulated groups,the levels of COHb with enhanced expression of lung HO-1 and their activity were significantly increased compared with NCG(t=4.43~9.97,P<0.01).For example,the lung HO-1 activities in asthmatic group were (881±361)pmol/(mg*pro*h),COHb (4.94±2.15)% respectively.In dexamethason prevented and Sn-PP inhibited groups,however,all determined levels were markedly reduced compared with asthmatic group(P<0.01).Conclusion The increased HO-1 had resulted in the growth of endogenous CO in asthma.
9.Application of Circulation Water to Induce Hypothermia in the Operation of Treating Upper end of Shinbone Tumor
Yan CHEN ; Hong LI ; Yanling SHE ; Xiuyan JING ; Runa WANG
Chinese Journal of Practical Nursing 2001;17(2):21-22
It had some advantages when microwave induced hyperthermia were used to treat inferior femora and superior shinbone tumor.The device of circulation water,which could induce hypothermia during the treatment procedure needed to be used.Large amount of normal saline at the room temperature was perfused through this device,so the temperature of surrounding normal tissues and organs was reducd and the functions of nerve,vessel and knee were protected.It is safe and convenient measure that keep the operation go on smoothly.Summarized 54 cases of superior shinbone tumor that were used this device in the operation and experienced that it provided an effectiveness support for the operation.
10.Inhibition of adenovirus-mediated recombinant Tum5 gene overexpression on human umbilical vein endothelial cells in physiological status
Yurong, JIA ; Wei, YANG ; Hong, ZHANG ; Yan, ZHANG ; Jing, SUN
Chinese Journal of Experimental Ophthalmology 2017;35(8):677-682
Background Tumstatin is the most active endogenous angiogenesis inhibitor,which has a marked inhibitory effect on pathological neovascularization,and Tum5 is an angiogenesis inhibitors fragment of fulllength tumstatin.Objective This study was to investigate the effects of adenovirus-mediated overexpression of recombinant Tum5 gene on the proliferation,migration and tube formation of human umbilical vein endothelial cells (HUVECs) in physiological status.Methods The empty adenoviral vector expressing green fluorescent protein (rAd-GFP) and the viral vector expressing recombinant Tum5 gene were constructed.The HUVECs cultured in RPMI1640 medium were divided into normal control group,empty vector group (rAd-GFP group) and Tum5 gene infection group (rAd-GFP-Tum5 group).The rAd-GFP and rAd-GFP-Tum5 adenoviral particles at the density of 1 × 1010/ml were added into the medium to infect the cells for 48 hours.The proliferation of the cells was assayed at 24,48 and 72 hours by cell counting kit-8 (CCK-8) to evaluate the proliferative rate;the migration number of the cells was detected at 48 hours after infection by Transwell chamber;the tube formation number of the cells were detected by Matrigel method.The concentration of vascular endothelial growth factor (VEGF) in cell supernatants was assayed by ELISA at 24,48,and 72 hours following adenoviral infection.Results The cultured cells showed green fluorescence in the rAd-GFP group and rAd-GFP-Tum5 group under the inverted fluorescence microscope,and the infection efficiency of rAd-GFP and rAd-GFP-Tum5 was 55.13% and 50.31%,respectively.No significant difference was found in cell proliferative rate among normal control group,rAd-GFP group and rAd-GFP-Tum5 group both at 24 and 48 hours after infection (both at P>0.05),and the cell proliferative rate was significantly lower in the rAd-GFP-Tum5 group than that in the normal control group and rAd-GFP group at 72 hours after infection (both at P<0.01).The migration number of the cells at 48 hours after infection was 2 260.25-±930.44,2 370.00±441.06 and 723.75± 363.80 in the normal control group,rAd-GFP group and rAd-GFP-Tum5 group,showing a significant difference among the groups (F =8.524,P =0.008),and the migrated cells were evidently decreased in the rAd-GFP-Tum5 group compared with the rAd-GFP group and the normal control group (both at P< 0.01).The tube number at 48 hours after infection was 95.67±5.86,88.00±4.58 and 20.67±3.51 in the normal control group,rAd-GFP group and rAdGFP-Tum5 group,showing a significant difference among the groups (F=226.498,P<0.01),and the tube number in the rAd-GFP-Tum5 group was significantly reduced in comparison with the normal control group and rAd-GFP group (both at P< 0.01).The considerably differences in VEGF concentration in the cell supernatants were found in different groups and various time points (Fgroup =73.260,P<0.01;Ftime =73.477,P<0.01),and VEGF concentration in the cell supernatants was significantly decreased in the rAd-GFP-Tum5 group compared with the rAd-GFP group at both 48 hours and 72 hours (both at P<0.01).Conclusions The overexpression of the recombinant Tum5 can inhibit the proliferation,migration and tube formation of the HUVECs in physiological status,which may be associated with Tum-5-mediated down-regulation of VEGF protein in the cell supernatant.