Bispheno|A(BPA)is an endocrine disruptor with estrgenic properties,which is a widely used industrial platicizer.When exposure to BPA for long-term,it would impact on multiple systems and organs including reproductive.developmental,neurological,endocrine function and so on,particularly in obesity.ExpoSUre to BPA during pregnancy and postnate,it can increase the sensitivity to obesity in offsprings.BPA may be contributed to obesity by enhancing triglyceride accumulation and accelerating adipocytes differentiation,affecting endocrine function of adipocytes and blood glucose and insulin metabolism.However,the dosage effect of BPA is not a simple linear rehionship.The impact of environment-related dosage on the human is worthy ot research in future.

Objective:To establish an HPLC method for the determination of two components in aspirin and phenobarbital pow-ders.Methods:AThermoBDSC18column(250mm×4.6mm,5μm)wasusedwithacetonitrile-methanol-0.01mol·L-1KH2PO4so-lution -triethylamine(10∶35∶55∶0.1)(adjusting pH to 3.5 with phosphoric acid )as the mobile phase. The flow rate was 1.0 ml· min-1 and the detection wavelength was 242nm. The column temperature was ambient and the injection volume was 20 μl. Results:There was a good linear relationship when the content of aspirin and phenobarbital was within the range of 0. 402 0 ~1. 608 0 mg· ml-1(r=0.999 7) and 0.061 2 ~0.244 8 mg·ml-1(r =0.999 6), respectively. The average recovery was 99.21%(RSD =0. 49%,n=9) and 99. 63% (RSD=0. 39%,n=9), respectively. Conclusion:The method is simple and accurate with good repro-ducibility, and can be used to control the quality of the preparation.

To establish an HPLC method for the determination of content and content uniformity of minoxidil tablets. Methods:An Xbridge C18 column (250 mm ×4.6 mm, 5 μm)was used with methanol-0.03 mol·L-1 KH2PO4 solution -triethyl-amine(60∶40∶0. 1)(adjusting pH to 3. 5 with phosphoric acid ) as the mobile phase. The flow rate was 1. 0 ml·min-1 and the detec-tion wavelength was 287 nm. The column temperature was 30℃ and the injection volume was 10 μl. Results:There was a good linear relationship within the range of 8. 056-32. 224 μg·ml-1(r=0. 999 9). The average recovery was 99. 68%(RSD=0. 36%,n=9). The average content of 3 batches of samples was 100. 2%,100. 5% and 99. 8% and the content uniformity was 5. 5,6. 2 and 4. 9, re-spectively. Conclusion:The method is simple and accurate with good reproducibility, which can be used in the quality control of mi-noxidil tablets.

Real-time three-dimensional echocardiography(RT-3DE) has been validated for its accurate diagnoses and quantitave mearsurement of mitral valve disease,which could act as a guide in selecting the proper therapeutic strategy for each individual in clinical setting.In this article,the recent research achievements on RT-3DE in mitral valve disease were summarized,and the application of RT-3DE in surgical approach to mitral valve disease was discussed as well.

In order to achieve the demand of clinical diagnosis,treatment and research based on TCM clinical literature,we conducted the questionnaire to doctors of Chinese medicine on their demands for medical information in the process of clinical diagnosis and treatment,hoping to make certain the direction and service methods of TCM clinical research database from the actual demands of doctors.

OBJECTIVE:To evaluate the rationality of drug use in cancer patients in Tiantan Hospice Hospital.METHODS:By a retrospective study,the drug use in cancer patients in Tiantan Hospice Hospital who died during Jan.2008 and Sep.2008 were analyzed statistically.RESULTS & CONCLUSION:The drug use in cancer patients in Tiantan Hospice Hospital was rational on the whole.Step medication,oral medication,on time medication and individual medication were realized in Beijing Tiantan Hospice hospital and treatment on cancer patients in this hospital was in line with the Guideline for Cancer Pain Management Based on the Three-step Analgesic Ladder recommended by WHO.

Objective:To develop a method to simultaneously determine the content of imperatorin, isoimperatorin and magnolin in Quchuan capsules by HPLC. Methods:A Shiseido CAPCELL PAK C18 column(150 mm × 4. 6 mm,5 μm) was used. The mobile phase consisted of acetonitrile(A)-water(B) with gradient elution at a flow rate of 1. 0 ml·min-1 and the column temperature was at 30℃. The detection wavelength was set at 278 nm from 0 to 25 min and 248 nm from 25 to 70 min. The injection volume was 10 μl. Results:The linear range of imperatorin,isoimperatorin and magnolin was 6. 126-55. 134 μg·ml-1(r=0. 999 7), 9. 862-88. 758 μg ·ml-1(r =0.999 8) and 4.830-43.470 μg·ml-1(r =0.999 8), respectively. The average recovery was 101.5% (RSD =1. 4%), 99. 6%(RSD=1. 2%) and 100. 2% (RSD=1. 7%), respectively. Conclusion: The method is convenient, sensitive and accurate, which can be used for the quality control of Quchuan capsules.

Up to 90 % of multiple myeloma patients can develop to osteolytic lesions. Although the conventional X radiography can only demonstrate the lytic bone disease when 30 % of the trabecular bone loss, it still remains the gold standard of the staging procedure of newly diagnosed myeloma patients. CT scans are more sensitive than X radiography for detecting small lytic lesions. MRI or CT is the choice of diagnosis to assess suspected cord compression. Technicium-99m bone scan in patients with myeloma usually underestimate the extent of bone damage. Treatments of myeloma bone disease include bisphosphonate therapy, novel targeted agents, surgical intervention and radiation therapy.

Objective To observe the effects of chemotherapy on the peripheral blood lymphocyte sub-cluster in patients with ovarian cancer .Methods Sixty-two patients with ovarian cancer who accepted chemo-therapy from Jan 2007 to Mar 2013 in our hospital were collected .Sixty healthy women in our hospital were used as control.The expressions of CD3,CD4,CD8 and CD25 in peripheral blood lymphocyte of ovarian cancer patients before,one week and one month after chemotherapy and healthy women were detected and analyzed .Results The ratio of CD3 +and CD4 +T cells and CD4 +/CD8 +in patients with ovarian cancer were significantly lower than those in healthy .Whereas the ratio of CD4 +CD25 +T cells in patients with ovarian cancer was significantly higher than that in healthy women .No significant differences were observed in ovarian cancer patients with differ-ent clinical stage .There were no significant changes that observed one week after chemotherapy in patients with o -varian cancer .These indexes improved notably one month after chemotherapy .These indexes in effective group one month after chemotherapy were significantly better than those in inefficient group .Conclusion The ratio of pe-ripheral blood lymphocyte sub -cluster in patients with ovarian cancer was imbalance .Dynamic monitoring the changes could guide the therapy and judge the curative effect and prognosis .

Collaborative inquiry applied in teaching of literature retrieval course is beneficial for the unity and cooperation between teachers and students,and their independent study ability. Described in this paper is the collabo-rative inquiry in teaching of information retrieval course with its target, methods and program carefully designed for medical postgraduates according to the students-oriented construction theory, which can promote the growth up of teachers and students, and learn the frontier and innovative theories, and practical knowledge.