1.Analysis of measles antibody level and susceptibility of enterprise employees in Jinshan District,Shanghai in 2019
ZHOU Jie ; MA Xiao⁃min ; WANG Fang ; LI Shu⁃hua
Chinese Journal of Biologicals 2023;36(1):65-69
Abstract:Objective To analyse the level of measles antibody and serological susceptibility of the enterprise employees in Jin⁃
shan District,Shanghai in 2019,to provide references for measles prevention and control of enterprise employees. Methods
A total of 1 796 employees were selected from 6 enterprises in Jinshan District by using a stratified random sampling method,of
which serum samples from intravenous blood were collected and determined for IgG antibody level of measles by ELISA. Geo⁃
metric mean concentration(GMC)of measles IgG antibody,antibody positive rate and antibody protection rate were calculated
and the susceptibility factors of measles in enterprise employees were analyzed by using the unconditional multivariate Logistic
regression model. Results The GMC of measles antibody in 1 796 monitoring objects was 639. 71 mIU/mL,the positive rate
was 87. 58% and the protection rate was 29. 06%. Multivariate Logistic regression analysis showed that the odds ratio(OR)of
measles susceptibility was 1. 55(95% CI:1. 14 ~ 2. 12)in native subjects compared with non⁃resident subjects,while 2. 35
(95% CI:1. 35 ~ 4. 08)in subjects aged 40 ~ 49 years compared with those aged ≥ 50 years. Conclusion In 2019,the
measles antibody level of enterprise employees in Jinshan District of Shanghai was low,and people aged 40 ~ 49,especially the
nativeadults,wereathighriskofsusceptibilitytomeasles. Supplementaryimmunizationwithmeaslesvaccineshouldbecarried
out for high⁃risk enterprise employees to reduce the risk of measles infection and control the epidemic situation of measles.
2.Establishment of a simple and convenient animal model of cardiac arrest for studying cerebral resuscitaion
Yu-Jie MA ; Xing-Yi YANG ; Zhao-Fen LIN ; Lei ZHANG ; Shu-Ming PAN ;
Chinese Journal of Emergency Medicine 2006;0(09):-
Objective To establish an simple and convenient animal model of cardiac arrest for studying cerebral resuscitation.Method Clean male Sprague Dawley rats were randomly divided into sham group and experimental group.Cardiac arrest was induced by asphyxiation and ice-cold 0.5 mol KCl with blood flow cut off for 5 minutes.Animals were resuscitated with external cardiopulmonary resuscitation (CPR),mechanical ventilation,and epinephrine injection.Blood pressure,heart rate,successful ratio of resuscitation after 72 hours, time of cardiac arrest (T_(CA)) and return of spontaneous circulation (T_(ROSC)) were recorded.Neural deficit scores (NDS) and levels of maleic dialdehyde (MDA) in plasma were evaluated at 3,6,12,24,48,72 hours after ROSC.The damage score of cortex was measured by transmission electron microscope examination at 3 hours and 72 hours after ROSC.Results All the rats in experimental group had cardiac arrest rapidly.T_(CA) and T_(ROSC) were (137.3?10.2) seconds and (64.4?9.3) seconds,respectively,while the successful rate of resuscitation was 82.5%.The lowest NDS was at 3 hours after ROSC,while the NDS increased gradually.After CPR,the level of MDA in plasma increased significantly,slightly declined at 72 hours after ROSC,but still significantly higher than before the model.Electron microscope examination of cortex showed neuron slightly,organelle and astrocyte,but became better after 72 hours post ROSC.Conclusions The model of cardiac arrest was easy to establish,and the data provided was accurate,which is useful to study the mechanism of cerebral resuscitation.
3.Discrimination of patients with Xiao-Chaihu Tang syndrome using 1H NMR metabonomics and partial least square analysis.
Jie XING ; Shu-chun YUAN ; Hui-min SUN ; Ma-li FAN ; Zhen-yu LI ; Xue-mei QIN
Acta Pharmaceutica Sinica 2015;50(8):1032-1037
1H NMR metabonomics approach was used to reveal the chemical difference of urine between patients with Xiao-Chaihu Tang syndrome (XCHTS) and healthy participants (HP). The partial least square method was used to establish a model to distinguish the patients with Xiao-Chaihu-Tang syndrome from the healthy controls. Thirty-four endogenous metabolites were identified in the 1H NMR spectrum, and orthogonal partial least squares discriminant analysis showed the urine of patients with Xiao-Chaihu Tang syndrome and healthy participants could be separated clearly. It is indicated that the metabolic profiling of patients with Xiao-Chaihu Tang syndrome was changed obviously. Fifteen metabolites were found by S-pot of OPLS-DA and VIP value. The contents of leucine, formic acid, glycine, hippuric acid and uracil increased in the urine of patients, while threonine, 2-hydroxyisobutyrate, acetamide, 2-oxoglutarate, citric acid, dimethylamine, malonic acid, betaine, trimethylamine oxide, phenylacetyl glycine, and uridine decreased. These metabolites involve the intestinal microbial balance, energy metabolism and amino acid metabolism pathways, which is related with the major symptom of Xiao-Chaihu Tang syndrome. The patients with Xiao-Chaihu Tang syndrome could be identified and predicted correctly using the established partial least squares model. This study could be served as the basis for the accurate diagnostic and reasonable administration of Xiao-Chaihu-Tang syndrome.
Humans
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Least-Squares Analysis
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Medicine, Chinese Traditional
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Metabolome
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Metabolomics
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Proton Magnetic Resonance Spectroscopy
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Syndrome
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Urinalysis
4.Effects of volatile oil of Rhizoma zingiberis and other two kinds of volatile oil on percutaneous penetration of bullatine A via hairless mouse skin in vitro.
Yi-Cen BAI ; Yan-Jie LI ; Yun-Shu MA
China Journal of Chinese Materia Medica 2008;33(5):513-516
OBJECTIVETo investigate the effects of volatile oils of Rhizoma Acori Tatarinowii (RAT), Semen Myristicae (SM) and Pericarpium Citri Reticulatae (PCR) on percutaneous penetration of bullatine A via hairless mouse skin in vitro.
METHODBy an improved Franz diffusion, the effects of three kinds of volatile oils on the percutaneous penetration of bullatine A were observed and compared with Azone, and the cumulative amount of bullatine A was determined by HPLC.
RESULTThe penetration enhancement ratios of bullatine A with 7% volatile oil RAT and SM, 5% volatile oil of PCR and 3% Azone were 6.52, 6.74, 2.18, 6.03, respectively.
CONCLUSIONThe volatile oil of RAT, SM and PCR enhance permeation of bullatine A, effectively.
Administration, Cutaneous ; Animals ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; administration & dosage ; pharmacokinetics ; pharmacology ; Male ; Mice ; Mice, Nude ; Oils, Volatile ; isolation & purification ; pharmacology ; Plants, Medicinal ; chemistry ; Skin ; drug effects ; metabolism ; Skin Absorption ; drug effects
5.Effect of SonoVue on left and right ventricle in pigs
Shufu CHANG ; Juying QIAN ; Jianying MA ; Zhangwei CHEN ; Lili DONG ; Leilei CHENG ; Jie CUI ; Xianhong SHU ; Junbo GE
Chinese Journal of Ultrasonography 2010;19(5):431-434
Objective To assess the effect of ultrasound contrast agent SonoVue on the dimensions and systolic function of left and right ventricle in pigs. Methods Sixteen pigs were randomly assigned to two groups. Intravenous injection of 1 ml of SonoVue were given in study group, and repeated 20 min later. The control group was given the same doses of saline. Before and after the administration of contrast agent, the end-diastolic dimension (LVEDD, RVEDD). end-systolic dimension ( LVESD, RVESD) and fractional shortening(LVFS,RVFS) of left and right ventricle were measured. The time to reach the extreme value of these parameters and the time to return to the baseline were recorded. Results There was no significant difference regarding the parameters at baseline between the two groups. After injection of SonoVue,RVEDD significantly increased from (25. 88 ± 1. 38) mm at baseline to its maximum of (33. 26 ± 0. 99)mm( P < 0. 05). Accordingly,RVFS significantly increased from (26. 90 ± 1. 92) % to (33. 92 ± 2. 53) % ( P <0. 05). Meanwhile,LVEDD remarkably decreased from (38.10 ± 1. 39)mm at baseline to its minimum of (26.25 ± 0. 65)mm( P <0. 05) and LVFS remarkably decreased from (36. 24 ± 1. 93) % to (29.13 ± 3.00) % ( P < 0. 05). There was no change in the control group after administration of the saline. When SonoVue was given repeatedly, the maximum RVEDD and RVFS was (29. 98 ± 1. 23) mm and (31. 09 + 1.90) % , respectively, which had less increase compared to the first time. Minimum LVEDD and LVFS was (31. 91 ± 1, 64)mm and (32. 17 ± 2. 31)%,respectively,with less decrease compared with which at first injection. It took (10. 15±0. 59) min for the right and left ventricle to reach the extreme value and (9.00± 0. 56) min to return to the baseline at the first injection. The time used for the right and left ventricle to reach its peak change and back to baseline after second injection of SonoVue were shorter [(8.73± 0.55) min and (6.89± 0.43) min, respectively,both P <0.05]. Conclusions Administration of SonoVue was associated with acute, transient dilation of right ventricle and compression of left ventricle. The influence of SonoVue on the right and left ventricle became less at it second injection.
6.Effect of Compound Zhajin Granule on Toll-like Receptor 4 Signaling Pathway in Nonalcoholic Steatohepatitis Mice.
Jing LIU ; Zhen-jie ZHUANG ; Jing TIAN ; Zhao-yi LI ; Yan LUO ; Yin-lan LIU ; Jian-yu CHEN ; Xiao-jie MA ; Shu-fei ZANG ; Jun-ping SHI
Chinese Journal of Integrated Traditional and Western Medicine 2016;36(4):460-465
OBJECTIVETo observe the effect of Compound Zhajin Granule (CZG) on Toll-like re-ceptor 4 (TLR4) signaling pathway in high-fructose corn syrup induced NASH mice.
METHODSThirty 6-week-old male C3H mice were divided into the high fat and high fructose (HFHFr) group (n = 20) and the control group (n = 10) according to body weight. Mice in the HFHFr group ate high fat diet and drank 20% fructose water, while those in the control group ate common diet and drank common water. After 8 weeks mice in the HFHFr group were divided into two group according to body weight, the HFHFr group and the CZG group, 10 in each group. Mice in the CZG group were fed with high fat forage and 20% fructose water, and administered with 50 mL/kg 12. 8% CZG (prepared by hawthorn, Radix Curcumae, Alisma Orientale, Fritillaria Thunbergii, Silybum Marianum, peach seed in the ratio of 3:1.5:1.5:2:1.5:2:1) by gastrogavage. Mice in the HFHFr group were fed in the same way and daily administered with equal volume of distilled water by gastrogavage. Sixteen weeks later all mice were sacrificed. Body weight, liver wet weight, liver function, and lipid metabolism were detected. Pathological changes of liver tissues were assessed by HE staining, oil red O staining, and Masson staining. Expressions of TLR4, myeloid differentiation factor 88 (MyD88), tumor necrosis factor-alpha (TNF-α) were detected using immunohistochemical staining and real-time fluorescent quantitative PCR.
RESULTSBody weight, alanine aminotransferase (ALT), aspartate aminotransferase (AST) were obviously lower in the CZG group than in the HFHFr group (P < 0.05); oil red O stained area and density were decreased more in the CZG group than in the control group. HE staining showed ballooning inflammation was reduced more in the CZG group than in the HFHFr group. Masson staining was negative. Positive rates of TLR4 and MyD88 and mRNA expressions were significantly lower in the CZG group than in the HFHFr group (all P < 0.05).
CONCLUSIONCZG could significantly inhibit TLR4 signaling pathway of liver in NASH mice.
Alanine Transaminase ; metabolism ; Animals ; Aspartate Aminotransferases ; metabolism ; Diet, High-Fat ; Drugs, Chinese Herbal ; pharmacology ; Fructose ; administration & dosage ; adverse effects ; Inflammation ; Lipid Metabolism ; Male ; Mice ; Mice, Inbred C3H ; Myeloid Differentiation Factor 88 ; metabolism ; Non-alcoholic Fatty Liver Disease ; drug therapy ; Signal Transduction ; drug effects ; Toll-Like Receptor 4 ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism
7.Comparison of data population-based and from hospital-based injuries.
Ling-ni ZHOU ; Jie-min MA ; Zhong-jie LI ; Shu-yang CHEN ; Gong-huan YANG
Chinese Journal of Epidemiology 2004;25(11):967-969
OBJECTIVETo compare data from an epidemiological survey on injuries with a survey conducted in hospitals on injuries in the same areas and to find out the differences and shortcomings of hospital data in describing the feature of injuries in an area.
METHODSComparing the causes and age distributions of injuries from the two surveys.
RESULTSThe first 4 leading causes of injuries from the population-based survey were mechanical injuries, falls, burns/scalds and traffic accidents while the first 4 leading causes of hospital-based survey were traffic accidents, assault, mechanical injuries and burns/scalds. The differences of the age distributions of these leading causes between the two surveys were significant except mechanical injuries.
CONCLUSIONDifferences were noticed between population-based survey and hospital-based survey. It should be cautions when using hospital data to describe the features of injuries in a certain area.
Accidental Falls ; statistics & numerical data ; Accidents, Traffic ; statistics & numerical data ; Adolescent ; Adult ; Burns ; epidemiology ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Hospitalization ; Humans ; Incidence ; Male ; Middle Aged ; Rural Health ; Wounds and Injuries ; epidemiology
8.Impact of mobile phone radiation on the quality and DNA methylation of human sperm in vitro.
Dong WANG ; Bo LI ; Yuan LIU ; Ye-fei MA ; Shu-qiang CHEN ; Hui-jun SUN ; Jie DONG ; Xu-hui MA ; Jing ZHOU ; Xiao-hong WANG
National Journal of Andrology 2015;21(6):515-520
OBJECTIVETo investigate the influences of mobile phone radiation on the quality and DNA methylation of human sperm in vitro.
METHODSAccording to the fifth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen, we randomly selected 97 male volunteers with normal semen parameters and divided each semen sample from the subjects into two equal parts, one exposed to mobile phone radiation at 1950 M Hz, SAR3. 0 W/kg for 3 hours while the other left untreated as the control. We obtained routine semen parameters as well as the acrosomal reaction ability, apoptosis and DNA methylation of sperm, and compared them between the two groups.
RESULTSCompared with the control, the radiation group showed significantly decreased progressive sperm motility ([36.64 ± 16.93] vs [27.56 ± 16.92]%, P < 0.01) and sperm viability ([63.72 ± 16.35] vs [54.31 ± 17.35]%, P < 0.01) and increased sperm head defects ([69.92 ± 4.46] vs [71.17 ± 4.89]%, P < 0.05), but no significant differences in sperm acrosomal reaction ([66.20 ± 6.75] vs [64.50 ± 3.47]%, P > 0.05). The early apoptosis rate of sperm cells was remarkably higher in the radiation group ([6.89 ± 9.84]%) than in the control ([4.44 ± 5.89]%) (P < 0.05). However, no statistically significant differences were found between the control and radiation groups in the DNA methylation patterns of the paternal imprinting gene H19 ICR ([0.60 ± 0.02] vs [1.40 ± 0.03]%, P > 0.05) or the maternal imprinting gene KvDMR1 ([0.00 ± 0.00] vs [1.80 ± 0.031%, P > 0.05).
CONCLUSIONMobile phone radiation reduces the progressive motility and viability of human sperm and increases sperm head defects and early apoptosis of sperm cells.
Cell Phone ; DNA Methylation ; radiation effects ; Humans ; In Vitro Techniques ; Male ; Semen ; radiation effects ; Semen Analysis ; Sperm Head ; radiation effects ; Sperm Motility ; radiation effects ; Spermatozoa ; cytology ; radiation effects
9.Determination of synephrine and N-methyltyramine in Pericarpium Citri Reticulatae viride by HPLC.
Xian-duan LI ; Zhi-jing MA ; Sheng LIN ; Xue-zhu GU ; Shu-jie MAO
China Journal of Chinese Materia Medica 2004;29(6):537-539
OBJECTIVETo establish a quantitative method for determination of synephrine and N-methyltyramine in Citri Reticulatae.
METHODSamples were extracted with 30% methanol. ODS column was used with methanol-water-sodium dodecyl sulfate (55:45:0.1) as mobile phase. Detection wavelength was 285 nm.
RESULTSynephrine and N-methyltyramine in sample solution were well separated. Linearity of synephrine was good (r = 0.9999) in range of 0.35-11.24 microg. The average recovery was 97.1%, and RSD of repeatability was 1.9%.
CONCLUSIONThis method can be used for quality control of Citri Reticulatae.
Chromatography, High Pressure Liquid ; Citrus ; chemistry ; Fruit ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control ; Seasons ; Synephrine ; analysis ; Tyramine ; analogs & derivatives ; analysis
10.Thymosin beta10 expression and actin filament organization in tumor cell lines with different metastatic potential.
Cong-rong LIU ; Chun-shu MA ; Jun-yu NING ; Jiang-feng YOU ; Song-lin LIAO ; Jie ZHENG
Chinese Journal of Pathology 2004;33(1):67-71
OBJECTIVETo investigate the expression of thymosin beta10 (Tbeta10) and related changes of actin filament organization in human tumor cell lines with different metastatic potential.
METHODSFour groups of nine human tumor cell lines with different metastatic potential were analyzed for the expression of Tbeta10 mRNA detected by northern-blot and its peptide by immunohistochemical staining. The filamentous actin (F-actin) was stained with TRITC-phalloidin to detect changes in actin organization.
RESULTSIn comparison with the non and/or weakly metastatic counterparts, Tbeta10 was upregulated in highly metastatic human lung cancer, malignant melanoma and breast cancer cell lines. TRITC-phalloidin staining revealed less actin bundles and a fuzzy network of shorter filaments in the highly metastatic tumor cells, while in the non and/or weakly metastatic cancer cell lines, there were thick and orderly arranged actin filaments.
CONCLUSIONSTbeta10 levels correlate positively with the metastatic phenotype in human tumors currently examined. The increased metastatic potential of tumor cells is accompanied by the loss of F-actin and poorly organized actin skeleton. There is a consistent correlation between the elevated Tbeta10 expression and the disrupted actin skeleton.
Actins ; analysis ; Blotting, Northern ; Cell Line, Tumor ; Humans ; Immunohistochemistry ; Neoplasm Metastasis ; Thymosin ; analysis