Objective To observe the effect of Auto-CPAP in the treatment of patients with obstructive sleep apnea-hypopnea syndrome,to sum up experience through the treatment.Methods We cured the patients with obstructive sleep apnea-hypopnea syndrome who had been diagnosed by polysomnogram by Auto-CPAP,then observed the improvement in their subjective feelings and objective target aim such as blood pressure and SaO2.Results After treatment by Auto-CPAP.we found their symptom had been improved significantly,for example,the time of sleep apnea decreased or disappeared,the sleep structure had been improved evidently.Conclusion As intelligent CPAP,Auto-CPAP can provide the lowest effective therapy pressure from beginning to end though the inductorium in the face piece,and increase the ease and toleration of the patients,so the curative effects are remarkable.

Short stature is diagnosed according to the person below the normal height of mean -2 standard de-viation or below the third percentile,respectively,of the same race,age,and sex.Diagnosis needs comprehensive assess-ment,including detailed medical history collection,detailed physical examination and appropriate laboratory tests.There are limitations of these factors in diagnosing the reasons of short stature,but the rapid progress on genomics and techno-logy enriched the diagnostic methods.Ongoing research efforts to identify genes influencing growth will provide a better understanding of mechanisms underlying abnormal growth and will eventually lead to novel management approaches.

The brief discussion is introduced in the paper on the academic thought of professor YAN Jie, the contemporary famous TCM doctor, on functional dyspepsia treated with acupuncture and moxibustion. Treatment based on "the three-regional acupoint selection" is applied to professor YAN's treatment for functional dyspepsia, in which, acupuncture is on Sibai (ST 2), Liangmen (ST 21) and Zusanli (ST 36), and the supplementary points are added accordingly. The academic thought is described as the combination of acupuncture and moxibustion based on strengthening healthy qi, supplemented by soothing the liver and psychological counseling. Also, an example is provided.
Acupuncture ; education ; history ; Acupuncture Points ; Acupuncture Therapy ; history ; Adult ; Dyspepsia ; history ; therapy ; Female ; History, 20th Century ; Humans ; Moxibustion

Eosinophils ; immunology ; Food Hypersensitivity ; etiology ; Gastritis ; etiology ; Humans ; Infant ; Male ; Milk Hypersensitivity ; complications ; immunology ; Milk Proteins ; immunology

BACKGROUND:Stem cells under appropriate conditions can be induced to differentiate into neuron-like cells. Chinese herbs can function in cellproliferation and differentiation.
OBJECTIVE:To investigate the interventional effects of traditional Chinese medicine on stem celltransplantation in the treatment of cerebrovascular diseases.
METHODS:Experimental studies concerning traditional Chinese medicine intervention for stem celltransplantation were retrieved and analyzed. Under specific conditions, stem cells can proliferate in vitro in a suspended or monolayer manner, and can be potential y induced to differentiate into neurons, oligodendrocytes and astrocytes, thereby providing basis for stem celltransplantation in the treatment of cerebrovascular diseases. We observed expressions of nestin, glial fibril ary acidic protein, and microtubule-associated protein expression for analysis of stem cellproliferation and differentiation.
RESULTS AND CONCLUSION:Baicalin or Astragalus saponin Ⅳ can stimulate stem cells to differentiate into nerve cells in vitro. Naomaitong or danshen combined with bone marrow mesenchymal stem celltransplantation can promote neurological recovery from cerebrovascular diseases in rats. Buyang Huanwu Decoction can significantly promote the proliferation of neural stem cells in the subventricular zone and neurological recovery after cerebral ischemia. Chinese herbs, such as Zhichan Decoction and Panax notoginseng, can promote survival of transplanted cells and prevent most cellapoptosis in the stem celltherapy for treatment of Parkinson’s disease. Therapy of benefiting qi and elevating yang or ginsenosides Rg1 combined with stem celltherapy can improve memory ability of dementia rats. Traditional Chinese medicine exerts a catalytic role in the stem celltherapy for cerebrovascular diseases.

AIM: To investigate whether potassium cyanate can inactivate glyceraldehydes 3-phosphate dehydrogenase (GAPDH) and thioltransferase (TTase) in bovine lens.METHODS: Fresh intact bovine lenses were incubated with 100mmol/L potassium cyanate (KCNO) for 7 and 12 days respectively. Then all lens were incubated in 50mmol/L DMEM solution. The proteins in the watersoluble fractions from the normal control and the cyanate-modified lens were extracted. The activity of GAPDH and TTase in the water-soluble fraction after incubation at 37℃ was measured by spectrophotometer.RESULTS: GAPDH activity was significantly lower in the cyanate-modified lens proteins than that of the normal control (P<0.01), and considerably diminished in protein incubated with 100mmol/L potassium cyanate for 12 days. There were statistically significant differences in the activity of TTase between the normal control lenses and the carbamylated lenses incubated for 7 days (P<0.05) and 12 days (P<0.01). However. there was no statistical difference between the samples incubated with 100mmol/L KCNO for 7 and 12 days (P=0.19296).CONCLUSION: This study provides evidence to show carbamylation is able to inactivate GAPDH and TTase in bovine lenses. This may have implications for the susceptibility of lenticular GAPDH and TTase to carbamylation, and also for the research on pathogenesis of cataract.

Objective To explore variance and resource of intracellular free Ca2+ and extracellular arachidonic acid (AA) in different types of passage cells during the invasion of T. gondii. Methods The variance and resource of extracellular AA and intraceUular free Caz+ of Vero and J774A. 1 cells during the invasion of T. gondii were detected by multi-purpose scintillation counter and laser scanning confocal microscope. Data were analyzed using Chi-square test and t test. Results The intracellular free Ca2+ levels in J774A. 1 and Vero cells were both increased after T. gondii infection. The maximal changes of fluorescence intensity were (1 219.7±58.4)% (P＜0.01) and (356.3±23.6)% (P＜0.05), respectively.The increase of intracellular Ca2+ level in Vero cell was mostly from the release of intracellular Ca2+ store.And the Ca2+ increase in J774A. 1 cell was from both the release of intracellular Ca2+ store and extracellular Ca2+ influx. Extracellular AA levels were significantly increased in both Vero and J774A. 1 cells after T.gondii infection 5.02 and 8. 44 times respectively (t= 3. 124, t = 3. 852, P＜0.01). The AA elevations could be significantly inhibited by phospholipase A2 (PLA2) inhibitor pretreating T. gondii. Conclusions The phospholipase C of phagocytic host cell and PLA2 of T. gondii are activated by T. gondii infection,which results in the increase of intracellular free Ca2+ and extraeellular AA level. Combined actions of Ca2+and AA play a major role in the invasion of T. gondii to host cell. While only PLA2 of T. gondii may be activated in nonphagoeytic host cell.

Objective To investigate the learning and memory functions,expression changes of disintegrin and metalloprotease 10 (ADAM10) mRNA in hippocampus in the aged rats with chronic cerebral hypoperfusion as well as the effect of atorvastatin on them.Methods A total of 72 rats were randomly divided into sham operation,cerebral hypoperfusion and atorvastatin treatment groups.A permanent bilateral common carotid artery occlusion (2VO)model was induced.Atorvastatin 10 mg/(kg · d) was administered orally after procedure in the atorvastatin treatment group.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of ADAM10 mRNA in bilateral hippoocampus at 1,2,4,and 16 weeks after modeling,Results Two weeks after modeling,the learning and memory functions were decreased significantly in the cerebral hypoperfusion group compared to the sham operation group (P ＜ 0.05).At 4 and 16 weeks after modeling,they were further decreased (P ＜0.01); there were no significant differences in the learning and memory functions at 1,2,and 3 weeks after modeling between the atorvastatin treatment group and the cerebral hypoperfusion group,however,they were improved significantly at 16 weeks compared to the cerebral hypoperfusion group (P＜0.01).The expression of ADAM10 mRNA in hippocampus at different time points after modeling in the cerebral hypoperfusion group was down-regulated by 22％,43％,35％,and 50％,respectively compared to the sham operation group (all P ＜0.05).The expression of ADAM 10 mRNA in hippocampus at 2 weeks in the atorvastatin treatment group was higher than 22％ in the cerebral hypoperfusion group (P＜0.05).There were not significant differences at other time points.Conelusions Chronic cerebral hypoperfusion results in the down-regulation of the expression of ADAM10 mRNA in hippocampus in the aged rats,and atorvastatin may inhibit down-regulation of the expression of ADAM10 mRNA at early stage.

Sedation and analgesia for ventilated patients is an important treatment in intensive care unit (ICU). Patients receiving mechanical ventilation therapy comfortably and safely can improve patient-ventilator synchrony, reduce ventilation-related lung injury (VILI), improve compliance, decrease oxygen consumption and stress, prevent accidents, and reduce the incidence of complications and mortality in critical patients. Although sedation mayprotect lung function, it also has a greater impact on respiratory function. Therefore, based on domestic and overseas related researches about sedation therapy in ventilated patients, we expounded on the effects of sedation therapy, different sedatives and different sedative methods on respiratory function of ventilated patients. The effects of sedation improving patient-ventilator synchrony or protecting pulmonary function as well as problems about inhibition of spontaneous respiration or delayed extubation were discussed to provide references for sedation treatment in patients with mechanical ventilation.

[Summary] Pectus excavatum ( PE ) is a posterior intrusion of the chest wall into the thoracic cavity . Although the physiological and psychological consequences vary , for a large number of patients the lesions are troublesome enough that they need corrective surgery .The minimally invasive repair of pectus excavatum ( Nuss procedure ) has been performed safely , effectively , and extensively .The current situations and progresses associated with the Nuss procedure were reviewed .