Short stature is diagnosed according to the person below the normal height of mean -2 standard de-viation or below the third percentile,respectively,of the same race,age,and sex.Diagnosis needs comprehensive assess-ment,including detailed medical history collection,detailed physical examination and appropriate laboratory tests.There are limitations of these factors in diagnosing the reasons of short stature,but the rapid progress on genomics and techno-logy enriched the diagnostic methods.Ongoing research efforts to identify genes influencing growth will provide a better understanding of mechanisms underlying abnormal growth and will eventually lead to novel management approaches.

Objective To observe the effect of Auto-CPAP in the treatment of patients with obstructive sleep apnea-hypopnea syndrome,to sum up experience through the treatment.Methods We cured the patients with obstructive sleep apnea-hypopnea syndrome who had been diagnosed by polysomnogram by Auto-CPAP,then observed the improvement in their subjective feelings and objective target aim such as blood pressure and SaO2.Results After treatment by Auto-CPAP.we found their symptom had been improved significantly,for example,the time of sleep apnea decreased or disappeared,the sleep structure had been improved evidently.Conclusion As intelligent CPAP,Auto-CPAP can provide the lowest effective therapy pressure from beginning to end though the inductorium in the face piece,and increase the ease and toleration of the patients,so the curative effects are remarkable.

Objective To investigate the learning and memory functions,expression changes of disintegrin and metalloprotease 10 (ADAM10) mRNA in hippocampus in the aged rats with chronic cerebral hypoperfusion as well as the effect of atorvastatin on them.Methods A total of 72 rats were randomly divided into sham operation,cerebral hypoperfusion and atorvastatin treatment groups.A permanent bilateral common carotid artery occlusion (2VO)model was induced.Atorvastatin 10 mg/(kg · d) was administered orally after procedure in the atorvastatin treatment group.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of ADAM10 mRNA in bilateral hippoocampus at 1,2,4,and 16 weeks after modeling,Results Two weeks after modeling,the learning and memory functions were decreased significantly in the cerebral hypoperfusion group compared to the sham operation group (P ＜ 0.05).At 4 and 16 weeks after modeling,they were further decreased (P ＜0.01); there were no significant differences in the learning and memory functions at 1,2,and 3 weeks after modeling between the atorvastatin treatment group and the cerebral hypoperfusion group,however,they were improved significantly at 16 weeks compared to the cerebral hypoperfusion group (P＜0.01).The expression of ADAM10 mRNA in hippocampus at different time points after modeling in the cerebral hypoperfusion group was down-regulated by 22％,43％,35％,and 50％,respectively compared to the sham operation group (all P ＜0.05).The expression of ADAM 10 mRNA in hippocampus at 2 weeks in the atorvastatin treatment group was higher than 22％ in the cerebral hypoperfusion group (P＜0.05).There were not significant differences at other time points.Conelusions Chronic cerebral hypoperfusion results in the down-regulation of the expression of ADAM10 mRNA in hippocampus in the aged rats,and atorvastatin may inhibit down-regulation of the expression of ADAM10 mRNA at early stage.

Objective:Matching pursuit algorithm(MAP),for its good parametric characterization, is applied in Magnetoencephalography(MEG) to study time-frequency distribution. Methods:This paper proposes to apply a high-resolution time-frequency analysis algorithm, the matching pursuit (MP), to extract detailed time-frequency components of SEF signals. Results: Experimental results on cortical SEF signals of several normal subjects show that a series of stable SEF time components can be identified using the MP decomposition algorithm. Conclusion:This study shows that there is a set of stable and minute time-frequency componentsin SEF signals, which are revealed by the MP decomposition. These stable SEF components have specific localizations in the time domain and may provide a reliable index for clinical research of brain function and brain disease pathogenesis.

Objective To determine whether triggering receptor expressed on myeloid cells-1 (sTREM-1) and urinary neutrophil gelatinase-associated lipocalin (NGAL) were early biomarkers of acute kidney injury (AKI) secondary to sepsis.Methods A total of 141 eligible patients were enrolled in this prospective study.Blood and urine samples were collected at different time points as soon as sepsis was diagnosed.The concentrations of serum creatinine (Scr),urine sTREM-1 and NGAL were measured.According to AKI criteria,patients were divided into the AKI group and non-AKI group.Dynamic changes of levels of Scr,urine sTREM-1 and NGAL were observed in two groups.The receiver operating characteristic curves were used to evaluate the early diagnostic value of urine sTREM-1 and NGAL.Results Among 141 septic patients,44 (31.2％) cases had concomitant AKI.Twenty four hours after sepsis diagnosed,the level of Scr rose to 1.91 times of the baseline [(140.5±13.6) vs (82.6± 15.3) μmol/L,P ＜ 0.05],which met the diagnostic criteria of AKI.In the AKI group,urinary concentrations of sTREM-1 and NGAL at 8 h after the diagnosis of sepsis began to rise significantly from baseline [(100.5±17.4) vs (38.9± 14.7) ng/L; (144.6±51.9) vs (56.2±43.8) μg/L,both P ＜ 0.05].And at the following time points,urinary concentrations of sTREM-1 and NGAL were significantly higher than the baseline levels and that of the non-AKI group (all P ＜ 0.05).At 8 h time point,thearea under the curve of urine sTREM-1 was 0.877 (95％CI 0.756-0.914),the sensitivity was 89.1％ and specificity was 82.0％ with a cutoff value of 70 ng/L.At 8 h time point,the area under the curve of urine NGAL was 0.862 (95％ CI 0.703-0.958),the sensitivity was 87.4％ and specificity was 85.5％ with a cutoff value of 90 μg/L.Conclusions Urinary concentrations of sTREM-1 and NGAL at 8 h time point after the diagnosis of sepsis have predictive value for AKI and their diagnostic time is much earlier than that of Scr.Therefore,urinary sTREM-1 and NGAL can be used as early biomarkers of septic AKI.

Objective To evaluate the association between serum ferritin and the risk of cardiovascular disease among the Chinese population.Methods Based on the data from China Health and Nutrition Survey 2009, we used the Framingham risk score to calculate the 10-year risk of cardiovascular disease and analyzed the association between serum ferritin and the risk of cardiovascular disease in different gender by logistic regression analysis.Results Among 6 551 respondents, 3 035 (46.3%) were males and 3 516 ( 53.7%) were females whose mean age were ( 49.3 ±13.8 ) and ( 49.2 ±13.7 ) years, respectively.The median level of serum ferritin was 75.08 ( 36.71 -138.75 )μg/L.According to the 10-year risk of cardiovascular disease calculated by the Framingham risk score , the low risk group, middle risk group and high risk group were accounted for 79.5%( n=5 207 ) , 15.7% ( n=1 028 ) and 4.8%( n=316), respectively.The median of serum ferritin (121.61 vs.46.14, P<0.01) and the Framingham risk score (8.6 ±6.0 vs.7.3 ±1.6, P<0.01) of males were higher than those of females.The percentages of the middle risk group ( 30.6% vs.2.8%) and high risk group ( 9.8% vs.0.5%) of 10-year risk of cardiovascular disease in males were higher than those in females ( P <0.01 ).The 10-year risk of cardiovascular disease ≥10%increased with the increasing of serum ferritin in females ( trend P<0.01 ) but not in males.Conclusion Serum ferritin levels are positively correlated with the risk of cardiovascular disease in female Chinese.

AIM: To investigate whether potassium cyanate can inactivate glyceraldehydes 3-phosphate dehydrogenase (GAPDH) and thioltransferase (TTase) in bovine lens.METHODS: Fresh intact bovine lenses were incubated with 100mmol/L potassium cyanate (KCNO) for 7 and 12 days respectively. Then all lens were incubated in 50mmol/L DMEM solution. The proteins in the watersoluble fractions from the normal control and the cyanate-modified lens were extracted. The activity of GAPDH and TTase in the water-soluble fraction after incubation at 37℃ was measured by spectrophotometer.RESULTS: GAPDH activity was significantly lower in the cyanate-modified lens proteins than that of the normal control (P<0.01), and considerably diminished in protein incubated with 100mmol/L potassium cyanate for 12 days. There were statistically significant differences in the activity of TTase between the normal control lenses and the carbamylated lenses incubated for 7 days (P<0.05) and 12 days (P<0.01). However. there was no statistical difference between the samples incubated with 100mmol/L KCNO for 7 and 12 days (P=0.19296).CONCLUSION: This study provides evidence to show carbamylation is able to inactivate GAPDH and TTase in bovine lenses. This may have implications for the susceptibility of lenticular GAPDH and TTase to carbamylation, and also for the research on pathogenesis of cataract.

The brief discussion is introduced in the paper on the academic thought of professor YAN Jie, the contemporary famous TCM doctor, on functional dyspepsia treated with acupuncture and moxibustion. Treatment based on "the three-regional acupoint selection" is applied to professor YAN's treatment for functional dyspepsia, in which, acupuncture is on Sibai (ST 2), Liangmen (ST 21) and Zusanli (ST 36), and the supplementary points are added accordingly. The academic thought is described as the combination of acupuncture and moxibustion based on strengthening healthy qi, supplemented by soothing the liver and psychological counseling. Also, an example is provided.
Acupuncture ; education ; history ; Acupuncture Points ; Acupuncture Therapy ; history ; Adult ; Dyspepsia ; history ; therapy ; Female ; History, 20th Century ; Humans ; Moxibustion

Objective To explore variance and resource of intracellular free Ca2+ and extracellular arachidonic acid (AA) in different types of passage cells during the invasion of T. gondii. Methods The variance and resource of extracellular AA and intraceUular free Caz+ of Vero and J774A. 1 cells during the invasion of T. gondii were detected by multi-purpose scintillation counter and laser scanning confocal microscope. Data were analyzed using Chi-square test and t test. Results The intracellular free Ca2+ levels in J774A. 1 and Vero cells were both increased after T. gondii infection. The maximal changes of fluorescence intensity were (1 219.7±58.4)% (P＜0.01) and (356.3±23.6)% (P＜0.05), respectively.The increase of intracellular Ca2+ level in Vero cell was mostly from the release of intracellular Ca2+ store.And the Ca2+ increase in J774A. 1 cell was from both the release of intracellular Ca2+ store and extracellular Ca2+ influx. Extracellular AA levels were significantly increased in both Vero and J774A. 1 cells after T.gondii infection 5.02 and 8. 44 times respectively (t= 3. 124, t = 3. 852, P＜0.01). The AA elevations could be significantly inhibited by phospholipase A2 (PLA2) inhibitor pretreating T. gondii. Conclusions The phospholipase C of phagocytic host cell and PLA2 of T. gondii are activated by T. gondii infection,which results in the increase of intracellular free Ca2+ and extraeellular AA level. Combined actions of Ca2+and AA play a major role in the invasion of T. gondii to host cell. While only PLA2 of T. gondii may be activated in nonphagoeytic host cell.