3.Dispute and consensus about surgical approaches and lymph nodes excisional area of esophageal carcinoma.
Chinese Journal of Gastrointestinal Surgery 2011;14(9):667-670
Surgical approaches for esophageal carcinoma are many and varied. Minimally invasive techniques are increasingly used widely in esophagectomy as they not only ensure patients' therapeutic effects, but also reduce trauma and accelerate recovery. The lymph nodes excisional area of esophageal carcinoma is still in controversy. It is necessary to carry out further researches on selective lymph nodes excision, which can decrease complication rate and improve patients' survival in the meantime.
Esophageal Neoplasms
;
surgery
;
Esophagectomy
;
methods
;
Humans
;
Lymph Node Excision
;
methods
;
Minimally Invasive Surgical Procedures
4.The Clinico-pathological Features of Suspected Malignant Nodules Associated with Hashimoto's Thyroiditis-Diagnosis
Chen HUA ; Yongqing FU ; Jian ZHOU
Journal of Zhejiang Chinese Medical University 2013;(12):1382-1385,1386
[Objective]To analyze the clinical and biological characteristics of suspected malignant thyroid nodules associated with Hashimoto thyroiditis .To summarize different characteristics of benign and malignant nodules,diagnosis and treatment of suspected malignant nodules associated with Hashimoto's thy-roiditis. [Methods]Surgical y and pathological y confirmed, 58 cases of suspected malignant thyroid nodules associated with Hashimoto's thyroiditis were retro-spectively analyzed with clinical and pathological data. The differences of gender, age, number of nodules, calcification, and thyroid hormone and thyroid an-tibodies levels between malignant and benign nodules were compared from January 2009 to December 2011 .[Results] The average age of malignant thyroid nodules coexisting with HT group was significantly lower than that of benign nodules coexisting with HT group( P<0.05). The proportion of nodular calcifi-cation in malignant thyroid nodules coexisting with HT group was significantly higher than that in benign nodules coexisting with HT group( P<0.05). Pre-operative TSH and anti-TG levels in malignant thyroid nodules coexisting with HT group were significantly higher than that in benign nodules coexisting with HT group. Preoperative FT3, FT4, anti-TPO levels, sex ratio and number of lesions between the two groups were not statistical y significant. [Conclu-sions] The key point of preoperative diagnosis:patients of malignant thyroid nodules coexisting with HT are associated with youth, calcified nodules, elevated serum TSH and anti-TG levels.
5.Dynamic expression of transforming growth factor-beta1 mRNA and protein and its inhibitory effect on lung development in premature rat with chronic lung disease.
Chinese Journal of Pediatrics 2005;43(6):463-465
Animals
;
Chronic Disease
;
Disease Models, Animal
;
Female
;
Gene Expression Regulation, Developmental
;
Hyperoxia
;
Immunohistochemistry
;
Lung
;
growth & development
;
metabolism
;
Lung Diseases
;
metabolism
;
Oxygen
;
Pregnancy
;
RNA, Messenger
;
metabolism
;
Rats
;
Rats, Sprague-Dawley
;
Reverse Transcriptase Polymerase Chain Reaction
;
Transforming Growth Factor beta1
;
genetics
;
metabolism
7.Expression and Effect of Cyclin Dependent Kinase-4 and p21 in Premature Rats with Hyperoxia-Induced Chronic Lung Disease
Journal of Applied Clinical Pediatrics 2004;0(11):-
Objective To investigate the dynamic change of expression of cyclin dependent kinase-4(CDK4)and cyclin dependent kinase inhibitor(p21)in premature rats with hyperoxia-induced chronic lung disease(CLD)and its role.Methods Eighty premature rats were randomly and equally divided into model group(hyperoxia group)and control group(room air group).CLD was induced by hypemxia exposure.The expression of CDK4 and p21 were observed with immunohistochemical method,and the levels of type I collagen were detected by enzyme-linked immunosorbent assay on days 1,3,7,14 and 21.Results Compared with control group,in model group,the expression of CDK4 protein and level of type I collagen statistically increased,but expression of p21 protein decreased significantly on days 14 and 21.The expression of CDK4 was positive correlated with the degree of fibrosis,and expression of p21 was negative correlated with the degree of fibrosis in model group.Conclusion The expression of CDK4 increases and expression of p21 decreases in premature rats exposed to hyperoxia,which may play an important role in the lung fibrosis.
8.Several Issues Need to be Resolved in Implementing Health Management
Jing-Na WU ; Jian--Hua LU ; Fu--Ying WANG
Chinese Journal of Prevention and Control of Chronic Diseases 2006;0(06):-
Health management plays an important role in the control of medical costs and in maintaining people' s health.Since the beginning of health management performance in our country,the major problems were:inadequacy of govement's support;imperfect laws;irregular marketing operation;lack of feasible health management model;controversy over long-term economic benefits;lack of awareness on health in common people and so on.To solve these problems,it is necessary to strengthen government support,and enhance people's health awareness,and explore the health management model consistent with the actual situation in China.
9.Construction and application of a mouse HPRT targeting vector with Cre recombinase recognition site lox66
Jingmin ZHENG ; Jian LI ; Hua YANG ; Jiliang FU ;
Academic Journal of Second Military Medical University 1985;0(06):-
Objective: To study the construction and application of a vector with Cre recombinase recognition site lox66 for mouse HPRT gene targeting in embryonic stem(ES) cells. Methods: Using the HPRT genomic DNA fragment and synthesized oligonucleotides, pSP HPRT lox66 Neo was designed and constructed as a replacement vector by common molecular cloning techniques. After the linearized pSP HPRT lox66 Neo DNA was electroporated into ES cells, and transfected cells were cultured in G418/6 TG drug selection medium. The recombination efficiency of this vector was tested. Results: The main components of pSP HPRT lox66 Neo were a positive selection gene Neo, lox66, long and short homologous fragments of mouse HPRT gene and plasmid backbone. Twenty ES cell clones with HPRT gene inactivated were obtained. Conclusion: An effective replacement vector with Cre recombinase recognition site lox66 is constructed and applied to HPRT gene targeting in ES cells.