1.Changes of Serum Ferritin and Blood Lead Levels in Children with Attention Deficit Hyperactivity Disorder
Journal of Applied Clinical Pediatrics 2004;0(07):-
0.05).Conclusion Low iron stores contribute to ADHD.SF level must be evaluated and retrieved in subjects with ADHD,particularly in the subgroup of ADHD-I.
2.Clinical study on relationship between protein tyrosine kinase JAK2 V617F mutation and high altitude polycythemia.
Lin-hua JI ; Zhan-quan LI ; Sen CUI
Chinese Journal of Hematology 2012;33(4):319-320
Adult
;
Aged
;
Altitude
;
Humans
;
Janus Kinase 2
;
genetics
;
Male
;
Middle Aged
;
Point Mutation
;
Polycythemia
;
genetics
3.Conjugated effects of fluoride and aluminium on rat whole blood zinc, iron, calcium, magnesium and copper level
Fei, MO ; Shu-hua, XIA ; Shi-jun, WANG ; Mao-juan, YU ; Hua, CUI ; Ji-rong, CHEN ; Hua-mei, CAO
Chinese Journal of Endemiology 2008;27(5):488-490
Objective To investigate the effect of excessive fluoride,aluminum on Zn,Fe,Ca,Mg,Cuin rat blood.Methods Forty eight SD rats were randomly divided into 4 groups matched with their weights:control group,high aluminum group,high fluorine group and high fluorine-aluminum group.Aluminum content in their drinking water was 0,90,0,90 mg/L respectively.Fluorine content of their feed was 5.2,5.2,106.0,106.0 mg/kg and aluminum Was 6.8,6.8,19.7,19.7 mg/kg respectively.90 days later,the level of blood Zn,Fe,Ca,Mg, Cu Was detected by the atomic absorption spectrometry.Results Compared among these groups,Zn,Fe,Mg and Cu content of the whole blood had significant difierences(F=46.25,14.74,6.10,2.93,P<0.05),while Ca content of the whole blood did not significantly change(F=2.81.P>0.05).Factorial analysis showed that excessive intake of aluminum could significantly decreased Zn,Fe,Mg content of the blood(F=42.66,5.41,7.04,P<0.05)and excessive intake of fluorine could significantly decreased Zn,Fe,Mg,Cu content of the blood(F=64.50,37.90,9.75,6.74, P<0.05).The coexistence offluorine and Muminum had interaction to the level of Zn(F=31.59,P<0.05)and did not obviously interact with other elements(F=0.91,1.63,1.51.0.00,P>0.05).Compared with the control group [(131.30 ±13.86)μmol/L,(10.24 ±1.02),(1.71 ±0.19)mmol/L,(20.43 ±4.42)μmol/L],Zn content in the high aluminum group[(90.84±9.98)μmol/L]decreased significantly(P<0.05),so did Zn,Fe,Mg content in tlle high fluorine group[(85.85 ±10.92)μmol/L,(8.49 ±0.68),(1.52 ±0.13)mmol/L],the difference being statistically significant(P<0.05)0Zn,Fe,Mg,Cu content in the high fluorine-aluminum group,being(82.82 ±11.00)μmol/L, (8.16±0.45),(1.46±0.09)mmoL/L,(15.69±2.38)μmol/L,respectively,all decreased signitlcarIdy(P<0.05). Compared with the high aluminum group[(9.43±1.09)mmol/L],Fe content of the high fluorine aluminum group[(8.16±0.45)mmol/L]decreased significantly(P<0.05).Conclusions Excessive fluoride can cause blood zn, Fe,Mg,Cu decline,so can excessive aluminum.Combination of excessive fluofine and aiuminum has 8ignificant synergic effect on the level of Zn but have rio influence on Ca.
4.Clinical Significance of S-VEGF in Patients with Esophageal Cancer
Xiu-Shen WANG ; Meng-Zhong LIU ; Jian-Hua WANG ; Nian-Ji CUI ;
Chinese Journal of Radiation Oncology 1992;0(04):-
Objective The purpose of this study was to investigate the clinical significance of serum vascular endothelial growth factor(S-VEGF)in patients of esophageal squamous cell carcinoma with the end-point of progress-free survival rate.Methods Sera from 89 patients with esophageal squamous carcinoma,who presented and treated with concurrent chemoradiotherapy or surgical resection in Cancer Center d Sun Yat-Sen University from December 2002 to May 2004,were sampled at the time of pre-treatment.30 cases of health individuals without any evidence of disease were selected as control group.For patients with surgical resection were performed with esophagectomy and extended lymphadenectomy.For patients with concurrent chemoradiotherapy comprised of cisplatin and 5-fluorouracil.The radiotherapy dose of 2 Gy per day was initiated on Day 1 of chemotherapy and continued daily for 5 days per week for 6 weeks,for a total close of 60 Gy.Two courses of chemotherapy were given during radiotherapy at 6-week intervals.The serum vascular endothelial growth factor(S- VEGF)levels were measured with a solid phase enzyme Human VEGF Immunoassay ELISA kit.The end point of this study was progress-free survival,and time was calculated from the date of diagnosis to date of relapse or last follow evaluation.Results S-VEGF level of patients with esophageal squamous cell carcinoma was significantly higher than that of heahhy controls(475.93?44.76 pg/ml vs.294.20?23.40 pg/ml;P=0.020).S-VEGF levels in patients with StageⅢand StageⅣdisease were significantly higher than those in patients with Stage I and StageⅡdisease.The 1-year progress-free survival rate d high S-VEGF group(≥475 pg/ml)was significantly lower than that of the low S-VEGF group(<475 pg/ml)(24% vs.66%;P=0.0004).The 1-year progress-free survival rate of the patients with StageⅠand StageⅡdisease was significantly higher than that of patients with StageⅢand StageⅣdisease(71% vs.29%;P=0.0015).The variables were assessed by multivariate analysis using the Cox proportional hazards model,and the results revealed that the clinical stage and the S-VEGF were first and second independent prognosis factor,respectively.Conclusions In the current study,a high S-VEGF was found to be associated with tumor progression,poor treatment response,and poor survival in patients with squamous cell carcinoma of the esophagus.
5.Reform Microbiology Experiment Teaching Methods, Enhance the Students’ Comprehensive Abilities
Yao HUANG ; Cui-Ji HUANG ; Shi-Hua WU ; Yuan-Ping RONG ;
Microbiology 1992;0(06):-
In order to cultivate the students’ abilities of thinking and practicing and enhance their comprehensive abilities in microbiology experiment, the authors try to search for some new teaching ways and assessment methods in microbiology experimental teaching and also attempt to make some improvement in the textbook and adjustment in teaching schedule so as to develop the students into specialized talents.
6.Klinefelter syndrome complicated by mediastinal teratomas and precocious puberty: a case report.
Hong-hong ZHANG ; Ji-hua CUI ; Jian-qin QI ; Mei-rui LI ; Jian-min WU ; Yu LING
Chinese Journal of Pediatrics 2013;51(8):630-630
Biomarkers
;
blood
;
Child
;
Chorionic Gonadotropin
;
blood
;
Follicle Stimulating Hormone
;
blood
;
Growth Disorders
;
etiology
;
Humans
;
Klinefelter Syndrome
;
complications
;
diagnosis
;
genetics
;
Magnetic Resonance Imaging
;
Male
;
Mediastinal Neoplasms
;
complications
;
diagnosis
;
surgery
;
Puberty, Precocious
;
diagnosis
;
etiology
;
Teratoma
;
complications
;
diagnosis
;
surgery
;
Testis
;
pathology
7.Study on Expression,Purification of GFP-SA Recombine Protein and Anchoring Carcinoma Cells
Ming-Qian ZHOU ; Xing-Mei LINLAI ; Zhi-Ming HU ; Hua SU ; Cui-Xiang XU ; Ji-Min GAO ;
China Biotechnology 2006;0(07):-
The GFP(green fluorescence protein)-streptavidin(SA) bi-functional fusion protein was generated and characterized in order to demonstrate novel platform for efficiently and durably modifying the cell surface with SA-tagged bi-functional proteins.The GFP-SA/pET24d construct was generated and expressed in BL21(DE3) host bacteria at the high level.The recombinant protein GFP-SA was purified through the Ni-NTA affinity chromatography,and then refolded.After biotinylation B16 tumor cells were modified with GFP-SA bi-functional fusion protein and then subjected to fluorescent microscopy and FACS analysis.The effect of surface modification on the viability and growth of B16.F10 tumor cells was evaluated by MTT staining.The GFP-SA recombinant fusion protein was expressed in BL21(DE3) at about 20 % of total bacterial proteins.The GFP-SA bi-functional fusion protein exhibited the bi-functionality,i.e.,SA-mediated high-affinity binding to biotinylated cell surfaces and GFP-emitted green fluorescence.The cell surface modification with GFP-SA bi-functional fusion protein did not affect the viability and growth of the modified B16.F10 tumor cells significantly.The GFP-SA bi-functional fusion protein was obtained and could be displayed efficiently on the surface of the biotinylated B16.F10 tumor cells through the specific and tight interaction between streptavidin and biotin,thus can be used as good trace protein and experimental control in the development of surface-modified tumor vaccine.
8.Study on role of Chengqi Shengxue prescription in anti-tumor and immunoregulation.
Bing-Hua TANG ; Wei CUI ; Ji-Feng WANG ; Mei-Hua YAN ; Jin LI
China Journal of Chinese Materia Medica 2008;33(3):287-291
OBJECTIVETo investigate the role of Chengqi Shengxue prescription in anti-tumor and immunoregulation and to evaluate its effect on apoptosis and T lymphocyte subsets of tumor-bearing mice.
METHODS180 ascites tumor and Lewis lung carcinoma tumor-bearing mice were used in the screening. Then 55 mice were treated randomly with the model, cyclophosphamide (30 mg x kg(-1)), or three different dosages of Chengqi Shengxue prescription (2. 4, 1.2, 0.6 g x kg(-1). After the treatment apoptosis of tumor cell and peripheral T lymphocyte subsets of tumor-bearing mice was analyzed by flow cytometry.
RESULTLewis lung carcinoma was a nsitive tumor cell line to Chengqi Shengxue prescription. Compared with the model group, significantly increased apoptosis was observed after administration of high and medium dose of Chengqi Shengxue prescription (P < 0. 05) by PI staining. Increased early apoptosis in cancer cells was observed in all experimental doses of Chengqi Shengxue prescription by Annexin V and PI double staining (P < 0.01) . The analysis of T lymphocyte subsets showed that the percentage of CD3, CD4 and CD4/CD8 ratio decreased significantly in model group when compared with the normal ones (P < 0.01), while no change was observed in CD8. In administration groups, CD3, CD4 and CD8 were significantly lower than normal ones (P < 0.01) , but CD4/CD8 ratio did not change significantly.
CONCLUSIONChengqi Shengxue prescription has selectively inhibitive effect on the growth of mouse Lewis lung carcinoma and takes an antitransfer role. Its anti-tumor effect may be owing to inducing tumor cell apoptosis. Chengqi Shengxue prescription improves cellular immune function through enhancing CD4/CD8 ratio.
Animals ; Antineoplastic Agents ; therapeutic use ; Apoptosis ; drug effects ; Carcinoma, Ehrlich Tumor ; drug therapy ; Carcinoma, Lewis Lung ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Male ; Mice ; Mice, Inbred C57BL ; Neoplasms, Experimental ; drug therapy ; T-Lymphocyte Subsets ; drug effects ; immunology
9.Intervention of nutritional status and hypoxia endurance by a nutritional supplement in young adults living at high altitude.
Jing-yu WEI ; Chang-jiang GUO ; Ji-jun YANG ; Yin-zhi XIE ; Jian-hua CUI ; Xi-zhou ZHANG ; Bao-yu LUO
Chinese Journal of Applied Physiology 2007;23(2):150-153
AIMTo investigate the effects of a nutritional supplement on nutritional status and hypoxia endurance in young adults living at high altitude.
METHODSForty healthy male young adults were recruited and randomly assigned to control and intervention groups. The nutrition survey was carried out using weighing method. The intervention group was given a nutritional supplement specifically designed for use at high altitude, while the control group was treated with a supplement made of stir-fried flour. After 20 days of supplementation, they marched from the altitude of 3700 m to 5100 m. The changes in HR, SaO2, serum concentrations of VA and VB2 and some minerals were measured.
RESULTSThe results of nutrition survey showed that the ratio of three macronutrients was not adequate and the intakes of calcium, VA and VB2 were below Chinese RNI. The serum concentrations of calcium, magnesium and VA were below normal references. The serum VB2 concentration was at the low level o f normal reference. The nutritional supplement could increase the serum concentrations of calcium, magnesium, VA and VB2, indicating an improved nutritional status. The changes in HR and SaO2 were diminished in intervention group compared with control group.
CONCLUSIONThe nutritional supplement can improve nutritional status and increase the hypoxia endurance in young adults living at high altitude.
Adult ; Altitude ; Dietary Supplements ; Humans ; Hypoxia ; prevention & control ; Male ; Nutritional Status ; Vitamins ; therapeutic use