Computers ; Klebsiella ; isolation & purification ; Staphylococcus ; isolation & purification

The procedure using affinity chromatography for purification of G6PD from rabbit erythrocytes has been developed. This method consists of three following steps: ion exchange chromatography on DEAE - Sephadex A50, precipitation with ammonium sulfate and affinity chromatography on 2'5' ADP - Sepharose 4B. The first and the second step are directly derived from the procedure of NguyÔn H÷u ChÊn. By our method, the accumulative purification of G6PD from rabbit erythrocytes was 10243 fold. A specific activity of 69.66 IU/mg protein and a final yield of 40% were obtained.
Isolation & purification ; Erythrocytes ; Chromatography

A lectin from the haemolymph clam was purified by molecular sieving on sephadex- G75 column. The products have a high purity. The purity of lectin was assessed by SDS polyacrylamide gel. Two doublets band of apparent molecular weights of respectively about 14,000-17,000 and 20,000-23,000 Dalton were revealed upon staining with Coomassie blue. Optimum-pH of the lectin from the clams is pH 6,5-7,0 and 8,5-9,0. The lectins of the clams had very different specificity for hydratecarbons and glucoproteins
isolation & purification ; Lectins ; medicine

Aims: The aim of this study was to screen lactic acid bacteria (LAB) isolates from fermented Sumbawa mare‘s milk that meet the requirements as starter cultures, and to evaluate the effect of the selected starter culture in improving the organoleptic quality of mare‘s milk fermentation. Methodology and results: The LAB isolates (13 isolates) derived from naturally fermented Sumbawa mare‘s milk were firstly screened for acidification activity. Afterwards, the selected isolates were evaluated for the starter culture criteria such as technological properties (proteolytic test, lipolytic test, and exopolysaccharide production), food safety test (hemolytic test and antibiotic sensitivity test), antimicrobial activity test. The selected culture (SC) together with yogurt starter cultures (YC) and combination between the selected isolate and a mixture of both (MC) were used to ferment fresh mare’s milk. Six LAB isolates (DB7, BC10, DC4, BC9, DC10, and BC7) were obtained from the acidification screening. Isolate BC10 was the most potential isolate as starter culture due to its ability in terms of acidification and proteolytic activity, lack of lipolytic activity, no indication of pathogenic potency, as well as able to inhibit the growth of Escherichia coli ATCC 25922. However, this isolate was resistant to antibiotics kanamycin, trimethoprim, and cinoxacin. The isolate BC10 presented 99.99% sequence similarity with respect to Lactobacillus plantarum. Conclusion, significance and impact of study The selected starter culture (isolate BC10) was able to improve the organoleptic quality of fermented mare‘s milk especially aroma compared to the other starter cultures. Therefore, L. plantarum BC10 is a potential isolate to be used as starter culture for mare’s milk fermentation.
Lactobacillales--isolation & ; purification

The phytochemical progress on Angelica sinensis (Oliv.) Diels over the past decades is summarized. Since 1970s, 165 chemical constituents, including phthalides, phenylpropanoids, terpenoids and essential oils, aromatic compounds, alkaloids, alkynes, sterols, fatty acids, and polysaccharides have been isolated or detected from the various parts of the title plant.
Alkaloids ; isolation & purification ; Alkynes ; isolation & purification ; Angelica sinensis ; chemistry ; Benzofurans ; isolation & purification ; Fatty Acids ; isolation & purification ; Oils, Volatile ; isolation & purification ; Phytochemicals ; isolation & purification ; Phytosterols ; isolation & purification ; Polysaccharides ; isolation & purification ; Propanols ; isolation & purification ; Terpenes ; isolation & purification

The extraction and separation of effective components from Chinese herb and natural plant is one of the most important processes of natural medicine production. Recently, more and more advanced techniques have been used in the field of modern Chinese medicines, for example, microwave-assisted extraction (MAE). Developed on the base of traditional extracting technology using by organic solvents, MAE is of higher extraction rate and efficiency and better extract quality, as well as few investment, simple equipment, wide adaptability, high selectivity, good fidelity and no pollution. This paper mainly reviews the action principle and characteristic of MAE and its application in the extraction of natural products such as flavonoids, glycosides, polysaccharides, terpenoids and essence. Otherwise, the developing foreground of MAE is also prospected here.
Drugs, Chinese Herbal ; analysis ; isolation & purification ; Flavonoids ; isolation & purification ; Glycosides ; isolation & purification ; Microwaves ; Plants, Medicinal ; chemistry ; Polysaccharides ; isolation & purification ; Technology, Pharmaceutical ; methods ; Terpenes ; isolation & purification ; Triterpenes ; isolation & purification

OBJECTIVETo analyze the chemical constituents of Hedyotis diffusa.

METHODSColumn chromatographies were used to isolate and purify the chemical constituents of this plant, and their structures were identified by spectral analysis and physicochemical properties.

RESULTS AND CONCLUSIONSeven compounds were isolated and identified as p-coumaric acid (I), methyl-p-coumarate (II), 2-formyl-5- hydroxymethylfuran (III), quercetin (IV), kaempferol (V), beta-sitosterol(VI) and daucosterol(VII), respectively, among which the compounds II and III were isolated from Hedyotis diffusa for the first time.

Antineoplastic Agents, Phytogenic ; isolation & purification ; Cinnamates ; isolation & purification ; Coumaric Acids ; isolation & purification ; Furans ; isolation & purification ; Hedyotis ; chemistry ; Kaempferols ; isolation & purification ; Propionates ; Quercetin ; isolation & purification

Background: Rota vaccine is used to prevent diarrhea in children under 5 years old. Two vaccines are being used in developed countries: Rotarix (GSK) and RotaTeq (Merk). Rotarix vaccine was produced from master seed G1P8 and RotaTeq vaccine was from the coordination of human rotavirus strains G1, G2, G3, G4 and cow rotavirus strain. Thanks to the help of WHO, Ministry of Health and Ministry of Science and Technology, Center for research and production of vaccines and biologicals \ufffd?Ha Noi made study of creating rotavirus master seed G4P6 for Rota vaccine production in Vietnam. Objectives: To evaluate the safety and potency of rotavirus master seed G4P6 in the laboratory and experimental animals. Subjects and method: Rotavirus master seed G4P6 (2001019203) lot 1 (MS-PL5) and lot 2 (MS-PL5) produced in 2005, preserved at -800C were determined potency by Immunofluorescence (IF) method and tested for safety on rabits and rats. Results:2 lots of Rotavirus master seed G4P6 that had been produced in Center for research and production of vaccines and biologicals \ufffd?Ha Noi had high titre and safety in the laboratory and experimental animals. Conclusion: The results were the basis of Rota vaccine production in Vietnam.
Rotavirus/ isolation & ; purification ; Rotavirus Vaccines/ isolation & ; purification ; contraindications

OBJECTIVETo isolate and identify endophytic fungi from Malus sieboldii, and detect cytotoxicity, protease inhibition and antifungal activities of their crude extracts.

METHODThe fungi were identified with the aid of morphology or Internal Transcribed Spacer (ITS) rDNA molecular methods. Fungal activities were tested by cylinder-plate, MTT and BRpNA methods, respectively.

RESULTA total of 217 endophytic fungi were isolated from M. sieboldii. Of the 22 taxa obtained, non-sporulating, Alternaria, Colletotrichum, Aspergillu, Fusarlum, Gliocladium and Cunninghamella were dominant communities. The result of the bioactivity test showed that 30 endophytic fungi displayed inhibition against at least one pathogenic fungus, and 3 and 4 showed cytotoxicity and protease inhibition, respectively.

CONCLUSIONM. sieboldii should be a potential source of bioactive endophytic fungi.

Endophytes ; isolation & purification ; physiology ; Fungi ; isolation & purification ; physiology ; Malus ; microbiology

To reveal the variation of polysaccharides and alcohol-soluble extract contents of Dendrobium officinale, the polysaccharides and alcohol-soluble extracts contents of three D. officinale strains were determined by phenol-sulfuric acid method and hot-dip method, respectively. The results showed that the contents of polysaccharides and alcohol-soluble extracts and their total content were significantly different among D. officinale samples collected in different periods, and the variations were closely related to the phenology of D. officinale. Additionally, the quality variation of polysaccharides was closely related to the flowering of D. officinale, while the alcohol-soluble extracts was closely associated to the formation and germination of buds. According to the dynamic variation of these two compounds, it is more reasonable to harvest D. officinale at biennials pre-bloom than at specific harvesting month considering polysaccharides content. It is better to harvest before the germination of buds considering alcohol-soluble extracts. While with regards to both polysaccharides and alcohol-soluble extract, it is better to harvest this plant at the period from the sprouting to pre-bloom next year.
Dendrobium ; chemistry ; Plant Extracts ; isolation & purification ; Polysaccharides ; isolation & purification