Atrial Fibrillation ; pathology ; Humans ; Peptidyl-Dipeptidase A

Atrial Fibrillation ; surgery ; Catheter Ablation ; methods ; Humans

Adult ; Ear Ossicles ; injuries ; Humans ; Joint Dislocations ; Male ; Wounds and Injuries

Objective To explore the growing development and community succession of main sarcos-aphagous insects on pig carcasses in summer indoor and outdoor environment in Shenzhen area and to estimate the postmortem interval (PMI). Methods From early May to August in 2013, in Forensic Med-ical Examination Center of Shenzhen Public Security Bureau, the main insect species and the decomposi-tion process were observed in two adult pig carcasses of simulative indoor and outdoor environment. The different decomposition stages and the community succession of insects were recorded. Results The indoor and outdoor pig carcasses showed skeleton 412.5 and 325 hours after death, respectively. The main species of flies on pig carcasses were Chrysomya megacephala, Chrysomya rufifacies and Chrysomya chani. The main species of beetles were Crecphilus maxillosus, Necrobia ruficollis, Saprinus splendens and Dermestes maculatu. The dominant species of flies in the outdoor pig carcasses obviously produced the second generations due to the effect of mass rainfall, nor in the indoor pig carcasses. Conclusion There are regular patterns on the community succession of insects on pig carcasses in summer indoor and out-door environment in Shenzhen area. The activity patterns of seven typical insects and their larva show important value for estimating PMI.

Animals ; Anti-Inflammatory Agents, Non-Steroidal ; pharmacology ; Antioxidants ; pharmacology ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Paeonia ; chemistry ; Platelet Aggregation Inhibitors ; pharmacology ; Propyl Gallate ; isolation & purification ; pharmacology

This study aimed at the molecular mechanism of Cordyceps sinensis polysaccharide-A(CPS-A) on angiotensin (Ang Ⅱ)-induced injury of L02 cells.The effect of AngⅡ and CPS-A on the proliferation of L02 cells was analyzed by MTT assay.PCR,Real-Time PCR and Western blot were also employed to determine the expression of IL-1β,AT1R,AT2R,NF-κB p65,TNFα and other inflammatory factors at mRNA and protein levels.The results showed that Ang Ⅱ and CPS-A could inhibit the proliferation of L02 cells by 1 × 10-5 mol/L and 200 μg/ mL,respectively.PCR,Real-Time PCR and Western blot showed that CPS-A could significantly down-regulate IL-1 β,TNF-α,NF-κB and AT1R.CPS-A has a good protective effect on AngⅡ-induced L02 cell injury.

OBJECTIVE: To explore the growing development and community succession of main sarcosaphagous insects on pig carcasses in summer indoor and outdoor environment in Shenzhen area and to estimate the postmortem interval (PMI). METHODS: From early May to August in 2013, in Forensic Medical Examination Center of Shenzhen Public Security Bureau, the main insect species and the decomposition process were observed in two adult pig carcasses of simulative indoor and outdoor environment. The different decomposition stages and the community succession of insects were recorded. RESULTS: The indoor and outdoor pig carcasses showed skeleton 412.5 and 325 hours after death, respectively. The main species of flies on pig carcasses were Chrysomya megacephala, Chrysomya rufifacies and Chrysomya chani. The main species of beetles were Crecphilus maxillosus, Necrobia ruficollis, Saprinus splendens and Dermestes maculatu. The dominant species of flies in the outdoor pig carcasses obviously produced the second generations due to the effect of mass rainfall, nor in the indoor pig carcasses. CONCLUSION There are regular patterns on the community succession of insects on pig carcasses in summer indoor and outdoor environment in Shenzhen area. The activity patterns of seven typical insects and their larva show important value for estimating PMI.
Animals ; Autopsy ; Cadaver ; China ; Coleoptera ; Death ; Diptera ; Environment ; Insecta/growth & development* ; Larva ; Population Dynamics ; Postmortem Changes ; Seasons ; Swine

Objectives: To analyze the electrophysiological mapping characteristic and evaluate the effect of radiofrequency ablation in atrial tachycardia (AT) originating from the non-coronary aortic cusp (NCC). Methods: Data from 11 patients with AT originated from NCC and underwent electrophysiological mapping and radiofrequency ablation under the guidance of three-dimensional mapping system were analyzed. The electrical anatomical model of right atrium, His bundle and the root of the aorta was constructed during the procedure to measure the distance between the earliest atrial activation and His bundle. Ablation was performed at the earliest atrial activation site. Results: Cardiac electrophysiological examination evidenced the focal originating of AT in all 11 cases, right atrial activation mapping showed that all the earliest activation site was located in the left or left upper posterior of His bundle, activation time was (21.0 ± 7.9) ms prior to coronary sinus proximal reference wave A, the distance between the earliest activation site and the His bundle was (6.9 ± 3.4) mm. Aortic root mapping showed that the earliest activation sites were all located in the NCC, activation time was (35.0 ± 8.6) ms prior to coronary sinus proximal reference wave A, the distance between the earliest activation site and His bundle was (7.3 ± 4.6) mm. AT in all 11 patients were terminated after ablation, Post ablation, AT could no longer be induced by repeated stimulations. No atrioventricular conduction block occurred during and after operation. No AT recurrences were observed in all 11 patients during the 6 months follow-up. Conclusions: The success rate and safety of three-dimensional mapping guided ablation of AT originated from NCC are high. The main point of the mapping, which is crucial for the successful ablation, is that if the earliest atrial activation site of AT is located at left or left upper posterior of His bundle, it is mandatory to perform aortic root mapping and to routinely determine whether NCC is the earliest activation site of AT.

OBJECTIVETo observe the effect of Folium Panax quinquefolium saponins (PQS) on apoptosis of cardiac muscle cells (CMCs) and apoptosis-related gene expression in rats with acute myocardial infarction (AMI).

METHODSFifty Wistar rats were randomly divided into 5 groups, 40 rats underwent coronary ligation of left anterior descending branch to establish AMI rats model, while to the other 10 rats in the sham-operation group, thoracotomy without coronary ligation was carried out. The AMI model rats in treated groups were treated with high-dosage PQS, low-dosage PQS, captopril respectively for 7 days, and those in the control group were treated with normal saline instead. Then, the apoptotic CMCs were labeled by TUNEL and the expression of Bcl-2 and Fas in cardiac muscle cells were determined by immunohistochemical methods.

RESULTSThe apoptotic index of CMCs in the model rats (46.48%) was significantly higher than that in the sham-operation group ( 1.03%, P<0.01 ). CMCs apoptosis rate in the low-dosage PQS group, the large-dosage group and the captopril group was 23.53 %, 17.58 % and 25.17 %, respectively, all were significantly lower than that in the sham-operation group (P<0.01). The expression of Bcl-2 gene was up-regulated and expression of Fas protein was down-regulated in the three treated groups.

CONCLUSIONPQS can inhibit CMCs apoptosis in AMI rats, downregulate Fas protein expression and up-regulate Bcl-2 protein expression, and has antagonistic effect in myocardial ischemic injury.

Animals ; Apoptosis ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Male ; Myocardial Infarction ; blood ; genetics ; pathology ; Myocytes, Cardiac ; pathology ; Panax ; Plant Leaves ; chemistry ; Proteins ; genetics ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; biosynthesis ; genetics ; Random Allocation ; Rats ; Rats, Wistar ; Saponins ; pharmacology

OBJECTIVETo investigate the effect of Qidan Liquid (QL) on myocardial angiogenesis in rats after acute myocardial infarction (AMI) and explore its possible molecular mechanism.

METHODSAMI model was established by ligating the left anterior descending (LAD) coronary artery in Wistar rats, and intervened with QL in high, medium and low doses (QLh, QLm and QL1 group), and isosorbide dinitrate (ID group) respectively. Meanwhile, the model group, the sham group and the normal group were also set up. On the 7th (d7) and 14th day (d14) after modeling, mRNA and protein expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were detected with RT-PCR and immunohistochemistry, factor VIII expression in endothelial cells was examined, and microvascular density (MVD) in ischemic myocardium was calculated.

RESULTSMVD in the model rats increased significantly, higher than that in the normal and the sham group (P < 0.01), on d7 and d14, it was lower in the model group than that in the high and medium dose of QL groups and the ID group (P < 0.05). On d7, VEGF protein expression in the model group was higher than that in the normal group (P< 0.05), equivalent to that in the other groups, on d14, it was lower than that in the high and medium dose of QL groups and the ID group (P < 0.05 or P < 0.01). On d7 and d14, the protein expression of bFGF in the model rats increased significantly, higher than that in the normal and the sham group (P < 0.01), it decreased along the time progress between the two time points (P < 0.05), and lower than that in the high dose of QL group and ID group (P < 0.01). The mRNA expressions of VEGF and bFGF were higher in the model group than those in the normal and sham group, but lower than those in all the treated groups on d7 and d14. Comparison of the strips on d7 and d14 among various groups showed that the bFGF mRNA expression in the model group showed a descending trend as the time goes on, but in all the treated groups it was unchanged, while the change of VEGF mRNA expression didn't show any definite tendency.

CONCLUSIONQL could up-regulate the protein and gene expressions of VEGF and bFGF persistently and increase MVD in ischemic myocardium to promote the development of collateral circulation in AMI rats.

Angiogenesis Inducing Agents ; therapeutic use ; Animals ; Coronary Circulation ; drug effects ; Drugs, Chinese Herbal ; therapeutic use ; Male ; Myocardial Infarction ; drug therapy ; Neovascularization, Physiologic ; drug effects ; Phytotherapy ; Rats ; Rats, Wistar ; Solutions