Objective:To study the relationship between smoking and P53 protein expression in oral tumor tissue.Methods:The expression of P53 protein in oral tumor tissue was detected in 22 smoking cases and 32 nonsmoking cases by immunohistochemical SP method. SAS software was used for statistic analysis.Results:The positive expression rate of P53 protein in smokers (90.91%) was higher than that in nonsmokers (46.88%,P0.05). Conclusion:Smoking may stimulate the mutation of p53 gene and play an important role in the carcinogenesis of oral tissues.

Objective:To study the genetic toxic effect of smoking on human oral mucosa,and the DNA damage of exfoliated oral mucosa cells.Methods:The DNA damage of exfoliated oral mucosa cells was investigated by single cell gel electrophoresis(SCGE)in 12 cases of malignant tumor,19 cases of benign tumor and 10 health controls.There were 24 smokers and 17 non-smokers among them.The tail length and frequency of comet cells were used to measure DNA damage.SPSS and SAS software were used for statistical analysis.Results:Malignant tumors had a longer tail length and higher frequency of comet cells than benign tumors and health controls,and the difference was statistically significant(P0.05).The DNA damage of exfoliated oral mucosa cells in smokers was more serious than that of non-smokers(P

Objective To optimize the experimental condition for CO 2 supercritical fluid extraction(SFE)of volatile oil from Marchantia convoluta andto analyse the extract.Methods Orthogonaltestwas applied.SFEtechnology was usedto optimize the extractionconditionfor volatile oilfrom Marchantia convoluta and the solventextractionwas useda s the control.The extract was analyzed by GC-MS and HPLC.Results The optimal extraction conditions f or the volatile oil were:pressure at15MPa,heatingtemperatureat50℃,addingt hemodifier ofalcohol30mLtoextractfor2h,flowingratebeing10L /hand theabsorbefa-cient solution being alcohol .Twent y -three peaks were isolated and 19components were identified,accounting 82.61%.Conclusion SFEmethodhas theadvantages of shorterextractiontimeandhighextractionefficiencyas comparedwiththet radi-tionalsolventextraction.

Background Traction of epiretinal membrane results in macular morphologic change and visual functional impairment in eye with idiopathic epiretinal membrane (IERM),therefore figuring out their relationship is helpful for evaluation of disease prognosis.Objective This study was to observe the morphological change of macula and microstructure,and analyze the relationship between retina thickness,integrity of cone outer segment tips (COST) line in fovea and visual acuity.Methods This was a retrospective case-observational study.Fifty-six consecutive cases diagnosed as IERM in Zhongshan Ophthalmic Center from March 2011 to December 2011 were enrolled in this study,and all the patients showed unilateral IERM with the normal fellow eyes.Sixteen patients were males and 40 were females,with a mean age of (61.05 ± 6.58) years old.Spectral-domain optical coherence tomography (SD-OCT) examination was performed on the eyes,and Macular Cube (512×128) and HD 5 Line program were selected.Mean retinal thickness of central area (＜1 mm diameter),inner ring area (1-3 mm diameter) and outer ring area (＞3-6 mm diameter) of macula and the status of COST line (intact or fractured) was recorded.Mean thickness of whole macular areas and the difference of foveal microstructure were compared,and the correlation between retinal thickness and visual acuity was analyzed using Pearson linear correlation analysis.Related parameters including age,visual acuity,retinal thickness were also compared between the continuous COST lines group and the fractured COST lines group by independent sample t test.Results The flatted or disappeared fovea was seen in IERM eyes on the SD-OCT image.Retinal thicknesses were (446±89)μm,(418±64)μm and (328±34)ttm in the central area,inner ring area and outer ring area of macula in the IERM eyes,exhibiting significant increasing in comparison with (250±22) μm,(319±17) μm and (279±17) μm in the normal fellow eyes (t=13.370,9.523,7.769,all P =0.000).Significantly negative correlations were found between the visual acuity and the central macular thickness,inner ring thickness or outer ring thickness (r=-0.686,-0.653,-0.417,P＜0.05).In the IERM eyes,COST band was intact in 20 eyes and lack in 36 eyes.Compared with COST band intact group,aging,worse vision and increased retinal thickness were seen in the COST band absent group (t =2.109,P =0.039 ; t =-4.093,P =0.000 ; t =6.669,P=0.000;t=5.376,P=0.000;t=4.247,P=0.000).COST band was clear in all the normal fellow eyes on the SD-OCT image.Conclusions Increase of macular thickness and disruption of COST band reflect the visual function damage in IERM eye.Deficiency of COST band on OCT image seems to be an early indication of photoreceptor damage.Incomplete fovea COST band is often seen in older patients.

OBJECTIVE To investigate the existence of genes for beta-lactam antibiotic resistance and for aminoglycosides modification enzymes(AMEs) in Pseudomonas aeruginosa(PAE) isolates from ICU patients and analyze the homology among strains.METHODS ?-Lactamase genes including TEM,SHV,OXA-10,PER,VEB,GES,CARB,IMP,VIM,SPM,GIM,DHA,FOX,MOX and oprD2,were detected by PCR amplication in 21 PAE isolates.The genes for AMES including aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰwere determined by PCR amplification as well.RESULTS Among 21 isolates 21(100%),2(9.5%),1(4.8%),2(9.5%)and 4(19.0%) were positive for TEM,SHV,GES,CARB and VIM genes,respectively.The deletion of oprD2 gene was found in 14 out of 21 strains.Other ?-lactamase genes were absent in all isolates.As for AME genes,aac(3)-Ⅱ,aac(6″)-Ⅰ,aac(6)-Ⅱ,ant(3″)-Ⅰ,ant(2″)-Ⅰ and aac(3)-Ⅰgenes were present in 19.0%,23.8%,9.5%,4.8%,and 19.0% of 21 isolates,However,aac(3)-Ⅰ gene was no position in any isolates.CONCLUSIONS P.aeruginosa carries various beta-lactamase and AME genes in ICU patients.Genetic cluster analysis suggested that clonal propagation result in nosocomial infection of PAE.

Objective To evaluate the diagnostic value of conventional ultrasound and ultrasonic elastography (UE) in differentiating benign and malignant hepatic lesions.Methods One hundred and forty-five patients with 159 hepatic tumors were included in the study.Conventional ultrasound scan and UE were performed on each patient.Pathological results were as the golden standard.The diagnostic performance of the 2 methods were evaluated with receiver operating characteristic (ROC) curves and the interobserver agreements were analysed.Results A better interobserver agreement was achieved in UE (κ =0.325 at conventional ultrasound versus κ =0.782 at UE).The area under ROC curve of conventional ultrasound,ultrasonic elastography and the combination of both methods were 0.76,0.77 and 0.87,respectively.After the combination of the 2 methods,the sensitivity and accuracy could be enhanced significantly ( P ＜0.05).Conclusions Conventional ultrasound combined with UE may be more helpful in differentiating malignant from benign hepatic tumors.

Objective To analyze the distribution and clinical characters of cyanotic congenital heart diseases in newborns.Method We examined the suspicious cases with color doppler ultrasonic cardiogram(CFM),and compared with the results of autopsies.Results The most common congenital diseases in the group were complete transposition of the great arteries,with the proportion of 34.4%, and most of them were compounded cardiovascular disorders. The most common symptom was cyanosis, took the proportion of 84.3%; some patients had no cyanosis, tachypnea and murmurs.The cases with cardiovascular disorders most were combined with type Ⅱrespiratory failure and metabolic acidosis.Conclusions There are many types of congenital heart diseases, the symptoms are untypical,so the suspicious cases shall examine with CFM as early as possible.

Objective To explore the influence of helicobacter pylori(Hp) on gastric motor function in children with chronic gastrictis.Methods Forty-seven children with chronic gastrictis were classified into Hp positive and negative groups according to Hp and histology exa-mination.Each child underwent gastric emptying test of solid by using nuclide method and electrogastrogram(EGG) examination.These cases with Hp-positive acceptted these triple eradication therapy[clarithromycin 15 mg/(kg?d),amoxicillin 50 mg/(kg?d),omeprazole 0.8 mg/(kg?d)]for 2 weeks,and underwent EGG examination again after eradicating Hp.Results The half emptying time(GET_1/2)in Hp-positive gastric group were significantly longer than those in Hp-negative group(t=6.403 P

Objective To explore the risk factors in children with epilepsy and their effects on attack rate of epilepsy.Methods One hundred and sixty epilepsy patients(patient group,88 boys and 72 girls)and 150 healthy children(control group,72 boys and 78 girls)were selected.All children conformed epilepsy at the west China second hospital were consecutively included in the study for 6 months period.The range of age was from 1 month to 16 years[(7.0?4.7)years old] of patient group children.All children with epilepsy had no-causation seizure for more than twice time and were diagnosed by electroencephalogram.Neurologically normal children in same period,matched for age and sex,visiting the health care clinic were selected as controls.The range of age was from 2 month to 16 years [(6.3?4.5)years old] of control group children.The risk factors examined were febrile convulsions,head trauma,central nervous system infections,abnormal perinatal history,family history of epilepsy and parental consanguinity.The data of patients and controls were obtained from a questionnaire through personal interviews.Details on the patient,family history,and parental age at the time of childbirth were included.Medical records were then reviewed.According to the data type,the statistics were performed with ?2 test and the significance level was the P