1.Purification and Characterization of Lipases from Rhizopus chinensis
Zhen-Hua RUAN ; Dong WANG ; Yan XU ;
Microbiology 1992;0(06):-
Two lipase active fractions Lip1 and Lip2 were purified from the cell extract of Rhizopus chinensis CCTCCM201021.Both gave a single band on SDS-PAGE after using ammonium sulfate precipitation、Phenyl-Sepharose FF、DEAE-Sepharose FF and Sephadex G100 gel filtration chromatographies.The molecular masses of two lipases were 59.2kD and 39.4kD respectively.Lip1 and Lip2 showed optimal pH at 8.0 and 8.5 and their optimal temperatures were 40℃ and 35℃ respectively.The substrate specificity of the two lipases was obviously different.Lip1 was more specific to long chain fatty acid of p-nitrophenyl esters while Lip2 had a preference for the hydrolysis of short chain fatty acid of p-nitrophenyl esters.Lip1 had 1,3-position specificity for triacylglycerols hydrolysis while Lip2 had nonspecific position.Both lipases were stimulated by Ca 2+、Mg 2+ while SDS had strong inhibition on their activities.Lip1 and Lip2 had good stability in cyclohexane、hexane、heptane and isooctane(30% V/V).
2.Study on Vancomycin-resistant Enterococci Carried by Intestinal Tract in Different People Groups
Microbiology 1992;0(03):-
512?g/mL; MIC of 16 VIE to vancomycin were 16?g/mL; MIC of 8 VIE to vancomycin were 8?g/mL. It is a risk factor for hospital infection that VRE carriage of inpatients in intestinal tract is high. There is 100% agreement be-tween phenotypes and genotypes in 46 vancomycin resistance enterococci. VRE are multiresistant. Part iso-lates have high homology.
4.ALK-positive primary anaplastic large-cell lymphoma of stomach: report of a case.
Wei-hua WU ; Hong-ying CAO ; Zhen XU
Chinese Journal of Pathology 2011;40(3):197-198
Adult
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Diagnosis, Differential
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Female
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Gastrectomy
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methods
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Gastrointestinal Stromal Tumors
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metabolism
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pathology
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Humans
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Ki-1 Antigen
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metabolism
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Leukocyte Common Antigens
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metabolism
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Leukosialin
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metabolism
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Lymphoma, Large-Cell, Anaplastic
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metabolism
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pathology
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surgery
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Melanoma
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metabolism
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pathology
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Mucin-1
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metabolism
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Receptor Protein-Tyrosine Kinases
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metabolism
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Stomach Neoplasms
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metabolism
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pathology
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surgery
5.Pineal choriocarcinoma: report of a case.
Hong-Ying CAO ; Wei-Hua WU ; Zhen XU
Chinese Journal of Pathology 2010;39(11):774-775
Antibodies, Monoclonal, Murine-Derived
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metabolism
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Brain Neoplasms
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metabolism
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pathology
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Child
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Choriocarcinoma
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metabolism
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pathology
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Chorionic Gonadotropin, beta Subunit, Human
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metabolism
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Diagnosis, Differential
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Humans
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Male
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Neoplasms, Germ Cell and Embryonal
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metabolism
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pathology
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Pineal Gland
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pathology
7.Mid-term outcomes of Bryan cervical disc arthroplasty versus anterior cervical discectomy and fusion for cervical spondylopathy
Dingjun HAO ; Baorong HE ; Zhengwei XU ; Hua GUO ; Zhen CHANG
Chinese Journal of Orthopaedics 2011;31(1):18-23
Objective To compare the clinical outcomes of Bryan disc replacement with anterior cervical discectomy and fusion (ACDF) in patients with cervical spondylopathy. Methods Sixteen patients underwent Bryan cervical disc replacement (A group), and 35 patients underwent traditional ACDF (B group) were included in the study. Patients were followed up at regular intervals. The JOA score, SF-36, neck disability index (NDI) score and the dynamic flexion-extension radiographs were used to evaluated the oucomes.Results All the patients were followed up for more than 6 years (mean, 73.5 months). There were no severe adverse events in both groups. In A group, there were no differences between postoperative and preoperative mobility of surgical segments (P>0.05). All patients obtained bone fusions 6 month after surgery in group B.In both groups, the clinical symptoms relieved obviously after surgery. The postoperative scores of the JOA,SF-36 and NDI significantly improved compared with those of preoperative ones (P<0.05). In B group, range of motion (ROM) was significantly decreased postoperatively (P <0.01); in A group, there were no significant differences between postoperative and preoperative ROM (P>0.05). The difference between two groups regarding ROM was noted (P<0.05). Conclusion The mid-term outcomes of Bryan cervical arthroplasty are satisfied. And the cervical arthroplasty which can maintain the mobility of the segment, and decrease the incidence of the postoperative neck axial symptoms is a viable alternative to cervical spondylopathy.
8.Research Advance in Environmental Estrogens of Effluent from Sewage Treatment Plants
Hai-Zhen ZHANG ; Guang-Hua LU ; Shuai XU ;
Journal of Environment and Health 2007;0(08):-
The conception,sources and research focus on environmental estrogens of effluent from sewage treatment plants were briefly introduced and the advances on environmental estrogens of effluent from sewage treatment plants were reviewed in the present paper.The study and applications of biomarker-vitellogenin (Vtg) was also discussed.The future research direction of environmental estrogens was proposed.
9.Pathomorphological change of the atlanto-occipital segment of vertebral artery related with cervical vertigo.
Bing-hua FAN ; Li XU ; Min LIN ; Wei LI ; Fang-jun WANG ; Quan-zhen XU
China Journal of Orthopaedics and Traumatology 2015;28(1):39-42
OBJECTIVETo explore the pathomorphological change of the atlanto-occipital segment of vertebral artery (V3 part) related with cervical vertigo.
METHODSFrom June 1999 to November 2011, the pathomorphological change of the atlanto-occipital segment of vertebral artery were observed in 1680 patients with cervical vertigo using 3D-CTA technology. The clinical data of these patients were analyzed. There were 783 males and 897 females, aged from 22 to 70 years old with an average of 52.8 years old. Doppler examination showed vertebral basilar artery flow velocity to speed up or slow down.
RESULTSThe blood vessel of 3360 branches were detected in 1680 patients and 2778 branches were detected out vascular anomaly. And 829 branches were in V1 segment, 421 were in V2, 328 were in V3, 1190 were in V4. The pathomorphological changes in the atlanto-occipital segment (V3) of vertebral artery included angiospasm, congenital absence, abnormal exit, localized stenosis.
CONCLUSIONThere are 4 kinds of pathomorphological changes in the atlanto-occipital segment of vertebral artery related with cervical vertigo. The 3D-CTA result can be used to judge prognosis and adopt reasonable treatment for the patients.
Adult ; Aged ; Atlanto-Occipital Joint ; Cervical Vertebrae ; Female ; Humans ; Male ; Middle Aged ; Tomography, X-Ray Computed ; Vertebral Artery ; diagnostic imaging ; pathology ; Vertigo ; pathology
10.Advance on chemical compounds of Ainsliaea genus.
Fa-jin FENG ; Zhi-ling XU ; Qian-jun ZHANG ; Zhen-hua YIN ; Wen-yi KANG
China Journal of Chinese Materia Medica 2015;40(7):1244-1251
Plants in Ainsliaea genus, belongs to Compositae family, are traditional Chinese medicine and widely used in folk. These plants contain various types of chemical components, and main components are sesquiterpene lactone and its glycosides. In addition, there are triterpenoids, flavonoids, steroids, phenolic acid, long chain fatty acid and volatile oils. Recently, much attention has been payed to varlous research of A. fragrans. This paper reviewed and summarized the chemical components to provide the theoretical basis for the use of Ainsliaea.
Asteraceae
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chemistry
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Molecular Structure