Objective To determine the relationship of bone mineral density (BMD) with age and sex hormone in elderly men. Methods The BMD in orthotope lumbar vertebra, femur neck, greater trochanter, Wards triangle region and shaft of femur were measured by dual-energy X-ray absorptiometry (DEXA), and serum testosterone (TT) and estradiol (E2) were determined by chemiluminescence in 360 elderly men. According to age and BMD, the elderly men were divided into several groups. The differences in BMD, serum TT and E2 among the groups were compared. Results There were 48 cases in osteoporosis group, 72 cases in bone mass reduction group and 240 cases in normal control group. The BMD in femur neck, greater trochanter of femur, Wards triangle region and shaft of femoral were decreased with ageing (F=3. 038,3. 029,3. 024,3. 021, respective, all P<0.05). In>80 age group, the BMD in femur neck, greater trochanter of femur, Wards triangle region and shaft of femoral were (0. 70±0. 140), (0. 682±0. 185), (0. 629±0.211), (0. 986± 0. 160)g/cm2, respectively;In 70-80 age group, they were (0. 829±0. 156), (0. 765±0. 170), (0. 698±0.187), (1. 042±0. 190) g/cm2, respectively;In 60-70 age group, they were (0. 875± 0. 138), (0. 800±0. 130), (0. 731±0. 145), (1. 071±0. 125)g/cm2, respectively. The differences in BMD of L1-4 had no statistical significance among different age groups(F=2. 988, P>0.05). There was no difference in level of serum TT among normal control group, bone mass reduction group an dosteoporosis group (F=3. 032, P>0. 05), while the level of serum E2 in the three groups were (180. 6±62. 3), (130. 5±39.9) pmol/L and (110. 5±68. 5) pmol/L, respectively. Bone mass was decreased with the decreased serum E2 level(F=3. 059,P<0. 05). Conclusions BMD is declined with ageing, and the level of serum E2 may affect the occurrence of osteoporosis in elderly men.

Objective To investigate insulin-like growth factor-1(IGF-1) on the protective effect of infant myocarditis.Methods The serum levels of IGF-1 was detected by two-site enzyme-linked immunosorbent assy ( ELISA) and CK-MB by electrochemiluminescence at the acute and convalescent in 50 infant with myocarditis,and 50 normal healthy children as the controls.Results The serum levels of IGF-1 in acute myocarditis was significantly lower than those of healthy children and the convalescen[(22.90 ±9.30) mg/L vs (45.69 ±10.95) mg/L, (38.99 ±9.06)mg/L,all P<0.01],while the CK-MB was significantly higher than those of the control group and the convalescent[(77.48 ±8.71)mg/L vs (14.99 ±7.62)mg/L,(17.99 ±6.31)mg/L,all P<0.01.IGF-1 and CK-MB was negative correlation (r=-0.62,P<0.01).Conclusion IGF-1 is involved in the repair process of myocardial injury in myocarditis infant,and play some protective role in the mechanism.

Objective To investigate the psychology of the folks of the mental disabled after traffic accidents,and to survey the influence of the coping style and social support on their psychology.Methods 96 folks of the mental disabled after traffic accidents were evaluated with Symptom Checklist-90(SCL-90),Trait Coping Style Questionnaire(TCSQ)and Social Support Rating Scale(SSRS).Results The total score and every factor's score of SCL-90 were all higher than norms(P<0.01).The total score and every factors' score of SCL-90 negatively correlated with positive coping style and objective social support,and positively correlated with negative coping style(P<0.05 or P<0.01).Conclusion The folks of the mental disabled after traffic accidents show more psychological problems,that correlate with the coping styles and social support.

Aged ; Humans ; Male ; Middle Aged ; Mycoses ; complications ; Nasal Septal Perforation ; complications ; microbiology ; Sinusitis ; complications ; microbiology

Objective To evaluate chest CT features of microscopic polyangiitis(MPA).Methods Clinical data and chest CT images of 66 patients diagnosed as MAP had been collected.Depending on clinical manifestations.the 66 cases were divided into the active group and the stable group.The data of two groups were analyzed by x2 test.Results Forty-three of 66(65.2%)CT images were abnormal including 32 of them in active group and 11 in stable group. In these patients,CT images showed ground glass opacities(53.5%),lung consolidations(23.2%),scattered patchy opacities(44.2%),ground glass opacities and/or lung consolidations(76.7%),reticular opacities(65.1%),honeycomb(14.0%),pleural effusions(48.8%),emphysema(18.6%),and mediastinal adenopathies(58.1%).Lung consolidations,ground glass opacities,scattered patchy opacities,mediastinal adenopathies,and pleural effusion in the active group are more common than that in the stable group(X2=4.479,4.083,4.053,9.697,9.345 respectively,P<0.05).Conclusion Chest CT features of in patients with MPA have difference between active and stable periods.The chest CT scan is a useful tool to diagnose MAP in different periods and guide the treatment.

AIM:To investigate the relationship of clinical efficacy,blood trough concentration and adverse reavtion,which in the treatment of methicillin-resistant Staphylococcus aureus infection with Vancomycin.METHODS:The clinical data of 82 hospital patients who were infected by methicillin-resistant Staphylococcus aureus and treated with Vancomycin from January 2008 to October 2009 were retrospectively analyzed the clinic efficacy,bacteriological treatment,adverse reaction were observed,and the blook trough concentration was detected.RESULTS:The treatment effective rate of Vancomycin-blood trough concentration which was less than 5 ?g/mL,in the range of 5-10 ?g/mL,and greater than 10 ?g/mL were 68.75%,72.73% and 81.82%.The bacterial clearance rates were 62.50%,68.18% and 72.73%.One case of blood trough concentration of 14.6 ?g/mL in patients with renal toxicity.CONCLUSION:Increasing the blood trough concentration of Vancomycin can improve the treatment of methicillin-resistant Staphylococcus aureus infection in the efficacy and bacterial eradication rates,but the probability of renal toxicity adverse reactions will also increase accordingly.

Objective To investigate the clinical effect of early rehabilitation treatment on patients with severe traumatic brain injury (sTBI).Methods Forty sTBI patients were divided into treatment group (n =20) and control group (n =20) according to the random number table.Conventional treatment was performed on all patients including dehydration to decrease intracranial pressure,hemorrhage control,neurotrophic treatment,antiinflammation therapy,and gastric acid control.In addition to these interventions,patients in treatment group received hyperbaric oxygen treatment,median nerve stimulation,fastigial nucleus stimulation,and bedside motor therapy in the early period.Intracranial pressure and partial pressure of brain tissue oxygen (PbtO2) were continuously monitored during the process of treatment.GCS was measured before and 15 days after treatment and single-photon emission computed tomography (SPE-CT) was used to evaluate cerebral perfusion.Results There was no statistical difference between the two groups with respect to GCS in advance of treatment (P ＞ 0.05),but GCS differed between treatment group and control group after treatment [(10.18 ± 3.75) points vs (8.33 ±2.36) points,P ＜0.05],with substantial improvement in treatment group.Significantly improved cerebral perfusion was seen in treatment group.On day 5 after treatment,intracranial pressure in treatment group lowered significantly compared with that in control group (P ＜ 0.05).On day 6 after treatment,PbtO2 was significantly higher in treatment group than in control group (P ＜ 0.05).Conclusion Early rehabilitation treatment leads to improved outcome and acts a positive effect on nerve function recovery.

To prokaryotic express prokaryotically and to purify the efaA protein from Enterococus faecalis so as to provide the basis for the further study on the pathogenesis and clinical sero-diagnosis of endocarditis caused by E.faecalis, efaA gene of E.faecalis was amplified by PCR, the PCR-amplified product was digested with restriction enzymes and cloned into prokaryotic vector pET32a to construct the recombinant plasmid pET30a/efaA. This recombinant plasmid was confirmed by double enzyme digestion with BamhI and Xhol and then subjected to sequencing, and transformed to E.coli BL21 (DE3). Expression of the fusion protein was induced by IPTG, and analyzed by SDS-PAGE and Western blotting. The recombinant fusion protein was purified by His-binding affinity chromatography.It was shown that efaA gene of 943 bp in size was amplified from Enterococus faecalis and the recombinant plasmid pET30a,/ efaA was successfully constructed and expressed in E.coli BL21. The purified product was found to be 34 kDa in molecular weight as demonstrated by SDS-PAGE and Western blotting. It is evident that the efaA protein of E.faecalis can be successfully expressed and purified.

Objective To explore the value of ectopic gastic mucosa imaging in diagnosis of Meckel′s diverticulum.Methods Twenty-five cases suspected of gastrointestinal bleeding underwent conventional ectopic gastic mucosa imaging with 99mT_cO_4-.The diagnostic criteria Meckel′s diverticulum was that abnormal radio active accumulation in the abdomen observed.Results Three cases of ectopic gastic mucosa imaging was positive,and the diagnosis was all confirmed by surgery and pathology examination.The rest 22 cases were caused by other reasons confirmed by affirmative examinations.Conclusions Ectopic gastic mucosa imaging is easy to conduct,safe with no injury to the patients.It can be the first choice of examination in the diagnosis of gastrointestinal bleeding in children.

Actin cytoskeleton in podocyte is a complicated network structure,and the stability of this structure depend on many proteins which located in slit diaphragm,the apical membrane domain and the basal membrane domain with the stimulus of mechanical stress,the actin cytoskeleton can be adaptive regulated to maintain the normal function of glomerulus,and several signal pathways involve in the process,such as RhoA/Rho kinase signal pathway and TRPC6.