Objective To compare the ocular parameters measured by 2 biometry devices Tomey OA-2000 and IOLMaster in cataract eyes.Methods A total of 74 cataract patients (74 eyes) were included in this prospective study.Ocular parameters,including axial length (AL),keratometry (K),anterior chamber depth (ACD) and white to white (WTW),were obtained from Tomey OA-2000 and IOLMaster,respectively.Paired t-test was applied to analyze the differences of ocular parameters from Tomey OA-2000 and IOLMaster,while Bland-Altman plot was used to assess the agreement of these ocular parameters.Results The AL,flat K,steep K,mean K,central corneal thickness (CCT),ACD,lens thickness,pupil diameter and WTW values measured by Tomey OA-2000 were (24.26 ± 2.71) mm,(44.09 ± 1.76) D,(45.01 ± 1.73) D,(44.55 ± 1.72)D,(518.41 ±34.18) μm,(3.07 ±0.48)mm,(4.48 ±0.61)mm,(4.07 ±0.95)mm and (11.50 ± 0.52)mm respectively.Values of AL,flat K,steep K,mean K,ACD and WTW from IOLMaster were (24.33 ± 2.69) mm,(44.21 ± 1.74) mm,(45.15 ± 1.76) D,(44.69 ± 1.74) D,(3.04 ± 0.46)mm and (11.65 ± 0.41) mm respectively.The difference values of AL,flat K,steep K,mean K,ACD and WTW between Tomey OA-2000 and IOLMaster were (-0.07 ±0.10) mm (t =-5.99,P＜0.01),(-0.12 ± 0.21) D (t =-4.14,P＜0.01),(-0.14 ± 0.20)D (t =-5.88,P＜0.01),(-0.12 ±0.13) D (t =-7.44,P＜0.01),(0.03 ±0.14) mm (t=1.99,P=0.05) and (-0.14±0.32) mm (t=-3.73,P＜0.01) respectively.The 95％ limits of agreement (LoA) of the AL,flat K,steep K,mean K,ACD obtained from the two devices was 0.27 mm,0.50 D,0.53 D,0.38 D,0.30 mm,respectively,and the agreement was good;95％ LoA of WTW was 0.78 mm,and the consistency was poor.Conclusion The differences of AL,K values and ACD between Tomey OA-2000 and IOLMaster were small,suggesting the agreement is unified.

Objective: To investigate the relationship between the autophagy and the sensitivity to DDP (cisplatin) in epithelial ovarian cancer SKOV3 and SKOV3/DDP cells. Methods: The formation of autophagosomes in SKOV3 and SKOV3/DDP cells was observed under a TEM (transmission electron microscope). MTT assay was used to screen the dose of nontoxic reaction of autophagy inhibitor 3-MA (3-methyladenine) and to examine the sensitivity to DDP in SKOV3 and SKOV3/DDP cells after treatment with the combination of DDP and 3-MA. The apoptotic rate and the expressions of autophagic marker protein LC3 (microtubule-associated protein 1 light chain 3) and autophagy-related protein Beclin1 in SKOV3 and SKOV3/DDP cells after treatment with DDP and 3-MA alone and the combination of DDP and 3-MA were detected by flow cytometry and Western blotting, respectively. Results: Much more autophagosomes in SKOV3/DDP cells were observed as compared with that in SKOV3 cells. As compared with DDP treatment alone, when DDP was used in combination with 3-MA, the IC50 (half inhibitory concentration) value of DDP for SKOV3/DDP cells was decreased from (17.71±0.15) μg/mL to (8.85±0.11) μg/mL (P < 0.05), and the apoptotic rate was increased from (6.93±0.94) % to (14.82±0.85) % (P < 0.05), as well as the expression levels of LC3 and Beclin1 proteins in SKOV3/DDP cells were significantly decreased (P < 0.05), but there were no significant changes could be observed in SKOV3 cells. Conclusion: The increased activity of autophagy is related to DDP-resistance of ovarian cancer cells. The inhibition of autophagy may enhance the sensitivity to DDP in SKOV3/DDP cells. Copyright © 2013 by TUMOR.

The notational DSA was first proposed for the application as a managing tool of neuronradiology in the early 70's of 20th centery.Later on,three dimensional image reconstruction(3D DSA) was aquired along with the progressing development of technique and equipment devices.As a rather practical instrument,it has been utilized successfully in the diagnosis and management of head and neck vascular disorders,and abdominal parenchymal diseases during recent years.We comprehensively intoduce the application of rotational DSA for the diagnosis and management in hepatic diseases.

Objective To understand the features of lymphatic vessel,and to summarize the foundation and mechanism of the promotion and inhibition of tumor lymphangiogenesis recorded on the current studies of animal ex- periments and clinical researches.Methods The related literatures of the structural features of lymphatic vessel, lymphatic endothelial molecular markers,the origin of lymphatic tumors,the molecular mechanisms and regulatory factors were reviewed,and the relationship between tumor lymphangiogenesis and lymphatic metastasis,the treat- ment targeting at the formation of the anti-tumor lymphatic vessel and its existing problems were also analyzed.Re- suits Hyperplasia of lymphatic vessels occurred during the process of tumor formation and progression.The struc- tural features of the lymphatic vessels in the tumor were conducive to tumor lymphatic metastasis.In recent years, methods of anti-lymphangiogenesis and inhibition of tumor lymphatic metastasis had achieved considerable success in animal experiments.However,there were still a lot of problems need to be solved.Conclusion Tumor lymphangio- genesis has a significantly positive correlation between tumor lymphatic metastasis and patients'prognosis,which may indicate that treatment against the formation of tumor lymphatic vessel maybe effective.

Objective To observe the changes of extracellular matrix(ECM) production and hypertrophy of glomerular mesangial cells(GMCs) induced by high glucose(HG),and the inhibitory role of myriocin(ISP-1).Methods GMC cultured with normal glucose(5.6 mmol/L D-Glucose,NG),HG(25.2 mmol/L D-Glucose) and HG plus ISP-1(100 mg/L) for different durations(0,24,48,(72 h)).The sizes of GMC were indicated by forward scatter intensity,measured by flow cytometery,and the levels of fibronection(FN),collagen Ⅳ(Col Ⅳ),laminin(LN),precollagen Ⅲ(Pcol Ⅲ) and hyaluronic acid(HA) in the supernatant of cultured GMC were detec-(ted) by ELISA.Results Compared with NG,HG could induce GMC hypertrophy(P

Evidence has indicated that low doses of anti-tumor regimens can induce cell apoptosis in vitro, although different regimens induce apoptosis by different mechanism and pathway. In recent years, new tumor treatment strategy has been mainly focused on inducing tumor cell apoptosis. The present review discusses the advantages and disadvantages of inducing tumor cell apoptosis. The benefit of inducing apoptosis is not to cause inflammatory reaction, but as its disadvantage, it inhibits immune responses, and the phagocytosis of apopotic bodies may result in horizontal transfer of genes (including oncogenes and other oncogenic materials), which can be one of the causes of tumor relapse. This paper proposes that the tumor treatment strategy should be turn into promoting tumor cell necrosis and inducing anti-tumor immune responses.
Antineoplastic Agents ; therapeutic use ; Apoptosis ; drug effects ; Humans ; Necrosis ; chemically induced ; Neoplasms ; drug therapy ; immunology ; pathology

Objective To observe the effects of long term injection sodium salicylate on the auditory brain-stem response(ABR)and expression of glutamic acid decarboxylase -67(GAD67) in rat inferior colliculus .Methods Eighteen healthy Wistar rats were randomly divided into three groups :the sodium salicylate group (intramuscular injection of 10% sodium salicylate ,175 mg/kg ,twice daliy for 28 days) ,the saline group (intramuscular injection with saline on same does at the same time) ,the control group (without any treatment) .The rats received ABR after modeling ,then were decapitated and inferior colliculus tissues were stripped .Western blot was used to study the dif-ferent expression of GAD67 protein levels in the three groups .Results Compared with the saline group and control group ,ABR thresholds of the sodium salicylate group were significantly elevated and latency of wave Ⅲ was aslo sig-nificantly prolonged(P<0 .01) ,while there was no significant difference between the saline group and the control group(P>0 .05) .The inferior colliculus GAD67 protein expression level of sodium salicylate group was significantly higher than the saline group and control group(P<0 .01) ,while there was no significant difference between the saline group and the control group(P>0 .05) .Conclusion Long term injection of sodium salicylate can cause a change in the inferior colliculus of GAD67 protein expression and the up regulation of GAD67 expression may occur as a com-pensatory response to increase inhibiting effect .The change of GAD67 protein expression is likely as a compensatory and regulatory mechanisms for sodium salicylate ototoxicity .

Objective To investigate the safety and feasibility of laparoscopic ELAPE for elderly patients with low advanced rectal cancer.Methods Totally 48 cases patients with low advanced rectal cancer surgery aged over 65 years old were analyzed retrospectively,who come from Beijing Hospital between Jan 2012 and Jan 2015.A total of 26 cases underwent Laparoscopic extralevator abdominoperineal excision (L-ELAPE) and 22 cases underwent Laparoscopic abdominoperineal excision(L-APE).Clinical data including general data,operation time,intraoperative blood loss,complications,pathological data,postoperative in hospitalization were retrospectively analyzed in patients.Results The mean operation time between L-ELAPE and APE group was (312±46)min vs.(245±62)min,mean intraoperative blood loss was(170±74)ml vs.(250± 109)ml,Operative complications was 26.9％ vs.27.3％,harvested lymph node was (16.0 ± 5.8)cm vs.(15.0±7.2)cm,intraoperative bowel perforation(IOP)rate was 0％ vs.18.2％,CRM involvement was 3.8 ％ vs.13.6 ％,mean postoperative hospital stay (days) was (13.1 ± 4.6) d vs.(13.7 ± 6.1) d.The mean operating time of L-ELAPE group was longer and mean intraoperative blood loss was much less than APE group,IOP rate and circumferential resection margin(CRM)involvement were higher in APE group(P＜0.05).Conclusions L-ELAPE is a safe and feasibility alternative approach for elder patients with rectal cancer.It is related with less intraoperative blood loss,IOP rate,CRM involvement and longer operating time contrast with L-APE.

Objective To investigate the effect of pronuciferine on apoptosis of cultured human umbilical vein endothelium cells(HUVECs) induced by angiotensin Ⅱ(AngⅡ).Methods HUVECs cell line ECV304 was cultured in vitro,pretreated with Captopril(10 ?mol/L) or pronuciferine 10,1,0.1,0.01 ?mol/L for 30 min,respectively,then treated with AngⅡ(1 ?mol/L).Cell-morphosis was observed by light microscope.Cells viability was assessed by MTT assay.Production of nitric oxide(NO),activities of total nitric oxide synthase(tNOS),and inducible nitric oxide synthase(iNOS) were measured by colorimetry.Apoptosis rate was measured by Flow Cytometer(FCM).Results AngⅡ induced typical endothelial cell apoptosis and the apoptosis rates were significantly higher than those of the control group((P