1.Effect of hypoxia on the expression of connective tissue growth factor in lung fibroblast
En-Hai CUI ; Feng HUA ; Yong LIN ;
Chinese Journal of Rheumatology 2001;0(04):-
Objective To observe the effects of hypoxia on the expression of connective tissue growth factor(CTGF)and matrix metalloproteinase 9(MMP-9)in lung fibroblasts at different time points.Methods Fibroblasts were cultured at hypoxic condition 0,1.5,3,6,12 h respectively.CTGF mRNA and MMP-9 mRNA level were detected in each group by reverse transcription-polymerase chain reaction(RT-PCR).The concen- tration of CTGF and MMP-9 protein in fibroblasts supernatant were determined using enzyme-linked im- munosorbent assay(ELISA).Results Hypoxia stimulated fibroblasts increased the level of CTGF mRNA with- in 1.5 h,and the levels remained at a plateau up to 6 h,and then decreased by 12 h.The level of MMP-9 mRNA increased significantly within 3 h,and the levels kept the trend for increasing.ELISA revealed that the levels of both CTGF and MMP-9 protein/cell in medium conditioned by fibroblasts exposed to hypoxia were maximal at 12 h.The level of MMP in the CTGF-Ab treated groups was significantly decreased compared to the untreated groups.Conclusions These findings suggest that hypoxia stimulates fibroblasts to release CTGF as a mitogen factor,which initiates the fibrosis cascade and airway remodeling by regulating the balance of extracellular ma- trix synthesis and degradation via MMP-9 which is secreted by fibroblast cells in response to CTGF.
2.Clinical study on relationship between protein tyrosine kinase JAK2 V617F mutation and high altitude polycythemia.
Lin-hua JI ; Zhan-quan LI ; Sen CUI
Chinese Journal of Hematology 2012;33(4):319-320
Adult
;
Aged
;
Altitude
;
Humans
;
Janus Kinase 2
;
genetics
;
Male
;
Middle Aged
;
Point Mutation
;
Polycythemia
;
genetics
3.Flow Injection Analysis of Tannic Acid withEnhanced Chemiluminescent Detection
Shifeng LI ; Hua CUI ; Yugang SUN ; Xiangqin LIN
Chinese Journal of Analytical Chemistry 2001;29(3):333-335
A novel flow injection chemiluminescent enhancement method for the determination of tannic acid based on luminol-KIO4-Mn2+ system is developed. The method is simple, rapid and effective to determine tannic acid in the range of 3.0×10-8~5.0×10-6 mol/L with a detection limit of 9×10-9 mol/L. The relative standard deviation is 1.5% for the determination of 1.0×10-6 mol/L tannic acid (n=11). The mehtod has been applied to determine the content of tannic acid in Chinese gall with satisfactory results.
5.Nursing of 5 patients with liver transplantation complicated with early portal vein thrombosis treated with post-embolectomic anticoagulation
Yuan WANG ; Jianyu LIN ; Cen CUI ; Yanping GAO ; Hua FAN
Modern Clinical Nursing 2016;15(8):46-48
Objective To summarize the experience of nursing the patients with liver transplantation complicated with early portal vein thrombosis treated with post-embolectomic anticoagulation. Method The clinical data of 5 cases of liver transplantation-induced portal vein thrombosis treated with post-embolectomic anticoagulation was reviewed to summarize the curative effect , key points for disease conditions and anticoagulative nursing. Results The anticoagulative effect was not good in only one case. 4 other cases were recovered by embolectomy and postoperative anticoagulation therapy as well as related nursing measures. Conclusion The nursing measures such as close observation of the disease processing, early reasonable anticoagulation, accurate medication, prevention of patent complication are critical for preventing the recurrence of portal vein thrombosis after liver transplantation.
7.Observation on instant analgesic effect of Governor Vessel-regulating and collateral-unblocking acupuncture method for primary headache
Xiao XING ; fang Li ZHANG ; hua Lin CUI ; hua Wei LIU ; jun Yan WANG
Journal of Acupuncture and Tuina Science 2017;15(5):366-370
Objective:To observe the instant analgesic effect of Governor Vessel-regulating and collateral-unblocking acupuncture method in treating primary headache and to inherit and carry forward the academic achievements of Gao's acupuncture school in Yanzhao (Hebei Province) area. Methods:Eighty eligible primary headache patients were randomized into an observation group and a control group following their visiting sequence, 40 patients in each group. The observation group was intervened by Yanzhao Gao's Governor Vessel-regulating and collateral-unblocking acupuncture, and the control group was intervened by ordinary acupuncture method. The visual analogue scale (VAS) was used to evaluate the headache intensity before and after the first treatment session in the two groups, to compare the instant analgesic effect of the two acupuncture methods. Results:After the first treatment session, the VAS scores dropped significantly in both groups (both P<0.05). The change of VAS score after the initial treatment in the observation group was significantly different from that in the control group (P<0.05). The percentage of patients with headache completely vanished instantly after the first treatment was markedly higher in the observation group than that in the control group (P<0.05). Conclusion: The two acupuncture methods both can produce a significant instant analgesic effect in treating primary headache, while Yanzhao Gao's Governor Vessel-regulating and collateral-unblocking acupuncture method is superior to ordinary acupuncture method.
8.Surveillance on 138,923 perinatal infant chilopalatognatus cleft lip and palate in Zhengzhou.
Xiu-ping ZHOU ; Ling CUI ; Yu-lin CUI ; Hua-feng GUO
Chinese Journal of Epidemiology 2007;28(5):518-518
China
;
epidemiology
;
Cleft Lip
;
epidemiology
;
Cleft Palate
;
epidemiology
;
Female
;
Humans
;
Infant, Newborn
;
Male
9. Relationship between personality characteristics and turnover intention of medical staff in an infectious disease hospital
Kunhong MA ; Zhanyu CUI ; Lin LI ; Hua CHAO ; Ye WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2017;35(12):936-938
Objective:
To investigate the relationship between personality characteristics and turnover intention of the medical staff in an infectious diseases hospital.
Methods:
Using the cluster sampling method, a total of 366 members of medical staff were selected from different departments in an infectious disease hospital from May to August, 2013. The general information, such as sex, age, education level, and professional title, were collected and they were subjected to a survey using Cattell’s 16 Personality Factor Questionnaire and Turnover Intention Scale. The data were subjected to logistic regression analysis.
Results:
Compared with the Chinese norm, the medical staff in the infectious disease hospital had significantly higher scores of intelligence, stability, bullying, excitability, perseverance, social boldness, fantasy, privateness, independence, and self-discipline and significantly lower scores of gregariousness, sensitivity, suspicion, anxiety, and tension (
10.Utility of single fiber electromyography in the differential diagnosis of amyotrophic lateral sclerosis and cervical spondylosis
Mingsheng LIU ; Liying CUI ; Xiaoguang LI ; Yuzhou GUAN ; Lin CHEN ; Benhong LI ; Hua DU
Chinese Journal of Neurology 2009;42(8):525-528
Objective To assess the utility of single fiber electromyography (SFEMG) in the differential diagnosis of amyotrophic lateral sclerosis (ALS) and cervical spondylotic radiculopathy and myelopathy. Methods SFEMG was performed in extensor digitorum communis muscles (EDC) of 3 groups of patients, including 61 ALS patients with findings of cervical spendylosis on MRI, 59 ALS patients with normal MRI of cervical spine, and 55 patients with cervical spendylotic radiculopathy and myelopathy. The parameters of SFEMG between different groups were compared. Results The mean jitter was (81.2± 25.9), (91.6 ± 32. 4) and (40. 9 ± 11.8) μ in ALS patients with finding of cervical spondylosis on MRI, ALS patients with normal MRI of cervical spine, and patients with cervical spondylosis respectively. M50 of the percentage of jitter >55 μs was 73%, 80% and 5% in the 3 groups respectively. M50 of the percentage of block was 10%, 20% and 0 in the 3 groups respectively. Fiber density was 2.9±0.5, 2.9±0.6 and 2. 4 ± 0. 6 in the 3 groups respectively. There was no significant difference in those parameters of SFEMG between the ALS with findings of cervical spondysosis and those with normal MRI. There was significant difference in those parameters of SFEMG between the patients with ALS (the mean jitter: (86. 3± 29. 6) μs; M50 of the percentage of jitter > 55 μs: 80% ; M50 of the percentage of block: 14% ; fiber density: 2. 9±0. 5) and those with cervical spondylosis (t = 14.49, Z = 8.96, 7. 68,t = 5. 83, all P = 0. 000). In 18 patients with ALS, conventional EMG studies showed active and chronic denervation in only 1 region at the first visit. SFEMG was performed in EDC which had normal Medical Research Council Scale and EMG. SFEMG showed increased fiber density in 16 patients, increased jitter in 13 patients and impulse block in 6 patients. Conclusion SFEMG shows significantly increased jitter and block in ALS whether there is MRI findings of cervical spondysosis or not, which can help to differentiate ALS from cervical spondysotic radiculopathy and myelopathy.