AIM: To evaluate the role of the meso-hippocampal dopaminergic systems in the generation of hippocampal P300-like potentials. METHODS: Changes of ventral hippocampal P300-like potentials were examined after lesions of the meso-hippocampal dopaminergic systems.The extracellular DA and DOPAC were collected by the push-pull method in the ventral and dorsal hippocampus and their contents were measured by high-performance liquid chromatography with electrochemical detection(HPLC-EC). RESULTS: The contents of DA and DOPAC in the ventral hippocampus were higher than those in the dorsal hippocampus in the normal animals. The peak latencies of P300-like potentials were prolonged and the contents of DA and DOPAC decreased in the ventral hippocampi after lesions of the meso-hippocampal dopaminergic systems. CONCLUSIONS: There exist meso-hippocampal dopaminergic systems in rats;The meso-hippocampal dopaminergic terminals is mainly located in the ventral hippocampal area in rats; The meso-hippocampal dopaminergic projections take part in the generation of the hippocampal P300-like potentials in rats.

AIM: To evaluate the effect of hexuemingmu tablet on retinal vein occlusion ( ROV) .
METHODS: Totally, 108 patients of 112 eyes were divided into two groups randomly. One group including 55 patients ( 55 eyes ) were treated by hexuemingmu tablet, the other group including 53 patients ( 57 eyes ) were treated by danhong injection. The visual acuity, fundus and FFA were evaluated before and after treatment.
RESULTS: The total effective rate in the group treated by hexuemingmu tablet was 98%, and that in control group was 82%. The hemorrhage was absorbed much more quickly than control group. Two group were different from each other in statistics (P<0. 05).
CONCLUSION: Hexuemingmu tablet can accelerate hemorrhage absorbing and improve visual acuity in RVO treatment.

Adult ; Arsenic Poisoning ; diagnosis ; drug therapy ; Humans ; Male ; Middle Aged ; Occupational Diseases ; chemically induced ; diagnosis ; drug therapy

Objective To study GFAP changes of astrocyte and expression of Endothelin-1 in molecular pathology in brainstem injury of human being and explore its forensic medical significance. Method 15 brain specimens of acute brainstem injury and 5 brain specimens of no brainstem injury in different time were selected and their microscopic morphological changes were observed with HE staining, and we study the pathological changes of GFAP of astrocyte and expression of Endothelin-1 in monoclonal antibody im-munohistochemistry SP method. Results The number of GFAP positive cells was increased, the bulk of the cell became bigger and expression of Endothelin-1 could be observed. Conclusion The above pathological changes might be a valuable base used for postmortem diagnosis of acute brainstem injury.

Aged, 80 and over ; Antigens, CD ; metabolism ; Antigens, Differentiation, Myelomonocytic ; metabolism ; Diagnosis, Differential ; Fasciitis ; pathology ; Fibroblasts ; pathology ; Fibrosarcoma ; metabolism ; pathology ; surgery ; Forearm ; Histiocytoma, Malignant Fibrous ; pathology ; Humans ; Male ; Myxosarcoma ; metabolism ; pathology ; surgery ; Soft Tissue Neoplasms ; metabolism ; pathology ; surgery ; Vimentin ; metabolism ; alpha 1-Antichymotrypsin ; metabolism

Qualitative and quantitative analyses of biological samples containing drugs, toxicants and endogenous substances play an important role in the researches of life sciences, as well as in new drug discovery and development. Biological samples are characterized by complex matrix, multiple endogenous interferences, significantly lower concentrations of measured analytes compared to endogenous components and small sampling volume. Consequently, it often requires bioanalysis methods with superior specificity, high sensitivity and good reproducibility. The two-dimensional liquid chromatography (2D-LC) technique, which allows for high peak capacity, significant reduced matrix effect and carryover of complex matrix samples and automated sample pre-treatment and analysis, has been the powerful solution to the separation and analysis of biological sample and widely applied to environment, food and pharmaceutical analysis. On the basis of introduction of the principle and equipments of 2D-LC, the application of this technique in the pharmacokinetics, toxicological and biological study was reviewed.

Objective To evaluate the effect of pneumatic ureteroscopic lithotripsy(URSL) for ureteral calculi.MethodsA total of 378 cases of ureteral calculi received URSL in our hospital by using F8/9.8 Wolf rigid ureteroscope and EMS Ⅱ pneumatic lithotripter(Switzerland).Among the cases,213 had calculi at the left side,158 at the right side,and 7 at the both sides;81 had the stones in the upper ureter,146 in the middle segment,and 158 in the lower part.Results In the cases,313 patients(320 sides) were cured after the first operation with a success rate of 82.8%(313/378).The success rates for the upper,middle,and lower ureteral calculi were 60.5%(49/81),84.2%(123/146),and 93.7%(148/158),respectively.The stones were removed completed in 1 to 8 weeks after the procedure.Fifteen patients were converted to an open surgery.In 3 patients,the URSL were stopped due to failure in placing the ureteroscope.The main postoperative complications in our series included perforation of the ureter(8 cases) and fever(13).Conclusions USRL is a safe,minimally invasive,and effective treatment.It should be the first choice for middle and lower ureteral calculi.

Objective To explorethe relationship between serum uric acid (SUA ) and transcutaneousoxygenpressure(TcPO2)inpatientswithT2DM.Methods 622hospitalizedpatients with T2DM were recruited and divided into high SUA (HUA) group and normal SUA (T2DM) group.The differences in TcPO2 (initial value ,stable value ,initial value‐stable value ,leg‐raising initial value and leg‐raising maximum value)were compared between two groups.The correlations between SUA and TcPO2 were evaluated. Results The levels of TcPO2 were lower in HUA group than in T2DM group [initial value:left (34.05 ± 13.17) vs (39.26 ± 15.82) ,right (34.71 ± 14.90) vs (40.15 ± 16.23);stable value:left (38.93 ± 12.23) vs(45.19 ± 13.40) ,right (39.98 ± 12.34) vs (45.90 ± 16.77) ,P<0.05]. Pearson correlation analysis showed that the SUA levels negatively related with the stable TcPO2 value of leftor right side ,and with the change between the initial value and the stable value of TcPO2 (P<0.05 or P<0.01). The multiple linear regression analysis showed that SUA level was the impact factorof the left TcPO2 stable value. Conclusion HUA may be one of the risk factors microcirculation disorder in T2DM patients.

Objective To analyze the correlative factors of prognosis of neonatal stress-induced ulcer bleeding.Methods Fifty-four cases of NICU admitted stress-induced ulcer bleeding newborns were divided into mild group and severe group according to bleeding endurance,transfusion requirement,and clinical turnover.Compared with gender,gestational age,birth weight,and score for neonatal acute physiology(SNAP) Ⅱ and score for neonatal acute physiologyⅡ perinatal extension(SNAP-PE) Ⅱ of two groups.Results Based on the output of SPSS statistical package,the SNAP Ⅱand SNAP-PE Ⅱ had a significant deviation between two subgroups((?~2=)15.207,10.311 all P