Objective:To establish a quantitative analysis of multi-components by single marker ( QAMS) method for the determi-nation of six components ( phillyrin, schizandrol, schisandra ester, deoxyschizandrin, schisandrin B and schisandrin C ) in Liuwei Wuling tablets,and verify the accuracy and feasibility of the method applied in the preparation. Methods:The relative correction factor ( f) of schisandrin B, schisandrin C, schisandra ester, schizandrol, phillyrin and deoxyschizandrin was detected by HPLC at the detec-tion wavelength of 230nm, and the contents of the components in Liuwei Wuling tablets were calculated to achieve the multi evaluation. Meanwhile, the contents of the six components were analyzed by an external standard method and the relative error between the calcula-tion of QAMS method ( Wf ) and the result of the external standard method ( Ws ) was calculated to verify the accuracy of QAMS. The reproducibility of the method was also investigated. Results:The QAMS method was established to determine the contents of 6 compo-nents in Liuwei Wuling tablets, and the components in 10 batches of Liuwei Wuling tablets were determined. The differences between the calculated values and the measured values were small ( RSD < 5%) . Conclusion:The QAMS method is simple, effective and ac-curate in determining the contents of 6 effective components in Liuwei Wuling tablets. The quality evaluation model of QAMS for Liuwei Wuling tablets is validated, which can be used for the quality control of Liuwei Wuling tablets and provide reference for the further study.

Objective:To simultaneous determine six flavonoids in Shuxuening injection by quantitative analysis of multi-compo-nents by single marker ( QAMS) and examine the durability and system suitability of the analysis. Methods:Shuxuening injection was the study object and rutin was used as the internal material, the relative correction factor ( f) between rutin and narcissus glycosides, quercetin-3-O-β-D-glucosyl (1-2)-α-L- rhamnoside, kaempferol-3-O-β-D-glucose group (1-2)-α-L-rhamnopyranoside, quercetin-3-O-[2-O-(1-O)-p-coumaric acid-β-D-glucose-yl]-α-L-rhamnoside, kaempferol-3-O-[2-O-(6-O)-on coumaroyl-β-D-glucose-based]-α-L-rhamnosides was respectively established. The result differences between the internal standard method and external standard method were compared, and the internal standard method was re-validated. Results: The contents of the six flavonoids in Shuxuening injection were calculated by the correction factors. Compared with those of the external standard method, the differences in the contents of the method were small( RSD<5%) . Conclusion:QAMS can be used as a simple and accurate quantitative quality evaluation meth-od for the determination of a variety of ingredients in Shuxuening injection, which provides reference for the subsequent study on quality standard.

Objective To observe the effects of Huoxue Xifeng Decoction (HXD) on endothelin-1 (ET-1) and nitric oxide (NO) levels in the blood and brain tissue of focal cerebral infarction (FCI) rats. Methods Forty male Wistar rats were randomized into 5 groups:sham-operation, FCI model and HXD-high, HXD-middle, HXD-low dose groups, with 8 rats in each group. The model of FCI rats was established by photo-chemistry method, and intragastric administration were continue 7 days before operation. HXD-high, HXD-middle, HXD-low dose groups were given HXD 20, 10, 5 g/(kg?d) respectively, and other groups were given the same volume distilled water. Blood and brain tissue samples were gotten 24 h after operation, and radio-immunity method was used to measure ET-1 level, spectrophotometric method was used to measure NO level. Results Compared with FCI model group, HXD decreased ET-1 level in plasma and brain tissue after infarction, and decreased NO level in the brain tissue, increased NO level in serum (P

AIM: Eye surface burn is easy to cause blindness. Amniotic membrane transplantation has become a hot spot of research for its special effects in recent years. This study observed the therapeutic outcome of fresh amniotic membrane transplantation for acute chemical or thermal burns at eye surface. METHODS: ①Eight eyes with acid burn in eight cases, ten eyes with alkali burn in ten cases, and seven eyes with thermal burn in six cases were selected from Yan'an University Affiliated Hospital between January 2002 and June 2007 including 23 males and 1 female aged 16-50 years. There were 18 eyes with preoperative vision

One hundred patients with type 2 diabetic neuropathy were divided into L-carnitine group and control group with 50 eases in each group. Data showed significant improvements in clinical symptoms scores, visual analogue scale and quantitative sense test after 16-week L-earnitine treatment (all P < 0.05). The study demonstrates that L-earnitine has affirmative effect on patients with diabetic neuropathy, especially in alleviating pain.

Objective To investigate the effect of psychological abuse in childhood on the personality of undergraduates with depression as well as its possible mechanism, and thus provide scientific evidence for the intervention of depression in undergraduates. Methods Particinants were 733 undergraduates from a university in Henan Province. They were surveyed with Center for Epidemiological Studies-Depression scale (CES-D), childhood experience of care and abuse questionnaire (CECA. Q) and Eysenck personality theory (EPQ). Results the correlation between maternal antipathy and introversion-extroversion of personality;psychological abuse in childhood had apparently positive correlation with the depression in undergraduates (r = 0. 257, r = 0. 304, r =0. 243, P＜ 0. 01) and that of childhood maternal neglect to psychoticism (β = 0.206, P＜ 0.01) were positive and significant. The standard regression coefficients of paternal neglect to introversion-extroversion (β= -0. 143, P＜0. 01)were negative and significant . The scores of childhood paternal antipathy and maternal antipathy explained that childhood paternal antipathy had indirect effect via adulthood neuroticism on the depression in undergraduates,furthermore, the indirect effect demonstrated 59.8% of the total effect. Conclusion This study suggests that the personality of undergraduates with depression is affected directly by psychological abuse in childhood, and adulthood personality may mediate the relation between childhood psychological abuse and adulthood depression.

Senecio scandens Buch.-Ham. , also called Jiuliming, is a plant of Senecio cruentus. It mainly contains alkaloids,phe-nolic acids,flavonoids,volatile oils,terpenoids and the other ingredients, and the main pharmacological effects include antibiosis,anti-inflammation,anti-virus, antitumor, antioxidation and free radical scavenger, toxicological effects and so on. The study progress in chemical constituents and pharmacological effects of Senecio scandens Buch.-Ham. was reviewed in the paper to provide available infor-mation for more research.

Objective:To construct an integrated medical care service mode in hemodialysis and explore the application effect of the concept of integrated medical service in hemodialysis patients.Methods:In 2016,100 patients who had regular hemodialysis(lasting for more than 3 months) were selected from July to Septembe ras the control group,while another100 patients who had the regular hemo dialysis (lasting for more than 3 months) and received the concept of integrated medical care service were selected from October to December as the observation group.The treatment compliance,the incidence of hemodialysis complications,and patient satisfaction of twogroups were compared.Results:Both the treatment compliance and patient satisfaction of the participants in the observation group were significantly higher than those in the control group (P ＜ 0.05),while the incidence of hemodialysis complications in the observation group was significantly lower than that in the control group (P ＜ 0.05).Conclusion:The implementation of integrated medical careservice concept can fully deepen the service concept of people-oriented and patient-centered,carry out humanized services,improve the treatment compliance of hemodialysis patients,reduce the complications,promote the cooperation of doctors and nurses,enhance the effect of health education,and improve the patient satisfaction.

Objective To analyze the clinical significance and correlation factors influencing the serum levels of CA125 and CA199 in patients with chronic nephropathy.Methods Clinical data of 649 hospitalized patients with chronic nephropathy from Jan.to Dec.in 2007 were retrospectively analyzed.The criteria of enrolment were: patients with chronic nephropathy aged below 70 years,tumor excluded by CT and ultrasound,and virus hepatitis and tuberculosis excluded by microbiological examinations.The serum levels of CA125,CA199,albumin,total cholesterol(TC),triglyceride(TG),24h urine protein and urine creatinine(Cr) were determined.The patients were divided into CA125/CA199 positive group and negative group according to the cutoff value of serum CA125 and CA199,and the clinical differences were analyzed by t or ?2 test.The correlation factors influencing the serum levels of CA125 and CA199 were analyzed by multiplicity.Results The ratio of female,nephrotic syndrome(NS),membranous nephropathy(MN) and hydrops of serous cavity were significantly higher in CA125/CA199 negative group than that in positive group(P

OBJECTIVE To find out fulminate epidemiological features of Stenotrophomonas maltophilia in ICU and the ways to prevent and treat this nosocomial infection.METHODS The case histories from 4 inpatients who developed S.maltophilia infection in the same ward from Feb 5 to 16,2006,were studied retrospectively to find out the reasons of its onset and its treatment based on the sputum culture results.RESULTS The fulminate S.maltophilia was found from the rail of the patient bed,the connection part of water container of 2 respiratory machines of exhalation valve assembly and the liquid of ultrasonic aeriation machine.CONCLUSIONS The infection is a localized one.The main reasons of the infection are unthorough disinfection of respiratory machine and the contamination of medical treatment environment.Whenever the infection is found in the ward,the thorough disinfection needs immediately,and no new patient admitted.