BACKGROUND: Sliding cupping has been used for treating cervical spondylosis, with the effects of dredging the meridian system, activating qi and removing blood stasis.OBJECTIVE: To probe into symptomatic improvement of cervical spondylosis with sliding cupping and the relationship between curative effect and course of disease as well as age.DESIGN: Self controlled observation.SETTING: Department of Physiotherapy, General Hospital of Air Force of Chinese PLA.PARTICIPANTS: Totally 100 out-patients with cervical spondylosis receiving treatment in the Department of Physiotherapy, General Hospital of Air Force of Chinese PLA were involved from December 2000 to December 2003. Among them, 39 cases were male and 61 cases were female; 9 cases were under 30 years old, 38 cases were 31 to 40 years old, 42 cases were 41 to 50 years old and 11 cases were above 50 years old. There were 36 cases with less-than-one-year course of disease, 49 with 1 to 5-year course and 15 with more-than-5-year course. Fifty-one cases were cervical type; 25 cases vertebral artery type; 24 cases nerve root type. X-ray examination revealed that there were 69 cases whose cervical vertebral physiological curvature became straight or reverse, or with certain degree of degenerative change. Vertebroarterial blood supply insufficiency was detected in 23 cases by rheoencephalogram.INTERVENTIONS: The patients were asked to be in prone sitting position,their nape and shoulder were fully exposed, and the negative pressure cupping was applied to patient' s nape and shoulder until petechiae appeared on the skin. The treatment was given once or twice a week. After one-month treatment, the curative effect was calculated according to subjective symptoms, signs, and the results of X-ray and rheoencephalogram examination before and after treatment. Symptom disappearance: pain was 0 grade; the cervical vertebral physiological curvature restored by 10°- 15° and rheoencephalogram showed that the contraction peak velocity of the vertebroarterial blood supply became normal. Marked improvement: the pain reduced by more than 2 grade, the cervical vertebral physiological curvature restored by 6°9° and rheoencephalogram showed that the vertebroarterial blood supply restored to 80% of the normal range. Improvement: the pain reduced by 1 grade, the cervical vertebral physiological curvature restored by 3°- 5° and rheoencephalogram showed that the vertebroarterial blood supply restored to 40% - 80% of the normal range. Inefficacy: the pain did not reduce, the cervical vertebral physiological curvature restored by less than 3° and rheoencephalogram showed that the vertebroarterial blood supply became less than 40%.RESULTS: According to the intention-to-treat analysis, 100 patients entered the result analysis. For symptom disappearance, marked improvement,improvement, inefficacy there were 27, 17, 5 and 2 cases respectively in cervical type; 8, 10,4 and 3 cases respectively in the vertebral artery type;4,9,7 and 4 cases respectively in the nerve root type. There were 19, 12, 3 and 2 cases respectively with less-than-one-year course of disease; 18, 19, 7 and 5 cases respectively with 1 to 5-year course; 2, 5, 6 and 2 cases respectively with more-than-5-year course. And there were 5, 3 and 1 cases respectively with age ＜ 30 years; 18, 13, 4 and 3 cases respectively with age of 31 to 40 years; 15, 18, 5 and 4 cases respectively with age of 41 to 50years; 1, 2, 6 and 2 cases respectively with age ＞ 50 years.CONCLUSION: The effect of sliding cupping was good for patients with cervical spondylosis of vertical type, next of vertebroarterial type and bad of nerve root type. The shorter the course of disease is, the younger the patient is, and the better the curative effect is.

Objective To study the adiponectin mRNA expression in omental adipose tissue of type 2 diabetes. Methods RT-PCR method was used to examine the adiponectin mRNA expression. Fasting levels of BG,TG,TC,HDL-C,LDL-C of all the subjects were checked and their blood pressure,height,weight,waist circumference,hip circumference were measured to calculate BMI,WHR. Results The adiponectin mRNA expression in omental adipose tissue was decreased in diabetes group versus non-diabetes group (P<0.05),and negatively correlated with WC,TG,FBG,WHR(P<0.05). Conclusions Type 2 diabetic patients show lower expression of adiponectin mRNA in omental adipose tissue than that of non-diabetic control.WC,TG,FBG, WHR and course of disease are correlated with adiponectin mRNA expression and insulin resistance

Objective:To investigate the effect of Aspirin on the proliferation and NOS expression in astrocytoma cell line,and probe into ins mechanism.Methods:The effect of Aspirin on the growth of astrocytoma cells evaluated by MTT assay;NOS protein levels were determined by immunohistochemistry,NO and CEA concentration in the medium were determined by Griess assay and lepton catch immuning method respectively.Results:Aspirin can inhibit the growth of astrocytoma cells,induce the expression of iNOS,increase the concentration of NO in the medium. The effect of these were in a concentration dependent pattern. Moreover,Aspirin can reduce the concentration of CEA in the medium.Conclusion:Aspirin inhibit the growth of astrocytoma cell line.UP regulated iNOS expression resulting a increase of NO concentration are ascribed to mechanism of antrproliferation activity of Aspirin. CEA is a good indicator in monitoring curative effect of astrocytoma.

Objective To evaluate the clinical effect and application value of internal fixation of Magerl combined with Brooks technique for atlantoaxial instability. Methods A total of 18 patients with atlantoaxial instability, reducible atlantoaxial dislocation with reduction after traction and irreducible atlantoaxial dislocation with traction reduction after anterior laxation were treated with internal fixation of Magerl combined with Brooks technique using autologous bone grafts. Results The patients were followed up for an average 18 months (6-36 months). Screw was probably cut into the vertebral artery in one case. The guide needle was cut into the pharynx due to incorrectly penetrating the C 1 anterior arch of atlas for 3.5 cm in one case but no early complications such as spinal cord injury and local infection occurred. The spinal cord function improvement was marked in 3 cases, good in 9, mild in 4 but unchanged in 2. No case got worse. There were no loosening or break of the screw and cable. Conclusions Combined fixation of Magerl and Brooks techniques reaches three-point fixation and improves the reduction effect. It is necessary to use the combined fixation with best biomechanical stability for atlantoaxial dislocation patient with reduction or near reduction if the structure of the posterior arch of C 1,2 is intact.

0.05). The level of serum IL-8 and expression of TNF ? in the colonic mucosa decreased at the 7th day ( P

Humans ; Liver Diseases ; diagnosis ; pathology

Objective To summarize the experience of preventing infection in atlanto-axial operations via transoropharyngeal approach. Methods The methods and experiences of prevention of infection in 80 cases of atlanto-axial dislocation operated on via transoropharyngeal approach were retrospectively summarized and analyzed. Result There was no infection in all the 80 cases. Conclusion As long as preventive measures were perfectly taken, the post-operative infection could be effectively prevented and the transoropharyngeal operation would be safe.

Objective To evaluate the clinical results of subtotal vertebrectomy of the axis for spinal canal decompression by transoral anterior approach in the treatment of atlanto-axial dislocation with spinal cord compression. Method fifteen cases of atlanto-axial dislocation with spinal cord compression caused by congenital deformity or trauma were subjected to subtotal vertebrectomy of the axis via transoral anterior approach . Results After an average follow-up of 20 months, curative effect was evaluated according to Symon and Lavender. Vertebral canal vector diameters in MRI were measured. The total clinical effective rate was 100%, and the remarkable effective rate reached 60%. The average improvement rate of vertebral canal decompression was 79.8%. No sign of spinal cord and arteries injury and infection were observed. Conclusion Subtotal vertebrectomy of the axis via transoral anterior approach is proved to be feasible to treat cervical spinal cord compression on C 2 vertebra level.

Background The filtration surgery is the main method of treating glaucoma,which usually fails due to postoperative scarring.The study about application of anti-scarring agents in filtration surgery is a hotspot.Objective This study was to investigate whether topical administration of hydroxycamptothecin (HCPT) could be used to prevent postoperative scarring in after experimental glaucoma filtration surgery,and explore its optimal dose.Metbods Trabeculectomy was performed on the right eyes of 40 New Zealand white rabbits to establish the trabeculectomy animal models.The rabbits were then randomized into normal saline solution group,0.3 g/L mitomycin C(MMC) group,0.3 g/L HCPT group and 1.0 g/L HCPT group based on the intraoperative topical drugs using randomized number table method.The different drugs above-mentioned were put beneath the conjunctival flap and scleral flap for 5 minutes during the surgery.The intraocular pressure (IOP) was measured before and day 1,4,7,14,21 and 28 after the surgery with Icare tonometer,and the filtering area and height were measured under the slit lamp microscope to assess the efficacy of various drugs.The adverse effects were evaluated by examining the responses of the ocular anterior segment and retinal change.The specimens at operative zone were obtained in 7,14 and 28 days after surgery with the size 5 mm×5 mm for the hematoxylin-eosin staining and Masson trichrome staining to estimate the anti-fibrosis effect of various drugs,and to evaluate the survival time of functional bleb.Kaplan-Meier analysis was used to compare the survival time of functional bleb of different groups.The use and care of the animals complied with the Regulation for the Administration of Affair Concerning Experimental Animals by State Science and Technology Committee.Results The IOP was significantly different in the rabbits from different groups among various time points (Fgroup =20.79,P =0.00 ; Ftime =85.34,P =0.00 ; Fiion =2.13,P =0.01).From 1 day through 28 days after operation,the IOPs in MMC group and 1.0 g/L HCPT group were significantly lower than those before operation (all at P＜0.05).The survival time of functional bleb of different groups was (11.3 ±2.8),(19.5 ±2.4),(13.3 ±2.2) and (20.2 ± 4.5) days,respectively,showing a significant difference (log rank =11.92,P ＜ 0.01),with a considerable prolong in the 1.0 g/L HCPT group.No significant change was found in the bleb area and height among the four groups within 7 days after operation,but postoperative 7,14,28 days,the area and height values of bleb were significantly smaller in the normal saline solution group and 0.3 g/L HCPT group compared with the MMC group and 1.0 g/L HCPT group (all at P ＜ 0.05).Histopathological examination showed loosen subconjunctival tissue,less inflammatory cells and weaker collagenous fibrillary staining in the MMC group and 1.0 g/L HCPT group in comparison with the normal saline solution group and 0.3 g/L HCPT group.Conclusions The topical administration of 1.0 g/L HCPT inhibit the inflammatory response and collagen fibrosis and therefore prolong the survival time of functional bleb after glaucomatous filtering surgery.

Purpose To observe the action time of parthenolide (PTN) as selective nuclear factor-κB(NF-κB) inhibitor in heart of rats.Methods Forty SD rats were randomly divided into control(CON) group,PTN group,dimethyl sulfoxide(DMSO) group,lipopolysaccharide(LPS) group and PTN+LPS group.The first day:PTN group,DMSO group and PTN+LPS group rats were respectively injected interapertoneally PTN(500 μg/kg) and DMSO; other groups rats were injected interapertoneally equivalent saline.The second day(in equivalent to 24 h after injecting PTN):in LPS group and PTN+LPS group LPS(12.5 mg/kg) was administered subcutaneously; other group rats were injected subcutaneously equivalent saline.Every heart was obtained in equivalentce to 1 h after injecting LPS to measure the expression of NF-κB p50 in nucleus,inhibitory kappaB(IκBα) and phosphorylation inhibitory kappaB(p-IκBα) in cytoplasm of cardiac myocytes through Western blotting.Results Compared with CON group,the expression of NF-κB p50,IκBα and p-IκBα had no difference in PTN and DMSO group.And in LPS group and PTN + LPS group,the expression of NF-κB p50 up-regulated significantly(P＜0.05),IκBα down-regulated(P＜0.05) and p-IκBα up-regulated significantly(P＜0.05).Conclusion LPS can activate NF-κB in 1 h and PTN in rats after 24 h has few effect.