10 points or if the progressing osteoarthritis was proved by the T nnis grade.And the remaining patients would join in the satisfied group.The differences of preoperative clinical and radiographic factors between unsatisfied group and satisfied group were identified.[Result]Ten hips in the unsatisfied group had significantly more WOMAC pain score(t=3.969,P

Objective To investigate the medial-lateral tilting of patella in two different coordinate systems before and after total knee arthroplasty (TKA). Methods Six fresh-frozen left low limbs from cadaver were tested using a modified Oxford Rig. Anatomically based multiple loading of the quadriceps mechanism was applied(Vastus medialis 24.5 N, Vastus intermedius/rectus femoris 29.4 N, Vastus lateralis 19.6 N). In a three dimensional coordinate system, patellar tilting was tracked using a custom-made Laser tracking device. The femoral trochlea was used as the other coodinate system in skyline view of patellar-femoral joint. Total knee arthroplasty was performed using PFC? posterior cruciate ligament substituting knee system. The tilting angles of patellar were measured before and after TKA. Results In three dimensional coordinate system, when knee flexed 30?,60?,90?,120?,150?, the patella titled :-3.10??2.25?,2.50??1.07?,5.46??2.60?,6.32??2.16?,-2.10??1.90? respectively before total knee arthroplasty was undertaken. After total knee arthroplasty, the patella tilted 3.24??0.35?,2.30??1.39?,1.65??1.17?,1.29??1.03?,-0.86??1.42? respectively. When femoral trochlea was taken as coordinate, the patella tilted 0??0?,0??0?,0??0?,0.24??0.35?,-3.21??1.80?; 0??0?,0??0?,0??0?,0??0?,-1.12??1.20? respectively both before and after TKA (Lateral patella tilting was defined as positive and medial patella tilting, negative). Conclusion Even the relationship between patella and femoral trochlea remains normal, the mode and magnitude of patellar tilting changes significantly after TKA. In early flexion of knee joint, patella tilts medially before TKA while it tilts laterally after TKA. After TKA, patella tilts less laterally than it does before TKA when a knee flexes from 60 to 120 degrees.

Breast reconstruction after radical mastectomy significantly improves the patient' s quality of life and has become an important part of comprehensive treatments. However, there are a number of reconstruction problems. The choice of breast reconstruction after breast cancer should be considered in general. Immediate reconstruction is not superior to breast-conserving surgery, while immediate reconstruction is superior to delayed reconstruction, and choices vary between implant reconstruction and autologous reconstruction. In short, breast reconstruction should be based on the radical principle with balance of beauty.

ObjectiveTo compare therapeutic effects of the bone-guided tissue regeneration in combination with a composite bovine-derived xenograft versus flap surgery only on intra-bony defects in elderly patients with periodontal disease. MethodsThirty elderly patients with periodontal disease were randomly divided into two groups. One group was treated by the bone-guided tissue regeneration in combination with a composite bovine-derived xenograft (experimental group). The other group was treated by flap surgery only (control group). Probing depth (PD)and clinical attachment level (AL) were determined before surgery and six months after treatment in two groups. The change of bone amount was also determined before surgery and six months after treatment through computer-assisted densitometric image analysis(CADIA). ResultsThe changes of PD and CADIA were (3.8+1.7)mm, (20. 3+11.1)g/mm2 in experimental group and were (2.5+1.1)mm, (9.4+8. 6)g/mm2 in control group. The differences between two groups were significant (P.＜0. 05). The change of AL was (3.5+ 1.6)mm in experimental group, compared with control group(2. 3 1.7)mm, which showed more obvious regeneration of alveolar bone (P＜ 0. 01). ConclusionsGuided tissue regeneration in combination with a composite bovine-derived xenograft appears to be more effective than flap surgery only for intra-bony defects in elderly patients with periodontal disease.

Objective To ascertain serum inflammatory markers could be modified following treatment of periodontal disease in elderly patients. Methods The probing depth (PD), clinical attachment level (AL), C-reactive protein (CRP), interleukin-6 (IL-6) were determined. And then fifty-two elderly periodontitis patients underwent a standard phase of non-surgical periodontitis treatment (consisting of oral hygiene instructions and subgingival scaling and root planning). After three and six months, PD, AL, CRP and IL-6 were determined again and compared to the baseline. Results Six months after treatment, significant reductions in PD [(5.9±1.1) mm vs. (6.8±1.0) mm, P＜0.05], AL [(1.3±0.9) mm vs.(8.4±1.1) mm, P＜0.05], CRP [(1.5±0.2) mg/L vs. (2.0±0.3) mg/L, P＜0.01] and IL-6 [(1.6±0.5) ng/L vs. (1.9±0.4) ng/L, P＜0.05] were observed. Conclusions Treatment of chronic periodontitis can decrease the levels of serum inflammatory markers in elderly patients.

Objective To investigate the coupling of tibial rotation and extension-flexion motion of knee joint both before and after total knee arthroplasty. Methods Six fresh-frozen left lower limbs from cadavers were tested using a modified Oxford motor rig for the knee joint. Anatomically based multiple loadings of the quadriceps mechanism were applied (Vastus medialis: 24.5 N, Vastus intermedius/rectus femoris: 29.4 N, Vastus lateralis: 19.6 N). The tibial rotation was tracked using a custom-made laser tracking device while the tibial-femoral flexion was measured with goniometer. Total knee arthroplasty (TKA) was performed using ?PFC-PS posterior cruciate ligament substituting knee system. Results Before TKA, when the knee flexed 10?, 20?, 30?, 60?, 90?, 120? and 150?, tibia rotated 5.1??2.3?, 9.3??3.3?, 10.2??4.8?,11.7??3.5?, 13.5??8.4?, 22.0??8.6? and 29.6??9.3? respectively. After TKA, tibia rotated -2.2??1.7?, -2.5??1.5?, -2.0??1.3?, 1.7??2.5?, 3.9??2.4?, 13.5??6.1? and 18.6??4.7? respectively (Tibial rotation was defined as 0? in knee extension; internal rotation as positive and external rotation as negative). Conclusion The tibial internal rotation is coincident with tibial-femoral flexion, its magnitude of tibial rotation varies in different degrees of knee flexion. The tibial internal rotatation is significant in early knee flexion(0? to 30?), then enters into a platform stage on knee bending from 30? to 90?, Once the knee bends over 90?, the tibia rotates internally markedly again, maximum internal rotation is achieved in fully flexion, probably due to relaxation of soft tissue. After TKA, in early flexion (0? to 30?) the external rotation occurs abnormally, but later on 30? to 150?, it rotates again intenally as normal.

Objective To identify a unique protein as a novel genetic marker for rapid molecular typing of Mycobacteriutn tuberculosis Beijing genotype strains by comparing the proteome of Beijing genotype strains with non-Beijing strains.Methods Fifty-six clinical isolates of Mycobacterium tuber- culosis were analyzed by spoligotyping to determine genotypes.The two-dimensional electrophoresis (2 DE)was used to compare the global protein patterns between Beijing genotype strains and non Bei jing strains.Differential expressed proteins were measured by matrix assisted laser desorption ioniza tion lime of flight mass spectrometry(MALDI-TOF-MS).The data obtained from peptide mass fingerprinting were compared in protein database.The genes encoding differential expressed proteins and their upstream were sequenced.Results Forty nine of the 56 isolates were Beijing genotype strains and 7 isolates were non-Beijing strains.A unique protein Rv0927c was identified,which is absent in Beijing genotype strains compared with 7 non Beijing strains and H37Rv.There were two characteristic mutations in Beijing genotype strains,a deletion of AGC at nucleotide position 421 of Rv0927c and a 127 G→A muta- tion in the upstream of Rv0927c.but not in non Beijing strains and H37Rv.Conclusion Characteris tic mutations of Rv0927c in Beijing genotype strains can be used as a novel genetic marker for rapid molecular typing of Mycobacteriuln tuberculosis Beijing genotype strains and non Beijing strains.

Objective To observe the effect of two different periodontal therapies on serum levels of C-reactive protein (CRP) and tumor necrosis factor-alpha(TNF-α)in elderly patients with coronary heart disease.Methods Totally 42 elderly patients with severe periodontitis were randomized into two groups:periodontal initial therapy and periodontal flap surgery group.The percentage of bleeding on probing (BOP),probing depth (PD) and serum levels of CRP and TNF-α were recorded at baseline and 12 weeks after treatment.Results The percentage of BOP,PD and serum levels of CRP and TNF-α in all patients were significantly reduced at 12 weeks after treatment as compared with baseline (all P＜0.05).Serum levels of CRP and TNF-α were lower in periodontal flap surgery than in periodontal initial therapy at 12 weeks after treatment [(3.4±9.1) μg/L vs.(2.6 ±7.9)μg/L,(4.4±1.8)mg/L vs.(3.5±2.1)mg/L,both P＜0.05].Conclusions Compared with periodontal initial therapy,periodontal flap surgery maybe better to reduce the levels of serum inflammatory markers.

Objective To investigate the effect of preconditioning with sodium butyrate on myocardial I/R injury. Methods Anesthetized rats were treated with sodium butyrate (100 or 300 mg/kg, i.p.) 30 mins before ischemia, and then subjected to ischemia for 30 min followed by reperfusion for 4 h. LDH, CK, TNF-α, IL-6, HMGB1, infarct size, MDA and SOD activity were measured. The infracted size was tested by TTC assay; The expression of HMGB1 was observed by western blot. Results After 4 h reperfusion, pretreatment of sodium butyrate (300 mg/kg) could significantly reduce the infarct size and the levels of LDH and CK (P<0.05)comparing to the control group; inhibit the increase of the MDA level and the decrease of the SOD level(P<0.05), also inhibit the expression of TNF-α, IL-6 and HMGB1 (all P < 0.05) induced by I/R. Conclusion Preconditioning of sodium butyrate can attenuate myocardial I/R injury by inhibiting inflammation response.

Ten pharmacophore models of beta-tubulin inhibitors were established from the training set of seventeen beta-tubulin inhibitors (two categories) with comformer analysis by using the Catalyst software. The optimal pharmacophore model with two hydrophobic units and two hydrogen bond acceptor units were confirmed (RMS = 0.43, Correl = 0.98, Weight = 2.06, Config = 15.97). This pharmacophore model is able to predict the activity of known beta-tubulin inhibitors and can be further used to identify structurally diverse compounds with higher activity.