The authors discussed the development of the assistive devices and service in China and overseas by analyzing the calssification, function, demand and difference between China and other countries.

AIM:To observe the effects of advanced glycation end-products (AGEs) on thioltransferase (TTase) expression and activity in human lens epithelial cells.
METHODS: Human lens epithelial cells B3 ( HLE B3 ) were treated with 1. 5mg/mL AGEs - BSA as the experimental groups cultured by fetal bovine serum of volume fraction 10% dulbecco modified eagle medium ( DMEM) and bovine serum albumin ( BSA) was added at the same concentrations as the negative control. The level of reactive oxygen species ( ROS ) was evaluated. Cells were collected at 0, 1, 2, 3, 4d and total RNA or protein was extracted. TTase mRNA levels were detected by qRT-RCR. TTase expression was detected by Western blot and its activity was measured.
RESULTS: Compared with the control group, AGEs-BSA up-regulated the expression of ROS (P<0. 01), ROS content increased in a time-dependent manner. BSA had no effects on ROS expression. The expression of TTase increased after treatment with AGEs-BSA for 1d, peaked at 2d (nearly 5. 06-fold increase, P<0. 01), then decreased gradually. No change was observed between BSA and control group (P>0. 05). Similarly, TTase activity peaked at 3d (nearly 2. 01-fold increase, P<0. 01). Western blot test found that TTase protein expression was increased
gradually, starting from the 3d TTase expression was reflected that there was statistically significant difference compared with control group (P<0. 05).
CONCLUSION:AGEs-BSA significantly increases the production of ROS in human lens epithelial cells, and it then induces the oxidative stress which may promote the expression of TTase and enhances the activity of TTase.

Objective To evaluate the prognosis of acute stroke by APACHEⅢ scoring system on and analyze some correlating factors.Methods 136 patients with acute stroke received APACHEⅢ scoring every day in the first week of their admission of hospital.All patients were divided into different groups respectively: death group and alive group according to the outcome of disease,midline group and non-midline group according to the location of illness,large-focus group and small-focus group in terms of volume of cerebral hemorrhage or cerebral infarction.Results APACHEⅢ scores of death group,midline group and large-focus group show significant differences from those of the other groups(alive group,non-midline group and small-focus group).The former is much higher than that of the latter.The highest accuracy can be observed when predicted mortality is 0.4.Conclusion The APACHEⅢ scoring system is a valuable reference for evaluating the severity and prognosis of acute stroke.

The authors discussed the relationship between assistive devices and persons with disability by giving examples such as physical and living conditions of persons with disability, rehabilitation process and conditions, etc., to show the importance of assistive devices to persons with disability.

Objective To investigate the therapeutic value of high-resolution microendoscopy for early esophageal cancer.Methods A total of 60 patients,who underwent esophageal endoscopy,were included in this study.The therapeutic value of high-resolution microendoscopy was investigated with reference to pathological results as golden standard.Results Diagnostic sensitivity,specificity,positive predictive value,negative predictive value and accuracy of microendoscopy for high grade dysplasia/early cancer were 81.22％,94.12％,81.82％,94.12％,91.11％,respectively.Diagnosis of severe dysplasia/carcinoma with high-resolution microendoscopy was highly consistent with that of pathology (K =0.760).Conclusion The high-resolution microendoscopy is of high diagnostic value for early esophagus cancer.

Objective To explore the effects of Tianqijiangtang capsule combined with Metformin on the levels of IL‐6 ,TNF‐α and C‐RP protein in patients with T2DM. Methods 80 T2DM were randomized into control group (treatment with Metformin ,n= 40) and treatment group (treatment with Tianqijiangtang in combination with Metformin ,n=40). The changes of levels of IL‐6 ,TNF‐α and C‐RP were observed before and after the treatment. Results Compared to the pretreatment in the same group and after treatment in control group ,IL‐6 ,TNF‐αand C‐RP of treatment group were reduced significantly [(6.79 ± 0.60) ,(7.15 ± 0.62) vs (5.08 ± 0.43)pg/ml;(8.94 ± 0.55) ,(8.61 ± 0.66) vs (5.18 ± 0.45) pg/ml;(6.79 ± 0.60) ,(6.58 ± 0.59) vs (5.56 ± 0.30) mg/L ,P<0.01]. Conclusion Tianqijiangtang capsule combined with Metformin can decrease the levels of IL‐6 ,TNF‐αand C‐RP in T2DM patients.

This paper discussed AIDS epidemic sityation and it's tendency, AIDS harmful-ness to human beings health and social economic development. The dangerous factors of AIDS epidemic in china is pointed out. prsvent and treatment AIDS is urgency.

Objective To study the method for preparing the polyamine cotton fiber loaded with Cu2+ and to test the antibacterial effect of the cotton fiber. Methods The natural cotton fiber was treated through basification, epoxygenation, alkene amination and Cu2+ adsorption. 8 kinds of microorganisms were used for the bacteriostasis test. Results The modified fibers showed a good antimicrobial effect to gram-negative bacteria and the bacteriostasis value achieved 6. The minimal sterilization concentrations of the Cu2+-diethylene triamine cotton fiber, Cu2+-triethylene tetramine cotton fiber and Cu2+-tetraethylene pentamine cotton fiber were 12 mg/ml, 14 mg/ml and 10 mg/ml respectively. The antibacterial process of the cotton fiber included two parts, adsorption and sterilization. The acting site was on the cell membrane. A positive relationship was seen between the concentration of antibacterial cotton fiber and the dissolved rate of bacteria. Conclusion The metal ion fiber shows an excellent performance of broad-spectrum antibacterial effect, especially to gram-negative bacteria and may be the "contact killing" is the acting manner of the antibacterial cotton fiber.