An enzyme immunoassay (EIA) for serum thyrotropin receptor antibodies (TRAb) was described. Polystyrene ball (3.2 mm in diameter) coated with solubilized guinea pig fat cell membranes (SGEM) was incubated with human serum and subsequently with horseradish peroxidase (HRP) conjugated staphylococcus protein A in a total volume of 50 ?I. The HRP activity was measured by spectrophotometer in a micro-cell with 3,3'5, 5'-tetramethylbenzydine (TMB) as substrate and 38 of 40 (95%) untreated Graves'patients (0.28?0.06, X?SD) and 15of 15 (100%) relapsed patients (0.25?0.06) had OD values at least 2 SD above normal subjects (0.09 ? 0.03), P

Objective To analyze the correlation of prognosis and early gastrointestinal nutritional intervention of critically ill ICU patients.Methods 378 cases of critically ill ICU patients from June 2010 to December 2011 were selected.260 cases of critically ill ICU patients were set as the treatment group,who were given appropriate care and nutritional support (early nutritional intervention was implemented within 24 hours) on the basis of conventional drug therapy.The remaining 118 cases were named as the control group,who received conventional therapy and conventional care.The nutritional items and their correlation were analyzed.Results After treatment,the items such as serum pre-albumin,protein intake,hemoglobin,and cholesterol were significantly different from those before treatment in both groups,and the improvement degree was higher in the treatment group than those in the control group.The cholesterol level was correlated with protein intake.Conclusions The application of early gastrointestinal nutritional intervention in critically ill ICU patients can effectively improve the hemoglobin level,reduce mortality and the incidence rate of complications,so it is necessary to be applied in clinic.

Contemporary human world has been afflicted with predicament and challenge,which Can be fundamentally attributed to self-cognition of humans.In other words,humans have lapsed into spirit crisis.The crux of this matter lies in the fact that a unidimensionally driven school,mostly motivated and dominated by instrumental rationality,will only produce a people as base as itself.Furthermore,people without multi-values are considerably likely to lose spirit felicity.To confront and solve this social problem,we ale obliged to attach more importance to the educational realistic significance of guiding people to spirit felicity of life.We need to know education being what individual spirit grow well to maturity with excellent individuality.We need to,by means of education,direct life value of people to the pursuit of happiness and the abatement of affliction,and form noble spirit of life with the increase of aesthetic ability.In order to strive to achieve education's intrinsic significance in self-contained and self-sufficient life value,we must largely focus on self-achievement of teachers.Only with such education Call we be guided to spirit Felicity of life.

Objective:To evaluate the role of the retinoic acid receptor ?(RAR?),retinoic acid receptor ?(RAR?) in the devel-opment and prognosis of the endometrial carcinoma by detecting the expression of these receptors. Methods:48 cases of paraffin-embedded tissues were selected, including 42 cases of adenocarcinoma and 6 cases of adeno-squamous carcinoma of endometrium. Immunohistochemistry was used to detect the level of RAR? and RAR?. The relation between the expression of these receptors and the clinical pathological parameters was evaluated. Results:The positive expression ratios of the RAR? and RAR? were 47.92%(23/48)and 25.00%(12/48),respectively. The level of RAR? was significantly higher than that of RAR?(P0.05). The level of the RAR? decreased with the increasing of the malignancy extent of the endome-trial adenocarcinoma(P0.05)respectively. Conclusion:There is close relation between the ex-pression of the RAR? and the development of the endometrial carcinoma. The defect of the RAR? and the high expression of the RAR? indicates a bad prognosis of the endometrial carcinoma. Detection of these two is benefited receptors together for us to estimate the prognosis of the carcinoma.

Objective:To give clinical evaluation to the caesarean section caused by social factor and get information for raising the quality of obstetric. Methods:The retrospective analysis was practiced on the obstetric quality index of the caesarean section caused by social factor and non-social factors in our institution from 2003.01 to 2003.12. The caesarean birth rate, social factor causing caesarean birth rate and forceps birth rate in 1994, 1999 and 2003 in our institution were compared. Results:The caesarean birth rate was 54.53% and the social factor causing caesarean birth rate was16.08% in 2003. The operation duration, postoperative fever days and bowel movement recovery duration of social factor group were longer than those of non-social factor group significantly(P0.05). Comparing 2003 with 1994 and 1999, the caesarean birth rate, social factor causing caesarean birth rate and forceps birth rate were different significantly(P

In order to develop quality education which focuses on fostering students' innovative consciousness and practical ability, we should make clear the significance of innovative quality education, and improve the quality of innovation through education of a new meaning on cultural and psychological course, guide of value, and self-construction; we should make sure of its present value to our society and also its significance on quality education, so as to improve our understanding of the importance and necessity of innovation education, and carry out an overall quality education which chiefly works for the training of students' innovative consciousness and practical ability.

Accreditation of laboratory is introduced concerning its origin,development,function and application.This paper emphasized its importance in the standardization and enhancement of laboratory management.

ObjectiveTo observe the effects of treating cerebral hemorrhage by the brain-located mild hypothermia therapy. Methods40 patients were divided in to two groups randomly, treatment group (20cases) and control group (20cases). Drugs were used in both groups, and the located mild hypothermia therapy was just applied in treatment group. After 7 days ,the volume of hydrocephalus and the Nerve Function Deficiency(NFD) score of hemiplegic limbs were compared.ResultsThe volume of hydrocephalus of treatment group was significantly smaller than that of control group(P<0.01). There were significant differences on the neurological impairment score between two groups (P<0.01).Conclusions The brain-located mild hypothermia therapy can reduced hydrocephalus in patients with cerebral hemorrhage.

Objective To develop an ion chromatography (IC) for simultaneous determination of chlorite,chlorate and bromate in drinking water. Methods The IC separation was carried out with the IonPAC AS23 column by using 4.5 mmol/L Na2CO3-0.8 mmol/L NaHCO3 at the flow rate of 1.0 ml/min. The injection volume was 250 ?l,the temperature of conductivity detector cell and column were 30 ℃ and 35 ℃,respectively. The pressure upon the bottle of mobile phase was 40 kPa. The current of auto-regenerating suppressor was 25 mA. Results The results showed that in the range of 0 to 1 000 mg/L, the calibration equation for chlorite was y=0.009 6+1.600 0 x (r=0.999 3),for bromate was y=0.003 2+3.184 7 x (r=0.999 9),for chlorate was y= 0.001 8+1.788 9 x(r=0.999 9).The detection limit of chlorite,chlorate and bromate were all 5 ?g/ L,the recovery rates were 89.0% -108.0% and the relative standard deviations (RSD) were 0.21%-2.70%. Conclusion The method is simple,fast,accurate, sensitive,little interference and is applicable to the simultaneous determination of chlorite,chlorate and bromate in drinking water.

Objective To explore the reason and influence of communication difficulties between oncology nurses and patients or families of patients,and to obtain some relevant advice about communication difficulties.Methods This study used focus group to interview 24 oncology nurses who were selected from the First Affiliated Hospital of Chongqing Medical University by homogeneous sampling method,and were randomly divided into three groups (8 in each group).The data were processed and analyzed by content analysis.Results The reasons why oncology nurses had communication difficulties with patients and their families were as follows:putting forward negative problems about their disease,ineffective communication and conflicts with patients,families.The communication difficulties showed negative effect on oncology nurses.Oncology nurses thought that the main reason of communication difficulties lied in the deficiency of institution and propounded the suggestion to resolve it.Conclusions oncology nurses were tend to have difficulty in communicating with patients and their families in the long-term work,especially in the period of crisis:early diagnosis of tumor stage,relapse,advanced cancer and some special dates (birthday,parties etc.).Therefore,oncology nurses,patients and their families need to be supported.To solve the difficulties of communication could reduce the negative emotions of patients and their family,such as anxiety,frustration,helplessness,so as to improve the quality of care and life of patients.