Objective:To evaluate the safety and efficacy of high-intensity focused ultrasound (HIFU) ablation for the VX2 liver tumor near the hepatic main blood vessels of rabbits by using dosimetry and magnetic resonance imaging (MRI) and by analyzing path-ological changes and survival. Methods:Rabbits with VX2 liver tumor adjacent to the abdominal aorta were divided into the HIFU (n=32) and control groups (n=20). MRI-guided HIFU was employed for the ablation of the liver tumor in the HIFU group. The ablation vol-ume and the energy efficiency factor (EEF) of the 32 ablated rabbits were further analyzed. MRI and pathology were used to compare the changes in the tumor before and after HIFU. The survival of the animals in the HIFU and control groups was also determined. Re-sults: Both pathology and imaging showed that the rabbit liver VX2 tumor adjacent to the abdominal aorta was completely ablated, with an EEF of (25.72±11.40) J/mm3. The survival rate was significantly higher in the HIFU group than in the control group (P<0.05). Conclusion: HIFU ablation is safe and effective in rabbit VX2 liver tumor near the abdominal aorta. This approach significantly in-creases survival.

Objective To study the electrolyte status of 50 infants with severe pneumonia. Method By means of retrospective way to analyze the electrolyte level of blood sample from 50 young children. Results The sequences of electrolyte disturbances in the 50 young children with severe pneumonia were hyponatremia(34%), hypokalemia(30%),hypocalcemia(18%),hypochloremia(16%),hypernatremia(12%),and heperkalemia(10%). Conclusion Young children with severe pneumonia often have electrolyte disturbances of various kinds at the same time; electrolyte evaluations of blood samples from such young children should be done routinely, so that problems can be found early, and correcting measures be taken in time.

Objective To investigate the reproductive outcome after high intensity focused ultrasound (HIFU ) ablation for pa-tients with adenomyosis who have fertility requirements .Methods From October 2010 to June 2013 ,38 patients with adenomyosis who have fertility requirements treated by HIFU in our hospital were analyzed .Results In 38 cases ,the menorrhagia severity score ,the dysmenorrhea pain score and adenomyosis lesions after HIFU ablation were significantly different from the levels before HIFU treatment(P<0 .05) .Successful pregnancy occurred in 15 patients ,full-term birth was seen in 7 patients(three of Cesarean section ,four of vaginal delivery);the remaining patients accept routine prenatal examination .All pregnant patients showed no ab-normality .Conclusion Although large sample size study with long-term follow up is need ,based on our results ,HIFU ablation for patients with adenomyosis might improve the pregnancy rate safely .

Objective To determine the association of acanthosis nigricans(AN) with values for estimating insulin resistance and adipocytokine levels in simple obese children. Methods These subjects concluded 38 simple obese children. AN was present in 17 of 38 children. Height, weight and abdominal circumference were recorded and fasting blood sample was obtained for measurement of blood glucose, plasma insulin, leptin, and plasminogen activator inhibitor 1 (PAI-1) levels which were known as the risk factors of cardiovascular disease. We obtained homeostasis model assessment insulin resistance index (HOMA-R) by fasting blood glucose and plasma insulin levels. Results The severity of obesity, abdominal circumference, fasting insulin levels and HOMA-R were significantly higher in obese children to AN compared to those without AN. There was the tendency that children with AN had higher levels of leptin and PAI-1 compared to children without AN. Conclusions These results suggest that obese children with AN may have a risk of cardiovascular disease as well as type 2 diabetes mellitus.

Objective To explore the age-and sex-specific body composition of normal children in Beijing area.Metheds The subjects were a total of 587 children of 6-14 years old,who were recruited from Beijing schools.All of them had relative weight within normal range(80%～120%),and no chronic disease.The relative weight was obtained,according to standard weight,using the follo-(wing) formula: relative weight(%)=(body weight/standard weight) ?100.Body compositions were estimated with a bioelectrical impedance analyser,which had been proved to be reliable and valid for determining the percentage of body fat.Results Not only fat free mass(FFM) but also fat mass(FM) increased monotonically with age in both sexes.FFM was higher in boys than that in girls at all ages.FM was significantly higher in girls than that in boys aged 6 to 8 years old;however,there was no significant difference for FM between sexes aged 9-14 years old.Patterns of change in mean ratio of body fat(%BF),with age differed by sex.Percent age of BF was significantly higher in girls than that in boys at all ages except at 10 and 11 years old. In boys,%BF increased with age,while in girls it remained nearly constant from age 6 to 10 years old,and gradually increased from age 10 to 14 years old.Body mass index(BMI) increased steadily with age in both sexes,and boys had consistently higher BMI than girls.In boys,the increase in BMI was steeper from age 10 to 14 years old.Even in the subjects with BMI

Objective To investigate dyslipidemia and the use of lipid-lowering drugs in patients with chronic kidney diseases(CKD)and analyze the related risk factors.Methods A total of 397 out-patients with CKD in our Renal Department from March 2006 to March 2007 were enrolled;258 healthy people with similar age and sex were taken as the control group.Lipid levels were compared between the two groups and related factors were analyzed.Results Among 397 CKD patients,there were 52.1% patients with high triglyceride(TG),41% with high total cholesterol(TC),18.8% with low high-density lipoprotein cholesterol(HDL-C),47.6% with high low-density lipoprotein cholesterol(LDL-C).Compared with controls,rates of hypertriglyceridemia and low high-density lipoprotein cholesterol in CKD group were higher(P

Objective To explore the effect of oxytocin on uterine fibroids treated by ultrasound ablation. Methods Eighty-two single points in 29 uterine fibroids from 26 patients were sonicated with magnetic resonance imaging guided by high intensity focused ultrasound before and after using oxytocin. The required total energy, sonication time required to reach 60 ℃ and the acoustic energy for increasing 1 ℃ of temperature at the single point before and after using oxytocin were compared. Results Before intravenous infusion of oxytocin, the average total sonication energy required to reach 60 ℃ was (5320 ±910) J and it took (21 ±20) seconds for sonicating a single point, the energy required for increasing 1 ℃ was (255 ± 302) J. In contrast, after intravenous infusion of oxytocin, the average total sonication energy required to reach 60 ℃ was (2890 ±325) J, and it took (12 ±7) seconds for sonicating a single point, the energy required for increasing 1 ℃ was ( 126 ± 94 ) J. Those three index all reached statistical difference ( P = 0.002, P = 0.001, P= 0.002, respectively). Conclusion It seemed that Oxytocin could significantly decrease the energy required for ablating uterine fibroids, shorten treatment time and improve the treatment efficiency.

To study the quantitative structure-activity relationship (QSAR) between the stuctures of 29 flavonoids and the inhibitory activity of their multidrug resistance-associated protein (MRP) 1 and 2 by using the comparative molecular similarity index analysis (CoMSIA). By studying the impact of the combination of different molecular force fields, researchers obtained the molecular force fields that played an important role in inhibiting the activity of MRP1 and MRP2, built the optimized QSAR model, and discussed the structural modification method for flavonoids' multidrug resistance-associated protein inhibitor. The results of the study could not only provide the guidance for new drug R&D, but also help partially discuss the synergy mechanism between MRP1 and MRP2 receptors and traditional Chinese medicines containing flavonoids.
Drugs, Chinese Herbal ; chemistry ; pharmacology ; Flavonoids ; chemistry ; pharmacology ; Humans ; Models, Molecular ; Multidrug Resistance-Associated Proteins ; antagonists & inhibitors ; chemistry ; Quantitative Structure-Activity Relationship

Objective The aim of this study is to establish a practical VX2 tumour model less than or equal to 10 mm from large blood vessel(as standard) for HIFU ablation .Methods 15 New Zealand rabbits were involved ,VX2 tumour blocks were inoculated near postcava through spine path ,and tumour rate was observed two weeks later by anatomy and MRI .Results Three experimental rabbits did not survive ,all the rest of the 12 into the tumour ,assembly tumour rate was 100% (12/12);The tumour rate reaching the standard accounted for 75% (9/12) ,the average distance between the tumour and the inferior vena cava was (5 .6 ± 3 .4)mm . Conclusion It is feasible to establish the VX2 tumor model less than or equal to 10 mm from large blood vessel through spine path .

Objective To evaluate the feasibility of high intensity focused ultrasound (HIFU) associated microbubble-enhancing contrast (“SonoVue”) therapeutic ablation of uterine fibroids in patients with pelvic operation history. Methods A total of 100 patients with uterine fibroid from October 2010 to December 2012 in Suining Central Hospital of Sichuan were treated with HIFU associated SonoVue. Among them, 50 patients had pelvic operation history. The no-perfused volume, median fractional ablation, ablation power rate, ablation dose, treatment time and adverse reaction were recorded. Results There was no statistical significance between patients with operation history and without in no-perfused volume and ablation does. The ablation power was lower in patients with operation than without. The treatment time and room time were longer in patients with operation than without. The adverse reactions were higher in patients with operation than without, especially skin pain and lower abdominal pain ( <0.05) . According to short- and long-term follow-up results, no major complications were observed in any patients. Conclusion Pelvic operation history increases the difficulty in treatment of HIFU ablation uterine fibroids. HIFU associated contrast agent SonoVue ablation can be considered a safe and feasible approach to the management of uterine fibroids in patients with pelvic operation history after grasping the indications and contraindications.