Objective To investigate the method of handmade microwire lasso device for the management of coil migration during intracranial aneurysm embolization. Methods Two migration coils were removed from intracranial arteries using handmade microwire lasso rings. The first coil prolapsed completely from an aneurysm sac and flowed to the middle cerebral artery M2 segment. The second coil partially prolapsed out into a parent artery. The microwire lasso device was made of 4-0 silk thread and fixed using 0. 36 mm (0. 014 inch)microwire and 0. 43 mm (0. 017 inch)microcatheter. Results After removing 2 migration coils from intracranial arteries,angiography did not reveal vascular injury and thrombosis. There was no aneurysm rupture. After awaking from anesthesia,the patient did not have neurological deficit. The patient was followed up for 3 months after procedure. MR angiography confirmed the patency of related arteries. Conclusion The handmade microwire lasso device for the management of coil migration in the process of aneurysm embolization is a simple,effective,and economical method.

BACKGROUND:5-Fluorouracil is a common chemotherapy drug for gastric cancer, but it is more likely to develop drug resistance in clinical treatment. Studies have shown that tumor stem cels are lowly sensitive to chemotherapeutic drugs, which may be an important cause of chemotherapy resistance. OBJECTIVE:To analyze the sensitivity of gastric cancer stem cels to 5-fluorouracil in vitro, and to understand the mechanism of drug resistance associated with gastric cancer. METHODS:Based on the sorting strategy, human gastric cancer cel clones were isolated from AGS cel lines. CD44 and thymidylate synthetase expression in different clones was detected using immunocytochemistry analysis. Clone formation assay was used to evaluate self-renewal capacity of different clones. Cel counting kit-8 was used to determine the clonal growth inhibition rate of AGS under treatment with different concentrations of 5-fluorouracil. RESULTS AND CONCLUSION:The AGS cels that were inoculated with low density and cultured could differentiate into 32 forms, including the ful clone (16%, 5/32), the second clone (66%, 21/32) and the accessory clone (19%, 6/32). Among them, the ful clones highly expressed CD44 and thymidylate synthetase, and could generate a great amount of passage 2 clones after inoculation; the second clones showed a weak expression of CD44 and thymidylate synthetase, and could generate a smal number of passage 2 clones after inoculation; the accessory clones showed a weak or no expression of CD44 and thymidylate synthetase, and there was no passage 2 clone after inoculation. Under the effect of different concentrations of 5-fluorouracil, the growth inhibition rates of the secondary clones and AGS cels were both higher than that of the ful clones (bothP < 0.05). These findings indicate that gastric cancer stem cels have a relatively lower sensitivity to 5-fluorouracilin vitro, which is speculated to be an important mechanism of drug resistance.

Objective To investigate the influencing factors for late-preterm birth of twins. Methods We retrospectively analyzed the clinical data of 301 twins delivered in Beijing Haidian Maternity and Child Health Hospital between August 1, 2011 and August 31, 2013. Twins with late-preterm births were classified as the observation group(n=138) and those delivered at term were classified as the control group (n=163). Comparison between the two groups was conducted on both maternal and neonatal complications. Two independent sample Student's t test, Chi-square test and logistic regression analysis were performed for statistical analysis. Results (1) The ratio of two male fetuses, dichorionic diamniotic twins, and iatrogenic preterm birth were 41.3% (57/138), 76.1% (105/138) and 65.2% (90/138), respectively, in the observation group, while 23.9% (39/163), 89.6% (146/163) and 96.9% (158/163), respectively, in the control group (χ2=10.40, 9.81 and 53.59, respectively, all P<0.05). Mean birth weight of twins in the observation group was less than that in the control group [(2 450±349) vs (2 640±304) g, t=7.12, P<0.05]. Maternal age, gravidity, parity, history of abortion, mode of conception and 1 min Apgar score of the neonates between the two groups were not significantly different (all P>0.05). (2) The risk factors for late-preterm birth of twins included hypertension diseases complicated pregnancy, gestational diabetes mellitus, premature rupture of membranes, fetal distress, anemia and umbilical cord abnormality [OR=0.190(95%CI: 0.094-0.387), 1.980 (95%CI: 1.009-3.883), 0.030(95%CI: 0.007-0.131), 0.062(95%CI: 0.006-0.642), 0.470(95%CI:0.243-0.908) and 2.779(95%CI:1.093-20.736), respectively, all P<0.01 or 0.05]. (3) The incidences of hyperbilirubinemia, respiratory diseases, hypoglycemia, anemia, and neonatal infection were 27.9%(77/276), 4.4%(12/276), 4.7%(13/276), 4.4%(12/276) and 3.6%(10/276), respectively, in the observation group, which were all higher than those in the control group [9.5%(31/326), 0.6%(2/326), 1.2%(4/326), 0.6%(2/326) and 0.3%(1/326), respectively,χ2=34.33, 9.18, 6.61, 9.18 and 9.16, respectively, all P<0.05]. The incidence of small for gestational age in the observation group was lower than that in the control group [3.6%(10/276) vs 12.3%(40/326),χ2=3.86, P<0.05]. Other complications in the two groups (intracranial hemorrhage, acidosis, and hemolysis) were not significantly different (all P>0.05). Conclusions Late-premature births in twin pregnancies are related to many maternal and fetal risk factors. Therefore, clear understanding of these risk factors might improve the pregnancy outcomes.

Objective To explore the clinical significance of oral pancreatin capsules for patients after total gastrectomy.Methods Ninety cases of total gastrectomy patients in Nanyang City Center Hospital were selected and randomly divided into trypsin group and control group,with 45 patients in each group.The control group were treated by conventional therapy,and the patients of trypsin group were treated by pancreatin capsules on the basis of conventional therapy after 3 weeks postoperation.Scores of EORTC QLQ-C30,korenaga and fecal fat contents were compared between two groups six months postoperation.Results No statistically significant difference was found in the scoring system of EORTC QLQ-C30,korenaga and fecal fat contents in two groups before administered,The score of role function,emotional function and overall health status in the scoring system of EORTC QLQ-C30 of patients in trypsin group were higher than control group after 6 months administration.The differences were statistically significant (P<0.05 ).The score of fatigue,loss of appetite,nausea,vomiting,diarrhea in the scoring system of EORTC QLQ-C30 of patients in trypsin group were lower than control group after 6 months administration.The differences were statistically significant (P<0.05).The loss of appetite,swelling postprandial satiety,insomnia and the extent of weight loss in the scoring system of korenagain trypsin group were lower than control group after 6 months administration.The differences were statistically significant (P<0.05 ).The fecal fat contentsin trypsin group were lower than control group at 24 h and 72 h.The differences were statistically significant (P<0.05).The cases of 24h fecal fat content>7g in trypsin group was lower than control group at 24 h.The differences were statistically significant (P<0.05).Conclusion Oral pancreatin capsules could significantly improve the quality of life of patients after total gastrectomy, which is worthy of promoting.

Objective: To establish an HPLC method for the determination of diclofenac sodium sustained-release preparations, and compare the in vitro dissolution of diclofenac sodium sustained-release capsules and tablets from different manufacturers to provide reference for the clinical reasonable medication. Methods: According to the first dissolution method described in Chinese Pharmaco-poeia (2010 edition),the dissolution of each sample was determined by HPLC and the cumulative release percentage was calculated. The dissolution parameters were fitted by Weibull-equation and the results were analyzed. Results:The HPLC method for the determi-nation of diclofenac sodium release was accurate with good reproducibility, and the dissolution parameters of different manufacturers had remarkable difference. Conclusion:The in vitro dissolution of diclofenac sodium sustained-release capsules and tablets from different manufacturers is various, which should be paid attention in the clinical use.

Aim To investigate the effect of cisplatin(CDDP) on toxicity and apoptosis of human luteinized granulose cells.Method Human luteinized granulose cells were prepared from oocyte retrieval of fertilization procedure in vitro and treated with various concentrations of cisplatin(0,0.5,1.0,2.5,5.0 mg?L-1).The inhibitory rate of cells was examined by MTT assay.The change of nucleolus stained with Hochest 33258 was observed under fluorescent microscope,and bcl-2 and bax protein expressions were detected by flow cytometry(FCM).Results The results showed that CDDP inhibited the growth of ovarian granulose cells at the concentration of no less than 1 mg?L-1,in which Hochest 33258 staining demonstrated cell apoptosis.The expression of bax protein increased as compared with that of the control(P

Objective:To observe effects of Panax pseudo-ginseng saponins on protein expression of VEGF, bFGF in myocardium in acute myocardial infarction rats. Methods: The acute myocardial infraction (AMI) model was established in one hundred and forty Wistar rats, and the rats were randomly divided into five groups: the western medicine group mobilized by subcutaneous injection of G-CSF50?g.kg-1.d-1, sham-operated group and model group treated by subcutaneous injection of normal saline 50?g.kg-1.d-1, the Chinese medicine group mobilized by intraperitoneal injection of Xuesaitong (ingredients of Panax pseudo-ginseng saponins) 150mg.kg-1.d-1, the integrative group mobilized by subcutaneous injection of G-CSF 50?g.kg-1.d and intraperitoneal injection of Xuesaitong 150mg.kg-1.d-1. Except for the sham-operated group, each group was divided into three sub-groups by three time points of 1d, 7d and 14d. G-CSF was used once a day for 7 days. Xuesaitong was injected once a day until the rats were killed. The parameters cardiac function in rats with myocardial infarction were detection by color doppler ultrasonic diagnostic apparatus in different times,the expression of VEGF, bFGF in Marginal zone of myocardium in rats with myocardial infarction were detected by immunohistochemistry in different time. Pathological and ultrastructural changes of marginal zone in rats with acute myocardial infarction were observed by electron microscopy and light microscopy. Results:Panax pseudo-ginseng saponins can improve the level of left ventricular systolic function such as EF,FS,it can inhibit the increase of left ventricular LVDD and LVDS,it can improve expression levels of VEGF,bFGF of marginal zone in acute myocardial infarction rats. It also can reduce the damage to cell ultrastructure and promote revascularization in the site around MI area. Panax pseudo-ginseng saponins can protect the myocytes in rats with myocardial infarction. Conclusion:Panax pseudo-ginseng saponins can protect myocardium from ischemic injury in rats after AMI by way of improving expressions of VEGF and bFGF in myocardial cells and promoting angiogenesis in the infarcted of myocardium.

Objective:To determine whether the bcl-2 antisense oligonucleotide (ASODN) can increase the sensitivity of NCI-H460 cell lines to docetaxel or radiation. Methods: Docetaxel in combination with bcl-2 ASODN or non-sense oligonucleotide (NSODN) was used to treat NCI-H460 cells, and then IC_~50 of docetaxel against NCI-H460 cells was determined with MTT method. In radiation group, NCI-H460 cells were divided into pure radiation, radiation plus bcl-2 ASODN, and radiation plus NSODN groups. The inhibition rates of cell growth were assayed by MTT, and the expression levels of Bcl-2 protein were assayed by immunofluorescence. Results: IC_~50 in bcl-2 ASODN plus docetaxel, pure docetaxel and NSODN plus docetaxel groups was (0.101?0.009) ?mol/L,(0.183?0.018)?mol/L, and (0.179?0.016)?mol/L, respectively. The differences between the first group and the latter 2 groups were significant (P

Objective To evaluate the selenium nutrition level of Huaibei inhabitants and to supply valid data to Chinese Microelement Nutrition Database. Methods 513 healthy people living in Huaibei for more than 10 years were selected randomly from September 2003 to June 2004. The selenium content in hair was measured by catalytic polarographic method to delimit selenium richness region. According to the selenium content in hair,the intake of selenium from food was estimated. The investigation results were evaluated by the standard of Chinese Dietary Reference Intakes. Results The selenium content in hair was (0.390 3?0.104 5) ?g/g in Huaibei inhabitants,only 51.9% of the people reached the proper level. The average intake of selenium of Huaibei inhabitants was (31.92?9.93) ?g/d,which showed that the selenium nutrition level of Huaibei inhabitants was lower than the standard of Chinese Dietary Reference Intakes except children between 0 to 6 years old. The percent which satisfied RNI and EAR standard occupied 19.9% (102/513) and 32.9% (169/513) respectively,which were also much lower than the 50% of physiological necessary level. Conclusion The selenium intake from food in Huaibei inhabitants was scarce obviously. To increase the selenium intake properly will be a wise selection.

Objective To study the value of imaging characters in diagnosing mediastinal tumors.Methods X-ray and CT findings of pathologically proved mediastinal tumors in 58 cases were analysed,the findings enabling qualitative diagnosis were recommended. Results There were 40 cases of anterior mediastinal tumors including intrathoracic thyroid tumors [n=32,5 tyroid carcinomas and 1 thyroid cyst ,thymomas (n=3) and teratomas (n=5, 3 cases ruptured)]. All the other 18 cases in posterior mediastinum were neurogenic tumors.Conclusion Based on the combination of the mediastinal compartment knowledge, X-ray and CT findings of the tumors and the clinical information, the qualitative diagnosis of mediastinal tumors will be characterized correctly.