2.A study of kinematic parameters of normal youth in gait analysis
Zhong-wu GUO ; Guang-zhi WANG ; Hui DING ; Jing GAO ; Haishu DING
Chinese Journal of Rehabilitation Theory and Practice 2002;8(9):532-533
ObjectiveTo study basic pattern of normal youth in gait analysis.Methods28 healthy young persons as experimental subjects were studied. The gait analysis system based on digital video and digital image processing was employed to obtain kinematic parameters.ResultsThe means and standard deviations of kinematic parameters of normal youth were obtained. The differences of gait patterns between males and females were compared. The correlations between gait parameters and height, weight were analyzed respectively.Conclusions It is recommended that the values of the gait parameters and the trends obtained can be used as a standard gait pattern of normal youth. Statistically significant differences exist between the males and the females for most of the gait parameters. Furthermore, the differences of the values of gait parameters between the males and the females are not led only by the difference of height except very few parameters.
3.Role of angiotensinⅡin the changes of MMP-9 and TIMP-1 mRNA expressions in high glucose-induced mesangial cells of rat
Hai-Yan LI ; Ying GUO ; He-Lin DING ; Zu-Zhi FU ;
Chinese Journal of Endocrinology and Metabolism 1985;0(02):-
High glucose stimulated angiotensinⅡ(ATⅡ) production in mesangial cells of rat.Both high glucose and ATⅡdecreased expressions of matrix metalloprotein-9 (MMP-9) mRNA and MMP-9/tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA ratio and increased TIMP-1 mRNA expression in mesangial cells of rat,which could be reversed by an ATⅡreceptor blocker,Saralasin.
4.Effects of Shenqi Fuzheng injection on gene expression profile of liver tissue with metastatic carcinoma in mice.
Zhi-guo DING ; Nai-qing LI ; De-sheng TAO
Chinese Journal of Integrated Traditional and Western Medicine 2008;28(2):135-138
OBJECTIVETo explore the effect of Shenqi Fuzheng Injection (SFI) on gene expression profile of liver tissue with metastatic carcinoma in mice.
METHODSTwenty liver metastatic carcinoma model mice were established by splenectomy after their spleens were injected with 0.2 mL colon cancer C26 cell strain oncocyte liquid, then they were randomly divided into the model group and the SFI group. Starting from the 5th day after modeling, mice in the model group and the SFI group were given via intraperitoneal injection once every other day with physiological saline and SFI respectively. All the mice were sacrificed at the 15th day and the gene profile of metastatic liver carcinoma tissue in the two groups were screened by whole genome chip technique.
RESULTS(1) The model establishing successful rate reached 100%; (2) Gene expression showed that as compared with the model group, in the SFI group, 123 genes were up-regulated, with 52 of them registered to Ensemble, while only one gene was down-regulated and registered to Ensemble was none.
CONCLUSIONSFI plays its role of anti-tumor mainly by upregulating several relative genes to promote apoptosis of tumor cells and stabilizing chromosomes.
Animals ; Cell Line, Tumor ; Cluster Analysis ; Colonic Neoplasms ; pathology ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; drug effects ; Injections, Intraperitoneal ; Liver ; drug effects ; metabolism ; pathology ; Liver Neoplasms ; drug therapy ; genetics ; secondary ; Male ; Mice ; Neoplasm Transplantation ; Phytotherapy ; Random Allocation
5.Research advances on the pathogenesis of acute pancreatitis
Zhi ZHENG ; Yuanxu QU ; Yixuan DING ; Wentong MEI ; Yuchen JIA ; Yulin GUO ; Feng CAO ; Fei LI
Chinese Journal of Hepatobiliary Surgery 2021;27(2):152-155
Acute pancreatitis (AP) is an inflammatory disease of the pancreas. Its pathogenesis is not only related to abnormal activation of trypsinogen, but also related to calcium overload, mitochondrial dysfunction, impaired autophagy and endoplasmic reticulum stress. However, the mechanism has not been fully elucidated and needs to be further studied. Currently, there is no effective treatment for AP. It is difficult to prevent the loss of pancreatic function. An in-depth understanding of the pathophysiological mechanisms of AP may help to identify the potential therapeutic targets. Therefore, the purpose of this study is to review recent advances in the mechanism of AP in order to provide more research direction for treatment.
6.Evaluation of myocardial blood supply using adenosine stress myocardial perfusion imaging
Qian, WANG ; Ji-min, YANG ; Hong-zhi, MI ; Fang, GUO ; Jian, JIAO ; Jing-jing, MENG ; Jian, DING ; Yao, LU
Chinese Journal of Nuclear Medicine 2010;30(6):363-366
Objective To retrospectively evalute the value and accuracy of adenosine stress and rest SPECT myocardial perfusion imaging. Methods A total of 1858 patients who were suspected or known for coronary artery disease (CAD) underwent 99Tcm-methoxyisobutylisonitrile ( MIBI ) myocardial perfusionSPECT with adenosine infusion using the standard 2-day protocol. Images were interpreted by two or more experienced nuclear medicine physicians . Coronary angiography was carried out in all patients within one month. Kappa test was used to analyze the correlation between the two imaging studies. Results By coronary angiography, there were 957 patients diagnosed of CAD (one-, two-, three-vessel disease: 506,256,195, respectively) and 901 normal. Stenosis was found in 1603 vessels, including left anterior descending coronary artery (LAD): 765, left circumflex coronary artery (LCX): 399 and right coronary artery (RCA): 439. By adenosine induced stress myocardial perfusion imaging, 876 patients were diagnosed of myocardial ischemia ( sensitivity: 876/957, 91.54% ) and 651 patients had negative findings ( specificity:651/901,72.25 % ). The positive and negative predictive values were 77.80% ( 876/1126 ) and 88.93% (651/732), respectively. The correlation coefficient between the two imaging studies was 0.641. The vessel-based sensitivity was 81.31% (622/765) for LAD, 56.64% (226/399) for LCX and 70.62% (310/439) for RCA, respectively. The sensitivity for detection of one-, two-, three-vessel stenosis was 87.55% (443/506), 94.92% (243/256) and 97.44% (190/195), respectively. The side-effects was mild and transient with an incidence rate of 84.12% ( 1563/1858), without major cardiac events. Conclusion Stress myocardial perfusion imaging induced by adenosine is reliable for the evaluation of myocardial blood supply in CAD patients.
7.Study on preparation of andrographolide composite particles based on mechanical crushing method and its dissolution.
Xiao-Ping WANG ; Li HAN ; Gui-Lin REN ; Ding-Kun ZHANG ; Zhen-Feng WU ; Ming YANG ; Zhi-Ping GUO
China Journal of Chinese Materia Medica 2014;39(4):657-662
OBJECTIVETo prepare andrographolide composite particles, and evaluate their particle structure and dissolution.
METHODThe mechanical crushing method was adopted to prepare andrographolide and polyethylene glycol (PEG) 6000 composite particles. The structures were characterized by the scanning electron microscope (SEM) and the differential scanning calorimeter (DSC). The contact angles were determined by the contact angle analyzer. The in vitro dissolution curve was detected.
RESULTAndrographolide and PEG 6000 gave rise to coated composite particle structures, with the decrease in the crystallinity of andrographolide. The in vitro dissolution rate of composite particles was significantly obvious than that of its raw materials, ultrafine powder and their physical mixtures.
CONCLUSIONAndrographolide composite particles based on the mechanical crushing method could notably enhance the in vitro dissolution of andrographolide.
Calorimetry, Differential Scanning ; Chemistry, Pharmaceutical ; methods ; Diterpenes ; chemistry ; Drugs, Chinese Herbal ; chemistry ; Particle Size ; Solubility ; Torsion, Mechanical
8.Intratympanic versus systemic steroid treatment for idiopathic sudden hearing loss: a meta-analysis.
Xiu-yong DING ; Ting-ting CUI ; Guo-dong FENG ; Zhi-qiang GAO
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2013;48(5):412-416
OBJECTIVETo assess the effectiveness and safety of intratympanic versus systemic steroid therapy in the initial treatment of idiopathic sudden hearing loss.
METHODSAn extensive search of the literature was performed in Pubmed and other available database from January, 1980 to November, 2011. After filtering by the criteria of Cochrane Collaboration, a meta-analysis was conducted.
RESULTSNine studies met the criteria for meta-analysis, for idiopathic sudden hearing loss patients without diabetes received intratympanic steroid therapy, the improvement rate (RR = 1.11,95% CI = 0.96-1.28, P = 0.15) did not show any significance when compared with the patients received systemic therapy. While a significant difference of improvement rate occurred between intratympanic and systemic steroid therapy in the idiopathic sudden hearing loss patients with diabetes (RR = 1.24, 95% CI = 1.02-1.50, P = 0.03).
CONCLUSIONFor the initial therapy of idiopathic sudden hearing loss patients without diabetes, systemic steroid treatment still remains the first choice, but for the idiopathic sudden hearing loss patients with diabetes, intratympanic steroid treatment should be used for the initial treatment.
Administration, Oral ; Audiometry, Pure-Tone ; Dexamethasone ; administration & dosage ; therapeutic use ; Hearing Loss, Sensorineural ; drug therapy ; Hearing Loss, Sudden ; drug therapy ; Humans ; Methylprednisolone ; Steroids ; administration & dosage ; therapeutic use ; Treatment Outcome ; Tympanic Membrane
9.Percutaneous pinning for the treatment of proximal fractures of humerus.
Shao-Long DING ; Zhi-Dun GUO ; Zi-Li WANG
China Journal of Orthopaedics and Traumatology 2009;22(5):363-364
Adolescent
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Adult
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Aged
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Child
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Child, Preschool
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Female
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Fracture Fixation, Intramedullary
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methods
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Fractures, Bone
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surgery
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Humans
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Humerus
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surgery
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Male
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Middle Aged
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Treatment Outcome
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Young Adult
10.Research progress of Kruppel-like factor family in malignant tumors
Zhi ZHENG ; Yixuan DING ; Wentong MEI ; Yulin GUO ; Yuanxu QU ; Jiongdi LU ; Shuang LIU ; Haichen SUN ; Feng CAO ; Fei LI
International Journal of Surgery 2021;48(4):264-268
Malignant tumors usually have no obvious clinical symptoms in the early stage. Most patients are already in the advanced stage when they are diagnosed. Some patients have lost the opportunity for operation, resulting in poor prognosis. Therefore, how to find the best therapeutic target for such patients and improve the prognosis of patients has gradually become the focus of scholar′s attention. Recently, Kruppel-like factor (KLF) is a transcriptional regulator that can bind to the target DNA, and its family plays an important role in the occurrence and development of malignant tumors. It has also been confirmed that the KLF family affects the proliferation, differentiation and migration of tumor cells, but the specific mechanism is still not fully elucidate. Consequently, in order to further explored the effect of the KLF family on tumors, this study intends to briefly review the roles and regulatory mechanisms of the KLF family in the cell proliferation, differentiation and migration of malignant tumors, hoping to provide new target for the biological treatment of tumors.