As a new concept, the definition of translational medicine remains obscure. The translational medicine connects the bench to bedside, and its importance would be more remarkable. The development of gastrointestinal surgery reflects the idea of translational medicine. To carry out the translational study, the gastrointestinal surgeon must learn how to find subjects from clinical problems, how to collect complete information and tissues, how to collect complete information and tissues, how to collaborate with others from different fields and how to utilize all kinds of resources. By translational studies, gastrointestinal surgeons may further improve the survival of patients with gastrointestinal tumor.
Gastrointestinal Neoplasms ; Humans ; Translational Medical Research

Humans ; Oncogenes ; Stomach Neoplasms ; genetics

Gastrointestinal Diseases ; physiopathology ; Gastrointestinal Motility ; Humans ; Kinetics ; Postoperative Complications

Objective To investigate the clinical effect of Clostridium butyricum powder in treating infantile indigestion diarrhea.MethodsFrom February 2013 to October 2015 in our hospital 76 cases of pediatric indigestion diarrhea as the research object, randomly divided into experimental group and control group with 38 cases in each group, two groups were given Bao,er ning granule, combined group plus live bacteria powder for the treatment of Clostridium butyricum, three days for a course of treatment, continuous treatment of two courses.ResultsBefore treatment, there was no significant difference in clinical symptom scores between the experimental group and the control group,after treatment,the scores of loss of appetite, abdominal pain, diarrhea, abdominal distension and stool of the experimental group were lower than those of the control group, the difference was statistically significant (P<0.05);Before treatment, there was no significant difference in serum motilin, gastrin and somatostatin levels between the experimental group and the control group.After treatment, the levels of serum motilin and gastrin in the experimental group were lower than those in the control group, and the level of serum somatostatin was higher than that of the control group, the difference was statistically significant (P<0.05);After treatment, the total effective rate of experimental group was 92.11% higher than that of control group 73.68%, the difference was statistically significant (P<0.05).ConclusionClostridium butyricum powder with Bao,er ning granule in the treatment of infantile dyspeptic diarrhea effect is good, worthy of clinical application.

Some problems were settled in making permanent slide for predacious nematode fungi by slide cultivation and cotton blue stain. Method of small-hole culture covered with slides could obtain high quality images, which solved the problem that trapping device couldn't be observed with high-power microscopes and oil-immersion microscopes. Scraping slide technique improved the method of making temporary slides.

Robotic system helps surgeons in performing surgery. Currently Da Vinci system is the most popular. Da Vinci system has been used for the stomach and bowel diseases in 27 cases(18 cases of stomach and 9 cases of colon and rectum) in the Zhongshan Hospital, Fudan University. Accurate preoperative staging is crucial, and Da Vinci system is advantageous in lymph node dissection, preservation of nerve plexus, and complete resection of mesorectum. Adoption of gastrointestinal tract reconstruction technique should depend on the operation and experience in surgery. Though Da Vinci system has limitations and the cost is high, it is believed to be the future trend.
Digestive System Surgical Procedures ; methods ; Humans ; Robotics ; methods

Adult ; Empyema, Pleural ; complications ; etiology ; Humans ; Male ; Neck Injuries ; complications ; Tracheoesophageal Fistula ; complications ; etiology

Adult ; Carotid Artery, External ; anatomy & histology ; Humans ; Hypoglossal Nerve ; anatomy & histology ; Tongue ; anatomy & histology ; blood supply ; innervation

Objective:To establish a multidrug resistant human salivary gland adenoid cystic carcinoma cell line.Methods:Human salivary gland adenoid cystic carcinoma cell line ACC-2 was treated by 24-hour-exposure to high dose of Bleomycin(BLM)(20 ?g/ml).Drug sensitivity was evaluated by MTT assay.Cell counting was employed to make the growth curve and to calculate the cell doubling time.Flow cytometry(FCM) was used to study the cell cycle distribution and apoptosis.The colony formation ability was also observed.Results:Multidrug resistant cell line of human salivary gland adenoid cystic carcinoma was established and named ACC-2/BLM.After 10 times repeated exposure to BLM,the resistance index(RI) to BLM,5-Fluorouracil(5-FU),Cisplatin(CDDP),Cyclophosphamide(CTX),Vincristine(VCR) were 7.299,1.03,2.15,1.114 and 5.96 respectively.Compared with ACC-2,the proliferation potential of ACC-2/BLM cells was decreased.The ACC-2 apoptosis cells were much more than ACC-2/BLM cells after 9-day-treatment by BLM at 60 ?g/ml.Conclusion:ACC-2/BLM cell line has multidrug resistant characteristics.

Objective To evaluate living-related liver transplantation (LRLT) for the treatment of Wilson′s disease. MethodsBetween Jan. 2001 and Oct. 2003,LRLT was performed in 20 patients (mean age 11.1 years) of late staged Wilson′s disease. Among them emergency transplantation was performed in 3 patients. Donor livers were all from patient′s parents. ResultsSurgery was successful in all donors and recipients, liver function test and serum ceruloplasmin reached normal level one month after transplantation. One patient died of severe rejection. Discharged patients were followed up from 2～33 months (mean 18.9 months). [WT5”HZ]ConclusionsLiving related liver transplantation is an effective treatment for Wilson′s disease complicated with hepatic dysfunction.