Apoptosis ; Cell Proliferation ; DNA Methylation ; Humans ; Intercellular Signaling Peptides and Proteins ; genetics ; metabolism ; Membrane Proteins ; genetics ; metabolism ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasms ; metabolism ; pathology ; Neovascularization, Pathologic ; Nerve Tissue Proteins ; genetics ; metabolism ; Promoter Regions, Genetic ; Receptors, Immunologic ; genetics ; metabolism ; Signal Transduction

Animals ; Breast Neoplasms ; metabolism ; Humans ; NF-kappa B ; metabolism ; Neoplasms ; metabolism ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Receptors, Notch ; metabolism ; physiology ; Signal Transduction

Objective To study the clinical and pathological characteristics, treatment results and prognosis of primary parotid non-Hodgkin's lymphoma (NHL). Methods All of our 21 patients received operative resection first and were histologically comfirmed as having T-cell (intermediate grade) and 20 B-cell lineage (2 high grade, 6 intermediate grade ,12 low grade) including 7 mucosa-associatied lymphoid tissue (MALT) phenotype. Ann Anbor stages were 16 stage Ⅰ E and 5 stage Ⅱ E lesions. All patients were treated by radiotherapy,of them 11 were also given 2～6 cycle chemotherapy. Results The overall 5-year survival rate was 77.0%. All 5 patients who died did so of distant involvement. Conclusions Our data show that MALT lymphoma can be present as a primary paroid NHL,although it is not included in the Working Formulation. Low-dose radiotherapy is of choice in the treatment. Patients with intermediate or high grade NHL should be given multi-modality therapy.

Antineoplastic Agents, Alkylating ; pharmacology ; Apoptosis ; drug effects ; Benzimidazoles ; pharmacology ; DNA Repair ; Dacarbazine ; analogs & derivatives ; pharmacology ; Drug Resistance, Neoplasm ; drug effects ; physiology ; Humans ; Membrane Proteins ; metabolism ; O(6)-Methylguanine-DNA Methyltransferase ; antagonists & inhibitors ; metabolism ; Poly(ADP-ribose) Polymerase Inhibitors ; Poly(ADP-ribose) Polymerases ; metabolism ; Proto-Oncogene Proteins ; metabolism ; Purines ; pharmacology ; Pyrimidines ; pharmacology ; Tumor Suppressor Protein p53 ; metabolism

Objective:To investigate the functions of the ITSN1-S SH3 domains in U87 glioblastoma cell proliferation and to de-termine the underlying molecular mechanism. Methods: A recombinant lentiviral vector with an mGFP label was constructed. EH1-EH2, EH1-EH2-CC, and ITSN1-S genes were amplified using polymerase chain reaction and then cloned into recombinant lenti-viral vectors. The four lentiviral plasmids were packaged using HEK 293T cells and subsequently used to infect U87 cells. Stable cells were screened using puromycin and separately labeled as vector/U87, EH1-EH2/U87, EH1-EH2-CC/U87, and ITSN1-S/U87. Western blotting was used to detect the expression of each protein. Proliferation and soft agar assays were performed to detect cell proliferation. Results:In the proliferation and soft agar assays, the proliferation capacity of the ITSN1-S/U87 cells was clearly enhanced compared with those of the vector/U87, EH1-EH2/U87, and EH1-EH2-CC/U87 cells (P<0.05). Moreover, the proliferation capacity of the latter three groups showed no observable difference (P>0.05). On the 6th day, the vector/U87, EH1-EH2/U87, EH1-EH2-CC/U87, and ITSN1-S/U87 cell numbers were (29.16 ± 1.19) × 104, (22.82 ± 0.94) × 104, (22.17 ± 0.90) × 104, and (21.93 ± 1.15) × 104, respectively. On the 21st day, the number of colony formation in vector/U87, EH1-EH2/U87, EH1-EH2-CC/U87, and ITSN1-S/U87 was (6.37±0.41)×103, (2.65±0.34)×103, (2.23±0.31)×103, and (2.1±0.29)×103, respectively . Conclusion:ITSN1-S overexpression significantly promotes U87 cell proliferation. Specifically, the SH3 domains possibly serve vital functions in glioma cell proliferation.

Central Nervous System ; pathology ; Humans ; Lymphoproliferative Disorders ; pathology ; Transplantation ; adverse effects

Objective To analyze the STK11 gene mutation in a sporadic Chinese Datient with Peutz Jeghers syndrome(PJS)so as to provide a basis for the genetic diagnosis and counseling of PJS.Methods Whole blood samples were obtained from a female patient with PJS,her parents and sister.as well as from 100 unrelated,normal individuals as control.Genomic DNA was extracted,and the whole coding region of STK11 gene was amplified by PCR followed by direct sequencing.Results Molecular analysis revealed a novel het-erozygous mutation C73S in the patient,which resulted from the substitution of thymine(T)for adenine(A) at codon 217 in exon 1 of STK11 gene.However, the novel mutation was not found in unaffected family mem-bers or unrelated controls.Conclusion A novel missense mutation C73S,which may contribute to the devel-opment of PJS,is found in the patient.

BACKGROUND: The brittleness and low flexural strength of ceramic material hinder, to a certain extent, its application in prosthodontic dentistry. Zirconia is enhanced in its flexural strength and toughness by the transformation toughening mechanism, which makes up for the brittleness of the traditional all-ceramic material.OBJECTIVE: To preliminarily explore a new machinable zirconia ceramic material and investigate sintering properties of dental machinable zirconia/La-monazite diphase ceramics with nano-zirconia/La-monazite diphase ceramics. DESIGN: By adjusting the composition and ratio of raw materials, and by adopting different preparation and sintering method, this study was intended to measure the related parameters and to explore the best preparation and sintering method. SETTING: Department of Prosthodontics, College of Stomatology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University; Lab of Advanced Inorganic Material Technology, School of Material Science and Engineering, Shanghai University. MATERIALS: There were 3 mol yttria-containing tetragonal zirconia polycrystals (3Y-TZP) (size≤50 nm, purity 99.99%, Yixing Xinxing Zirconia-products Co., Ltd.) and La-monazite (purity 99.99%, Baotou Rare-earth Phosphate Institution). METHODS: Experiments were performed at the Department of Prosthodontics, College of Stomatology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University from January 2004 to December 2006. The pilot experiments found that the zirconia with less than 15% of lanthanum phosphate was high in strength but poor in machinability while more than 20% of lanthanum phosphate was decreased significantly in its strength. Therefore, 15%, 18% and 20% (volume percentage) of la-monazite was added to 3Y-TZP. The green bodies of the three groups were compacted by cold isostatic pressing (200 MPa) and were sintered in air atmosphere at different temperatures: 1 560 ℃, 1 580 ℃ and 1 600 ℃ to make the ceramic samples. MAIN OUTCOME MEASURES: The volume density, porous rate (Archimedes method) and three-point bending strength (EZ-100 universal testing machine) were tested of all the ceramic samples.RESULTS: ①With the increase of sintering temperature, zirconia/La-monazite ceramics with 15%, 18% and 20% lanthanum phosphate was increased in its bulk and density. The density was the highest for 1 600 ℃ and the respective density of the three groups were 5.77 g/cm3, 5.42 g/cm3 and 5.39 g/cm3. The porous rate decreased with the increasing temperature and was the lowest at 1 600 ℃ (0.88%, 1.21%, 1.49% respectively). There was no significant difference in volume and density at different temperatures (P > 0.05). ②The flexure strength of diphase ceramic with 18% and 20% lanthanum phosphate increased with the temperature increasing to 1 580 ℃. At 1 580 ℃, the flexural strength reached the highest level, respectively (772.22±43.43) MPa, (216.03±25.20) MPa and (157.21±9.79) MPa. When the temperature reaches 1 600 ℃, the strength was decreased. CONCLUSION: Zirconia/La-monazite diphase ceramics can be prepared by adopting cold isostatic pressing (200 MPa) and sintering at 1 580 ℃.

In this paper,the attachment of a China-made removable clear orthodontics appliances are introduced. The clinical attachment positions and considerations were illustrated with clinical pictures. A typical case was attached for demonstration.

Objective To study the roles of apoptosis genes bcl 2, bax, and bak in the pathogenesis of hepatitis D. Methods Expressions of HDAg, bcl 2, bax, and bak in liver specimens of 77 patients with hepatitis D were studied by immunohistochemical method. Meanwhile, the relationship of HDAg expression with the expressions of bcl 2, bax, and bak was studied by double labelling. Results Bcl 2 was mainly expressed in the cytoplasm of hepatocytes, and bax and bak mainly in the cytoplasm of hepatocytes and partly in the nucleus of hepatocytes, and HDAg mainly in the nucleus of hepatocytes. Lots of HDAg and bax/bak positive cells were distributed in infiltrating lymphocytes at the periportal region especially at the advancing edges of areas of piecemeal necrosis. Apoptosis of many hepatocytes was found to locate near the HDAg positive cells. There was positive correlation between the expression of bax/bak and HDAg expression ( P