1.Curative efficacy of Angelica containing selenium in treatment of chronic renal failure and its effects on Micro-inflammatory state
Fei HUANG ; Wenfeng MA ; Yun ZHANG
International Journal of Laboratory Medicine 2017;38(14):1887-1889
Objective To study curative efficacy of Angelica containing selenium in treatment of chronic renal failure and its effects on Micro-inflammatory state.Methods A total of 80 patients of chronic renal failure who received therapy from March 2014 to March 2015 in our hospital were selected as research objects,and divided into observation group and control group,40 patients in each group.The control group was treated with routine treatment,while the observation group was treated with routine treatment with selenium enriched Angelica.Then renal function indicators including blood urea nitrogen(BUN),serum creatinine (SCr),hemoglobin(Hb)levels changes,micro inflammatory state including C reactive protein(CRP),interleukin-6 (IL-6),tumor necrosis factor (TNF-a) level changes,TCM syndrome points,treatment efficacy of two groups before and after treatment were compared.Results After treatment,BUN,SCr in observation group were less than those in the control group[(16.08±3.29)mmol/L vs.(21.50±3.68)mmol/L,(330.21±81.96)μmol/L vs.(390.86±84.24)μmol/L],Hb was higher than that in the control group[(107.38±7.37)g/L vs.(98.27±6.36)g/L](P<0.05).CRP,IL-6,TNF-α in observation group were less than those the control group[(5.92±1.14)mg/L vs.(7.26±2.02)mg/L,(24.75±7.12)pg/mL vs.(41.08±9.07)pg/mL,(112.16±20.16)ng/L vs.(134.46±22.16)ng/L],the differences were statistically significant(P<0.05).The score of TCM syndrome in observation group was smaller than that in control group[(5.01±1.21)score vs.(9.36±2.43)score],the difference was statistically significant(P<0.05).The total effective rate of the observation group after therapy was better than the control group[95.00%(38/40) vs.75.00%(30/40)],The difference was statistically significant(P<0.05).Conclusion Angelica containing selenium is well for chronic renal failure,which could effectively improve the micro inflammatory state,and could improve the therapeutic effect.
2.The investigation of oxygen nebulizer use in an upper first-class hosipital
Shurong WANG ; Yilan HUANG ; Yun YE ; Zongyan DAI ; Fei WANG
Chongqing Medicine 2015;(31):4396-4398,4402
Objective To investigate oxygen nebulizer usage among inpatients in an upper first‐class hospital so that this de‐vice can be used by medical personnel and patients properly .Methods Totally 409 patients were investigated about the usage of the device from May 12th to 30th 2014 ,whose outcomes were analyzed by Excel2007 software .Results The total liquid volume of in‐halation on prescription was much larger than the favorable one ,ranging from 4 to 6 mL .And the Oxygen flow ,ranging from 2 .5 to 3 .5 L/min ,was much lower than the normal flow (ranging from 6 to 8 L/min) .In addition ,all patients failed to breathe normally with occasional deep breaths and failed to gargle timely after corticosteroid inhalation .Only 3% patients cleaned their faces after corticosteroid inhalation ,43 .5% patients sit in an upright position ,44 .0% patients kept the nebulizer vertical during treatment .The accuracy rates of mouthpiece position ,the right time to stop nebulizer therapy ,rinsing the nebulizer with sterile or distilled water and allowing to air dry were 95 .1% ,99 .2% ,and 99 .3% ,respectively .Conclusion There are many common patient use nebulizer incorrectlly in the upper first‐class hospital ,and education and guidance by medical personnel should be strengthened .
3.Expression of Pentraxin 3 in Children with Henoch-Sch?enlein Purpura
Fengying WANG ; Lusheng HUANG ; Kang XU ; Linhua YE ; Yun HUANG ; Fei XIAO
Journal of China Medical University 2017;46(2):156-159
Objective Henoch-Sch?nlein purpura(HSP)is a common multisystemic vasculitis in children ,but the exact pathogenesis remains unknown. Pentraxin 3(PTX3),a new kind of inflammatory cytokines,has a strong inflammatory effect,and is involved in occurrence and develop-ment of a variety of autoimmune diseases. The objective of this study is to evaluate the expression of PTX3,interleukin-8(IL-8),tumor necrosis fac-tor-α(TNF-α)and high sensitivity C-reactive protein(hs-CRP)in children with HSP,and explore the clinical significance of PTX3 in HSP devel-opment. Methods Thirty-six children(HSP group)and 17 healthy children(control group)were enrolled in the study. Serum levels of PTX3,IL-8 and TNF-α were detected by enzyme-linked immunosorbent assay. Serum hs-CRP levels were measured by automatic biochemical analyzer. Re-sults The serum levels of PTX3,IL-8,TNF-α,and hs-CRP were up-regulated in HSP group compared with the control group(P<0.05). The levels of PTX3,IL-8,TNF-α,and hs-CRP in patients with joint symptoms,or/and gastrointestinal symptoms,or/and kidney injury were significant-ly higher than those patients without joint symptoms,or/and gastrointestinal symptoms,or/and kidney injury(P<0.05). The expression of PTX3 was positively correlated with the expression of IL-8 and TNF-α(r=0.514,0.833,all P<0.05),but there was no correlation between PTX3 and hs-CRP(r=0.292,P>0.05). The expression of PTX3,IL-8,TNF-α and hs-CRP in HSP patients had no gender difference(all P>0.05). Con-clusion The high expression of PTX3 is related to the degree of inflammation in children with HSP. The up-regulated expression of PTX3 may play an important role in pathogenesis of HSP in children.
4.The Application of Heating Effect in Breeding of Microorganism
Xing-Qiang GAO ; Yun-Hong HUANG ; Fei DAI ; Xue-Qin FU ; Zhong-Er LONG ;
Microbiology 2008;0(10):-
Advances in mechanism and application of the heating effect in breeding of microorganism are reviewed in this paper. Heat produces mutagenesis effect and screening effect. Heating mutagenesis effect is occurred through the substitution of G-C base pair induced by heat, and heating screening effect produces higher forward mutation rate induced by other mutagens.
5.An experimental study of the protective effects of the ultrashort wave diathermy on gentamycin-induced acute renal iujury
Xiang-Yun LI ; Zao-Ming HUANG ; Chang-Fie ZHANG ; Shi-Fei ZHANG ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(07):-
Objective To observe the treatment effects of the ultrashort wave diathermy on gentamycin-in- duced acute renal injury of rats.Methods Eighteen Sgrague-Dawley rats were randomly divided into a normal group(6 rats),a model group(6 rats)and a treatment group(6 rats),the treatment group was treated with ultra- short wave diathermy once a day for a total of 20 days.The observed indexes were NAG,RBP,?_1-MG,?_2-MG in the urine of rats;the SCr,BUN in the blood of rats were also tested and the pathological changes of the renal ob- served.Results The pathological injuries of those in the ultrashort wave treatment group was slighter than the con- trol group;the NAG,RBP,?_1-MG,?_1-MG in the urine and the SCr,BUN in the blood of treatment group were low- er than those of the model group(P
6.Hydroxyapatite modified titanium promotes superior adhension and proliferation of corneal fibroblast in comparison with pure titanium
Xiao, MA ; Zhao-Hui, LI ; Yi-Fei, HUANG ; Yu-Jie, LU ; Le-Yun, WANG ; Jing-Xiang, HUANG
International Eye Science 2007;7(1):6-9
AIM: To determine whether hydroxyapatite modified titanium promotes superior adhension and proliferation of rabbit corneal fibroblast in comparison with pure titanium.METHODS: We used bioactive hydroxyapatite to modify titanium surfaces. Fourth passage fibroblasts of rabbit cornea were seeded on hydroxyapatite modified titanium surfaces, pure titanium and glass surfaces. Cell adhension, proliferation and morphology were detected at 24 hours, 48 hours, and 72hours using a acridine orange stain. Further studies of cell morphology were performed using scanning electron microscopy.RESULT: Ceil counts were significantly greater on hydroxyapatite modified titanium surfaces at each time point(P<0.05).At 24 hours, cell spreading was greater on hydroxyapatite-coated titanium and glass than on the pure titanium. At 72 hours, compared with pure titanium and glass surfaces, the cells on hydroxyapatite modified titanium surfaces had greater spreading area and longer stress fibers.CONCLUSIONS: Hydroxyapatite modified titanium promotes superior adhension and proliferation of rabbit corneal fibroblast in comparison with pure titanium.
7.Polybrominated diphenyl ethers load in human serum of Taiyuan city in 2010.
Fei-fei HUANG ; Jing-guang LI ; Lei ZHANG ; Sheng WEN ; Fei-fei GUO ; Yun-feng ZHAO ; Yong-ning WU
Chinese Journal of Preventive Medicine 2011;45(6):502-505
OBJECTIVEThis study was conducted to characterize polybrominated diphenyl ethers (PBDEs) load in human serum of Taiyuan city in 2010.
METHODSA total of 42 blood samples were collected in Taiyuan city, 2010. Solid-phase extraction (SPE)-gas chromatography-negative chemical ionization-mass spectrometry method was used to measure the concentration of brominated diphenyl ethers (BDE)-17, BDE-28, BDE-47, BDE-66, BDE-99, BDE-100, BDE-153, BDE-154, BDE-183 and BDE-209 and ΣPBDEs was calculated.
RESULTSOf the tested serum samples, ΣPBDEs ranged from 2.09 to 66.74 ng/g of lipid weight (lw), the median and mean was 6.32 ng/g lw and 9.84 ng/g lw, respectively. BDE-209 was the main source of ΣPBDEs and its concentration ranged from no detection to 64.99 ng/g lw, the median was 4.11 ng/g lw, accounting for 67.6% of ΣPBDEs. Second source was BDE-153 and its concentration ranged from 0.29 to 4.92 ng/g lw, the median was 0.62 ng/g lw accounting for 10.1% of ΣPBDEs.
CONCLUSIONThe overall load of PBDEs in the serum of local residents was low in 2010.
Adult ; China ; Cities ; Environmental Monitoring ; Female ; Halogenated Diphenyl Ethers ; blood ; Humans ; Male ; Middle Aged ; Serum ; chemistry ; Young Adult
8.Activation of Nrf2 by 18α-GA affects proliferation of adult neural stem cells
Na YANG ; he Yun ZHAO ; fei Fei HUANG ; wei Qi LIU ; Li LU
Chinese Journal of Pathophysiology 2017;33(10):1738-1745
AIM:To investigate the effect of nuclear factor E2-related factor 2 (Nrf2) activation by 18α-gly-cyrrhetinic acid (18α-GA) on the proliferation and self-renewal of adult neural stem cells (aNSCs), and to explore an ef-fective way of maintaining the viability of aNSCs .METHODS:NSCs were dissociated from subventricular zone of the mice at postnatal days 0, 60, and 300.The expression levels of Nrf2 in the NSCs at various ages were compared .After treatment with 18α-GA, the expression of Nrf2 was examined by real-time PCR and Western blot.shRNA lentiviral vector (LV) car-rying green fluorescent protein (GFP) gene was constructed to knock down Nrf2 expression.The knockdown efficiency in the aNSCs was detected by real-time PCR and Western blot .Subsequently , the aNSCs were divided into DMSO group , 18α-GA group, LV-GFP group and LV-Nrf2-shRNA group.BrdU incorporation assay , Tuj1 staining, CCK-8 assay, Hoechst 33342/PI staining and detection of reactive oxygen species ( ROS) were performed to analyze the proliferation , dif-ferentiation, viability, apoptosis and oxidative stress levels of the NSCs .RESULTS:The mRNA expression level of Nrf2 in adult and aged NSCs was significantly lower than that in newborn NSCs (P<0.01), while the ROS level of aNSCs was significantly higher (P<0.05).After treatment with 18α-GA, the expression level of Nrf2 in the aNSCs was significantly up-regulated as compared with DMSO group ( P<0.01).Increased number of BrdU +and Tuj1 +cells was observed in 18α-GA group, indicating that 18α-GA-treated cells had higher viability (P<0.05).Meanwhile, there were fewer apop-totic cells and lower ROS level in 18α-GA group than those in DMSO group (P<0.05).After knockdown of Nrf2 in aNSCs and then treated with 18α-GA, there were less BrdU +and Tuj1 +cells, as well as the aNSCs with lower viability in LV-Nrf2-shRNA group (P<0.05).Moreover, the ROS level was increased in LV-Nrf2-shRNA group as compared with LV-GFP group (P<0.05).CONCLUSION:Activation of Nrf2 by 18α-GA elevates the antioxidant capacity of aNSCs , thus ameliorating the cell proliferation and differentiation potentials .
9.Complete Genomic Sequence of a Chinese Isolate of Duck Hepatitis Virus
Guang-qing, LIU ; WANG, FEI ; NI, ZHENG ; YUN, TAO ; YU, BIN ; Jiong-gang, HUANG ; Jian-Ping, CHEN
Virologica Sinica 2007;22(5):353-359
The complete genomic sequence of Duck hepatitis virus 1 (DHV-1) ZJ-V isolate was sequenced and determined to be 7 691 nucleotides (nt) in length with a 5'-terminal un-translated region (UTR) of 626 nt and a 3'-terminal UTR of 315 nt (not including the poly(A) tail). One large open reading frame (ORF) was found within the genome (nt 627 to 7 373) coding for a polypeptide of 2 249amino acids. Our data also showed that the poly (A) tail of DHV-1 has at least 22 A's. Sequence comparison revealed significant homology (from 91.9% to 95.7%) between the protein sequences of the virus in the Picornaviridae family, its genome showed some unique characteristics. DHV-1 contains 3copies of the 2A gene and only 1 copy of the 3B gene, and its 3'-NCR is longer than those of other picornaviruses. Phylogenetic analysis to do sequence homology based on the VP1 protein sequences showed that the ZJ-V isolate shares high sequence homology with the reported DHV-1 isolates (from 92.9% to 99.2%), indicating that DHV-1 is genetically stable.
10.Clinical application of maxillary sinus augmentation, bone graft and simultaneously placement of implant with trephine bur.
Yun-fei HUANG ; Lei ZHOU ; Guang-bao SONG
Chinese Journal of Stomatology 2004;39(2):153-154
OBJECTIVETo introduce and evaluate the procedure and the effect of localized management of sinus floor (LMSF), bone graft and simultaneous implant placement with trephine bur in maxillary posterior region.
METHODS24 patients without enough alveolar bone height received LMSF, bone grafting and implants placement were carried out simultaneously.The autogenous bone were harvested by trephine bur in situ. Patients were followed up after 1 week, 1, 3 and 6 month.
RESULTSThere was no implant loose or lost and maxillary antritis. 6 months postoperatively, bone graft reformed to new bone seen in X-ray films, sinus floors were augmented and reached the requirements of dental implants. The implant osseointegrated tightly with new bone which was satisfactory to second-step prosthesis after implant placement of 6 months later.
CONCLUSIONSThe method enlarges the indication of dental implants and avoids operation of harvesting autogenous bone in other site. It is simple and valuable to clinical application.
Adult ; Alveolar Ridge Augmentation ; methods ; Bone Transplantation ; Dental Implantation, Endosseous ; methods ; Dental Implants ; Female ; Humans ; Male ; Maxillary Sinus ; surgery ; Middle Aged