Objective:In the Social background that the increasingly serious problem of ageing population. Tele-home Health Management System(THMS)is constructed for elderly people founded on Android platform.Methods: On the basis of analyzing system requirements, establishing system firmaments, utilizing Client/server structure.Results: Building an Android-based health management system for old people staying at home to know their health condition and get health guidance on time and for the doctors to get access to patients' medical history.Conclusion: Android elderly home-based health management system has broad application prospects for resolving the digital medical problems in health management provides ideas and solutions.

Objective To isolate and purify the membrane protein of SeAx cells, which was derived from cutaneous T-cell lymphoma cell line (CTCL), and to prepare polyclonal antibodies against membrane proteins. CTCL antigens were screened with SEREX method from the cDNA expression library of SeAx cells. Methods SeAx cells were cultured to 2?1010 and membrane proteins were purified by means of differential centrifugation after homogenate was made, and organelle protein specific antibodies were used to perform Western blot. Rabbit was immunized 4 times by the membrane proteins, and the serum antibody titer was measured by ELISA after each immunization. The specific interactions between antibodies in serum and SeAx proteins were detected by Western blotting. E. coli were initially transfected by recombinant phages, and the recombinant proteins expressed were transferred onto the nitrocellulose membranes, which were then again reacted with diluted serum from the immunized rabbit. Positive clones were subcloned and the nucleotide sequence of the cDNA inserts was determined. Finally, cDNA sequence analysis via database searching was performed to define the gene. Results Membrane proteins of SeAx cells were purified by differential centrifugation and a 1?10-5 titer of antibody was obtained after 4 times of immunization. 1?106 phage clones were screened, 25 of which were positive. Nucleotide sequencing and database searching showed that 4 cDNA inserts were cell membrane proteins, respectively as integrin alpha 4,ligatin, matrix metalloproteinase 24 and MHC-I molecule HLA-A. Conclusion Membrane proteins of CTCL and SeAx cells, are purified and polyclonal antibodies against SeAx cell membrane proteins are successfully prepared. The 4 genes of membrane proteins are identified by means of SEREX from SeAx cDNA library, expressed by phages. These genic candidates are able to induce systemic humoral immune reactions and might therefore be promising targets for immunotherapeutic interventions of CTCL.

Calcium overload has been discovered in the ischemia and reperfusion injury of the heart recently, but there were less reports about changes in myocardial Ca distribution under this same condition by electronmicroscopy. Using the potassium-pyroantimonate method and X-ray microanalysis, we studied cytochemical calcium localization in the rabbit's myocardium at various time periods of ischmia with or without subsequent reperfusion. At the same time, we had the morphometric estimation of Ca granule volume density in the mitochondria by TAS(texture analysis system) and evaluated Danshen's protection for ischemia and-reperfusion injury. The results showed that mitochondrial Ca granule volume density increased significantly with the development of ischmia and post-ischemic reperfusion, especially during reperfusion following 40min of ischemia, however following 60min of ischemia, Ca granule volume density decreased than that observed following 40 min of ischemia. This meant that the limit time point for reversible and irreversible injury could be in the range of 40～60min of ischemia. Danshen pretreatment to the rabbits reduced Ca granule volume density significantly. This demostruted that Danshen acted as a calcium antagonist and contributed an outstanding protection to the myocardial calcium overload.

Because of lots of ethical problems,military medical triage has been unaccepted by general doctors.But it's adopted by American Army in front of facts of the war.The main principles of the military medical triage are justice and efficiency.They practice the military medical triage through tow steps.First,all patients are assigned into urgent,immediate ,delayed,minimal or ambulatory and expectant status in decreasing order of medical urgency.Second,choosing the model of triage depends on nonaustere,austere or extreme conditions.The ethical issues of American military medical triage focus on four aspects - how to deal with minimally injured patient treatment first,how to treat expectants,how to care for noncombatant casualties and who decide the model of triage.

Objective To investigate the effect of Tianma Gouteng Decoction and Captopril on blood pressure and its mechanism in the senile patients with essential hypertension(EH) in the pattern of ascendant liver yang.Methods Eighty cases with EH were ran- domly divided into two groups,with 40 cases in each group.Treatment group was given Tianma Gouteng Decoction and Captopril and the control group was given only Captopril.The treatment was lasted for 4 weeks.Observed were the changes in blood pressure and serum hs-CRP,TNF-?and IL-6.Results There was significant difference in blood pressure change and improvement of clinical symptoms after treatment between the two groups.The level of serum hs-CRP,TNF-?and IL-6 decreased significantly in the treat- ment group.Conclusion Tianma Gouteng Decoction and captopril can decrease blood pressure and serum hs-CRP,TNF-?and IL-6, and the effect was superior to captopril simply.

The rupture of vulnerable carotid atherosclemtic plaques and distal embolization of atherornatous debris are the most important pathogenesis of atherosclerotic stroke.The serolog- ic(and plasma)markers associated with vulnerable plaques will change significantly in this process,and show their different meanings.The studies in this field are in its infancy and still need to be further validated,This article reviews the advances in research on the correlation of these biomakers with vulnerable plaque and ischemic cerebrovascular disease,and briefly intro- duces the clinical application prospect of some of the biomakers.

OBJECTIVE:To systematically review the efficacy and safety of glipiaide versus repaglinide in treatment of type 2 diabetes and provide evidence-based reference for the clinical treatment. METHODS:PubMed,EMBase,Medline,Cochrane Li-brary,CJFD,VIP and WanFang database were retrieved to collect the randomized controlled trials(RCT)of the efficacy and safety of glipizide(test group)versus repaglinide(control group)in the treatment of type 2 diabetes. After the quality evaluation and in-formation collection of clinical studies with inclusion criteria,Rev Man 5.2 software was conducted for Meta-analysis. RESULTS:There were totally 10 RCTs,involving 1 022 patients. Meta-analysis results showed that the HbA1c levels [MD=0.43,95%CI (0.20,0.65),P<0.001],2 h postprandial plasma glucose levels [MD=0.49,95% CI(0.04,0.94),P=0.03] and hypoglycemia inci-dence [OR=2.99,95%CI(1.83,4.88),P<0.001] in test group were significantly higher than control group. There was no signifi-cant difference in the fasting plasma glucose level in 2 groups[MD=-0.14,95%CI(-0.44,0.16),P=0.35]. CONCLUSIONS:Re-paglinide has better efficacy and safety than glipizide in the treatment of type 2 diabetes. Limited by the methodological quality and sample amount of included studies,it remains to be verified by RCT with large sample,strict design and long-term follow-up.

Objective To investigate nursing cooperation during gastric coronary vein embolization procedure with NBCA glue. Methods Perioperative nuusing experience in 36 hepatic cirrhosis patients complicated with esophagus-gastric fundus vein varicose rupture and hemorrhea who had undergone gastric coronary vein embolization procedure was retrospectively summarized. Results Operations in all 36 cases had been successful,with no severe complication due to inadequate nursing cooperation. Conclusions Gastric coronary vein embolization operation utilizing NBCA glue is a safe and effective method of hemostasis,skilled and elaborative nursing cooperation is an important component ensuring a smooth and effective operation.

Objective: The total triptolides in lactone leaves are generally extracted by decocting with chloroform,which is known to be a poisonous substance and pollutes the environment.The authors aimed to evaluate the use of macroporous resin in the extraction process.Methods: We measured the total triptolides by spectrophotometry and investigated the technology of extracting the total triptolides with macroporous resin,including determination of the concentrations of decoction and alcohol in the alcohol precipitation as well as the consumption of resin.Results: The total triptolides were enriched with the combination method of decoction by alcohol precipitation with macroporous resin.The ratio of concentration medicine to decoction was 1 ∶ 2,and that of the volume proportion of alcohol to decoction in the precipitation of alcohol was 2 ∶ 1.The appropriate amount of resin was 15%.Conclusion: Macroporous resin can be used to extract the total triptolides.

Objective To explore the distribution and characteristic of class 1 integrons among Shiga toxin-producing E.coli(STEC) in China,and to elucidate the type of cassettes.Methods Antibiotics susceptibility was tested by the disk diffusion method,class 1 integron was detected by PCR assay and PCR products were sequenced and analyzed in eight isolates.Results Four isolates were multiple-drug resistant whose antibiogram were ampicillin,tetracycline,erythromycin,sulfamethoxazole/trimethoprim and ciprofloxacin,but two isolates conferred resistance of streptomycin and spectinomycin simultaneously.Only the two isolates carried class 1 integrons ranging in 1 000 bp which located on the plasmid of 7 800 bp.The sequenced PCR product demonstrated that the 1 000 bp integron harbored aad A1 gene cassettes conferred the resistance of streptomycin and spectinomycin;and linked with sul 1 and qacE?1 gene conferred the resistance of sulfamethoxazole and quaternary disinfectants.Conclusion The class 1 integrons exists among STEC isolates in China and determines the resistant antibiotics.