1.Determination of Carbon Tetrachloride in Shampoo by Solid Phase Microextraction-Gas Chromatography/Mass Spectrometry With Carbon Basal Body
Ruibin FANG ; Jian WANG ;
Journal of Environment and Health 1993;0(01):-
After headspace sampling with apparatus of carbon basal body solid phase microextraction the collected samples were directly measured by the gas chromatography/mass spectrometry (GC/MS) The qualitative analysis of carbon tetrachloride (CCl 4) in a certain kind of shampoo was carried out by searching the data base of mass spectrums and using standard addition method Afterward,under the same conditions of sampling and GC/MS analysis metioned above,the quantitative analysis of CCl 4 in shampoo samples was carried out by standard adding method The correlation coffecient r of linear relationship,detection limit and relative standard deviation of this method was 0 998,1 3?10 -8 (V/V),5%~9% respectively
2.Survey of diabetic retinopathy of the diabetic population over 45 years old
Fang, WANG ; Chun-fang, WANG ; Jian-lin, YAN
Chinese Journal of Experimental Ophthalmology 2013;31(8):783-787
Background Diabetiretinopathy(DR) ione of the leading causeof blindness.To understand the risk factorof Dpathogenesiiimportanfothe prevention and treatmenof DR.Objective The purpose of thistudy wato survey the prevalence and relevanfactorof Damong diabetipatientaged ove45 yearold.Methodcross-sectional study wadesigned.physical examination waperformed on 7300 subjectfrom July,2010 to March,2011 athe FirsHospital of Shanxi Medical University,and 5012 of them received non-mydriatidigital funduphotography.Type 2 diabetimellituwadetermined in 574 subjects,and 480 casethaconformed to the criteriof the currenstudy were recruited.questionnaire survey wacarried ouin the 480 patients,and the prevalence of diabeteand Dwacalculated fothe analysiof the causative factorof DR.ResultGradable funduphotographwere available in 480 subjects,and 98 of them presented with Dcomplication aprevalence rate of 19.8%.The morbidity rateof Dwere 14.3%,19.4%,23.7% and 21.5% in the 45-55 yeargroup,>55-65 yeargroup,>65-75 yeargroup and >75 yeargroup,respectively,withousignificandifference among them (x2 =3.824,P=0.281).The incidence rateof Dwere 11.8%,13.2%,15.4%,27.0% and 62.5% in patientwith disease courseof lesthan 5 years,5-10 years,> 10-15 years,> 15-20 yearand ove20 years,respectively,with significandifference among differendisease duration(x2 =57.518,P =0.000).No significandifference waseen in the prevalence of Damong groupwith body weighindiceof <18.5,18.5-22.9,23.0-24.9 and ≥25.0 (16.7%,23.3%,16.8%,19.7%) (x2 =2.099,P =0.718) and differentreatmenmethod,such aoral antidiabetidrugs,insulin injection ocombination treatmen(18.8%,25.9%,19.8%,respectively) (x2 =1.477,P=0.478).The questionnaire survey showed close relevancy between gender,disease duration,fasting glucose concentration,family history of diabetes,and whethefunduexamination waperformed (P =0.025,0.000,0.001,0.003,0.039).ConclusionThe prevalence rate of Dwaassociated with the awarenesfodiabeteand treatmenmodes.Gender,disease duration,family history of diabetes,fasting glucose concentration and submission to eye examination are the independenrisk factorof DR.
3.A case of Rabson-Mendenhall syndrome.
Li MA ; Jian WANG ; Yuan-xun FANG
Chinese Journal of Pediatrics 2013;51(7):545-547
Acanthosis Nigricans
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diagnosis
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genetics
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pathology
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Adolescent
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Antigens, CD
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genetics
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Biomarkers
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blood
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DNA Mutational Analysis
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Donohue Syndrome
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diagnosis
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genetics
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pathology
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Humans
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Insulin Resistance
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genetics
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Male
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Mutation
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genetics
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Polymerase Chain Reaction
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Receptor, Insulin
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genetics
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Sequence Analysis, DNA
5.Advancement in chemotherapy and molecular targeted therapy of advanced gastric cancers
Fang WANG ; Jiang LIU ; Jian LUO
Journal of Chinese Physician 2016;18(1):18-21,25
Chemotherapy is still the main treatment in advanced gastric cancer by now,but no standard approach has been adopted.Chemotherapy can prolong median overall survival and improve quality of life compared to the best supportive care alone.Recent years,with the appearance of various new agents,chemotherapy remains continuous improvement.As a result,response rate and overall survival of advanced gastric cancers were further improved.Meanwhile,with a greater understanding of the molecular biology of gastric cancer,molecular biological targeted therapies that target human epidermal growth factor receptor 2 (HER-2),endothelial growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) become the focus of the comprehensive treatment.Chemotherapy combined targeted therapy have shown great benefit and perspective in treatment of advanced gastric cancer,but their usage should be further modified and optimized in clinical trials and practices.
6.Urinary Tract Infection and Drug Resistance of Pathogens:Investigation and Analysis
Jian WANG ; Lingmei FANG ; Yiping LI
Chinese Journal of Nosocomiology 1994;0(01):-
OBJECTIVE To investigate the distribution and drug resistance of pathogens in urinary system infection.METHODS Collected from patients urine specimens of our hospital during Jan 2004 Dec 2008 were cultured,detected and studied drug resistance.RESULTS Among these pathogens,Escherichia coli rated the top one(44%),followed by coagulase negative Staphylococcus,fungi,Enterococcus,Klebsiella pneumoniae,Proteus and Pseudomonas aeruginosa.The Enterobacteriaceae were sensitive to imipenem(100%) and the Enterococcues and Staphytlococcus were sensitive to vancomycin(100%).But they all showed higher drug resistance to other antibiotics.CONCLUSIONS E.coli from urinary tract infection is the most common pathogen.In the treatment of urinary tract infection the commonly used drugs such as gentamicin,ciprofloxacin,etc,have produced a higher resistance.Clinician should pay attention to the changes in their drug resistance and use correctly.
8.Report of a case with Paget disease with bilateral progressive hearing loss as the first symptom.
Li MENG ; Hui-Fang ZHOU ; Jian-Ling WANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2009;44(7):602-603
Aged
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Female
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Hearing Loss, Bilateral
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diagnosis
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etiology
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Humans
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Osteitis Deformans
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complications
;
diagnosis
9.Analysis of risk factors of the severity of post-ERCP hemorrhage in the patients with choledocholithiasis
Jian FANG ; Yinchu ZHAN ; Renya JIANG ; Zhengfei WANG
Chinese Journal of Primary Medicine and Pharmacy 2017;24(2):217-220
Objective To study the risk factors related to the severity of post-ERCP hemorrhage in the patients with choledocholithiasis.Methods Clinical data of 56 patients with choledocholithiasis and post -ERCP hemorrhage from January 2008 to August 2015 were analyzed.Made the occurrence of severe hemorrhage as dependent variable,supposed 18 factors in patients and procedure related aspects as possible covariates,analyzed with univariate and binary logistic regression.Results Severe hemorrhage was occurred in 1 1 patients after ERCP.Among patients related factors,female,history of oral corticosteriod,level of bilirubin,size of stone,periampullary diverticula, prolonged time of bleeding-cruor,acute cholangitis,acute pancreatitis and course of disease were proved to be signifi-cant risk factors by univariate analysis(P<0.01 ).Periampullary diverticula,prolonged time of bleeding-cruor and acute cholangitis were independent risk factors in further multivariate analysis(P=0.023,0.036,0.045).Among procedure related factors,EPBD(P<0.01),EST(P<0.01),residual biliary stones(P=0.029)were proved to be significant risk factors by univariate analysis,and EPBD was found as independent risk factor(P=0.029)by multiva-riate analysis.Conclusion Appearance of periampullary diverticula,prolonged time of bleeding -cruor and acute cholangitis were considered as risk factors related to severity of post-ERCP hemorrhage in patients with choledocholi-thiasis.Moreover,EPBD was also an independent risk factor which might aggravate severity of post-ERCP hemor-rhage.
10.Analysis on association between polymorphism of CD14 and IL-8 gene and susceptibility of necrotizing enterocolitis
Jiayi TIAN ; Tong ZHU ; Jian WANG ; Mingli FANG ; Chaoying YAN
Journal of Jilin University(Medicine Edition) 2016;42(5):958-962
Objective:To investigate the relationship between the gene polymorphism cluster of differentiation 14 (CD14)-159C/T (rs2569190),and interleukin-8 (IL-8)-251A/ T (rs4073)and the susceptibility of necrotizing enterocolitis (NEC),to clarify the influencing factors of susceptibility of NEC and to provide genetics theory basis for the research on the pathogenesis of NEC. Methods:Total 28 newborns with NEC and 41 newborns without NEC were selected.The amplification of peripheral blood DNA was conducted by PCR.The genotypic and allelic frequencies of CD14-159C/T and IL-8-251A/T of the patients were detected by Sanger DNA sequencing method. The relationship between them and the susceptibility of NEC was studied.Results:The distribution of genotypic frequencies of CD14-159C/T and IL-8-251A/T was consistent with Hardy-Weinberg equilibrium (P >0.05).There were no significant differences of the allelic and genotypic frequencies of CD14-159C/T,or genotypic frequencies of IL-8-251A/T between two groups (P >0.05).While in NEC group,the T allelic frequency of IL-8-251A/T site was higher than that in control group (χ2 = 4.184, P = 0.041, OR = 2.14, 95% CI: 1.03 - 4.46 ). Conclusion:The polymorphism of CD14-159C/T is irrelevant to the pathogeny of NEC,but T allelic frequency of IL-8-251A/T site might be related to the susceptibility of NEC.So T allele in IL-8-251A/T may be one of the danger factors of NEC.