1.Mechanismof reproductive toxicity of sub-chronic exposure to nickel oxide nanoparticles in male SD rats
fan xingjun ; gu hongmei ; yang yong
China Occupational Medicine 2022;49(2):140-147
Objective To investigate the effects of sub-chronic exposure of nickel oxide nanoparticles (Nano NiO) on
endocrine function of male SD rats,and to analyze the toxicity and mechanism of Nano NiO on testicular cells. Methods The
specific pathogens free male SD rats were randomly divided into five groups with ten rats in each group. Rats in low-,medium
and
high-dose groups were given Nano NiO suspension with the mass concentration of 0.16,0.80 and 4.00 g/L,respectively;
rats in blank control group were given equal volume of 0.9% sodium chloride solution;rats in positive control group were given
micron nickel oxide suspension with the mass concentration of 4.00 g/L. Drip every three days for nine weeks. After the Nano
NiO exposure,atomic fluorescence spectrometry was used to determine the levels of nickel in the blood and testicular tissue. The
enzyme-linked immunosorbent assay was used to detect serum level of sex hormone. The ploidy ratio,cell cycle and apoptosis
rate of testicular cells were analyzed by flow cytometry. Western blotting was used to detect the relative expression of apoptosis
related
proteins in the testis. Results The level of nickel in blood and testicular tissue of rats in positive control group and the
three doses groups were higher than that of blank control group(all P<0.05). The level of nickel in blood and testicular tissue of
rats in the medium-dose and high-dose groups were higher than that in the positive control group(all P<0.05). There was a
positive correlation between the level of nickel in blood and testicular tissue(P<0.01). The serum levels of testosterone,follicle
stimulating
hormone(FSH)and luteinizing hormone(LH)in the medium- and high- dose groups were lower than that in blank
control group(all P<0.05). However,there was no significant difference in serum gonadotropin-releasing hormone among all
groups(P>0.05). Compared with the blank control group,the proportion of haploid and diploid cells and the ratio of cells in G0/
G1 and S phase decreased in the medium- and high-dose groups(all P<0.05),the tetraploid cell ratio,G2/M cell ratio and
early apoptotic rate of testicular cells increased(all P<0.05). Compared with the blank control group,the relative expression of
B-cell lymphoma-2(BCL-2)protein and the ratio of BCL-2/BCL-2-related X protein(BAX)in testicular cells of rats decreased
in the medium- and high-dose groups(all P<0.05),the relative expression of BAX and caspase-3 protein were increased(all P<
0.05). Compared with the positive control group,the level of nickel in blood and testicular tissue of rats was increased in the
high-dose group(all P<0.05),the ratio of haploid cells and the ratio of testicular cells at G0/G1,S phase and BCL-2 /BAX ratio
in testicular tissue decreased(all P<0.05),the tetraploid ratio,G2/M phase ratio,early apoptotic rate and total apoptotic rate
of testicular cells increased(all P<0.05). Conclusion Exposure to Nano NiO could inhibit the secretion of FSH,LH and
testosterone in male rats. Nano NiO can cross the blood-testosterone barrier,interfere with the proliferation of testicular cells,
induce apoptosis of testicular cells through the mitochondrial apoptosis pathway,inhibit the formation of haploid sperm cells,
resulting in disorders of spermatogenesis.
2.Clinical value of serum IL-2,IL-6,IL-10,ChE and HA changes in patients with chronic hepatitis B and liver cirrhosis
Song XU ; Xingjun GU ; Guoqi YANG ; Xiaoxia WANG ; Dachun FAN
International Journal of Laboratory Medicine 2014;(15):1986-1988
Objective To study the changes and clinical significance of serum interleukin-2 (IL-2) ,interleukin-6 (IL-6) ,interleu-kin-10 (IL-10) ,cholinesterase (ChE) and hyaluronic acid (HA) in the patients with chronic hepatitis B (CHB) and liver cirrhosis (LC) .Methods The serum concentrations of IL-2 ,IL-6 ,IL-10 ,ChE and HA were detected in 92 cases of CHB(CHB group) ,63 cases of LC (group LC) and 46 cases of healthy populations (control group) .Results The serum concentrations of IL-2 ,IL-6 and HA in the CHB group and the LC group were higher than those in the control group ,while the serum concentrations of IL-10 and ChE were lower than those in the control group ,the differences had statistical significance (P<0 .05) .The serum concentrations of IL-2 ,IL-6 and HA in the patients with HBV DNA copy number of 6 .0-6 .9 log10 copy/mL were higher than those with the copy number of 3 .0-4 .9 log10 copy/mL and 5 .0 -5 .9 log10 copy/mL ,while the serum concentrations of IL-10 and ChE were lower than those with HBV DNA copy number of 3 .0-4 .9 log10 copy/mL and 5 .0-5 .9 log10 copy/mL ,the differences had statistical significance(P<0 .05) .The serum concentrations of IL-2 ,IL-6 and HA in the LC patients with the Child-Pugh grade C were higher than those with the Child-Pugh grade A and B ,while serum concentrations of IL-10 and ChE were lower than those with the Child-Pugh grade A and B ,the differences were statistically significant (P<0 .05) .Conclusion The simultaneous detection of serum IL-2 ,IL-6 ,IL-10 ,ChE and HA concentration changes can provide certain clinical reference value for the evaluation of severity and prognosis in the patients with CHB and LC .
3.Calibrating the working standard for rhG-CSF
Qian JIA ; Xingjun ZHOU ; Xuehong TIAN ; Yuling FAN ; Xiaobo QI ; Zhihua ZHANG
Chinese Journal of Biochemical Pharmaceutics 2001;22(2):81-84
Purpose Both the working standard and corresponding sample(GRAN75)were first calibrated by WHO international standard for G-CSF.Methods MTT method by NFS-60 cells was used. The results were calculated by (4,4)method.Results Three batchs working standards were prepared,two batchs were freeze-dry and the prescription was same as WHO international standard for G-CSF,one batch was injection and the prescription was same as corresponding sample(GRAN75).The biological potency and the FL% of average potency were 3.062×106 、4.276×106IU/ampoule、1.635×107IU/ml and 5.529%、4.291%、4.244% for working standard, and 1.880×107IU/ml and 5.175% for corresponding sample,respectively.Conclusion The working standard which calibrated could be used as working standard in the measurement of rhG-CSF biological activity.
4.Study on determination method of the rhG-CSF biological activity
Qian JIA ; Xingjun ZHOU ; Xuehong TIAN ; Yuling FAN ; Xiaobo QI ; Zhihua ZHANG
Chinese Journal of Biochemical Pharmaceutics 2001;22(1):25-26
Purpose The determination method of rhG-CSF bio logical activity by NFS-60 cells was studied in accordance with WHO internation al standard for G-CSF.Methods MTT method was adopted.R esults The chomosone number of NFS-60 cell was 39, about 1×105 cells/ml to 1×107 cells/ml,the dying color showed gradient when the NFS-60 c ells was dyed by MTT,the method(4,4) was adopted in the determination of rhG-CS F biological activity,the average FL% of potency was 13.560% for single exprimen t,and the CV of inter-assay and intra-assay was lower than 10% and 10.109%,respectively.Conclusion method(4,4) can be us ed in the determination of rhG-CSF biological activity,and the results can guid e the study and the manufacture of rhG-CSF effectively.