Dysfunction of microRNA (miRNA) is associated with occurrence and development of tumor.MiRNA are very stable in blood serum,significantly tumor-related and tissue-specific.The detection of serum miRNA is convenient,little-invasive and fast,and then serum miRNA can be used as a new biomarker for tumor diagnosis.

The G protein-coupled receptor family C,member 5,group A (GPRC5A) gene is known as retinoic acid-induced gene,which is mainly distributed in lung tissue.The expression of GPRC5A in lung cancer is significantly decreased compared with normal lung.GPRC5A leads to lung cancer through knockout mice,which is proven to be a suppressor gene of lung cancer.GPRC5A may be a novel biomarker for the diagnosis of lung cancer and a new target for the treatment of lung cancer.

Objective:To grasp the macro trend of the U.S.military medical advances through the knowledge mapping of 2 966 records in journal Military Medicine.Methods:CiteSpace Ⅱ running on Java platform was used.Results:Military medicine was closely related to federal medicine in the U.S.,and this relation was definite and clear.The missions of the U.S.military medical research institutions were different and were not obviously overlapping.A number of national agencies and military agencies as well as some experts formed the solid foundation for the development of military medicine. Key literature of military medicine could be visually displayed. The U.S. military medicine focused on combat casualties, PTSD, veteran affairs, dental emergencies and health promotion.Conclusion:Novelty, necessity and applicability of the knowledge mapping analysis method are displayed in the field of military medicine.

Objective To explore clinical advantages of N‐terminal pro‐B‐brain natriuretic peptide(NT‐proBNP) in early diagno‐sis and therapeutic effect monitoring of acute coronary syndrome(ACS) .Methods A total of 97 cases of patients with chest pain and obvious discomfort who treated with coronary angiography in this hospital from February to December 2014 were selected .A‐mong these patients ,34 cases with non‐ACS were enrolled in the control group ,while other 63 cases with ACS were divided into the ST‐segment elevation myocardial infarction(STEM ) group ,non‐ST‐segment elevation myocardial infarction(NSTEM ) group and unstable angina pectoris(UA) group .The glycemia ,lipemia ,blood pressure and other indicators were observed .Results The levels of fasting blood‐glucose were within the normal reference interval in all groups ,while levels of lipemia ,plasma NT‐proBNP and blood pressure were higher than the upper limits of normal reference intervals ,and plasma levels of NT‐proBNP were higher than standard of good prognosis(NT‐proBNP<200 pg/mL) .There was statistically significant difference in plasma levels of NT‐proB‐NP among these groups(P<0 .05) ,while no statistically significant differences were found in other indicators among these groups (P>0 .05) .The levels of lipemia and plasma NT‐proBNP and blood pressure were obviously decreased in patients with ACS at each treatment stage .Conclusion NT‐proBNP as a kind of myocardial marker has high accuracy in early diagnosis of ACS ,which could be applied in therapeutic effect monitoring and provide guidance for reasonable selection of treatment strategy .

Introduction: The per capita consumption of chicken meat and related products has increased steadily in Malaysia, as it is affordable and can be consumed without religious constraints. There is concern for the widespread use of phosphate additives in processed meats, which may lead to hyperphosphatemia, especially among chronic kidney disease (CKD) patients. The objective of this study was to determine and compare the phosphorus content in raw chicken breast meat (RCBM) and selected processed chicken meat products. Methods: Samples of RCBM, chicken frankfurters, chicken patties and chicken nuggets of different brands were studied. The phosphorus content of the samples were determined via the dry ashing method and a Perkin-Elmer 5300DV inductively coupled plasma-optical emission spectrometer (ICP-OES). Results: The mean phosphorus content in RCBM was 209.15±3.13 mg per 100 g. Chicken nuggets contained the highest phosphorus content, followed by RCBM, chicken patties and chicken frankfurters. Compared to the RCBM, the mean phosphorus content of chicken frankfurters and chicken patties were 21.42% and 4.81% respectively lower, whilst that of chicken nuggets was 1.74% higher. The same type of chicken meat products from different brands also differed significantly in their phosphorus content. Conclusion: There were significant differences in the phosphorus content among different types of chicken meat products, and among the same chicken meat products from different brands. Caution should be exercised, especially by CKD patients, in consuming processed chicken meat products due to the risk posed to them by phosphorus content.

OBJECTIVE:To establish HPLC method for the determination of hesperidin in xiaoermoji tablets.METHODS:The determination was performed on Spherisorb C 18 ,the mobile phase consisted of methanol-glacial acetic acid-water(25∶4∶71),the detection wavelength was283nm,the flow rate was2.0ml/min,the column temperature was50℃,the sensitivity was0.16AUFS and the sample size was20?l.RESULTS:Hesperidin has a good linear relation with peak area score when its sample size was within a range of0.024?g～1.2?g(r=0.9999),the average recovery was99.1%(RSD=0.8%).CONCLUSION:The method is simple,fast,sensitive and accurate,and it can be served as a basis for the quality control of xiaoermoji tablets.

Orbital cellulitis is an infection of the orbital soft tissue that could possibly lead to serious complications if treatment is delayed. It is important to differentiate this condition from a less life-threatening condition like preseptal cellulitis as orbital cellulitis would require a more aggressive treatment than the former condition. The present case discusses about a patient who was diagnosed with orbital cellulitis and developed complications from the disease. The delayed administration of the appropriate antibiotic subsequently led to the patient’s death. The delay was because of the reason that the patient belonged to the immunocompromised group who harbour different causative organism than the normal population.

Objective To study the relationship of EGFR gene mutation and clinical efficacy of patients with terminal NSCLC treated with gefitinib from Zhoushan.Methods 45 patients with terminal NSCLC were divided into the mutation group and normal group according to EGFR mutation.And all patients were received gefitinib treatment. Clinical efficacy was observed.Results The objective response rate and PFS of the mutation group were 76.92%, (232.8 ±24.3)d;The objective response rate and PFS of the normal group were 31.25%,(156.7 ±26.5)d;The objective response rate of the mutation group was significantly higher than that of the normal group(χ2 =7.810,P <0.05),and the PFS of the mutation group was also significantly higher than that of the normal group(t =5.665,P <0.05).Conclusion EGFR gene mutation patients with terminal NSCLC treated with gefitinib has higher clinical effi-cacy.

Endometrial cancer is one of the malignant tumors of female reproductive system. The prognosis of advanced endometrial cancer is poor. How to improve the overall survival rate and improve the prognosis of patients with advanced endometrial cancer is still one of the clinical problems. In recent years, the research on tumor gene changes is increasing gradually. Epigenetics is the study of genetic changes in gene expression without changing the nucleotide sequence of genes. Epigenetics includes many phenomena, such as DNA methylation, microRNA expression and gene silencing, chromatin remodeling. These changes may be important factors in the occurrence and development of tumors, and may become new therapeutic targets for tumors. With the study on epigenetics of endometrial cancer in the future, new therapeutic targets may be found. Meanwhile, those study may provide new ideas for targeted therapy of endometrial cancer.