No abstract available.
Enzyme-Linked Immunosorbent Assay*

No abstract available.
Enzyme-Linked Immunosorbent Assay*

No abstract available.
Enzyme-Linked Immunosorbent Assay*

No abstract available.
Enzyme-Linked Immunosorbent Assay* ; Korea*

No abstract available.
Autopsy* ; Enzyme-Linked Immunosorbent Assay*

BACKGROUND: The aim of our study was to optimize and establish erythropoietin (EPO) enzyme linked immunosorbent assay (ELISA) system. METHODS: We prepared several monoclonal and polyclonal antibodies specific to human-EPO. The best combinations of antibodies for coating and detecting antibodies were selected for the establishment of ELISA. We tested several methods such as a competitive EIA and a sandwich ELISA. RESULTS: The best sandwich ELISA was optimized compared to competitive EIA when purified polyclonal antibody (PoAb) was used as a coating antibody and biotinylated PoAb as a detecting antibody. This sandwich ELISA easily detected EPO when PoAb pairs were used compared to the ELISA using monoclonal antibody and PoAb. There were no significant differences between the effects of various blocking solutions on the performance of sandwich ELISA using biotinylated antibody. The ELISA system using PBST containing 3% BSA as a blocking solution can sensitively detect EPO (10 mU/mL) in a broad range of EPO concentrations (10-2,000 mU/mL) and there were cross-reactions with other cytokines). CONCLUSIONS: EPO can be easily determined by using biotinylated PoAb as a detecting antibody and another PoAb as a coating antibody.
Antibodies ; Enzyme-Linked Immunosorbent Assay* ; Erythropoietin*

In the year 2003, for Hantaan virus, a serological surveillance ELISA method was performed on 155 serum samples collected from the workers of Haiphong port.26/155 samples were positive or in suspect with rodent sera. The samples submitted to 2nd examen by indirect fluoresceine immuno-assay showed that 3/26 samples were positive 3 positive samples were undertaken further studies by WB technique with specific SEO antigenes ,which confirmed anti-hantaan virus (IgG) and nuclecapsid protein (NP) of51 kD weigt. Results showed that Hantaan virus was found among Haiphong port workers
Hantavirus ; Serum ; Enzyme-Linked Immunosorbent Assay

Immunology plays an important role in prevention, discovery, diagnosis and treatment. There are 4 major immunological techniques including ELISA (enzyme linked immuno-sorbent assay), ICA (immuno-chromatography assay), GLORIA (gold labeled optical read rapid immunoassay) and MCIA (membrane capture immunoassay). These techniques with the simple test devices such as test paper, test stick, test cassette provided the rapid results. The immunological techniques have been applying in emergency tests (myocardial infection, embolism, thrombosis, and occlusion), infection viral infection, allergy, cancer and drug abuse
Immunology ; Immunoassay ; Enzyme-Linked Immunosorbent Assay

The authors studied on 527 infertile women. All patients were done: clinical examination, spermatogram analysis, hysterosalpingography and quantitative determination of 5 hormones LH, FSH, PRL, E2, A4P. The classification of hormonal causes of female infertility is following: - Primary ovarian failure (POF): 18 cases- 3.42%. - Hyperprolactinemia: 12 cases 2.28%.- Anovulation: 241 cases 45.92%. - Ovulation cycle: 182 cases 34.54%. - Uncomprehensible: 73 cases 13.85%.
Infertility ; Infertility, Female ; Enzyme-Linked Immunosorbent Assay

On 138 serum samples taken in the first 7 days of acute syndrome of brain, by MAC-ELISA technique 95 samples were determined as positive, 68.84% was positive. 43 samples taken in the first 7 days of the disease there was no detected antivirus IgM antibody but this could not concluded as negative, but may be a late antibody response or may be another cause of acute brain syndrome. For assuring the accuracy of the diagnosis, the samples taken in the first 7 days of the disease must be taken again after 7 day to verify the results.
Encephalitis, Japanese ; Diagnosis ; Enzyme-Linked Immunosorbent Assay