Adolescent ; Adult ; Aged ; Aged, 80 and over ; Apolipoproteins E ; blood ; genetics ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; Polymorphism, Genetic ; Venous Thromboembolism ; blood ; genetics ; Young Adult ; alpha-2-Antiplasmin ; analysis ; genetics

Cardiomyopathy, Hypertrophic ; complications ; Down Syndrome ; complications ; Humans ; Infant, Newborn ; Male

Adult ; Exons ; Female ; Gastrointestinal Stromal Tumors ; genetics ; metabolism ; pathology ; Gene Deletion ; Humans ; Proto-Oncogene Proteins c-kit ; genetics ; metabolism

OBJECTIVETo investigate the epidemiological characteristics of human papillomavirus (HPV) infection in men attending a sexually transmitted diseases (STD) clinic in Hangzhou area.

METHODSMale subjects (n=375) aged 18-70 years, attending the STD clinic were recruited. Urethral swabs were assessed for HPV DNA using polymerase chain reaction (PCR) with the consensus primers MY09/11. HPV genotypes of positive PCR products were determined by restriction fragment length polymorphisms and direct sequence analysis.

RESULTSOf the 375 swabs collected, 305 (81.3%) yielded sufficient DNA for the subsequent HPV analysis. Among the 305 subjects, the prevalence of HPV was 13.8%. Nononcogenic HPV types were found in 8.5% (26/305) of subjects, oncogenic types in 4.3% (13/305), and multiple types in 1.0% (3/305). The prevalence of HPV infection was higher in subjects from urban area than in those from rural area (P < 0.05). The prevalence was also higher in those who received fewer years of education (P < 0.05) and those who had more sex partners (P < 0.05).

CONCLUSIONSHPV infection among men at high risk is not uncommon. The detection rate of HPV DNA is significantly related to some sociodemographic factors, such as residence, educational level and the number of sex partners.

Adolescent ; Adult ; Aged ; Ambulatory Care Facilities ; China ; epidemiology ; Humans ; Male ; Middle Aged ; Papillomaviridae ; classification ; genetics ; isolation & purification ; Papillomavirus Infections ; diagnosis ; epidemiology ; virology ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; Sexually Transmitted Diseases ; prevention & control

This study was aimed to explore the change of single nucleotide polymorphism (SNP) of thrombin-activatable fibrinolysis inhibitor (TAFI) and its correlation of 2 sites (505a/g, 1040c/t) in its gene-coding region with venous thromboembolism (VTE). The genotype distribution of TAFI in 80 patients with VTE and 80 normal controls was detected by allele-specific PCR. The results showed that the distribution of each genotype of 505a/g polymorphism was not significantly different between the VTE and control groups (P > 0.05). However, t allele frequency of 1040c/t in VTE group decreased significantly as compared with the control group (40% vs 53.75%, P < 0.05), mainly due to the decrease of the proportion of tt homozygous in VTE group. It is concluded that obvious relationship is found between the polymorphism of 1040c/t in TAFI gene and VTE patients. t allele genotype may paly a protective role in VTE. The polymorphism of TAFI 505a/g may be not associated with VTE.
Adult ; Aged ; Aged, 80 and over ; Carboxypeptidase B2 ; genetics ; Case-Control Studies ; Female ; Gene Frequency ; Genotype ; Humans ; Male ; Middle Aged ; Polymorphism, Single Nucleotide ; Venous Thromboembolism ; genetics

Objective of this study was to identify gene mutation involved in a patient with type 2A von Willebrand disease (vWD). The bleeding time, vWF:Ag, FVIII:C, RIPA and multimeric assay were used for phenotypic diagnosis. All of the 52 exons and the exon-intron boundaries of vWF gene were amplified by polymerase chain reaction (PCR) and direct sequencing was carried out. The results indicated that the levels of vWF:Ag, FVIII:C and RIPA decreased in this patient, the vWF multimer with high and intermediate molecular weight was absent in plasma. The sequencing of genomic DNA revealed a C4738G (L1580V) missense mutation in the vWF gene from the patient. In conclusion, the C4738G (L1580V) missense mutation effecting the form of vWF multimer was responsible to molecular mechanism in this patient with vWD.
Adult ; Humans ; Male ; Mutation, Missense ; von Willebrand Disease, Type 2 ; genetics ; von Willebrand Factor ; analysis ; genetics

Objective:To explore the clinical characteristics of pancreaticobiliary maljunction (PBM) and its disease spectrum, and to evaluate therapeutic endoscopic retrograde cholangiopancreatography (ERCP).Methods:Data of 52 PBM patients who received therapeutic ERCP procedures for abdominal pain, jaundice and fever in Department of Gastroenterology of Peking University Third Hospital from June 2006 to March 2021 were collected. The clinical characteristics, typing, the change of disease spectrum and ERCP procedures were analyzed.Results:Among 52 PBM patients, female was more common. Abdominal pain and jaundice were the most common clinical manifestations, among which 20 were type Ⅰ, 25 type Ⅱ and 7 type Ⅲ. Half patients had the choledochal cyst. The mean timespan from the first onset to the final diagnosis was 12.2 years. Twenty-four cases (46.2%) had changes in PBM disease spectrum. Among 69 ERCP procedures, 5 (7.2%) failed. Difficult cannulation rate was 34.6% (18/52), and 11 patients underwent advanced cannulation techniques, while it was 15.4% (657/4 275) in the conterpart non-PBM patients in the same period, with significant difference between them ( χ2=14.455, P<0.05). Multiple therapeutic ERCP techniques including endoscopic sphincterotomy, pancreatic stent placement, removal of stones from the duct were applied with the successful rate of 92.8% (64/69). The incidence of post-ERCP pancreatitis was 15.4% (8/52). Conclusion:The chief clinical problem may be changed over time in PBM patients. Although ERCP plays an important role in PBM and its disease spectrum, there may be a higher rate of difficult cannulation and postoperative complications.

Objective To comparative evaluation of root canal filling paste AH-plus and zinc oxide root canal of clove oil seal performance. Methods By computer, we retrieved PubMed, CNKI and controlled clinical trial databases to find out the standard randomised controlled trial, and literature retrieval time from building library until October 20, 2014. According to the Cochrane literature quality evaluation method of system for the evaluating methodlogical quality of the study, and then we extracted the relevant data and analyzed by Rev Man 5. 1 software for Meta analysis. Results Eventually the study included in 23 articles of randomized controlled trials, and a total of 4104 teeth. The results showed the pain occurred after plus treatment of AH paste group which was lower than that in the control group [OR=0. 32, 95%CI(0. 26,0. 26), P<0. 01);AH plus paste group cure rate was higher than that of the control group after intervened 1 -2 years [ OR=2. 61, 95%CI(2. 03, 2. 03), P<0. 01];the paste resin class root filling AH plus length of root tip leakage was better than that of the control group, and nail polish and wax experiment method of measuring the penetration length of the tooth had statistical significance [MD=1. 28,95%CI( -1. 48,-1. 08), P<0. 01]; India ink staining solution to measure the penetration length of the tooth had statistical significance [MD=0. 82, 95% CI(-0. 90,-0. 74),P<0. 01]. Conclusions AH plus paste in root canal therapy can significantly alleviate pain, and the long-term curative effect is good, and better than biological intermiscibility, which is an ideal root canal filling material. Due to large incorporated in the analysis of the research and the quality difference, it is necessary for large sample, multicenter, randomized, double-blind, placebo-controlled trails to further confirm the clinical curative effect.

Objective To investigate and compare collaborative behavior between physicians and nurses in stomatological hospitals, and factors influencing nurse-physician collaboration. Methods Physicians and nurses were surveyed by Nurse-Physician Collaboration Scale ( NPSC) . Factors influencing nurse-physician collaborative level were screened out by analyzing various factors with ANOVA.Results The difference of total collaborative behavior scores between physicians and nurses was not significant ( P>0.05);among the 21 items in 3 dimensions of “information exchange among patients”,“joint participation in decision-making of treatment and nursing” and “relationship between nurses and physicians”, 7 items showed differences between the two groups ( P<0. 05 ); better nurse-physician collaborative behavior appeared between physicians with extrovert character and emotional stability, and nurses with emotional stability, 3-5 years of working and professional title as registered nurse.Conclusions The difference of nurse-physician collaborative behavior in stomatological hospitals was not significant;however, education and training of collaborative behaviors, such as nurse-physician relationship, dealing with complicated situation, etc. still need to be improved; hospital managers should build a positive collaborative environment, so as to promote team cooperation between nurses and physicians.

Objective To establish the work scope of Chinese dental nurses and provide theory evidence for improving the establishment of specialized education system and certification .Methods Semi-structured in-depth interviews were conducted to fifteen experts .The data was analyzed via qualitative research to establish the work scope of Chinese dental nurses .Results Work scope of dental nurses was established which contained four dimensions as patients management , clinical cooperation , clinic management and prevention and control of hospital infection;the scope also was divided into two stages: foundation certification and specialist development .Conclusions The work scope would play an important role in improving the establishment of specialized education system and certification .