1.Changes of biological behavioral of E. coli K1 after ppk1 gene deletion.
Liang PENG ; Jiayun PAN ; Su LUO ; Zhenghui YANG ; Mufang HUANG ; Hong CAO
Journal of Southern Medical University 2014;34(7):965-968
OBJECTIVETo study the changes in biological behaviors of meningitis E. coli K1 strain E44 after deletion of polyphosphate kinase 1 (ppk1) gene and explore the role of ppk1 in the pathogenesis of E. coli K1-induced meningitis.
METHODSThe wild-type strain E. coli K1 and ppk1 deletion mutant were exposed to heat at 56 degrees celsius; for 6 min, and their survival rates were determined. The adhesion and invasion of the bacteria to human brain microvascular endothelial cells (HBMECs) were observed using electron microscopy and quantitative tests. HBMECs were co-incubated with wild-type strain or ppk1 deletion mutant, and the cytoskeleton rearrangement was observed under laser scanning confocal microscope.
RESULTSThe survival rate of the ppk1 deletion mutant was significantly lower than that of the wild-type strain after heat exposure. The ppk1 deletion mutant also showed lowered cell adhesion and invasion abilities and weakened ability to induce cytoskeleton rearrangement in HBMECs.
CONCLUSIONSppk1 gene is important for E.coli K1 for heat resistance, cell adhesion and invasion, and for inducing cytoskeletal rearrangement in HBMECs.
Brain ; cytology ; Cells, Cultured ; Cytoskeleton ; Endothelial Cells ; cytology ; microbiology ; Escherichia coli ; genetics ; physiology ; Escherichia coli Proteins ; genetics ; Gene Deletion ; Humans ; Phosphotransferases (Phosphate Group Acceptor) ; genetics
2.Effect of occupational stress on oxidation/antioxidant capacity in nurses.
Lili CAO ; Honger TIAN ; Qingdong ZHANG ; Xinyun ZHU ; Yongguo ZHAN ; Jingguo SU ; Tian XU ; Huabin ZHU ; Ling LIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2014;32(2):100-103
OBJECTIVETo investigate the effect of occupational stress on the oxidation/antioxidant capacity in nurses.
METHODSA total of 131 nurses were included as study subjects. The occupational health information collection system (based on the Internet of things) was used for measurement of occupational stress. Levels of hydroxyl free radicals and antioxidant enzymes were determined.
RESULTSThe serum level of superoxide dismutase (SOD) was the highest in nurses under the age of 30 and the lowest in those over 45 (P < 0.05). The serum levels of glutathione peroxidase (GSH-Px) and peroxidase (POD) were the highest in nurses of working age less than 5 years, followed by those of 5-15 years, and nurses with more than 25 years' working experience showed the lowest GSH-Px and POD levels (P < 0.05). Furthermore, nurses with a university (college) degree had a higher GSH-Px level and a lower POD level compared with those with junior and senior high school degrees (P < 0.05). Job prospects and job control were positive occupational stress factors for SOD. Job hazards were negative occupational stress factors for POD. Psychological satisfaction was negative occupational stress reaction for hydroxyl free radicals. Calmness was positive occupational stress reaction for SOD, and daily stress was a negative one. The positive occupational stress reactions for GSH-Px were psychological satisfaction and job satisfaction, and daily stress was negative reaction.
CONCLUSIONNurses with higher occupational stress have stronger oxidation and weaker antioxidant capacity, which intensifies oxidant-antioxidant imbalance and leads to oxidative stress damage.
Adult ; Glutathione Peroxidase ; blood ; Humans ; Malondialdehyde ; blood ; Middle Aged ; Nurses ; psychology ; Oxidative Stress ; Reactive Oxygen Species ; blood ; Stress, Psychological ; blood ; Superoxide Dismutase ; blood ; Surveys and Questionnaires
3.Analysis of correlation between occupational stress and serum monoamine neurotransmitters in nurses.
Yongguo ZHAN ; Honger TIAN ; Qingdong ZHANG ; Xinyun ZHU ; Lili CAO ; Jingguo SU ; Tian XU ; Huabin ZHU ; Ling LIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2014;32(2):96-99
OBJECTIVETo investigate the impact of occupational stress on serum monoamine neurotransmitters in nurses.
METHODSA total of 131 nurses were included as study subjects by stratified cluster sampling. The occupational health information collection system (based on the Internet of things) was used to measure occupational stress. Serum levels of monoamine neurotransmitters were also measured.
RESULTSEpinephrine (E) was negatively correlated with superior support (P < 0.05) and colleague support (P < 0.05). Negative correlation was also found between dopamine (DA) and job prospect (P < 0.05). Level of 5-hydroxytryptamine was negatively correlated with promotion opportunities (P < 0.05). Norepinephrine (NE), E, and DA were all negatively correlated with work satisfaction (P < 0.05) and positively correlated with daily stress (P < 0.01). NE and E were negatively correlated with sufficient confidence (P < 0.05) and positively correlated with physical complaints (P < 0.01). There was a negative correlation between NE and psychological satisfaction (P < 0.05).
CONCLUSIONOccupational stress in nurses is correlated with serum monoamine neurotransmitters, and it may affect serum levels of monoamine neurotransmitters to a certain extent.
Adult ; Biogenic Monoamines ; blood ; Female ; Humans ; Job Satisfaction ; Middle Aged ; Neurotransmitter Agents ; blood ; Nurses ; psychology ; Stress, Psychological ; Surveys and Questionnaires
4.Influences of daclatasvir plus asunaprevir therapy on CD4+and CD8+T cell functions in patients with chronic hepatitis C
Yu LI ; Ying TIAN ; Li SU ; Ningjia CAO ; Zhu LI ; Qing′e LIU ; Lu WANG ; Dan ZHANG ; Hong ZHANG
Chinese Journal of Microbiology and Immunology 2018;38(5):381-389
Objective To investigate the changes in CD4+and CD8+T cell functions in patients with chronic hepatitis C in response to daclatasvir plus asunaprevir therapy. Methods A total of 21 HLA-A2-restricted patients with chronic hepatitis C virus ( HCV) genotype 1b infection were enrolled in this study. All patients were treated with daclatasvir plus asunaprevir for 24 weeks. Peripheral blood samples were collected at baseline, 4 and 24 weeks post-therapy. CD4+and CD8+T cells were sorted and purified. Cytokines secreted by CD4+T cells were measured by flow cytometry. CD8+T cells were co-cultured with HCV cell culture ( HCVcc)-infected Huh7. 5 cells in both direct and indirect contact co-culture systems. The cytolytic and non-cytolytic functions of CD8+T cells were analyzed by measuring the levels lactate dehy-drogenase and cytokines in the supernatants of cell culture. Results The virological and biochemical re-sponse rates were 71. 43% (15/21) and 77. 78% (14/18) at 4 weeks post-therapy, respectively. Both rates reached 100% at 24 weeks post-therapy. Secretion of IFN-γ, TNF-α and IL-17 by CD4+T cells was significantly enhanced at 4 and 24 weeks in response to daclatasvir plus asunaprevir therapy. In contrast, IL-10 secretion by CD4+T cells did not change notably post-therapy. However, no significant differences in cy-tokine secretion were found between patients with and without virological response at 4 weeks post-therapy. Daclatasvir plus asunaprevir therapy increased the percentage of dead cells in direct contact co-culture system of CD8+T cells and HCVcc-infected Huh7. 5 cells at 4 and 24 weeks post-therapy. However, it did not af-fect the cytotoxity of CD8+T cells in indirect contact co-culture system. Moreover, IFN-γ expression in both direct and indirect contact co-culture systems was significantly increased at 4 and 24 weeks post-therapy. There was also a notable increase in the expression of TNF-α in direct contact co-culture system, while no remarkable change in TNF-α expression was detected in indirect contact co-culture system. No significant differences in cytolytic and non-cytolytic activities of CD8+T cells were found between patients with virologi-cal and without virological response at 4 weeks post-therapy. Conclusion Daclatasvir plus asunaprevir ther-apy achieves high clinical cure rate in patients with chronic hepatitis C. Inhibition of HCV replication con-tributes to the improvement of CD4+and CD8+T cell functions.
5.Preparation and Preclinical Study of 99mTc-labeled Prostate Cancer Aptamer
Ting YANG ; Ju JIAO ; ting Yin LÜ ; Qiong ZOU ; e Su CAO ; qin Shu JIANG ; Yong ZHANG
Journal of Sun Yat-sen University(Medical Sciences) 2017;38(6):848-853
[Objective]To radiolabel the PSMA aptamer A10-3.2 with 99mTc , and explore its biological characteristics in vivo and in vitro.[Methods]Using Succinimidyl 6-hydrazinonicotinate hydrochloride (SHNH) as the bifunctional chelating agent to label aptamer A10-3.2 with 99mTc, then tested for the stability in vitro, the specific uptake by prostate cancer LNCaP cells (PSMA+) , the characteristics of SPECT/CT imaging and biodistribution in LNCaP tumor-bearing NOD/SCID mice.[Results]The labeling rate and radiochemical purity of the products (99mTc-SHNH-A10-3.2) are(71.31 ± 6.78)% and 97.03%,respectively. 99mTc-SHNH-A10-3.2 had obvious target specificity for PSMA positive prostate cancer LNCaP cells, its uptake rate was significantly higher than PSMA nega?tive PC-3 cells (P<0.01). And in tumor-bearing mice, the tumor has a certain uptake and a high ratio of the tumor tissue to the mus?cle.[Conclusion]This study successfully constructed 99mTc-labeled PSMA-targeted aptamer A10-3.2, which has a good stability and targeting in vivo and in vitro, has a high tumor tissue/muscle ratio in tumor-bearing mice, which show that it may be a potential target?ed molecular imaging agent for prostate cancer.
6. The rapeutic effect of Cefpodoxime proxetil granules in treatment of acute bronchitis in children
Haixia SU ; Yanfeng SU ; Feng′e CAO
Chinese Journal of Applied Clinical Pediatrics 2019;34(15):1182-1185
Objective:
To study the efficacy and safety of Cefpodoxime proxetil granules in the treatment of acute bronchitis in children.
Methods:
One hundred and sixty children of the First People′s Hospital of Nanning from June to December 2018 with acute bronchitis were randomly divided into cefpodoxime group and cefaclor group, with 80 cases in each group.All patients received routine treatment.On this basis, 80 patients in the cefpodoxime group received oral Cefpodoxime proxetil granules 5 mg/kg (not more than 100 mg/time), twice a day; 80 patients in the cefaclor group received oral Cefaclor.The granules were 10 mg/kg (not more than 250 mg/time), 3 times a day, and the course of treatment was 5 days.The clinical efficacy and adverse reactions were observed.
Results:
The cure rate and effective rate of Cefpodoxime group were 91.3% (73/80 cases) and 95.0% (76/80 cases), respectively, while the cure rate and effective rate of Cefaclor group were 66.3%(53/80 cases) and 81.3%(65/80 cases), respectively, and the differences between the 2 groups were statistically significant (
7.Effects of Two Placement Ways for Storage of Blood Bag on Biochemical Indexes of Leukodepleted Red Blood Cells.
Rui-Jun ZHANG ; Bing-Zheng DUAN ; Chun-Mei JU ; Su-Qin SUI ; Yan BAI ; Huan CAO
Journal of Experimental Hematology 2016;24(2):607-610
OBJECTIVETo investigate the effects of 2 different ways of storage bag placement on some biochemical indexes of leukodepleted red blood cells (LD-RBC) to as to ensure the efficacy and safety of clinical blood transfusion.
METHODSThe whole blood samples of 20 donors (400 ml/donor) were selected for preparating the LP-RBC, which were divided evenly into 10 bags. The 10 bags were randomly divided into 2 groups; the bags in 1 group were placed uprightly, while the bags in another group were placed horizontally. The bags of 2 groups were stored in the same conditions. One storage bag from each group was taken randomly on day 7, 14, 21, 28, 35 respectively, and then the biochemical indexes of samples were detected and analyzed.
RESULTSThe values of K(+) and LAC on day 14, the value of LDH on day 28 in the uprightly placed group were higher than those in the horizontally placed group (P < 0.05), the value of Na(+) on day 28, and the value of Glu on day 35 in the uprightly placed group were lower than those in horizontally placed group (P < 0.05), but there was no significant difference in Cl(-) level between 2 groups (P > 0.05).
CONCLUSIONThe storage bags placed by different ways during the storage show different influence on some biochemical indexes of LD-RBC in the storage period.
Blood Specimen Collection ; instrumentation ; methods ; Blood Transfusion ; Erythrocytes ; Humans ; Random Allocation
8.Association of XRCC1 genetic polymorphism with susceptibility to non-Hodgkin's lymphoma.
Su-Xia LI ; Hong-Li ZHU ; Bo GUO ; Yang YANG ; Hong-Yan WANG ; Jing-Fen SUN ; Yong-Bin CAO
Journal of Experimental Hematology 2014;22(4):982-987
The purpose of this study was to explore the association between X-ray repair cross-complementing group 1 (XRCC1)gene polymorphism and non-Hodgkin's lymphoma risk. A total of 282 non-Hodgkin's lymphoma (NHL) patients and 231 normal controls were used to investigate the effect of three XRCC1 gene polymorphisms (rs25487, rs25489, rs1799782) on susceptibility to non-Hodgkin's lymphoma. Genotyping was performed by using SNaPshot method. All statistical analyses were done with R software. Genotype and allele frequencies of XRCC1 were compared between the patients and controls by using the chi-square test. Crude and adjusted odd ratios and 95% confidence intervals were calculated by using logistic regression on the basis of genetic different models. For four kinds of NHL, subgroup analyses were also conducted. Combined genotype analyses of the three XRCC1 polymorphisms were also done by using logistic regression. The results showed that the variant genotype frequency was not significantly different between the controls and NHL or NHL subtype cases. Combined genotype analyses of XRCC1 399-280-194 results showed that the combined genotype was not associated with risk of NHL overall, but the VT-WT-WT combined genotype was associated with the decreased risk of T-NHL (OR: 0.21; 95%CI (0.06-0.8); P = 0.022), and the WT-VT-WT combined genotype was associated with the increased risk of FL(OR:15.23; 95%CI (1.69-137.39); P = 0.015). It is concluded that any studied polymorphism (rs25487, rs25489, rs1799782) alone was not shown to be rela-ted with the risk of NHL or each histologic subtype of NHL. The combined genotype with mutation of three SNP of XRCC1 was not related to the risk of NHL. However, further large-scale studies would be needed to confirm the association of decreased or increased risk for T-NHL and FL with the risk 3 combined SNP mutants of XRCC1 polymorphism.
Case-Control Studies
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China
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epidemiology
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DNA Repair
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DNA-Binding Proteins
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genetics
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Female
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Humans
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Lymphoma, Non-Hodgkin
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epidemiology
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genetics
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Male
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Middle Aged
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Polymorphism, Single Nucleotide
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Risk Factors
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X-ray Repair Cross Complementing Protein 1
9.Establishment of mouse mesenchymal stem cells overexpressing CXCR4 gene and evaluation of their functions.
Wei CHEN ; Miao LI ; Gui-Zhen SU ; Jiang CAO ; Wei SANG ; Kai ZHAO ; Qing-Yun WU ; Feng ZHU ; Kai-Lin XU
Journal of Experimental Hematology 2014;22(5):1391-1395
This study was purposed to establish the mesenchymal stem cells (MSCs) stably overexpressing mouse CXC chemokine receptor type 4 (CXCR4) gene and to explore their function. The recombinant lentiviral vector LV-CXCR4-IRES-EGFP with packaging plasmid pSPAX2 and envelope plasmid pMD.2G were co-transfected into 293FT packaging cell line using lipofectamine 2000 to produce the recombinant lentiviral vectors. The recombinant viruses were harvested and concentrated by using ultracentrifugation. Mouse bone marrow MSC were infected with the viral supernatants. Variable methods were used to optimize the transduction condition. EGFP expression was visualized using fluorescence microscope and efficiency of infection was determined by flow cytometry (FCM). Proliferation and apoptosis were detected by proliferation curve and FCM, respectively. Migration capacity was assessed by a chemotaxis assay using transwell. Expression of EGFP were detected by fluorescence microscopy in MSCs after infection. The results showed that through optimization of infection condition, the recombination lentiviral vectors had higher infection efficacy; after infection for 72 h, the higher expression of EGFP could be observed under fluorescence microscope; the expression of CXCR4 protein on MSC surface in CXCR4-MSC group significantly increased compared with those in the control group. Meanwhile, over-expression of CXCR4 had no effect on their capacity of proliferation and did not induce apoptosis. Moreover, CXCR4 enhanced the migration of cells in the transwell induced by SDF-1 gradient compared with the EGFP control group. It is concluded that the lentiviral vector can not only infect mouse MSCs efficiently, but also can make CXCR4 express stably in MSC.
Animals
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Apoptosis
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Cell Line
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Chemokine CXCL12
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Flow Cytometry
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Genetic Vectors
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Lentivirus
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Mesenchymal Stromal Cells
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metabolism
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Mice
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Plasmids
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Receptors, CXCR4
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genetics
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Transfection