Objective To construct a recombinant plasmid pLEGFP-mIFN? for developing the genetical therapy of tumor by stem cells.Methods PCR was used to amplify the mIFN? gene from plasmid pcDNA3.1-mIFN? in which the mIFN? was cloned.A new plasmid,pLEGFP-mIFN?,was then constructed by inserting the amplified mIFN? gene into pLEGFP-C1.Restriction analysis and sequencing were used to confirm the structure of pLEGFP-mIFN?.Neural stem cells were transfected by supernatant of PA317.Results A DNA fragment in size of 500 bp was amplified.Restriction analysis showed that the amplified gene was inserted into pLEGFP-C1 correctly.(Conclusion The) plasmid pLEGFP-mIFN? is constructed successfully,and it can transfect neural stem cells.

Objective To investigate the mechanism of liver X receptor(LXR)signal pathway in regulating glucose metabolism by observing the variety of LXR expression and its impacts on regulating the mRNA expression of PEPCK and GCK, the key enzyme of hepatic glucose metabolism in various glucose metabolic status in rats. Methods SD rats were chosen and divided into four groups:the CON group, the induced DM group, the OB group, and the induced OB+DM group. For each group of rats, body weight, blood glucose, serum triglycerides, and cholesterol were measured. Then the rats were sacrificed and the livers were collected and studied. Real-time PGR was used to measure the expressions of LXR mRNA, PEPCK mRNA, and GCK mRNA in the livers. Finally, the Western Blot assay was used to measure the liver LXR protein expression. Results The expression of LXR mRNA was significantly higher in DM,OB, and OB+DM groups than in CON group(P＜0.05).The Western blot results showed that the levels of protein were in accordance with the mRNA expression. Comparing to the CON and the OB groups, the PEPCK mRNA expression of the OB+DM and the DM groups was significantly higher, while the GCK mRNA expression of these two groups was significantly lower(P＜0.05). Comparing to the CON group, the PEPCK mRNA expression of the OB group was significantly lower, while the GCK mRNA expression of OB group was significantly higher(P＜0.05).Conclusions During non-diabetic phase, LXR could act as a protective receptor for glucose metabolism and keep glucose homeostasis by regulating the key enzymes of the hepatic glucose metabolism. While in the diabetic phase, the protective receptor LXR failed to reverse the change of the related enzymes caused by insulin deficiency, and finally the plasma glucose level was raised.

Twenty-seven male SD rats were assigned to 3 groups: control, obese, and obese with diabetes groups. Serum corticosterone was determined and euglycemic-hyperinsulinemic clamp was carried out. Expressions of liver X receptor ( LXR), 11 β-hydroxysteroid dehydrogenase 1 ( 11 β-HSD1 ), and glucocorticoid receptor (GR) were determined by real-time PCR. Both groups of obese rats were insulin-resistant, with up-regulated LXR mRNA and down-regulated 11 β-HSD1 mRNA( both P＜0.05 ).

Objective To investigate the clinical characteristics,treatment and prognosis of myocardial damage presented with abdominal pain in children.Methods The clinical data of 33 children with myocardial damage presented with abdominal pain in Beijing Anzhen Hospital during the past 4 years were analyzed.Emergency conditions were ruled out in all cases.Results Among 33 cases with myocardial damage presented with abdominal pain,there were 10 cases of pneumonia,9 cases of acute bronchitis,7 cases of upper respiratory tract infection,1 case of allergic purpura,1 case of myocarditis and 5 cases of unknown causes.The duration of abdominal pain lasted 2 days to 3 months.The characteristics and the duration of abdominal pain were irregular.Mycoplasma antibodies positive were 5 cases (2 cases of bronchitis,3 cases of pneumonia).Coxvirus antibodies were 2 cases.There were different symptoms and signs of myocardial damage.Holter:there were ST-T changes in 23 children and 17 frequent premature ventricular complex(pattern of bigeminy and trigeminy in 7 children,premature atrial complex in 4 children).Creatine kinase isoenzyme-MB were high in 21 children(30-124 U?L-1).Cardiac troponin I were high in 19 children(0.8-1.2 ng?L-1).Chest X-ray:cardiomegaly was noted in 7 children,and the ratio of cardiac and chest were 0.55-0.58.Echocardiography:the left ventricular end-diastolic diameter was enlarged in 5 children,the right atrium was enlarged in 1 child.Most abdominal pain was relieved with treatment in 5 to 7 days,and 14 days was the longest.Total treatment course was 2 weeks to 1 month,and 3 months was the longest.But 1 case with myocarditis was recurrent abdominal pain treated nearly 3 months,and lasted to 6 months for recovery.Conclusions It is important to pay attention to cardial examination early,when a child becomes inconsolable from abdominal pain but emergency conditions should be ruled out.

Objective To investigate the accuracy of computer-assisted interpretation(CAI)in the diagnosis of pediatric critical arrhythmia,and to establish Logistic regression model to evaluate the main risk factors of CAI error.Methods 1147 cases of arrhythmia patients were divided into heart disease(HD)and nonheart disease group(NHD).Candidate risk factors were chosen,from which the vailables statistically significant were entered for logistic regression analysis.Results CAI error rate of HD group and NHD group were 24.9%and 11.4%,showing a significant difference(P＜0.05).Age,underlying diseases,the number of CAI diagnosis entries were the risk factors.Conclusion CAI error pattern was different among HD group and the NHD group.Higher awareness for CAI error risk factors and CAI technology improvement are critical for a lower CAI error rate.

Objective:To investigate the application value of thromboelastography (TEG) in choosing antiplatelet drug for patients with cerebral arterial thrombosis.Methods: 100 new patients with cerebral arterial thrombosis, who received single antiplatelet therapy after received dual antiplatelet therapy (aspirin and clopidogrel) in acute stage (14d), were randomly divided into observation group (50cases)and control group (50cases). According to different method of choosing drug, patients of control group randomly were implemented different single antiplatelet drug, while patients of observation group received more sensitive antiplatelet drug based on TEG results. The follow-up observation about recurrent situation of cerebral arterial thrombosis for the two groups were carried out in further study.Results: In one year, the recurrence rate of cerebral arterial thrombosis of observation group (6%, 3/50) was significantly lower than that of control group (20%, 10/50) (x2=4.332,P<0.05). The differences of recurrence rate between different antiplatelet drug in intra-group were not statistically significant.Conclusion: TEG can efficiently evaluate the therapeutic effect of antiplatelet drug, and guide the selection for drug so as to reduce the recurrence rate of cerebral arterial thrombosis.

OBJECTIVE To determine the activity of moxifloxacin on bacterial biofilms formed by Haemophilus influenzae in vitro.METHODS Formation of bacterial biofilm was examined by crystal violet assay,viable cells counting in biofilms was also carried out.Alterations of biofilms were measured under varying concentrations of moxifloxacin.RESULTS Optical density values of biofilms were significantly decreased at the concentrations higher than 4 mg/L of moxifloxacin.The similar results were obtained for viable cell counting.Bacteria in biofilms were eliminated partly or completely at concentrations higher than 0.25 mg/L.CONCLUSIONS Moxifloxacin is able to destroy the biofilms and reduce viable cells counting at high concentrations.

Acute Disease ; Adolescent ; Adult ; Child ; Child, Preschool ; Female ; Humans ; Male ; Middle Aged ; Mushroom Poisoning ; diagnostic imaging ; Ultrasonography

Objective To investigate the effect of persistent concept based on strong concept on long-term hope of patients with breast cancer undergoing extended-concept nursing.Methods Two hundred and sixty-six patients with breast cancer were randomly divided into control group (n=62) and observation group (n=64) according to the discharge order.The routine health education and instruction were carried out plus strong concept of nursing intervention for three times during hospitalization.About 2 months after discharge the observation group was treated with the strong concept of nursing intervention (1 month,4 times) and the control group without strong concept of nursing intervention.The two groups were assessed with Hirth hope index (HHI) at discharge and six months after discharge.Results There was no significant difference in hope level between the two groups (P ＞ 0.05)at discharge.Six months after discharge,the scores of hope in the observation group were significantly higher than those in the control group (P ＜ 0.05) and with out statistical sigficance when comaparing to who at discharge(P ＞ 0.05).The HHI score of the control group 6 moths after discharge was significantly lower than that of the control group at discharge (P ＜ 0.05).Conclusion The strong concept nursing intervention during hospitalization can effectively improve the hope level of patients with breast cancer surgery.

Objective To discuss the application of Occupational Functioning Model (OFM) in occupational therapy teaching. Methods The students in grade 2009 and 2010 for their course of Occupational Therapy Clinical Application were included. The former were taught in routine way, and the latter were taught with OFM. They were investigated with questionnaires of satisfaction of course and teaching. Results There were 94.1% of the students felt beneficial in the clinical thinking, 86.3% in the comprehensive quality. There was more in the grade 2010 than 2009 of students reported to be benefited in such as guidance of the occupation mode for the clinical practice, independent thinking, clinical thinking, and the capability of evaluation and plan of systemic rehabilitation (P<0.05). Conclusion OFM may help students to understand occupational therapy deeply, develop the clinical thinking, and improve the comprehensive quality.