Humans ; Nerve Growth Factor ; physiology ; Pain ; physiopathology ; Pulpitis ; physiopathology ; Receptor, Nerve Growth Factor ; physiology ; Receptor, trkA ; physiology

Objective A series of 5 patients with central pontine myelinolysis (CPM) confirmed either by cranial MRI,CT image (4 cases) or by autopsy pathological analysis (1 case) was reported. The pathophysiological mechanism,the early diagnosis and the role in prevention were discussed. Methods The creteria obtained from clinical features, cranial MRI and CT imagine as well as autopsy pathological changes were thoroughly analyzed. Results ① All 5 case of CPM were occurring in patients who had already had somatic disease and serious systemic functional failure, especially in those who had had seriously water and ion disturbances, such as hyponatremia. ② In 4 of the 5 patients, psychiatric manifestation tended to show the earliest clinical features. ③ The pseudobulbaral paralysis appeared in 4 patients. ④ Cranial MRI and CT served as the most reliable techniques for diagnosing CPM cases when patients are still alive. ⑤ A variety of paralysis were found in all of the 5 CPM patients. ⑥Pathological analysis demonstrated that there is a symmetric unspecific myelinolysis in areas of basal pontine, caudal middle brain and the superior medulla (1 patient). Conclusion ① The occurrence of hyponatrimia and its too fast correction may be associated with the onset of CPM. ② CPM, a self-restrained disease can be cured from dangerous complications, such as pneumonia, respiratory failure and malnutrition when suitably treated.

Target of the treatment for advanced pancreatic carcinoma is always palliative without prolongation of survival time almost, therefore the aims of treatment should be concentrated on improving the quality of life and alleviateing the disease-related symptoms. We do not call for the treatment and medicines that can incur obvious adverse reaction with limited effectiveness. Interventional therapy provides superexcellent short term efficacy with alleviating the disease-related symptoms significantly, and without severe side effects and thus coincides to the principle of the treatment for advanced pancreatic carcinoma. The surpassing advantages provide popular recommendation for this very kind of therapy in the recent years.

Objective To evaluate the therapeutic efficacy of microendoscopic tubular retractor system (METRx) with a postmedial approach in the treatment of lumbar disc herniation. Methods A total of 290 patients with lumbar disc herniation were treated with METRx. The duration of follow-up ranged from 3 months to 33 months. Results According to the Nakai scale, 81% (235 cases) of the patients were evaluated as excellent, 13.4% (39 cases) as good, 4.8% (14cases) as fair and 0.7% (2 cases) as poor. Postoperative infection in intervertebral space was found in 2 cases. Conclusions METRx has a satisfactory short-term therapeutic efficacy in the treatment of lumbar disc herniation.

Objective To investigate the correlation between level of Titin antibodies (Titin-Ab) and patient's condition and different thymic pathological patterns in patients with myasthenia gravis (MG), and to assess its value in the diagnosis and prognosis of MG.Methods The levels of Titin-Ab in serum from 52 patients with MG (MG group), 18 cases with other neurologic diseases (NMG group) and 50 normal controls (NC group) were detected using enzyme-linked immunosorbentassay (ELISA). 10 cases with MG who received operation on thymus were tested again after therapy.Results The positive rate of Titin-Ab in MG group was 36.5%,but not present in MNG and NC groups.The MG group!was significantly higher than those in NMG group and NC group (all P

Objective To explore the typing,precaution and treatment of the bone cement leakage caused by percutaneous vertebroplasty(PVP).Methods PVP was performed in 38 cases(11 men and 27 women) with vertebral angioma and vertebral body compression fracture with osteoporosis chosen from November 2002.Results All the patients were followed up for 8 to 16 months and the effects were evaluated by the standards carried out by World Health Organization(WHO).The rate of complete response(CR) plus partial response(PR) was 100 percent.The patients with bone cement leakage by PVP were divided into 3 groups which underwent different remedial methods respectively.Conclusion The bone cement leakage could be prevented through the preoperative preparation,choosing the anaesthesia and scouting the whole operation.The patients with TypeⅠ,Ⅱ and Ⅲ_a needn't treatment,but those with type Ⅲ_b must accept open-operation.Vertebral eggshell technology with open vertebroplasty was effective on the treatment of the bone cement leakage with type Ⅲ_b caused by PVP.

Acupuncture Points ; Adult ; Electroacupuncture ; Female ; Head ; innervation ; Humans ; Neuritis ; therapy

Objective To investigate the apoptotic effect induced by sulfur mustard on the lymphocytes of the rat spleen,and define the role of caspase-3 during the process.Methods Sulfur mustard was given by intraperitoneal injection in a dose of 3.5mg/kg.The animals were anesthetized and the spleens were harvested at different timepoints after intoxication.The histopathogy of spleen was studied with hematoxylin-eosin staining.Caspase-3 mRNA was detected with RT-PCR.Protein expression of caspase-3 was assayed with Western blotting,lymphocytes of rat spleen were isolated and cultured in vitro and they were challenged with sulfur mustard(100?mol/L).The effect of Ac-DEVD-CHO(a specific inhibitor of caspase-3)on sulfur mustard-induced apoptosis of the lymphocytes cultured in vitro was evaluated.Fluorescent probe labeled with Rhodamine 123 was used to study mitochondrial potential.Results The histology of rat spleen was affected after sulfur mustard intoxication,as evidenced by apoptosis of a part of lymphocytes.Protein and mRNA expressions of caspase-3 were increased significantly in the spleens of intoxicated rats as compared with that in control group.DNA ladder and 'sub-G1' peak of lymphocytes which were treated with sulfur mustard in vitro were partially improved by Ac-DEVD-CHO(the specific inhibitor of caspase-3).In addition,mitochondrial potential decreased in a time-dependent manner in the lymphocytes intoxicated by sulfur mustard in vitro compared with that in the control group.Conclusions The spleen is injured in the rat which is intoxicated by sulfur mustard.Lymphocyte apoptosis is one of the mechanisms splenic injury.Caspase-3 may be involved in the process of lymphocyte apoptosis induced by sulfur mustard.

Polymerase chain reaction was employed to amplify the HBV X gene from plasmid pCP10, and the product was cloned into pVR1012, then transfected HepG2 cells and cotransfected HepG2 cells with reporter plasmid pSV lacZ HBx protein produced by HepG2 cells was measured by ELISA method The activity of ? galactosidase was measured by a kit, which reflected the transactivating function of HBx protein The results showed that HepG2 cells transfected by pVR1012 X could express HBx protein The expression of ? galactosidase in HepG2 cells transfected by the pVR1012 X was 3 2 fold higher as that of control plasmid It is suggested that the recombinant plasmid pVR1012 X can be expressed in mammalian cell line, and has transactivating effect on SV40 early promoter

Objective: To study the microstructure of e-polytetra fluoroethylene (e-PTFE) membrane and chitin membrane and to observe the growth of osteoblasts on the membrane. Methods: Light microscope and scanning electronic microscope were used to study the microstructure of e-PTFE membrane and chitin membrane. Cell culture methods and cell counting were used to observe the growth of osteoblasts on the two kinds of membrane. Results: The chitin membrane showed a compact structure without any pore in it. There were many shallow dents with the diameter of 5-8 ?m on it's surface. However, in e-PTFE membrane many tiny lined cracks (1-5 ?m in width and 5-15 ?m in length) in elliptic structure were observed. The adhesion and proliferation of osteoblasts on the two kinds of membranes were both well. ALP measurement showed that there were no significant difference between each of the two membrane groups and the control. Conclusions: Both membranes have no negative effects on the growth of osteoblasts. Both membranes are biocompatibile and their microstructure is appropriate for guided bone regeneration materials.