According to the project of personnel cultivation in 21st century,we have increased interdisciplinary comprehensive experimental courses to intensify the practical education,optimize operating model and teaching method of experimental courses.Experimental education must be strengthened in order to culture students' creativity.

Industrial culture media for cultivation of marine microalgae Nannochloropsis oculata was optimized by using Random Design software. High eicosapentaenoic acid (EPA) content was obtained over 40% of total fatty acid, and high average specific growth rate of 0.32d -1 was obtained in cells grown in the selected Formula 1. Mass cultivation of this species was practiced in indoor open raceway ponds of 6.12 m 2 and 28.02 m 2, and in outdoor open pond of 83.50 m 2 during May and June in 1999. The average area yield of biomass were obtained at 2.77, 1.62 and 2.52 g /m 2?d, and in October, 1.09 g/ m 2?d was obtained in 83.5m 2 outdoor pond, and the harvest algae powder contained 49.50% of protein, 20.30% of total lipid and 3.00% of EPA of dry weight. With high temperature tolerant species during August in 2000, the average area yield of biomass were increased to 5.44?9.96 and 7.66 g/ m 2?d in above ponds, respectively and 2.50% of EPA of dry weight was contained in the powder. It is indicated that the industrialized cultivation of Nannochloropsis oculata rich in EPA is completely doable on practice, and the algal powder was a good raw material as single cell protein and development of EPA rich products.

Adult ; Cerebral Ventricle Neoplasms ; diagnosis ; metabolism ; Diagnosis, Differential ; Glial Fibrillary Acidic Protein ; analysis ; Humans ; Immunohistochemistry ; Lateral Ventricles ; chemistry ; pathology ; Male

Anion Exchange Protein 1, Erythrocyte ; metabolism ; Antiporters ; metabolism ; Child ; Chordoma ; metabolism ; pathology ; radiotherapy ; surgery ; Female ; Follow-Up Studies ; Humans ; Skull Base Neoplasms ; metabolism ; pathology ; radiotherapy ; surgery ; Vimentin ; metabolism

Chronic Disease ; Forkhead Transcription Factors ; Hepatitis, Viral, Human ; Humans

Adult ; Carbodiimides ; poisoning ; Humans ; Male ; Methylamines ; poisoning ; Occupational Exposure ; Young Adult

Animals ; Dyslipidemias ; embryology ; physiopathology ; Humans ; Renal Insufficiency, Chronic ; epidemiology ; physiopathology ; therapy ; Renal Replacement Therapy

Animals ; Epithelial Cells ; cytology ; drug effects ; Humans ; Kidney Tubules ; cytology ; drug effects ; Oleanolic Acid ; analogs & derivatives ; pharmacology ; Podocytes ; drug effects

A monoclonal antibody (McAb) 3H11 against gastric cancer with high positive rate, high selectivity and high affinity was obtained. 131 I Labeled McAb 3H11 was injected into gastric cancer-bearing nude mice, and the tumor/liver ratio, localization index, ID%/g could reach 8.26?1.26, 6.08 + 1.51, 11.00?2.62, respectively. Moreover, McAb 3H11 conjugated with 131 I could increase the cytotoxicity to tumor xenografts and decrease the side effects. The radioimmunoimage was carried out in 19 cases with gastric cancer, and positive image was obtained in 16 cases (84.2%). The radioimmunoguided surgery of gastric cancer was successfully done by submucous injection of labeled McAb 3H11 through endoscope. The sensitivity, specificity and accuracy for evaluation of tumor infiltrated gastric walls were 94.6%, 96.7% and 95.9%, respectively. The sensitivity, specificity and accuracy for identification of metastatic lymph nodes were 99.2% ,97.7% and 98.8%, respectively.

Objective To evaluate the value of serologic teats in patients with primary biliary cirrhosis by conducting a seroprevalence survey for anti-gp210 and anti-sp100 antibodies.Methods A total of 72 consecutive primary biliary cirrhosis (PBC) patients with or without Sj(o)gren's syndrome (SS) and 50 patho logic controls were studied.Antibodies were tested by ELISA assays with recombinant spl00 and purified gp210.Results The positive rates of anti-gp210 detected by ELISA was 31.1% and 45.5% in PBC patients with and without SS respectively.Among SS and virus hepatitis patients,none had anti-gp210 antibody (P＜0.01).The prevalence of anti-gp210 was similar in PBC patients with and without SS.The positive rates of an ti-sp100 detected by ELISA was 14.8% and 18.2% in PBC patients with and without SS respectively.Among the patients with SS,only 3.3% was positive for anti-sp100,but none had anti-sp100 reactivity in virus hep atitis patients.The prevalence of anti-sp100 was not significantly different between PBC and SS groups. Conclusion Anti-gp210 and anti-sp100 are highly specific for PBC.The sensitivity of anti-gp210 and anti sp100 is 31.1% and 14.8% respectively.They may be helpful in the diagnosis of PBC.