2.Observation of aerobic exercise effects improved on serum lipids in diet-induced hyperlipidemia mice.
Guo-Dong MA ; Yan-Huan LIU ; Zhong DING
Chinese Journal of Applied Physiology 2004;20(1):41-74
Animals
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Diet
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adverse effects
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Hyperlipidemias
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blood
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etiology
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Lipids
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blood
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Male
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Mice
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Mice, Inbred C57BL
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Physical Conditioning, Animal
3.Effect of flunarizine on the epileptiform discharges in cortex and hippocampus of rat with penicillin induced epilepsy.
Guo-feng WU ; Shi-hai SHAN ; You-zhong DONG
Chinese Journal of Applied Physiology 2004;20(2):179-204
Animals
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Cerebral Cortex
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drug effects
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physiology
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Epilepsy
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chemically induced
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physiopathology
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Female
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Flunarizine
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pharmacology
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Hippocampus
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drug effects
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physiology
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Male
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Penicillins
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adverse effects
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Rats
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Rats, Wistar
5.Research progress on tumor-vessel associated extravascular tumor metastasis
Wenwen GUO ; Haiming WEI ; Xianglan MO ; Xiaogang ZHONG ; Xiaofeng DONG
Chinese Journal of Digestive Surgery 2017;16(3):313-315
The occurrence of malignant tunors is increasing annually around the world.In every year,8.2 million patients died of malignant tumors in which about 90% patients died of malignant tumors associated multiple organs metastases.Inhibiting tumor metastasis is the key event to prolong the survival time of patients.With the further research,tumor-vessel associated extravascular tumor metastasis is playing an increasingly important role in the clinical application.This paper summarizes the new developments of tumor-vessel associated extravascular tumor metastasis to provide possible ideas for the therapy of malignant tumors.
6.Efficacy of endovascular interventional minimally invasive techniques in treatment of acute brachial artery injury
Xiquan ZHANG ; Shitang GE ; Zhong CHEN ; Ge DONG ; Feng GUO
Chinese Journal of Trauma 2017;33(3):281-284
Objective To evaluate the efficacy of endovascular interventional minimally invasive techniques in treatment of acute brachial artery injury.Methods A retrospective case series study was designed to analyzed data of 16 cases (11 males and 5 females) sustaining acute brachial artery injury treated from March 2011 to August 2016.Mean age was 47.9 years (range,27-66 years).Left upper extremity vascular injury occurred in 7 cases and right upper extremity vascular injury occurred in 9.There were 13 cases of arterial intima injury and 3 of arterial pseudoaneurysm.All patients underwent endovascular therapy including stent implantation and balloon dilatation.Angiography was used to evaluate the repair of brachial artery and the operation time was recorded.Improvements of distal limb arterial pulse and limb ischemic symptoms were observed,and the patients who were followed up with stent lumen stenosis more than 50% received endovascular treatment again.Results The procedures was successful in all patients,with operation time of 30-75 minutes (mean,45 minutes).After the insertion of a stent,recovered brachial artery blood flow and disappeared pseudoaneurysm were observed on angiography,without occurrence of stent stenosis or contrast extravasation.Symptoms of local pain and numbness were alleviated,and blood supply of the distal brachial artery was improved after operation.All patients were followed up for 3-36 months(mean,16.7 months).The false aneurysm did not recur.Two cases of stent stenosis more than 50% had a second balloon dilation procedure and the blood flow returned to normal.Stent patency was found in 14 cases with stent stenosis of less than 50%.During the follow-up period,no ischemic necrosis,displacement of the stent or stent fracture were found.Conclusion For acute brachial artery injury,endovascular management exhibits higher technical success rate and good clinical outcome with a low incidence of complications.
7.Screening of marker gene in Yersinia pestis plasmid
Shan-shan, DONG ; Ying, GUO ; Peng, WANG ; Zhi-zhong, SONG
Chinese Journal of Endemiology 2012;31(5):490-494
Objective To screen the conservative,stable and specific DNA signature sequence in the plasmid of Yersinia pestis.Methods Specific validation trials and stability of the qualification test were carried out to 40 strains of Yersinia pestis,47 strains of non-Yersinia pestis of home and wild types of rodent in Yunnan,by using 32 DNA sequences derived from Yersinia pestis in the plasmid and conventional PCR technology,and Yersinia pestis vaccine strain EV76 as a positive control.Results Four pairs of relatively conservative,stable and specific DNA marker genes were screened:YPMT1.05c,YPMT1.03c,YPMT1.42 and YPMT1.04c.Conclusions The 4 pairs of Yersinia pestis DNA signature sequences can be used for rapid diagnosis of plague.
8.Isolation and Characterization of a Moderately Halophilic Bacterium High-producing Esterase
Wei DONG ; Li-Zhong GUO ; Cui-Cui LI ; Lin WANG ; Wei-Dong LU ;
Microbiology 1992;0(04):-
A moderately halophilic bacterium strain DF-B6 capable of degrading Tween 20 was isolated from solar saltern brine by plate method.Based on the phenotype,physiological and biochemical character-istics,and the phylogenetic analysis of 16S rDNA sequence,strain DF-B6 was identified as Idiomarina.The substrate specificity test showed that the lipolytic enzyme from strain DF-B6 was an esterase,and not a li-pase.The maximum esterase activity was observed in 8% NaCl concentration at 50?C,pH 8.0.The effect of various divalent cations was studied on the activity of esterase from Idiomarina sp.DF-B6 at the concentra-tion of 10 mmol/L,Mg2+,Ca2+ and Mn2+enhanced the esterase activity,while Zn2+,Fe3+ and Cu2+ inhibited its activity.
9.Design, synthesis and pharmacological investigation of isoindoline derivatives as 5-HT/NE double reuptake inhibitors.
Hui WEN ; Yuan SHI ; Jing-wen DONG ; Yan-shen GUO ; Jian-Jun ZANG ; Guang-zhong YANG
Acta Pharmaceutica Sinica 2015;50(9):1148-1155
A series of isoindoline derivatives were designed, synthesized, and evaluated for their double inhibitory activities. All of them were new compounds, and their structures were confirmed by 1H NMR and HR-MS. Preliminary in vitro pharmacological tests showed that all compounds exhibited 5-HT or NE reuptake inhibition activity. Among the tested compounds, compound I-3 exhibited potent inhibitory activity against 5-HT and NE reuptake in vitro, and exhibited potent antidepressant activity in vivo. These compounds designed can be further optimized for finding more potent 5-HT/NE dual reuptake inhibitors and antidepressant candidates as well.
Antidepressive Agents
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chemical synthesis
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chemistry
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Biological Transport
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Drug Design
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Isoindoles
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chemical synthesis
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chemistry
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Serotonin Uptake Inhibitors
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chemical synthesis
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chemistry
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Structure-Activity Relationship