A major epidemic of DF/DHF occurred at Ha Noi in 1998, and 3348 cases of have been reported. Serological surveillance confirmed that the epidemic of dengue fever was caused by dengue 3 and dengue 1 virus types 32/37 positive samples were dengue 3 (86.48%). Dengue virus type 3 was isolated in 6 districts of Ha Noi
Dengue Virus ; Dengue ; epidemiology

Objective: Dengue associated hypokalemic paralysis (DHP) is an unusual neurological complication of dengue fever. This was a retrospective study of patients with DHP compared with idiopathic hypokalemic paralysis (IHP) seen in a tertiary centre in North India. Methods: Dengue was diagnosed by positive nonstructural protein (NS) 1 antigen and dengue-IgM antibody. Various clinical and laboratory parameters were compared between patients with DHP and IHP. Results: DHP was seen in 18 out of 489 (3.7%) dengue patients seen in the 4-years study period. Complications of bulbar weakness or respiratory failure developed in 6/18 (33.3%) of DHP patients. DHP patients with complications had higher hematocrit (p<0.001), lower platelet count (p=0.002), lower serum potassium (p=0.007) and higher creatinekinase (CK) (p<0.001) as compared to those without complications. When compared to IHP, DHP patients had fever at admission (p<0.001); myalgia (p<0.001); no past episodes (p=0.032); greater disability (p=0.02); lower serum potassium (p=0.0338); higher CK (p=0.001); and nerve conduction abnormalities (p=0.035). Conclusions: DHP should be suspected in patients who present with acute onset muscle weakness associated with fever. Predictors of complications in DHP include high hematocrit, low platelet count, low serum potassium and raised CK.
Dengue Virus ; Dengue

BACKGROUND: The DOH has recently launched the first ever dengue vaccine that has successfully completed phase III clinical trials but an assessment of the general acceptance of the vaccine is widely lacking. OBJECTIVES: This study determined the dengue vaccine acceptance and the factors associated with acceptance as well as the knowledge, attitudes and practices on dengue fever among parents and caregivers at the PCMC-OPD. METHODS: A hospital-based cross-sectional survey was done at the PCMC-OPD using selfadministered questionnaires regarding the KAP on dengue fever and vaccine acceptance. Multivariate analysis and Spearman’s rank correlation were used to determine predictors of DV acceptance. RESULTS: We found that DV acceptance among the participants was 81.3% (113 out of 139). Educational attainment, employment status, and monthly income are significantly associated with acceptance of dengue vaccine, and being female contributed to high acceptance. DV acceptance was strongly correlated with a lower income class. Educational attainment and employment status seem to affect DV acceptance but are not strong predictors. CONCLUSIONS: The DV acceptance rate of the parents and caregivers of patients consulting at PCMC-OPD was high. The most important factors associated with acceptance are educational attainment, employment status and income class. RECOMMENDATIONS A similar study may be conducted with a larger population to study target populations in the Philippines. This kind of study can be utilized to formulate new strategies addressing the awareness and acceptance of the community for the new dengue vaccine.
Dengue ; Dengue Virus ; Dengue Vaccines ; Philippines

The prevalence of dengue infection poses a great public health concern among people living in tropical and subtropical countries like the Philippines. Just recently in 2019, the Philippines had a dengue virus (DENV) outbreak where nearly half of the mortalities were children between 5 and 9 years of age, and around 73% of confirmed DENV cases were under the age of 19. Children are disproportionately affected and are considered to be highly vulnerable to severe dengue infection compared to adults due to several factors. These include: immunological differences--poorly developed immune systems, making them more susceptible to the virus; smaller body sizes and weight means that they are more likely to experience severe symptoms; and their tendency to play outdoors, make them more exposed to mosquito bites. Hence, this article provides an overview of the current understanding for the vulnerability of children to severe dengue infection compared to adults
dengue virus ; dengue shock syndrome

Dengue virus HA antigens in infected cells from Aedes albopictus clone C6/36 can be produced easily. Their titers that prepared were from 64 to 128 times higher than from baby mouse brain. The method is recommended as an initial step in the concentration of Dengue virus propagated in tissue cullure, as it is simple, rapid and inexpensive
Dengue Virus ; Hemagglutinins

Aedes albopictus C6/36 cells are susceptible to Dengue virus. The C6/36 cell line was infected with type 1 Dengue virus (DEN 1). At different time after these cells infected, morphological observations with electron microscope were conducted with super thin slicing method and the ultrastructural characteristics of DEN 1 virus in infected cells were recorded.
Dengue Virus ; Aedes, Cells

China ; epidemiology ; Dengue ; epidemiology ; Dengue Virus ; Humans

Dengue continues to be a major health threat to Malaysia a century after its first reported outbreak in 1902. Examination of the available outbreak data suggested that a major DF/DHF outbreak occurred in Malaysia in a cyclical pattern of approximately every 8 years. All four dengue virus serotypes are found co-circulating in Malaysia, but after the first and only major outbreak involving DEN-4 in 1960's, only DEN-1, DEN-2 and DEN-3 were associated with DF/DHF outbreaks. It is argued that perhaps the spread of the later dengue virus serotypes followed the pattern of spread of the mosquito vector Aedes aegypti, whereas the former was associated with Aedes albopictus, the outdoor and rural area dwelling mosquito. Estimating from the trend and pattern of dengue and the associated dengue virus serotypes, unless there is a major breakthrough in dengue vaccine development, it is likely that dengue outbreaks will continue to occur in Malaysia throughout the 21st century.
Malaysia ; Dengue ; Pattern ; Dengue Virus ; increasing incidence

Biomarkers ; Biomedical Research ; Dengue ; Dengue Virus ; Humans ; Severe Dengue

Envelope protein (E) of Dengue virus is an important antigen for immune response of Dengue virus, in which domain 3 (E3) encodes important specific epitopes of each serotype of the virus. It would be of a great value to express domain 3 and apply the recombinant proteins for development of the kit for DF/DHF diagnosis. The domain 3 of the gene coding for the envelope protein of dengue virus type 2 was amplified by PCR with primer pair that hangs BamHI and Xhol restriction sites at the 5 prime of the forward and reverse primer, respectively. The template for domain-3 amplification was the gene encoding preM-E protein that had been cloned and sequenced before. The amplified region of the gene was then inserted into the pET-TRX Fusion Expression Vector System with BamHI and Xhol restriction sites and transformed into E. coli BL21 DE3 Star. Recombinant protein D2E3 was expressed in a fusion form with Thioredoxin and His-Tag after induction with ImM IPTG. Recombinant D2E3 was purified with ProBond Nickel-Chelating Resin column (Invitrogen) and the purified recombinant D2E3 protein was used to check the reaction with the antibodies against native dengue antigens in the patient's sera by ELISA, Western blot and Dot blot. The results showed that, recombinant D2E3 protein of Dengue virus type 2 could react specifically with antibodies in the patient's sera.
Dengue Virus ; Escherichia coli ; Antigens