Objective To study the level of serum IL-10 in renal allgraft recipients with stable renal function,acute rejection,infection and chronic allgraft nephropathy(CAN),and to study the level of serum IL-10 in stable renal function recipients with different postoperative time,different dosage of CsA,different rejective frequency in order to find out the value of IL-10 in the follow-up of out-patients. Methods IL-10 were detected randomly by ELISA technique in 127 renal allgraft recipients during the follow-up,and 20 healthy volunteers were enrolled as controls.Results Level of serum IL-10 in stable renal function group was significantly higher than that in rejection group,CAN group and control group(P

Objective To explore the correlation between Helicobacter pylori (Hp) infection and recurrent abdominal pain (RAP) in children over 6 years old and its relative treatment.Me-thods One hundred and eighty children over 6 years old with the diagnosis of RAP from Mar.2007 to Feb.2009 were selected,30 healthy children without RAP were taken as the healthy control group at the same period.14C-urea breath test (14C-UBT) was used to detect whether the patient was infected by Hp.The radical cure of Hp was given to the Hp-positive children with RAP,and the remission rate of children with RAP and the Hp negative rate were observed.Results The positive rate in RAP group was 58.33%,which was higher significantly than that in healthy control group(20.00%)(P0.05).The positive rate of those with and without bad eating habits were 45.56% and 12.78%,and the positive rate of with and without family gastrosis history was 49.44% and 8.89%,which both had significant difference between them(Pa

Objective To investigate the effect of tea polyphenols (TP) on renal damage in rats model induced by D-galactose. Methods Rats were injected with D-galactose (150 mg/kg?d),ip for 8 w,to induce renal damage. From the 3rd week,TP (150,75,37.5 mg/kg?d),aminoguanidine (150 mg/kg) and vitamin E (150 mg/kg) were administered with D-galactose for 6 w. After treatment,fasting blood glucose and 2 h blood glucose in oral glucose tolerance test were measured. The levels of HbA1C and fructosamine in serum,the activity of aldose reductase and content of advanced glycation end products (AGEs) in plasma and in kidney tissues and the activity of SOD,GSH-Px,and the contents of MDA in kidney tissues were measured,and 24h urinary protein,blood urea nitrogen (BUN) and serum creatinine (Cr) were detected. The apoptosis of renal cells were detected by flow cytometer. Results After treatment of D-galactose for 8 w,2h glucose level in oral glucose talerance test was increased significantly,the activity of aldose reductase and the content of AGES were increased significantly in blood. The levels of AGEs and MDA in renal tissues were also enhanced significantly. However,the activities of SOD and GSH-Px decreased. Additionally,the contents of 24h urine protein,BUN,Cr and the apoptotic rate of renal cells were increased significantly. High and middle dose of TP could can decrease the activity of aldose reductase in red blood cells,and inhibit the formation of glycation products in model rats induced by D-galactose. Also,TP could enhance the antioxidative activities and decrease the contents of AGEs and MDA in renal tissues. Mesnwhile,24h urine protein,BUN and Cr and the apoptotic rate of renal cells were increased significantly. Conclusion TP can inhibit glycation reaction induced by D-galactose and then protect renal from damage caused by glycation.

Objective To investigate clinical characteristics of relapsing polychondritis(RP)and to improve early recognition for it.Methods Clinical and laboratory data of 56 patients with RP were analyzed retrospectively.Results Ratio of number of male patients to female ones was 1.2.Age at onset was(46?11)years(ranging from 27 to 71)and average interval between onset and diagnosis was(21? 35)months,(8?6),(16?31)and(29?37)months for patients initial onset with auricle,respiratory tract and joints involved,respectively.Site involved included airway in 40 patients(71.4%),auricle in 32 (57.1%),joints in 32(57.1%),eyes in 27(48.2%),nasal chondritis in 25(44.6%)and inner ear in 13(23.2%).At initial stage of the course,17 patients were misdiagnosed as respiratory infection (30.4%),nine as perichondritis(16.1%),six as pulmonary tuberculosis(10.7%),five as rheumatoid arthritis(8.9%).Seven of 40 patients with airway involvement received metallic stents for their tracheobronchial stenosis.Four patients whose condition never improved after regular therapy all had respiratory involvement.Conclusions Patients of RP with initial onset at non-auricle,non-nasal sites tended to be misdiagnosed.Prevalence of airway involvement was not so low with a poor prognosis in patients of RP.

To investigate the mechanism of proliferation inhibition and apoptosis of K562 leukemia cells by lithium chloride (LiCl), after K562 cells were treated with LiCl (30 mmol/L) cell cycle was examined by flow cytometry (FCM) and the expression of bcr/abl fusion gene mRNA was evaluated by RT-PCR. The intracellular Li(+) concentrations of K562 cells were determined at different time after treated with 30 mmol/L LiCl and the effects of TTX and FSK on intracellular Li(+) concentrations of K562 cells were also detected by atomic absorption spectrometry. The effects of TTX and FSK on LiCl-induced growth inhibition of K562 cells were determined by cell counting in liquid culture. The results showed that LiCl (30 mmol/L) caused a sustained arrest in G(2)/M cell cycle and down-regulated the bcr/abl mRNA expression in K562 cells, the intracellular Li(+) concentration of K562 cells increased at 30 minutes after treated with 30 mmol/L LiCl and reached apex at 2 hours, thereafter, gradually decreased and balanced at 4 hours after the treatment. If either Na(+) channel was pre-blocked with TTX or K(+) channel was pre-blocked with FSK, the intracellular Li(+) concentrations of K562 cells treated with 30 mmol/L LiCl were higher than that in the cells just treated with LiCl without pre-blocking. Furthermore, after pre-blocking either Na(+) channel with TTX or K(+) channel with FSK, the inhibition rate of K562 cell growth by 30 mmol/L LiCl could be increased. It is concluded that the mechanism of proliferation inhibition and apoptosis of K562 leukemia cells induced by LiCl is probably related with the G(2)/M cell cycle arrest, the bcr/abl mRNA expression down-regulation and the status of Na(+), K(+), or Li(+) ion channels on K562 leukemia cells.
Antineoplastic Agents ; pharmacology ; Apoptosis ; drug effects ; Cell Cycle ; drug effects ; Cell Proliferation ; drug effects ; Colforsin ; pharmacology ; Flow Cytometry ; Fusion Proteins, bcr-abl ; genetics ; Gene Expression Regulation, Neoplastic ; drug effects ; Humans ; K562 Cells ; Leukemia ; genetics ; metabolism ; pathology ; Lithium Chloride ; pharmacology ; Potassium Channel Blockers ; pharmacology ; RNA, Messenger ; biosynthesis ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Sodium Channel Blockers ; pharmacology ; Tetrodotoxin ; pharmacology

AIMTo search for more bioactive compounds from the roots of Aconitum coreanum (Lèvl.) Rapaics.

METHODSHigh speed countercurrent chromatography was successfully applied to the separation of alkaloids from Aconitum coreanum. The structures were elucidated by their physicochemical properties and spectroscopic analysis.

RESULTSTwo-phase solvent system composed of CHCl3-CH3OH-0.2 mol x L(-1) HCl (10:3:3, volume ratio) was used in this experiment, eight alkaloids were obtained from the roots of Aconitum coreanum, which were identified as: 2alpha-propionyl-11alpha,13beta-diacetyl-14-hydroxyhetisine (I), Guanfu base P (II), Guanfu base G (III), Guanfu base F (IV), Guanfu base Z (V), Guanfu base O (VI), Guanfu base A (VII), Guanfu base B (VIII).

CONCLUSIONCompound I is a new alkaloid, named Guanfu base R.

Aconitum ; chemistry ; Countercurrent Distribution ; Heterocyclic Compounds, 4 or More Rings ; chemistry ; isolation & purification ; Molecular Conformation ; Molecular Structure ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry

Animals ; Chemical and Drug Induced Liver Injury ; drug therapy ; Liver ; drug effects ; pathology ; Mice ; Silymarin ; pharmacology ; Ursodeoxycholic Acid ; pharmacology

Objective To detect the internal arteriovenous fistulas (AVF) in 73 maintenance hemedialysis patients, as to explore the recirculation, blood flow and cardiac output of the internal AVF and provide basis for the hemodialysis project. Methods Internal AVF of 73 maintenance hemodialysis patients was detected 30 minutes after the initiation and 30 minutes before the end of the hemodialysis. Patients with low blood flow ( AF < 600 ml/min) were followed up. Results Fistula function was successfully monitored in 73patients, in which 1 case ( 1. 4% ) was found to have recirculation rate > 50%. 15 patients (20. 5% ) had AF <600 ml/min and 18 patients (24.7%) had AF > 1 600 ml/min. During 12 months follow up with the 15 patients (AF <600 ml/min), 6 cases (40%) had occlusion, 7 cases (46.7%) maintained unobstructed and 2 cases underwent kidney transplant. There was statistical significance in terms of internal fistula flow, cardiac output, cardiac index and center blood volume between 30 minutes after the initiation and 30 minutes before the end of the hemodialysis. Conclusions AVF detection plays an important role in the monitor of AVF function in hemodialysis patients. The possibility of AVF occlusion increases in patients with lower blood flow. The recirculation rate is low when AVF obtains enough blood flow and the internal fistula interval is not a major factor.

Objective To study the effect of continuous quality improvement on the health education of treatment of tuberculosis in thoracic surgical.Methods Totals of 278 cases between January and December in 2010 received the conventional health education as the control group,and 297 cases between January and December in 2011 as observation group received for continued quality improved,through change shifts mode,provides personalized health education content,strengthening specialist knowledge and the communication skills training,take rectification measures and evaluation effect.After one year,patients' health status,complication incidence,satisfaction of nursing quality were evaluated and compared between two groups.Results The achieved rate of health status (93.9％ vs 87.1％) and satisfaction of nursing quality (94.6％ vs 86.3％) in observation group were significantly better than that in control group (x2 =8.01,P ＜ 0.01),as well as the complication incidence (12.1％ vs 20.1％,x2 =6.88,P ＜ 0.01).Conclusions Continuous quality improvement not only has a good role in promoting postoperative health status,and reduce the incidence of postoperative complications,thus can improve the prognosis of patients with quality of care and quality of life satisfaction.

Objective] To analyze the effect of enhanced immunization for school-age children in Hongkou District of Shanghai in immunology and epidemiology . [ Methods] One month after completing immunization, the blood antibody titers were measured by ELISA method , and compared with the those of non-enhanced immunization , for epidemiological survey were collected history of measles immunization and close contact with the measles . [ Results] It could not be thought that strengthening immunization im-proved antibody positivity rate , the protection rate , and geometric antibody average concentrations .There was not statistical difference in antibody levels between measles vaccination 2 times and 3 -4 times. [ Conclusion] In the regions where routine immunization rates reach a high level , strengthening immuni-zation done on large scale is a waste of vaccine resources , human resources and financial resources , and leak re-vaccination should be done as a cost-effective preventive measure .