1.Effects of moderate exercise on lymphocyte number and Th1/Th2 balance in rats under chronic psychological stress
Yan ZHAO ; Chun WANG ; Yu WANG
Chinese Journal of Tissue Engineering Research 2009;13(50):9933-9936
BACKGROUND: Previous studies have proved that stress can affect immunity function and disturb homeostasis of bodies. Moderate exercise can alleviate the psychological stress reaction to some degree.OBJECTIVE: To observe the effects of moderate exercise on lymphocyte number in spleen and mesenteric lymph nod,interferon-y (IFN-γ) and interleukine-4(IL-4) level in serum, as well as Th1/Th2 balance in rats under chronic psychological stress.DESIGN, TIME AND SETTING: A randomized control animal experiment was carried out in the laboratory of Sports Human Science in Chengdu Sports University from May to October in 2007.MATERIALS: A total of 40 healthy male SD rats were divided by random digits table into 4 groups: control group, psychological stress group, exercise group and exercise+psychological stress group, with 10 ones in each group.METHODS: Before experiment, rats in exercise group and exercise+psychological stress group underwent 3 days of adaptive swimming training, 30 min/(t·d). During experiment, they were made swimming with no weight loading for 8 weeks, 60 min/(t·d).From day 2 post exercise on, rats in exercise+psychological stress group began to undergo the 21 days of chronic restraint stress,6 hours per day. At the same time, the chronic restraint stress models were established in the psychological stress group. Rats in the exercise group were fed for 22 days since the 8~(th) day of exercise ended. Rats in the control group were only fed without any intervention.MAIN OUTCOME MEASURES: The lymphocyte number in spleen and mesenteric lymph node was measured, and ELISA was employed to measure IFN-y and IL-4 levels as well as the ratio of IFN-y/IL-4 in serum.RESULTS: Compared with the control group, the lymphocyte number, the level of IFN-y and the ratio of IFN-y/IL-4 were significantly decreased in psychological stress group (P < 0.01 ), and the Thlf'rh2 balance was broken. Compared with the psychological group, the exercise+psychological stress group had got significantly higher lymphocyte number, IFN-γ level and ratio of IFN-γ/IL-4 (P<0.05). The lymphocyte number in spleen and mesenteric lymph node in the exercise group showed not a difference of significance compared with the control group, but a tendency towards going up. There was no difference of significance in IFN-γ level and ratio of IFN-γ/IL-4 among the exercise+psychological stress group, the exercise group and the control group.CONCLUSION: Moderate exercise can increase lymphocyte number in spleen and mesenteric lymph node significantly,up-regulate the IFN-γ level so as to regulate the disbalance of Th1/Th2, and maintain immunity homeostasis.
2.Efficacy and safety of phosphoric acid phosphate combined metformin in the treatment of diagnosis of type 2 diabetes mellitus
Haiyan YAN ; Ming ZHAO ; Chun LUO
Chinese Journal of Biochemical Pharmaceutics 2017;37(4):198-200
Objective To evaluate the efficacy and safety of phosphoric acid phosphate combined metformin in the treatment of diagnosis of type 2 diabetes mellitus.Methods A total of 86 cases with diagnosis of type 2 diabetes mellitus included in this study were dividing into control group and experiment group with each 43 cases.The patients in the both groups were give regular treatment.Patients in the control group were given metformin hydrochloride sustained release tablets 500mg by oral administration with 3 times a day and patients in the experiment group revieved phosphoric acid phosphate by oral administration with once a day.A cycle of 2 groups of patients were 28d,a total of 3 cycles of treatment.The ADP,APAF1,D25-(OH)D3),CysC and adverse drug reactions were compared between the two groups.Results The clinical efficacy in experiment group was 93.02%,which significant higher than that in control group 76.74%(P<0.05).The serum level of ADP,APAF1 and CysC decreased after treatment with experiment group much lower than the control group(P<0.05).The serum D25-(OH)D3l elevated in the two groups with experiment group much higher than the control group(P<0.05).The adverse drug reactions was not statistically different between the two groups.Conclusion Phosphoric acid phosphate combined metformin in the treatment of diagnosis of type 2 diabetes mellitus was effective with high safety.
3.Acupuncture for acute ankle sprain.
Chinese Acupuncture & Moxibustion 2011;31(6):486-486
Acupuncture Therapy
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Adolescent
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Adult
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Ankle Injuries
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therapy
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Humans
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Male
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Middle Aged
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Sprains and Strains
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therapy
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Young Adult
5.Influence of nerve growth factor and aminogunidine on visual evoked potential in diabetic rats
Yan-Zhi SANG ; Xin LIU ; Lin LIU ; Chun-Yan ZHAO ;
Academic Journal of Second Military Medical University 1985;0(06):-
Objective:To observe the changes of visual evoked potential(VEP)in diabetic rats and the influence of nerve growth factor(NGF)and aminoguanidine(AG)on VEP.Methods:Diabetes was induced in adult male Wistar rats with streptozotocin(STZ).Rats were divided into normal control group(CON),diabetes model group(DM).NGF-treated group(D +N)and AG-treated group(D+A).VEP was measured during the 3~(rd)month,6~(th)month,9~(th)month,and 12~(th)month.Results: Compared with the CON group,all rest groups had longer latencies and lower amplitudes(P
6.Killing effect of IL-12-activated A-NK cells on human hepatocellular carcinoma HepG-2 cells in vitro.
Zhi-Hua WANG ; Dong-Lu ZHAO ; Chun-Yan ZHANG
Chinese Journal of Oncology 2007;29(6):423-424
Animals
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Carcinoma, Hepatocellular
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blood supply
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pathology
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therapy
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Cell Line, Tumor
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Cells, Cultured
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Cytotoxicity, Immunologic
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immunology
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Female
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Humans
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Interleukin-12
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pharmacology
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Interleukin-2
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pharmacology
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Killer Cells, Natural
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cytology
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drug effects
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immunology
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Liver Neoplasms, Experimental
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blood supply
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pathology
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therapy
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Lymphocyte Activation
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drug effects
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immunology
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Microcirculation
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drug effects
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Random Allocation
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Xenograft Model Antitumor Assays
7.System for monitoring patients in hospital based on Zigbee
Yuan LIU ; Chun LI ; Yan ZHAO ; Yuan YAO
Chinese Medical Equipment Journal 2003;0(11):-
Objective To design a system for real-time monitoring patients Based on Zigbee.Methods A wireless monitoring system was developed,the hardware of which was based on micro-cpu of MCS08 and Zigbee chip MC13192.Results Through this wireless system,doctors and nurses could know the location and physical situation of patients in real time so that the patients who were critically ill could be treated in time and the work of doctors and nurses could be relieved.Conclusion The system for monitoring patients in hospital based on Zigbee meets the nosocomial condition and can raise curative efficiency.
10.Application of diffusion-weighted imaging in the diagnosis and treatment of cervical cancer
Chun FU ; Xiaoyan FENG ; Xiangquan WANG ; Yan ZHAO
Journal of International Oncology 2012;39(6):449-452
Diffusion-weighted imaging (DWI) is sensitive to identification of cervical lesions and lymph node metastasis.DWI can be used to predict and evaluate the therapeutic effect of cervical cancer.Using magnetic resonance imaging and DWI scans in the process of diagnosis and treatment of cervical cancer,which may contribute to the personalized treatment program and improve prognosis for patients.