2.Clinical significance of the anticardiolipin antibodies in patients with hemorrhagic fever with renal syn-drome
Jin-Chuan HU ; Yan YAN ; Dan YANG ; Qi YANG ; Ming ZHOU ; Lu MA ;
Chinese Journal of Infectious Diseases 2000;0(02):-
Objective To investigate the clinical significance of anticardiolipin antibodies (ACA)in patients with hemorrhagic fever with renal syndrome(HFRS).Methods The serum ACA- IgM and ACA-IgG in 175 cases of renal diseases and 50 healthy cases were detected by enzyme-linked immunosorbent assay(ELISA). The positive rates of ACA were calculated and compared among different groups.Results The positive rates of ACA-IgM and ACA-IgG in control group were both 0 (0/50),while they were 85.2%(46/54)and 24.1%(13/54)respectively in patients with HERS. 25.0%(13/52)and 21.2%(11/52)respectively in patients with lupus nephritis and 13.0%(9/69) and 8.7%(6/69)respectively in patients with nephrotic syndrome.The poshive rate of ACA lgM in patients with HFRS was higher than that in control group,lupus nephritis group and nephritic syn- drome group(P
5.Clinical characteristics and SLC12A3 gene mutation profile of Gitelman syndrome: One case report
Wan LU ; Ying GUO ; Chang NI ; Jin LI ; Xian LIN ; Li YAN ; Chuan YANG
Chinese Journal of Endocrinology and Metabolism 2015;31(5):438-442
Objective To describe the clinical characteristics of a patient with Gitelman syndrome,and to identify the associated SLC12A3 gene mutations.Methods A suspected case of teenager-onset Gitelman syndrome was observed in our hospital.It was further confirmed by clinical manifestations and auxiliary examination.In addition,direct sequencing for the exons of SLC12A3 gene and CLCNKB gene region was conducted to identify the probable disease-associated mutations.Results The case showed characteristics of hypokalemia,hypomagnesemia,and low level of urinary calcium and onset by age of 18.By excluding the possibilities of long-term use of thiazide diuretics,laxatives,chronic vomiting and diarrhea,he was finally diagnosed as a case of Gitelman syndrome.Furthermore,by Sanger direct sequencing,2 coding variations were identified in SLC12A3 gene region,including T304M and L488P.L488P was a new heterozygous mutation.Conclusion Detection of SLC12A3 gene mutation could facilitate the diagnosis of Gitelman syndrome and improve prognosis.
6.Colorectal Cancer Screening Using Immunochemical Fecal Occult Blood Test
Mun Chieng Tan ; Ooi Chuan Ng ; Ray Yee Paul Yap ; Yan Pan ; Jin Yu Chieng
Malaysian Journal of Public Health Medicine 2017;17(1):33-37
Fecal occult blood test (FOBT) screening has been shown to decrease the incidence and mortality of colorectal cancer
(CRC). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the
immunochemical fecal occult blood test (i-FOBT) in diagnosing CRC were assessed among the patients in a tertiary
referral hospital in Malaysia. A total sample of 814 patients aged 16 to 85 years old who performed i-FOBT and
endoscopic screenings was obtained. The patients were recruited for a retrospective investigation. Sensitivity,
specificity, PPV, and NPV were derived for the CRC screenees. Out of the 814 patients screened using i-FOBT, half of
them were above 59 years old (49.6%), and 36% had positive i-FOBT. Gender distribution was almost equal, where 53.4%
of the patients were female, and 46.6% were male. Majority of the patients were Malays (56.6%), followed by Chinese
(24.0%), Indians (16.5%), and others (2.9%). Among the 71 patients referred for colonoscopy, 57.7% and 42.3%
corresponded to positive and negative i-FOBT cases, respectively. Polyps were found to be most common among the
patients (25.6%), 7.0% were found positive for invasive CRC, and 35.2% had normal colonoscopic findings. There was a
significant association between colonoscopic finding and positive i-FOBT (p=0.001). The sensitivity, specificity, PPV, and
NPV for CRC detection were 66.7%, 43.0%, 9.8%, and 93.3%, respectively. The results indicate that i-FOBT is a useful tool
in the detection of abnormalities in the lower gastrointestinal tract and therefore serves as a cornerstone for potential
large-scale screening programmes.
7.Current status of iodine deficiency disorders in Henan Province in 2011
He-ming, ZHENG ; Xiao-feng, LI ; Jin, YANG ; Chuan-gang, WANG ; Ning, SUN ; Yan-li, WANG
Chinese Journal of Endemiology 2013;32(5):526-528
Objective To analyze the present situation of iodine deficiency disorders (IDD) in Henan Province,and to promote implementation of sustainable control strategies.Methods In 2011,a stratified proportion to population probability sampling (PPS) method was used to survey 1200 children aged 8 to 10 in 30 counties of the province.One primary school was selected in each chosen county.Goiter,intelligence quotient (IQ),urinary iodine and salt iodine level were studied.Meanwhile,12 families per capita salt intake was investigated.In each school,30 5th-grade students and 30 pregnant and lactating women in the school townships and adjacent neighboring townships were selected to carry out questionnaire survey on health education with unified papers.Results ①The goiter rate of children aged 8 to 10 by B ultrasound was 4.5% (54/1201) ; the IQ of 1080 children was 107.75 ± 16.81 ; median urinary iodine level of 358 children was 201.4 μg/L.②The median of salt iodine content was 28.6 mg/kg,the coverage rate of iodized salt was 98.8% (1186/1200),and qualified rate of iodized salt was 93.0% (1116/1200).③The residents average daily salt intake was 10.5 g.④Average score of the questionnaire survey of 1084 5th-grade students was 4.2 points.Average score of 961 housewives was 4.4 points.Conclusions Various technical indicators show that IDD is in a sustained elimination state in Henan Province.Strengthen health education,enhance public awareness of disease prevention is still the important work ahead.
8.External quality control of iodine deficiency disorders laboratory in Henan province from 1999 to 2011
Chuan-gang, WANG ; Ning, SUN ; Yan-li, WANG ; Xiao-feng, LI ; Jin, YANG ; He-ming, ZHENG
Chinese Journal of Endemiology 2012;31(6):687-689
Objective To analyze the examination results of external quality assessment(EQA) at all levels of iodine deficiency disorders(IDD) laboratories in Henan province and the network operation to further standardize and improve the laboratory,and to provide reliable laboratory quality assurance for surveillance and control of IDD.Methods The examination results of EQA at all levels of IDD laboratories in Henan province were statistically analyzed in accordance with the National Reference Laboratory (NRL) of IDD (1999-201 1).Results The survey results showed that the provincial level laboratory was all qualified in testing urinary iodine and salt iodine in the past 13 years.In prefectural level,the laboratory response rates were 100.0%,and through participation in EQA,laboratory capacity had been significantly increased and stabilized.From 1999 to 2001,the passing rate of check up of urinary iodine was 22.2% (4/18),72.2% (13/18),94.4% (17/18),respectively,and the rate was stable at 100.0%(18/18) from 2002 to 2011 except 94.4% (17/18) in 2003.Since 2000,the prefectural level laboratory began to take part in the salt iodine EQA,and the laboratory response rate was 100.0% (18/18) from 2000 to 2011.Except 88.9%(16/18) in 2003,the passing rate of check up of urinary iodine was 100.0%(18/18)from 2000 to 2011.In 2003 and 2004,6 to 7 county-level laboratories participated in the EQA of urinary iodine in Zhengzhou city,respectively,and all qualified.The number of county-level laboratories that participated in the salt iodine quality control network increased from 29 in 1999 to 148 in 2011.Response rate was 94.4%(68/72),96.7%(58/60) and 92.3%(144/156)in 2003,2006 and 2007,respectively,and the rate remained stable at 100.0% in the remaining 10 years.In 1999,the passing rate was 69.0% (20/29),then increased significantly,except 86.7% (26/30) in 2001 and 84.6%(132/156) in 2007,the rates were all above 90.0% in other years,especially in 2000 and 2009,the passing rates were both 100.0%.Conclusions The accuracy of test results of external quality controls and the normal operation of the network at all levels of laboratories is closely related to the IDD laboratory conditions and detection techniques.
9.Expression and procoagulant activity of phosphatidylserine on the normal blood cells.
Shu-chuan LIU ; Jin ZHOU ; Jia-lan SHI ; Yan-hua SU
Chinese Journal of Hematology 2011;32(12):858-862
OBJECTIVETo investigate the expression and procoagulant activity of phosphatidylserine (PS) on the normal peripheral blood cells of adults.
METHODSNormal peripheral blood samples were collected from 10 healthy volunteers (5 ml from each volunteer), platelets, neutrophils, lymphocytes and erythrocytes were isolated. The expression and procoagulant activity of PS on normal blood cells were identified by flow cytometry, inhibition test with lactadherin as PS probe and coagulation anticoagulant, respectively.
RESULTSThere was PS expression on a few normal blood cells (9.1%, 5.4%, 3.9% and 3.2% in platelets, neutrophils, lymphocytes and erythrocytes, respectively). The PS on these normal blood cells in vitro showed significant procoagulant activity. The plasma recalcification time was shortened by 47%, 36.5%, 25% and 12.5% by platelets, neutrophils, lymphocytes and erythrocytes, respectively; the formation of factor Xa (through both intrinsic and extrinsic pathways) and thrombin was also increased by 13% - 26% by platelets, neutrophils, lymphocytes and erythrocytes, respectively.
CONCLUSIONThe PS on normal blood cells in vivo may play a crucial role in the coagulation cascade.
Adult ; Blood Cells ; metabolism ; physiology ; Blood Coagulation Tests ; Female ; Flow Cytometry ; Humans ; Male ; Phosphatidylserines ; metabolism
10.Cloning and expressing of cellulase gene (cbh2) from thermophilic fungi Chaetomium thermophilum CT2.
Shou-An LIU ; Duo-Chuan LI ; Shi-Jin E ; Yan ZHANG
Chinese Journal of Biotechnology 2005;21(6):892-899
Chaetomium thermophilum CT2 can produce extracellular cellulase with industrial value. We designed two degenerate primers to amplify catalytic domain sequence of cellobiohydrolase II ( CBH II). Full length of cDNA was obtained by rapid amplification of cDNA ends technologies. DNA sequencing revealed that cbh2 has an open reading frame of 1428bp, which encodes a putative polypeptide of 476 amino acids. The deduced amino acid sequence shows that the predicted molecular mass is 53 kD and the cbh2 consists of a fungal-type carbohydrate binding domain (CBD) separated from a catalytic domain by a linker region rich in proline/serine/threonine. PCR product consisting of the entire CBH II coding region without its signal sequences was cloned into the yeast secretive plasmid pPIC9K, which was then transformed into Pichia pastoris GS115. Highly efficient production of the cellobiohydrolase II was achieved in P. pastoris under the control of the AOX1 promoter, and the expressing level was 1.2 mg/mL by small-scale culturing. The recombinant cellobiohydrolase II was purified by using ammonium sulfate fraction, DEAE-Sepharose Fast flow chromatography. A molecular mass of the purified enzyme is 67 kD determined by SDS-PAGE and this is similar to the native cellobiohydrolase II purified from C. thermophilum CT2. The recombinant enzyme exhibited optimum catalytic activity at pH 4.0 and 50 degrees C respectively. It was thermostable at 50 degrees C and retained 50% of its original activity after 30 min at 70 d degrees C . The high level of fully active recombinant cellobiohydrolase II got from P. pastoris makes this expression system attractive for fermentor and industrial applications.
Amino Acid Sequence
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Base Sequence
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Cellulose 1,4-beta-Cellobiosidase
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biosynthesis
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genetics
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Chaetomium
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enzymology
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genetics
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Cloning, Molecular
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DNA, Complementary
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genetics
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Fungal Proteins
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biosynthesis
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genetics
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Molecular Sequence Data
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Open Reading Frames
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genetics
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Pichia
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genetics
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metabolism
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Recombinant Proteins
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biosynthesis
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genetics