Objective To compare the clinical efficacy of different internal fixation methods in the treatment of intertrochanteric fractures.Methods he clinical data of 270 elderly patients with intertrochanteric fracture were retrospectively analyzed.According to the different internal fixation methods,the patients were divided into three groups,namely anti-rotation intramedullary nail(PFNA) group,dynamic hip screw(DHS) group and anatomic locking plate (LCP) group,90 cases in each group.The incision length,operative time,blood loss,hospital stay,postoperative weight-bearing time,postoperative bleeding and postoperative complications were compared among the three groups.The fracture healing was compared among the three groups during follow-up.Results The incision length of PFNA group compared with the DHS group (t =11.248,P =0.000) and the LCP group (t =12.371,P =0.000),the differences were significant.The operative time of PFNA group compared with the DHS group (t =15.048,P =0.000) and the LCP group(t =12.822,P =0.000),the differences were significant.The blood loss of PFNA group compared with the DHS group (t =5.803,P =0.000) and the LCP group (t =10.879,P =0.000),the differences were significant.The incidence rates of complications in PFNA group,DHS group,LCP group were 3.33％ (3/90),10.00％ (9/90),13.33％ (12/90),respectively,and theincidence rate of PFNA group was lower than the other two groups (x2 =5.268,P =0.026).The average period of fracture healing in PFNA group compared with the DHS group (t =10.217,P =0.000) and the LCP group (t =4.530,P =0.000),the differences were significant.Conclusion PFNA in the treatment of senile intertrochanteric fractures has advantages such as less trauma,shorter operation time,less blood loss,fewer complications,faster recovery,it is worthy of clinical use.

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Myocardial Infarction ; drug therapy ; Myocardial Reperfusion Injury ; prevention & control ; Phytotherapy ; Thrombolytic Therapy ; Urokinase-Type Plasminogen Activator ; therapeutic use

Objective To explore the effect of high dose γ-irradiation on the phenotype and function of dendritic cells (DCs) and to study the underlying mechanism of irradiation-induced immunosuppression. Methods GM-CSF and IL-4 were used to generate DCs, which were then subjected to 0, 10, 20, and 30 Gy γ irradiation. After 24 h irradiation DCs were treated with lipopolysaccharide for maturation. Then Transwell assay was used to determine the migration capacity of DCs, flow cytometry was used to detect the surface molecules on DCs (CD80, CD86, MHC-II, and CCR7). Cytokines secretion (IL-6, IL-10, and PGE2) was determined by ELISA. Resulls High dose γ-irradiation showed no influence on the phenotypes of DCs, but inhibited the migration of DCs towards CCL19. Moreover, the irradiation down-regulated CCR7 expression and decreased the secretion of IL-6, IL-10, and PGE2. Conclusion High dose γ-irradiation can inhibit DC migration by reducing CCR7 and induaing apoptoais. Moreover, it can also reduce the cytokine secretion, which provide a theoretical base for irradiation-induced immune suppression.

Objective: To investigate the chemical constituents of 80% ETOH extract from rhizome of Belamcanda chinensis (L.) DC. Methods: The constituents were isolated and purified by chromatography on silica gel, Sephadex LH-20 and HPLC; the structures of the compounds were determined by MS and NMR spectral analysis. Results: Ten constituents were identified from rhizome of Belamcanda chinensis (L.) DC, including tectoridin (I), acetovanillone (II), 4-hydroxy-acetophenone (III), β-sitosterol (IV), β-daucosterol (V), 5, 7, 4′-trihydroxyl-3′, 5′-dimethoxyflavone (VI), luteolin (VII), apigenin (VIII), 5,7,4′-trihydroxyflavanones (IX), and isorhamnetin (X). Conclusion: Compounds VI, VI, VIII, and IX have been isolated from rhizome of Belamcanda chinensis for the first time.

Child ; Humans ; Kidney Diseases ; etiology ; pathology ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; complications ; diagnosis

Wear of polyethylene (PE) tibial inserts is a significant cause of implant failure of total knee arthroplasty (TKA). PE inserts wear measurement and evaluation is the key in TKA researches. There are many methods to measure insert wear. Qualitative methods such as observation are used to determine the wear and its type. Quantitative methods such as gravimetric analysis, coordinate measuring machines (CMM) and micro-computed tomography (micro-CT) are used to measure the mass, volume and geometry of wear. In this paper, the principle, characteristics and research progress of main insert wear evaluation method were introduced and the problems and disadvantages were analyzed.
Arthroplasty, Replacement, Knee ; Humans ; Knee Prosthesis ; Polyethylene ; Prosthesis Design ; Prosthesis Failure ; Tibia ; Tomography, X-Ray Computed ; X-Ray Microtomography

0.05)respectively.[Conclusion]The technique of radiographic assessment to define the anteromedial OA of the knee is not easy to perform,but it can be used,by well trained doctors,in the most knee OA cases with high accuracy.The MR imaging gives more information about cartilage defects and can be used in some doubted cases.

Abstract: Objective　To investigate the composition and diversity of midgut microbial community of Haemaphysalis longicornis infected with severe fever with thrombocytopenia syndrome virus (SFTSV)． Methods　The midgut DNA of three group Haemaphysalis longicornis infected with SFTSV was extracted, and the 16S rDNA gene of the sample was sequenced by HiSeq platform. The composition and diversity of endosymbiotic microbial community were clarified by OTU cluster analysis and alpha diversity analysis. Results　The midgut microbial clusters of the three groups infected with SFTSV were 143, 113, 163 OTUs respectively; the sparsity curve and abundance grade curve showed that the data had sufficient sequencing depth, and the midgut of Haemaphysalis longicornis infected with SFTSV was rich in microbial composition, but the species distribution was uneven. The analysis of microbial community composition showed that Proteobacteria, Firmicutes and Actinobacteria were the main dominant bacteria at the phyla level. At the class level, Gammaproteobacteria, Bacilli, Betaproteobacteria and Actinomycetia were the main dominant bacteria. At the order level, Legionellales, Bacillales, Burkholderiales and Actinomycetales were the main dominant orders. At the family level, Coxiellaceae, Bacillaceae, Moraxellaceae and Rhodococcaceae were the main dominant families. At the genus level, the relative abundance of Coxiella was the highest, followed by Aeribaillus and Azonexus. Alpha diversity analysis showed that the average Shannon index was 139.67, the average Simpson index was 0.48, the average Chao index was 145.06, and the average ACE index was 147.11. Conclusions　The species diversity of intestinal microorganisms in Haemaphysalis longicornis infected with SFTSV is rich. The results provide a basis for further exploring the interaction between intestinal microbes of Haemaphysalis longicornis and SFTSV and developing new ideas for the prevention and control of ticks and tick-borne diseases.