We report a case of acute septic arthritis of the hip in a child due to H. influenzae with review of the problems encountered. A one year and 9 months-old male child was admitted because of fever, limping and restlessness of 48-hour duration. Under the impression of septic arthritis of the right hip, blind broad-spectrum antibiotic regimen was initially instituted. Fever subsided immediately and two blood cultures yielded no growth, as did joint tapping. But fever recurred on the 8th day of admission. MRI demonstrated fluid collection in the right hip. Arthrotomy yielded thin pus. The antibiotic regimen was shifted to vancomycin, according to the culture report of S. aureus. However, spiking fever continued. On the 4th week of admission, recheck MRI demonstrated fluid collection in and out of the hip joint. A second arthrotomy yielded frank pus. H. influenzae was reported in pus culture for the first time, being susceptive only to chloramphenicol. Fever was down to normal temperature after injection of urfamycin. Radiograph on the 17th day after the 2nd arthrotomy revealed posterior dislocation of the right hip. MRI demonstrated granulation tissue within the acetabulum. Open reduction confirmed granulation tissue without pus. This case suggests: 1. Routine blind antibiotic regimen for gram-positive and negative organisms in the initial phase of treatment may fail in the rare case of H. influenzae. This may lead the surgeon to miss the optimum time of surgical intervention despite early diagnosis, 2. Arthrotomy may fail if not followed by antibiotics sensitive to the pathogen. 3. Delayed pathologic dislocation may ensue due to invasion of granulation tissue.
Acetabulum ; Anti-Bacterial Agents ; Arthritis, Infectious ; Child* ; Chloramphenicol ; Dislocations ; Early Diagnosis ; Fever ; Granulation Tissue ; Hip Joint ; Hip* ; Humans ; Infant ; Influenza, Human ; Joints ; Magnetic Resonance Imaging ; Male ; Psychomotor Agitation ; Suppuration ; Thiamphenicol ; Vancomycin

The study consist of 134 neonatal pneumonia who under 28 days born addmitted to The Neonatal department of National Institute of Peadiatrics from December 2001 to November 2002. The out comes showed that: Positive rate: 71.15%, negative rate: 29.85%. Common isolated bacteria were negative Gram. Ampicillin, gentamycin, chloramphenicol bactrim have resistance antibiotic. The recovered treatment result: 90.3%; mortality: 9.7% in which 61.2% are fully recovered without change other antibiotic. The average treatment duration by antibiotic:13.2%
Pneumonia ; Anti-Bacterial Agents ; Chloramphenicol

This study was aimed to assess the dissolution of 4 chloramphenicol capsule products of 4 Vietnamese pharmaceutical enterprises. The results showed that most of them have a very low drug release degree. The cause may be resulted from making capsule without making particles, too raw powder, using too much insoluble excipient, too high rate of insoluble excipient. The dissolution test should be considered an item of chloramphenicol capsule specification
Chloramphenicol ; Adjuvants, Pharmaceutic ; Capsules ; drugs

Background/Objective: Haemophilus influenzae type b remains to be a significant etiology of invasive infections specially in children two months to five years old without Hib vaccination. This study was performed to compare the cure rates of ampicillin, chloramphenicol, ampicillin-chloramphenicol combination and third generation cephalosporins as initial antibiotic treatments for documented invasive Hib infections. This study may assist in formulating recommendations on empiric antimicrobial therapy. Methods: Charts of patients with invasive Hib disease confirmed either by blood culture, CSF culture and/or latex agglutination test from January 1991 to August 2010 were reviewed. Cases were classified into four groups depending on the initial antibiotic given upon admission. The four groups were compared and analyzed in terms of cure rates. Results The disease occurred predominantly in children less than two years old. Males were more frequently affected than females. All subjects were not given Hib vaccination. Cure rates were significantly different between ampicillin (33%) and chloramphenicol (89%) groups (p=0.017), and between chloramphenicol (89%) and ampicillin-chloramphenicol (39%) groups (p=0.008). However, cure rates were not significantly different when third generation cephalosporin group (62%) was compared to the other treatment groups (p>0.05). Resistance of Hib was 31% to ampicillin, while <10% to chloramphenicol and third generation cephalosporins. Conclusion: Chloramphenicol is an excellent drug for empiric therapy in highly suspected or proven cases of invasive Hib disease.
Ampicillin ; Chloramphenicol ; Third Generation Cephalosporins ; Haemophilus influenzae

Seventy Escherichia coli isolates recovered from diseasedchickens diagnosed with colibacillosis in Henan Province,China, between 2004 and 2005 were characterized forantimicrobial susceptibility profiles via a broth doublingdilution method. Overall, the isolates displayed resistanceto trimethoprim-sulfamethoxazole (100%), oxytetracycline(100%), ampicillin (83%), enrofloxacin (83%), and ciprofloxacin(81%), respectively. Among the phenicols, resistance wasapproximately 79% and 29% for chloramphenicol andflorfenicol, respectively. Molecular detection revealed thatthe incidence rates of the floR, cmlA, cat1, cat2 and cat3were 29, 31, 16, 13, and 0%, respectively. Additionally,10% of the isolates were positive for both floR and cmlA.As these antimicrobial agents may potentially inducecross-resistance between animal and human bacterialpathogens, their prudent use in veterinary medicine ishighly recommended.
Animals ; Anti-Bacterial Agents/*pharmacology ; *Chickens ; China/epidemiology ; Chloramphenicol/pharmacology ; DNA, Bacterial/chemistry/genetics ; Drug Resistance, Multiple, Bacterial ; Escherichia coli/*drug effects/growth & development ; Escherichia coli Infections/epidemiology/microbiology/*veterinary ; Microbial Sensitivity Tests/veterinary ; Polymerase Chain Reaction/veterinary ; Poultry Diseases/epidemiology/*microbiology ; Thiamphenicol/analogs & derivatives/pharmacology

Protermycin, the first molecular synthetic of tetracycline and chloramphenicol, has been given trial in 28 patients: acute gonococcal urethritis 10, nongonococcal urethritis 7, acute cystitis 6, chancroid 1 and postoperative cases 4. The doses ranged from 300 to 600 me daily. From this study, it was found that Protermycin proved itself to be most effective in nongonococcal urethritis which could not be treated satisfactorily by other antibiotics. Of 28 cases, 25 showed good result. The remaining 3 cases failed to respond.
Anti-Bacterial Agents ; Chancroid ; Chloramphenicol ; Cystitis ; Humans ; Tetracycline ; Urethritis

Microorganisms are the dominant players driving the degradation and transformation of chloramphenicol (CAP) in the environment. However, little bacterial strains are able to efficiently degrade and mineralize CAP, and the CAP degrading pathways mediated by oxidative reactions remain unclear. In this study, a highly efficient CAP-degrading microbial consortium, which mainly consists of Rhodococcus (relative abundance >70%), was obtained through an enrichment process using CAP-contaminated activated sludge as the inoculum. A bacterial strain CAP-2 capable of efficiently degrading CAP was isolated from the consortium and identified as Rhodococcus sp. by 16S rRNA gene analysis. Strain CAP-2 can efficiently degrade CAP under different nutrient conditions. Based on the biotransformation characteristics of the detected metabolite p-nitrobenzoic acid and the reported metabolites p-nitrobenzaldehyde and protocatechuate by strain CAP-2, a new oxidative pathway for the degradation of CAP was proposed. The side chain of CAP was oxidized and broken to generate p-nitrobenzaldehyde, which was further oxidized to p-nitrobenzoic acid. Strain CAP-2 can be used to further study the molecular mechanism of CAP catabolism, and has the potential to be used in in situ bioremediation of CAP-contaminated environment.
Biodegradation, Environmental ; Chloramphenicol ; RNA, Ribosomal, 16S/genetics* ; Rhodococcus/genetics* ; Sewage

Haemophilus influenzae type b (Hib) is a major cause of meningitis and pneumonia in children. In Papua New Guinea (PNG) more than 20% of Hib are now resistant to chloramphenicol, and resistant Hib meningitis treated with chloramphenicol results in certain death or severe brain injury. Third-generation cephalosporins are a therapeutic option but are very expensive, while the Haemophilus influenzae type b conjugate vaccine would provide effective prevention. In a province of 380,000 people, using ceftriaxone as standard treatment for meningitis in all health facilities would only save an estimated 8 more lives per year than using chloramphenicol, and cost US dollars 1514 per additional life saved. Introduction of Hib vaccine would save, each year, 61 more lives than using chloramphenicol and 53 more lives than using ceftriaxone for meningitis treatment. The cost of a vaccination strategy for Hib meningitis would be US dollars 1216 for each of the 61 additional lives saved. Hib vaccine would be by far the most effective intervention to reduce mortality and severe neurological disability from Hib meningitis in PNG. Nationwide introduction of Hib vaccine is urgently needed, as antibiotics are now less effective in this disease than ever before.
Haemophilus influenzae type b bacteria ; Meningitis ; Vaccines ; Chloramphenicol ; Prevention

Cancer resistance to treatment ; Peninsular Malaysia ; Tetracyclines ; Salmonella typhi ; Chloramphenicol

Tsutsugamushi disease (scrub typhus) is a rickettsian febrile disease which is seldom found in pregnancy. A 28-year-old woman was infected with scrub typhus at the 22th week of gestation which was diagnosed on the basis of clinical features. A serology for Tsutsugamushi was negative at that time. Therapy with tetracycline (doxycycline) or chloramphenicol is currently recommended for the treatment. However these drugs which are class D drugs according to the USA FDA, and Fetal Risk Summary, should not be used to treat pregnant women. Recently azithromycin, a new macrolide antibiotic, has been proven to be more effective than doxycycline. Moreover, there is no evidence that azithromycin causes harm to the developing fetus or to children. Here we describe a pregnant patient with Tsutsugamushi disease who was treated successfully with azithromycin without complications.
Adult ; Azithromycin ; Child ; Chloramphenicol ; Doxycycline ; Female ; Fetus ; Humans ; Pregnancy ; Pregnant Women* ; Scrub Typhus* ; Tetracycline