Burns ; pathology ; therapy ; Humans ; Prognosis ; Treatment Outcome ; Wound Healing

Objective To investigate the role of steroids on the intracellular signal transduction mechanism of nongenomic effects for L 929 cells to uptake glycine. Methods The labeled glycine in L 929 cells was measured by scintillation technique. After L 929 cells were incubated with labeled glycine and steroid and/or other chemical reagents, the effects of steroids and their mechanism were determined. Results Corticosterone, aldosterone, estradiol, dexamethone and hydrocortisone inhibited glycine uptake by L 929 cells to various extents. There was no substantial difference between the effects of bovine serum album conjugated corticosterone and corticosterone 21 sulfate on glycine uptake. The inhibitor of G protein, GDP ? S, could partially block the effects of corticosterone and aldosterone. The inhibitor of phospholipase C, neomycine, did not inhibit the effect of corticosterone. The inhibitor of protein kinase C, Chelerythrine, partially blocked the effect of corticosterone. The activator of protein kinase C (phorbol 12 myristate, 13, acetate) seemed to imitate the effect of corticosterone. The activator of cAMP, Forskolin, and the inhibitor of protein kinase A, H 89 , blocked the effect of corticosterone. Conclusion The rapid inhibitory effects of steroids on glycine uptake in L 929 cells are nongenomic, and their signal transduction is through the pathway of G protein protein kinase C.

AIM To investigate the nongenomic effects of steroids on glycine uptake in L 929 cells. METHODS L 929 cells were incubated with labeled glycine, steroids, and/or other reagents. With liquid scintillation technique, the labeled glycine in L 929 was measured. RESULTS Steroids could rapidly inhibit the glycine uptake. Action intensity of steroids was different. Effects of CORT and ALD were dose-dependent. There was no difference in effects between corticosterone 21-sulfate and B-BSA. Inhibitor for cytoplasm receptor of glucocorticoids could partially block the effect of CORT. Extracelullar Ca 2+ could influence the effect of CORT. CONCLUSION Effects of steroids on glycine uptake in L 929 cells are nongenomic. Steroids may take effect through membrane receptors. The receptors of CORT in membrane are similar to those of glucocorticoids in cytoplasm.

Survivin has been identified recently as a unique member of the inhibitor of apoptosis protein family. It is expressed during fetal development and in all common human cancers, but not in normal tissues. It can inhibit apoptosis induced by a variety of factors and play an important role during angiogenesis. Being a universal tumor-specific antiapoptosis inhibitor, survivin may serve as a potential target for gene-protein-based therapy of tumor.

Objective To investigate effects of rosiglitazone on glucose transport-4(GLUT4) protein translocation in skeletal muscle of insulin resistance rats. Methods 38 male sprague dawley rats were randomized to received high fat or standard chow diet for 8 weeks, feeding high-fat diet to induce insulin resistance in skeletal muscle of rats. 25 high-fat-fed rats were randomly treated with rosiglitazone(1mg/kg), standard chow diet, or placebo(high-fat diet) for 4 weeks. Plasma concentrations of glucose and insulin, weight and viscero-fat in all rats were measured. The GLUT4 level in the cell membrane of isolated rats skeletal muscle was detected by western blotting analysis. Results Insulin-stimulated translocation of GLUT4 to the plasma membrane in skeletal muscles of high fat-fed rats was significantly lower than that of standard chow-fed rats(52 72%,P

OBJECTIVE:To promote the work and socialization of out-patient pharmacy in special hospital.METHODS:Using the methods of order of sum of money and frequency analysis,consumption structure and sale sum of drugs and frequency of drugs used in this hospital in 1999～2000 were analyzed.RESULTS:Anti-infectives,drugs for special clinic and hormonal preparations were the commonly-used drugs,which indicated that most patients suffered from external genital organ infections The consumption of hormonal preparations increased obviously,which implied that the popularization of hormone replacement therapy and gradual maturity of subsidiary fertility technicology in this hospital.CONCLUSION:The analysis shows the characteristics of drug-use in obstetrics and gynecology hospital.

Objective To evaluate the efficacy of arthroscopic one-stage reconstruction of anterior and posterior cruciate ligaments with LARS artificial ligaments. Methods From October 2009 to December 2011, 8 patients with combined injury to anterior and posterior cruciate ligaments received arthroscopic one-stage reconstruction with LARS artificial ligaments. They were 7 men and one woman, aged from 26 to 52 years ( average, 35. 3 years ) . The time from injury to operation ranged from 17 to 21 days, averaging 19 days. Pre-operative and intraoperative inspections showed complete tear of both anterior and posterior cruciate ligaments. Inter national Knee Documentation Committee ( IKDC ) scoring was used at 6 months postoperation to grade the knee movement of the patients; Lysholm scoring was used at final follow-ups to evaluate the knee function. Results The operating time for this series ranged from 85 to 102 minutes ( average, 93 minutes ) . All the patients were followed up for 6 to 24 months ( average, 12 months ) . Wound infection at upper tibia occurred in one patient, which responded to debridement and screwing 4 weeks after operation. All the patients got rid of instability of the knee joint, and gained normal knee extension and fine functional recovery. At final follow-ups, the range of motion of the knee ranged from 100° to 125° ( average, 108. 4°) . Follow-ups revealed no breakage or loosening of the ligaments. By the IKDC scoring, of the 8 patients who had been graded all as D before opera-tion, one was improved into A and 7 into B. The Lysholm scores at final follow-ups averaged 86. 1+4. 8, sig-nificantly higher than the postoperative value ( 18. 7+3. 1 ) ( P < 0. 05 ) . Conclusion Arthroscopic one-stage reconstruction of anterior and posterior cruciate ligaments with LARS artificial ligaments can restore the stability of knee joint and obtain satisfactory short-term results.

Objective:To investigate the clinical efficacy of psychotherapy combined with auricular plaster therapy in the auxiliary treatment of insomnia caused by liver fire disturbance in a sub-healthy population.Methods:Eighty sub-healthy patients with insomnia caused by liver fire disturbance who received treatment in Wenzhou Hospital of Traditional Chinese Medicine from June 1, 2017 to December 31, 2018 were included in this study. These patients were randomly assigned to receive cognitive behavioral therapy (control group, n = 40) or cognitive behavioral therapy combined with auricular plaster therapy (observation group, n = 40) for 4 weeks. Polysomnography was used to evaluate the sleep condition before and after treatment. The Pittsburgh Sleep Quality Index was used to evaluate the severity of insomnia. The World Health Organization Quality of Life Scale Brief Version (WHOQOL-BREF) was used to evaluate the quality of life of patients. The therapeutic effect was compared between the two groups. Results:After treatment, total sleep time [(421.25 ± 11.58) min vs. (456.25 ± 11.23) min], sleep efficiency [(70.32 ± 4.44)% vs. (76.58 ± 5.89)%], non-rapid eye movement stage 2 (N2) sleep [(43.25 ± 4.44)% vs.(45.62 ± 4.36)%], N3 sleep [(14.25 ± 4.52)% vs. (18.52 ± 5.55)%], rapid eye movement (R) sleep [(21.98 ± 5.21)% vs. (24.66 ± 4.87)%] in the control group were significantly shorter or lower than those in the observation group ( t = 13.723, 5.367, 9.035, 2.408, 3.772, 2.376, all P < 0.05). After treatment, sleep quality [(1.85 ± 0.54) points vs. (1.10 ± 0.42) points], sleep-onset latency [(1.52 ± 0.51) min vs. (1.08 ± 0.35) min], sleep duration [(1.30 ± 0.41) min vs. (0.75 ± 0.41) min], sleep efficiency [(1.53 ± 0.44) points vs. (1.02 ± 0.33) points], sleep disturbance [(1.20 ± 0.41) points vs. (0.78 ± 0.31) points], hypnotics [ (0.11 ± 0.02) points vs. (0.04 ± 0.01) points], daytime dysfunction [(1.65 ± 0.47) points vs. (1.03 ± 0.28) points] and total scores [(9.11 ± 1.33) points vs. (5.80 ± 1.01) points] in the control group were significantly higher than those in the observation group ( t = 6.933, 4.499, 5.999, 5.864, 5.167, 19.789, 7.167, 12 535, P < 0.05). After treatment, the scores in physical health [(13.85 ± 2.22) points vs. (17.86 ± 2.01) points], psychological domain [(15.52 ± 1.89) points vs. (16.35 ± 1.33) points], social relationships [(16.01 ± 1.58) points vs. (19.52 ± 1.85) points] and environment domain [(17.52 ± 1.90) points vs. (20.89 ± 2.22) points] in the control group were significantly lower than those in the observation group ( t = 8.468, 4.499, 9.124 and 7.294, all P < 0.05). After treatment, total effective rate in the observation group was significantly higher than that in the control group [95.0% (38/40) vs. 72.5% (29/40), χ 2 = 7.440, P < 0.05]. Conclusion:Psychotherapy combined with auricular plaster therapy can significantly increase the sleep duration and sleep efficiency and improve the quality of life of sub-healthy patients with insomnia caused by liver fire disturbance, which is worthy of clinical promotion.

Objective To observe the effects of exercise training on the expression of glycogen synthase ki-nase-3 (GSK-3) in the skeletal muscles of rats with insulin resistance induced by a high-fat and high sucrose diet. Methods Thirty Wistar rats were randomly divided into three groups: a control group, a model group and an exercise group. Rats in the model and exercise groups were fed with a high-fat and sucrose diet (20% sucrose, 10% lard, 2.5% cholesterol, 1% cholic acid) for 4 weeks, and then the rats in the exercise group were trained by swimming 5 d every week for 6 weeks. After treatment, the concentration of GSK-3 protein in their muscles was detected by Western blot analysis, while their weights, serum triglyceride and total cholesterol, and their concentrations of fasting plasma glucose and fasting plasma insulin were measured periodically and their insulin sensitivity index was calculated. Results Compared with the controls, the expression of GSK-3 in the skeletal muscles of the rats in the model group increased by 70. 20% (P