OBJECTIVE:To establish the quality criteria for Xiaoyan tablets. METHODS: Herba Taraxaci and Herba Violae in the formula were identified qualitatively by TLC and the content of Baicalin was determined by HPLC on Zorbax SB-C18(250 mm?4.6 mm,5 ?m) column with mobile phase consisted of acetonitrile-0.4%H3PO4(24∶76) at a flow rate of 1.0 mL?min-1. The detection wavelength was set at 280 nm and the column temperature was set at room temperature. RESULTS: Herba Taraxaci and Herba Violae could be detected by TLC. The linear range of Baicalin was 0.08～2.49 ?g(r=0.999 9). The average recovery rate was 100.24%(RSD=1.52%,n=6).CONCLUSION: The establish criteria are suitable for the quality control of Xiaoyan tablets.

China ; Hepatectomy ; methods ; trends ; Humans

Objective: To establish human multidrug-resistant hepatocellular carcinoma cell line(QGY7703/DDP) to analyze its different resistance and sensitivity. Methods: The human hepatocellular carcinoma cell line QGY7703 was continuously grown in the presence of cisplatin（DDP) for up to 6 months. Cell proliferation and cytotoxicity were serially monitored. Results: The chemosensitivities to 5-FU，DDP，adramycin (ADM) and mitomycin(MMC) were different between QGY7703 and QGY7703/DDP. The exhibitions were 1.533 fold to 5－FU，2.181 fold to DDP，7.08 fold to ADM, 9.461 fold to MMC in QGY7703/DDP. Conclusion: The drug resistance in cancer cell lines can be established by exposing to antitumor drugs in cell culture system.

Objective:To put forward some suggestions by comparing the current situation in both private and public hospitals. Methods:Comparing the basic information, scale, human resource and medical service management situation between the private and public hospitals by analyzing the 2014 Beijing health authorities report. Results:There are 355 private hospitals, 76. 1% of them are not rated and 59. 7% are not appointed for the medical insur-ance, and 82. 8% need to rent the land. Although the number of private hospitals is more than that of the public ones, their actual beds and health resources respectively account for only 20. 2% and 16. 4%. Besides, their in-come, assets and services also account for only about 10%. Conclusions:There is a big gap between the private and public hospitals in terms of scale, human resources and service ability. The Government should implement the policy about the ratings, medical insurance and the use of land which is given to support the private hospitals, and speed up the diversified medical model.

Objective To investigate the clinical curative effect of combined treatment of mometasone furoate nasal spray, loratadine, health education on children with allergic rhinitis and effects on IL-10 and IL-17. Methods 50 children with allergic rhinitis in our hospital from January 2015 to December 2016 were selected,and according to the different treatment methods divided into observation group and control group,25 cases in each groups.The control group were treated with loratadine, observation group with mometasone furoate nasal spray of loratadine + + health education treatment; we observed the two groups before and after treatment in children with nasal symptoms score, IL-10 and IL-17 and the incidence of adverse reactions, and the relevant data for comparative analysis. Results Mometasone furoate nasal spray of loratadine + + Health Education (observation group) the effect of treatment of children with allergic nasal is better than loratadine (control group) treatment, symptoms to improve the situation, IL-10 and IL-17 are better than the control group, the difference was statistically significant (P<0.05); two groups children with adverse reaction rate comparison, the difference was not statistically significant. Conclusion Children with allergic rhinitis selection of mometasone furoate nasal spray + loratadine + health education treatment effect significantly, can effectively improve the symptoms, IL-10 and IL-17, and will not increase the incidence of adverse reactions, it is worthy of clinical application.

0.97).The method of Real-time PCR and molecular beacon detection for bifodobacteria has many advantages,such as being sensitive,specific,simple and fast,and this method can be used in situ detection of bifidobacteria quantitatively.

Animals ; Carcinoma, Hepatocellular ; genetics ; virology ; Hepatitis B, Chronic ; complications ; Hepatitis C, Chronic ; complications ; Humans ; Liver Neoplasms ; genetics ; virology ; Neoplasm Recurrence, Local ; genetics ; Point Mutation ; Telomerase ; metabolism

OBJECTIVE:To establish a HPLC method for content determination of paeoniflorin in refined coronary tablets.METHODS:HPLC was performed on Kromasil C18 column at room temperature,the mobile phase consisted of acetonitrile-0.2%phosphoric acid solution(13∶87),the detection wavelength was 230nm with flow rate 1ml/min and sample size 10?l.RESULTS:Paeoniflorin was linear with the peak area in the range of 0.317?g～1.587?g(r=0.9 999),the average recovery was 100.1%(RSD=1.46%).CONCLUSION:The established method is simple,reliable,reproducible and can be used for the quality control of refined coronary tablets.

Absorption ; Ammonia ; chemistry ; Environmental Monitoring ; methods ; Materials Testing ; Silica Gel ; chemistry

Early diagnosis of hepatocellular carcinoma(HCC)is still a great challenge in clinical practice.Tumor markers such as alpha-fetoprotein(AFP),liver enzymes,cytokines,and some special glycoproteins,though helpful,are not sensitive and specific enough for early diagnosis of HCC.The establishment of several interesting predictive diagnostic models on liver fi- brosis/cirrhosis suggests that mathematic predictive model,which is developed based on large sample size and follow-up study, might be of higher sensitivity,specificity and feasibility in clinical application.Here we suggest that more attention should be paid to this kind of multi-parameter predictive diagnostic models clinically,so as to improve the early diagnosis of HCC in a more economical and feasible way.