The effects of various digestive enzymes, fresh biles, and bile acids on the hatching and activation of the eggs of Taenia saginata, T. pisiformis, T. solium, Hymenolepis nana, and Moniezia expansa have been investigated. On the basis of the results of the present study author attempted to elucidate the specificity of host-parasite relationships in the tapeworm infections. The results were summarized as follows: In case of the ova of T. saginata, pretreatment with artificial gastric juice before contact of intestinal juice is required to bring the disintegration of the embryophore and activation of the embryo. However the ova of T. solium and T. pisiformis may be disintegrated and activated directly into the artificial intestinal juice without the above pretreatment. Among the digestive enzymes applied in this experiment, trypsin was found most effective on the hatching. No hatching of the ova of H. nana and M. expansa was observed in the above artificial hatching-activating solutions. The fresh biles and bile salts were more effective on the hatching and activation of Taeniid ova than sodium taurocholate and sodium deoxycholate. It is turned out that the digestive enzymes or bile acids in the intermediate hosts may, not apparently be essential factor of determining the specificity of host-parasite relationships.
parasitology-helminth-cestoda ; Taenia saginata ; Taenia pisiformis ; Taenia solium ; Hymenolepis nana ; Moniezia expansa ; egg hatching ; host-parasite relationship ; trypsin ; artificial intestinal juice

We studied the serological reaction between various antigenic components from Cysticercus cellulosae and IgG antibodies in sera of cysticercosis, sparganosis, hydatidosis patients and normal humans by ELISA and EITB. In serological tests by ELISA, we recognized cross reaction of Cysticercus antigenic components with IgG antibodies in heterologous sera such as sparganosis and hydatidosis patients or normal humans. The crude antigenic components of Cysticercus showed lower ELISA sensitivity in homologous sera from cysticercosis patients than heterologous sera from hydatidosis patients. A total of 31 polypeptide bands with 260 KDa-22 KDa molecular weights were detected by SDS-PAGE, and 11 of them showed strong intensity. Total 22 components of them were recognized by IgG antibodies in cysticercosis patients sera. However, 12 of them were recognized also by normal human sera, 11 were by sparganosis sera, and 21 were by hydatidosis patients sera. The crude antigenic components of 104 KDa, 82 KDa, 72 KDa, 59 KDa and 34 KDa molecular weights were nonspecific ones, which cross-reacted with sera of either cysticercosis, sparganosis, hydatidosis patients or normal humans.
parasitology-helminth-cestoda ; Spirometra erinacei ; Taenia solium ; cysticercus ; sparganum ; immunology ; antigen

An electron microscopic study was performed to know the basic tegumental structure of Cysticercus cellulosae. The scolex and bladder portions of cysticerci (human and porcine strains) were prepared for transmission and scanning electron microscopy by conventional procedures. In general, the tegument of C. cellulosae showed the basic ultrastructure of cestode tegument on electron micrographs. The teguments of both scolex and bladder portions consisted of such components i.e., an outer vesicular layer with numerous microtriches and inner fibrous layer. Below the fibrous layer, there were layers of muscle bundles and tegumental cells. The microtriches which covered the surface of cysticercus revealed two distinctly different shapes. The characteristic bladder-like, elongated pyramid shaped 'tetrahedral form' was observed on the surface of the scolex portion, whereas the elongated cylindrical 'filamentous form' was distributed on the surface of bladder portion. In spite of the difference of isolated host and location, the cysticerci showed the same result. But dimensional variations of the tegument according to topography of the worm were observed. The possibility of application in making differential diagnosis from other larval cestodes and possible functions of this larval tegument were discussed.
parasitology-helminth-cestoda ; Taenia solium ; cysticercus ; electronmicroscopy

From 1960 through 1965, 39 cases with epileptic seizure caused by the Cystricercus cellulosae were experienced at Kaejong Mental Hospital, Okku-Gun, Chollapukdo province. The present study was carried on at the area of Chollapukdo province where Cysticercus cellulosae cases were frequently reported. Thrity-nine Cystricercus cellulosae cases(35 male and 4 female) with epileptic seizure visited Kaejong Mental Hospital . Okku-Gun, Chollapukdo during the years of 1960-1965 from various Gun(county): Okku-Gun 13, Kimje-Gun 7, and Soonchang-Gun 6. The high incidence was found at the age group of 20-49 years and the distribution of 214 subcutaneous nodules were as follows: Trunk 56.6 percent, upper extremities 26.6 percent, head and neck 9.3 percent, lower extremities 7.5 percent. According to the address of above 39 cases, taenia infestation of the inhabitants in the area was examined by questionaire form. Plain rural area (Maryong-Ri, Okku-Gun); Among 803 persons interviewed, 4.0 percent or 32 complained the output of Taenia segments. The male group showed higher incidence (6.4 percent) than the female(1.5 percent) . The highest incidence was observed at the age group of 30-39 years. Most of the inhabitants of the area had the eating habit of raw beef and pork. Mountainous rural area(Bok-Heung-Myun, Soonchang-Gun); Among 542 persons interviewed, 33 or 6.1 percent complained Taenia infection. The higher incidence was observed in the male group(8.7 percent) than in the female group(3.1 percent) , and also at the age group of 20-49 years. They had eating habit of raw beef and pork. The species of adult worm were identified by morphological examination. Totally 36 adult worms(Taenia saginata 21, T. solium 15) were obtained from 26 cases, and it was experienced that 5 T. solium were eliminated from one person.
parasitology-helminth-cestoda ; Taenia solium ; cysticercus ; epidemiology

In order to obtain some informations on the nature and relative activity of the phosphatases present in various helminths, biochemical studies have been made in thirteen kinds of worm parasites including the adults and larvae (Fasciola hepatica, Eurytrema pancreaticum, Paramphistomum sp., Taenia solium, Taenia pisiformis, Dipylidium caninum, Diphyllobothrium mansoni, Cysticercus cellulosae, Cysticercus fasciolaris and Sparganum). A comparison based on the analysis of pH-activity curves was made among these helminths. The worm materials were mostly obtained alive from an abattoir and removed from the organs or tissues of the animal hosts naturally infected. Sparganum and Cysticercus cellulosae, however, are collected from the subcutaneous tissue of the patients by surgical removal. The worms thoroughly washed were weighed and transferred with 0.1 M Tris buffer to a chilled glass grinder (Capacity; 15 ml) and homogenized in the cold. The homogenate was centrifuged at 5000 RPM for 30 minutes. The supernatant was pipetted off for determination of the phosphatase activity. Incubation mixtures consisted of 1 ml substrate, 1 ml buffer and 0.5ml extract. The buffers used were Tris (Hydroxymethyl) aminomethane and citric acid monohydrate and the substrate was paranitrophenyl phosphate (1 gm/25 ml). These mixtures were incubated at the temperature of 37 C for 30 minutes in water bath. The absorbance or transferance of mixture was determined colorimetrically by "Spectronic 20 "spectrophotometer at 410 nm against a distilled water blank. The amount of phenol liberated was then calculated from a standard curve using phenol solutions. Controls consisted of unincubated mixtures. The results were deducted from this experiment. The phosphatase activity occurred over all parasitic helminths used in this experiment. In trematodes, pH-activity curves have demonstrated two peaks of phosphatase activity in Fasciola hepatica and Paramphistomum species. However the acid phosphatase activity was predominantly found and the alkaline phosphatase activity was found distinctly to be low in all three species. In Eurytrema pancreaticum, the pH-activity curves displayed two peaks in acid phosphatase activity, one at pH 5.0 and the other pH 9.0. In cestodes, both alkaline and acid phosphatase activity displayed the pH optima 5.0 and 9.0 to 10.0 in the adult tapeworms. However, major activity in the adults is due to the alkaline phosphtases. In contrast to the adults, Cysticercus and sparganum showed the higher activity in acid phosphatases which predominates in the larvae. In all cases of nematodes, the pH optimum for acid phosphatase was 4.0 to 6.0. A preponderance of acid phosphatase activity was shown in the extract of intestine of Ascaris lumbricoides. The aspect that phosphatases are correlated with phosphorylated passage of substances through the cuticle of helminths and may also be involved in carbohydrate metabolism is discussed.
parasitology-helminth-trematoda-cestoda ; Fasciola hepatica ; Eurytrema pancreaticum ; Paramphistomum sp. ; Taenia solium ; Taenia pisiformis ; Dipylidium caninum ; Diphyllobothrium mansoni ; Cysticercus cellulosae ; Cysticercus fasciolaris ; sparganum ; alkaline phosphatase ; acid phosphatase ; biochemistry

Total of 2,643 Korean soldiers were examined of their stool for parasitic infections by both cellophane thick smear and formalin-ether concentration techniques from August 1983 to December 1985. Out of them, 73.6% were free from any parasite, 22.6% were ova positive and 4.0% cyst positive. The ova positive rates by species were Ascaris lumbricoides 2.0%, Trichuris trichiura 13.0%, hookworm and Trichostrongylus orientalis 0.08% respectively, Clonorchis sinensis 7.6%, Metagonimus yokogawai 1.1%, Paragonimus westermani 0.08%, Echinostoma hortense 0.04%, Fibricola seoulensis 0.9%, Taenia sp. 0.3%, Hymenolepis nana 0.2% and H. diminuta 0.04%. Most of them (87.3%) were positive by a species, 11.9% by two and 0.8% by 3 species. The cyst positive rates were Entamoeba histolytica 0.6%, E. coli 1.4%, Endolimax nana 1.7%, Iodoameba butschlii 0.04% and Giardia lambla 0.9%. Among the cyst positives, 89.5% were positive by a species, 7.6% by two and 0.5% by 3 species. The intestinal parasite infections among the Korean soldiers decreased distinctly compared with previous data.
parasitology-helminth-nematoda ; trematoda ; cestoda ; protozoa ; epidemiology ; Clonorchis sinensis ; Trichuris trichiura ; Metagonimus yokogawai ; hookworm ; Ascaris lumbricoides ; Diphyllobothrium latum ; Hymenolepis nana ; Entamoeba histolytica ; Giardia lamblia ; Entamoeba coli ; Endolimax nana ; Trichostrongylus orientalis ; Paragonimus westermani ; Echinostoma hortense ; Fibricola seoulensis ; Taenia sp. ; Hymenolepis diminuta

An investigation of the helminth parasites of house rat R. norvegicus Erxl. in Seoul has been carried on four years. A total of 325 rat was examined. Ten species of helminths were considered, of which 4 species were recored for the first time from Korea. The adult worm of Capillaria hepatica, the most common parasite of house rats in Seoul, was observed experimentally, and provided a more complete description of this worm.
parasitology ; epidemiology ; helminth ; trematoda ; nematoda ; cestoda ; Echinostoma hortense ; Fibricola seoulensis ; Taenia taeniaformis, Hymenolepis nana ; Hymenolepis diminuta ; Heterakis spumosa ; Syphacia obvelata ; Nippostrongylus muris ; Trichosomoides crassicauda ; Capillaria hepatica

Ten cases infected with Taenia saginata were treated with Bithionol, 2, 2' thiobis (4,6-dichlorophenol) which is well known as an excellent anthelminthhic against paragonimiasis and their follow-up studies were carried on within 3 to 12 months after treatment. Bithionol, 50 mg dose per kg of body weight was divided into two equal dose and administered orally in 30 minutes intervals. Three hours later, this was followed with a sodium sulfate or magnesium sulfate purge. The strobilas without scolices were found in the stools after the medication and the follow-up studies have shown the complete deworming in all cases. It is well tolerated, toxic effects being uncommon and when present, limited to mild gastro-intestinal disturbances.
parasitology ; helminth ; cestoda ; Taenia saginata ; chemotherapy ; bithionol

By affinity chromatography using a monoclonal antibody as ligand, Kim et al. (1986) purified a protein fraction in cystic fluid of Taenia solium metacestodes (CF). In this study, the biochemical properties of the purified protein were characterized. Discontinuous-polyacrylamide gel electrophoresis (disc-PAGE) of the protein at 4.5~10% separating gel concentration showed its molecular weight (MW) to be 150 kilodalton(kDa) in non-denatured state, while denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that it was composed of 3 different subunits with respective MW of 15, 10 and 7 kDa. Subunit of 7 kDa was shown to be linked to other subunits by disulfide bonds. Isoelectric point of the protein was pH 6.8. The protein was relatively heat-stable for immunologic analysis. These properties indicated that the protein, comprising about 70% of total content in CF, had similar biochemical characters with antigen B of Oriol et al.(1971) in hydatid cyst fluid (HF)
parasitology-helminth-cestoda ; Taenia solium ; metacestode ; protein ; antigen ; cysticercus ; biochemistry

An acephalic budding Cysticercus of 1.2 cm long was removed surgically at the abdominal wall of a Korean man. The worm revealed abnormal buds on the bladder wall and absence of suckers and hooklets in the scolex body. The buds were of two histologic types; branching bud covered with normal tegumentum and with subtegumental cells of normal density, and buds of proliferated subtegumental cells with lacunae formation. On the bases of the morphologic features, it was identified as a racemose cysticercus. This case confirms that its extracranial location is possible.
parasitology-helminth-cestoda ; Taenia solium ; cysticercus ; case report