[ABSTRACT]AIM:Toexploretheeffectsofchrysinoninsulinresistance(IRe)inamousemodel.METHODS:Male C57 mice were randomly divided into control group , IRe group, low-dose chrysin group ( IRe+chrysin-low) and high-dose chrysin group (IRe+chrysin-high).After 24 weeks, the body weight, liver index and fat mass in all mice were detected.The blood glucose , insulin level and HOMA-IR were measured to determine the changes of the insulin resistance in the animals.The oxidative stress (SOD, GSH-Px and MDA) was also measured.The mRNA expression of insulin sig-naling pathway molecules (IR, IRS1, IRS2, Glut2 and Glut4) and inflammatory factors (TNF-α, IL-1β, IL-6 and NF-κB) was analyzed by real-time PCR.The protein levels of IRS1 and p65, and their phosphorylation were detected by West-ern blot.RESULTS:After 24-week intervention , the indicators in IRe group were higher than those in control group , in-cluding body fat deposition, serum glucose, serum insulin, HOMA-IR and liver oxidative stress (P<0.01), indicating that the model of insulin resistance was successfully established .Low dose and high dose of chrysin decreased the body weight, serum glucose, serum insulin and HOMA-IR in the IRe mice (P<0.05).The liver oxidative stress was also re-duced in both groups (P<0.05).However, no statistical difference of the indexes between IRe +chrysin-low group and IRe+chrysin-high group was observed.Chrysin upregulated the mRNA expression of IR , IRS1, IRS2, Glut2 and Glut4 (P<0.05), and down-regulated the mRNA expression of various inflammatory factors .The inhibitory effect of chrysin on the mRNA expression of NF-κB was observed (P<0.05), especially in high dose group (P<0.05).It was confirmed that the effect of chrysin on liver IRe was related with the increase in the p-IRS1 levels and decrease in the p-p65 levels by Western blot .CONCLUSION:Chrysin inhibits obesity , hyperglycemia and hyperinsulinemia , and relieves insulin resist-ance and oxidative stress , which might be closely related to the regulation of insulin signaling pathway and the inhibition of inflammatory factor expression .

Objective To discuss the influence of clean-up frequency of the operative cavity on the curative effect after endoscopic sinus surgery.Methods 368 patients with sinusitis or nasal polyps were randomly divided into observation group and control group,184 patients in each group.Control group was treated with operative cavity cleaning in moderate frequency,while observation group in intensive frequency.Results The obvious effective rate of observation group was 80.98％,which was significantly higher than that of control group(69.02％) (x2 =12.468,P ＜0.05).The ineffective rate of observation group was 2.17％,which was significantly lower than that of control group (9.78％) (x2 =15.717,P ＜ 0.05).The recurrence rate of observation group was 11.96％,which was significantly lower than that of control group (26.09％) (x2 =9.361,P ＜ 0.05).Conclusion The clean-up frequency of the operative cavity after endoscopic sinus surgery can influence curative effect,moderate clean-up frequency can significantly improve the treatment effects and deserved promotion.

Objective:Toinvestigate the influence of MMP7 antisense oligodeoxynucleotide(ASODN)on adhesion and invasion ofhuman lung adenocarcinoma cell line A549.Methods:Phosphorothioate MMP7 ASODN was transfected to A549 cells mediated by liposome. The expression of MMP7 was examined by RT-PCR.The adhesive and invasive ability were examined by plate adhesion model and Boyden Chamber transwell assay.Results:After MMP7 ASODN was transfected,the relative optical density(ROD)of electrophoresis strip was decreased obviously(P

Objective To discuss the application of core needle aspiration biopsy to diagnosis of breast csncer. Methods 64 cases with breast cancer tumor were checked byCNB and compared with surgical pathology results.Then the method of CNB operation technology was analyzed. Results The diagnostic sensitivity was 96.0% and specificity was 100%. There was no complication during biopsy. The false negative raty was 4.0%. Conclusion The application of core needle aspiration biopsy was simple, safe and reliable. And it could improve the diagnosis of breast cancer.

Transcranial doppler (TCD) was used to measure the flow velocities of middle cerebrial artery (MCA) in 4 patients of clinical brain death. The reaults showed that the changes were similar in all the patients studied; 1) the dierotic notch (DN) was deeper in the spectrum of flow velocities of MCA, end diastolic flow rate (VD) significantly decreased, and the pulastility index (PI) increased ; 2) VD decreased progressively; 3) all patients showed characteristic to-and-fro TCD pattren with antegrade flow in systole and retrograde flow in diastole; 4) the pattern totally disappeared when the patients were claimed dead. It is found that TCD is valuable for the determination of brain death.

Objective To analyze the pathophysiological variation in patients with penetrating cardiac trauma (PCT) and discuss the classified treatment strategies.Methods A retrospective analysis was conducted on pathogenetic condition,medical interventions and other clinical information of 133 PCT patients.The patients were divided into four groups in accordance with their symptom classification on arrival at emergency department,including sub-clinic type,cardiac tamponade type,hemorrhagic shock type and agonal type.Meanwhile,the patients were classified into three groups according to their pathogenesis phase,including sub-clinical phase,clinical phase and agonal phase.Development characteristic of patients' condition in each group was investigated in each time point by survival analysis.Impacts of medical interventions on prognosis of those patients were analyzed,such as pericardial space exploration,emergency room thoracotomy (ERT),operating room thoracotomy (ORT).Results Some patients at sub-clinical phase were aggravated into clinical phase or agonal phase,as well as some patients at clinical phase were aggravated into agonal phase during in-hospital treatment.There were significance differences of posttraumatic suvival course among the four groups,namely sub-clinical type,cardiac tamponade type,hemorrhagic shock type and agonal type (P ＜ 0.01).The differences of posttraumatic survival course were also significant among the three groups,namely sub-clinical phase,clinical phase and agonal phase (P ＜ 0.01).Conclusion Clinical symptom classification (or pathogenesis phase) of PCT may not be always unchangeable,thus it is recommended that PCT patients should be treated based on their clinical symptom classification or patbogenesis phase at consultation.

Useful pharmacodynamic changes occur when some Chinese medicine are used together with some Western medicine, namely enhanced curative effect, lowered adverse reactions, reduced dosages, shortened treatment courses, enlarged indications scope, improved compliance of treatment and rational medication, which could be explored to provide scientific bases for further improving diagnosis and treatment levels and rational use of drugs.
Drugs, Chinese Herbal ; metabolism ; Humans ; Medicine, East Asian Traditional ; Pharmaceutical Preparations ; metabolism ; Pharmacokinetics ; Phytotherapy

Noninvasive Ventilation ; Positive-Pressure Respiration ; Pulmonary Heart Disease ; complications ; Silicosis ; physiopathology ; Ventricular Function, Right

No abstract available.
Organ Transplantation* ; Transplants*

Enterovirus 71 (EV71) is the main causative agent of hand, foot, and mouth disease (HFMD). This article presented the inhibitory activity of pentapeptides on the EV71 infection in rhabdomyosarcoma (RD) and suckling mice. The EV71 VP1 capsid protein expression levels and mRNA levels were analyzed by Western blotting and real-time PCR. The antiviral activity of pentapeptides in vivo was evaluated by weight changes and EV71 VP1 protein expression levels in intestines of suckling mice. Results revealed that the pentapeptide P010157 was able to inhibit EV71 replication in RD cells. After being incubated with the P010157 at a concentration of 100 microg x mL(-1) for 48 h, the level of EV71 vp1 mRNA in RD cells decreased by (92.0 +/- 6.3)%. The estimated EC50 was 2.2 microg x mL(-1). P010157 was able to inhibit EV 71-induced cytopathic effect (CPE) in RD cells. The cytotoxic activity of the compound was evaluated against RD cells by MTS assay. The results showed that P010157 had no obvious toxicity. In addition, the treated mice with P010157 did not exhibit weight loss, as was observed in untreated mice. EV71 replication reduced significantly as revealed by Western blotting. These findings suggest that P010157 could prevent EV71 proliferation in vitro and in vivo. P010157 is a novel compound for antiviral therapies against EV71, which merited further investigation.
Animals ; Antiviral Agents ; pharmacology ; Capsid Proteins ; genetics ; metabolism ; Enterovirus A, Human ; physiology ; Enterovirus Infections ; metabolism ; Humans ; Mice ; Oligopeptides ; pharmacology ; RNA, Messenger ; metabolism ; Rhabdomyosarcoma ; metabolism ; pathology ; virology ; Tumor Cells, Cultured ; Virus Replication ; drug effects