1.Exploration on employment-oriented practice mode of combing learning with working for pharmaceutical engineering specialty
Bing GU ; Chunqing CHENG ; Zhiyong LIN ; Pengwu ZHENG
Chinese Journal of Medical Education Research 2011;10(4):405-407
Through analyzing the employment situation of pharmaceutical engineering specialty,a variety of employment-oriented practice mode of combining learning with working has been explored,including internships,school-enterprise cooperation,personal contact przctice,combined with the dual selections fair,co-culture with other universities,and attending postgraduate exanl with a research proiect model.As a result,the implementation of these models led to improve students'professional operating skills and eventually encourage graduates to start a career smoothly
2.Duplication of animal models for spinal cord injury and its application in experimental therapeutics
Bing GU ; Zheng ZHANG ; Huanan LI ; Wei MENG ; Riyue YU
Chinese Pharmacological Bulletin 2009;25(12):1559-1562
The mechanism of spinal cord injury and repair therapy after nerve injury is currently a hotspot of neuroscience research.Duplicating animal models plays a key role in experimental therapeutics of spinal cord injury.This review systematically describes the progress in animal models for spinal cord injury including contusion, compression, transection, ischemic,distraction and chemical-mediated injury,which have been established at home and abroad.Based upon the aforementioned models,some applications in experimental therapeutics are simultaneously enumerated.All these information provides scientific guidance for the experimental novel drugs′screening.
3.The effect of fat emulsion intravenous infusion on serum free fatty acids in rats
Wei ZHENG ; Chuanbo ZANG ; Bing MA ; Rong LI ; Zhuoyu GU
Chinese Journal of General Surgery 2001;0(08):-
Objective To study the effect of fat emulsion intravenous infusion on serum free fatty acids(FFAs) in rats.Methods 24 male Wistar rats were divided randomly into 3 groups,8 rats in each group.(1)Control group(NS),the rats were infused with normal saline intravenously and regular chow;(2)Group LCT,infused with 10% intralipid fat emulsion intravenously;(3)Group MCT/LCT,infused with 10% lipofundin fat emulsion. Group LCT and group MCT/LCT were continuously received equal calorie,nitrogen and volemin in 'All-in-One'solutions. Serum samples were drawn on the 8th day after PN for fatty acid determination. Results The FFAs in Group LCT and group MCT/LCT were remarkably higher than that in control group, but no difference between Group LCT and group MCT/LCT. Conclusions Fat emulsion intravenous infusion can increase the serum free fatty acids considerately.
4.Evaluation of the correlation between cerebral venous sinus and idiopathic intracranial hypertension with cerebrovascular intervention technique
Zhenchan LU ; Bing ZHANG ; Shaojun ZHENG ; Binxian GU
Journal of Interventional Radiology 2017;26(2):105-108
Objective To discuss the application of cerebrovascular intervention technique in evaluating the correlation between cerebral venous sinus and idiopathic intracranial hypertension (ⅡH).Methods The clinical data,including complete related clinical signs and symptoms,laboratory results,magnetic resonance venography (MRV) findings,etc.,of 13 ⅡH patients were retrospectively analyzed.Under local anesthesia whole brain DSA was performed,and superselective catheterization of venous sinus was accomplished to perform sectional pressure measurement of sagittal sinus,transverse sinus,sigmoid sinus and jugular vein.Results Cerebral DSA examination revealed that venous sinus stenosis or occlusion was seen in 10 patients (76.9%),including transverse sinus (n=6),sigmoid sinus (n=3),and transverse sinus together with sigmoid sinus (n=l).Superselective pressure neasurement of venous sinus showed that the pressure difference between the two ends of the narrowed venous sinus was up to 120-580 mmH20 in these 10 patients.In 8 patients (61.5%,8/13) the venous sinus stenosis and/or occlusion might be implicated in the occurrence of ⅡH,in 2 patients (15.4%,2/13) the venous sinus stenosis might be secondary to ⅡH,and in 3 patients (23.1%,3/13) the venous sinus stenosis might bear no relationship to ⅡH.Conclusion Cerebrovascular interventional angiography combined with superselective pressure measurement of venous sinus can clearly identify the relationship between ⅡH and venous sinus
5.Establishment of a scoring system for predicting the positive rate of prostatic biopsy for prostate cancer.
Jian-gang CHEN ; Xin-feng CHEN ; Dong-hua GU ; Ming LU ; Bing ZHENG ; Bing ZHANG ; Bin PAN ; Hua ZHU ; Xiao-dong PAN ; Bin XU ; Lin QIAN
National Journal of Andrology 2015;21(1):53-56
OBJECTIVETo identify the predictors of the positive results of transrectal ultrasound (TRUS)-guided biopsy for prostate cancer.
METHODSWe performed univariate and multivariate logistic regression analyses on the relevant data on 385 male patients that underwent TRUS-guided biopsy for prostate cancer, including such potential predictors as age, body mass index (BMI), symptoms, results of digital rectal examination (DRE), tPSA, fPSA, free/total PSA ratio (f/tPSA), prostate volume (PV), and PSA density (PSAD) for identification of the risk factors related to the positive rate of biopsy. Then we constructed a scoring system as a tool for predicting prostate cancer in repeat biopsies and determined the sensitivity of the system by calculating the false positive rate using the receiver operating characteristic curve.
RESULTSAmong the 385 patients, 139 (36.1%) were diagnosed with prostate cancer. On multivariate analysis, age (P < 0.01), DRE (P < 0.01), tPSA (P < 0.01), fPSA (P < 0.01), f/tPSA (P < 0.01), PV (P < 0.01), and PSAD (P < 0.01) were all significant predictors of prostate cancer. Multivariate logistic regression analysis showed age, tPSA, f/tPSA, PV, and PSAD to be independent predictors, with ORs and 95% CIs of 1.07 (1.05-1.16), 1.05 (1.02-1.15), 0.97 (0.86-0.99), 0.98 (0.87-0.96), and 1.79 (1.48-2.06), respectively. Moreover, patients with the risk score of 3-5 had a significantly higher rate of prostate cancer than those with 0-2 (64% vs 11%, P < 0.001).
CONCLUSIONThe scoring system on the key predictors of prostate cancer can help urologists to identify the men in need of prostatic biopsy.
Aged ; Digital Rectal Examination ; Humans ; Image-Guided Biopsy ; methods ; Logistic Models ; Male ; Middle Aged ; Prostate ; pathology ; Prostate-Specific Antigen ; analysis ; Prostatic Neoplasms ; chemistry ; pathology ; ROC Curve ; Risk Factors ; Ultrasonography, Interventional ; methods
7.Studies on chemical constituents in bark of Larix olgensis var. koreana.
Bao-hua YANG ; Wei-dong ZHANG ; Zheng-bing GU ; Ting-zhao LI ; Chuang ZHANG ; Yun ZHOU
China Journal of Chinese Materia Medica 2005;30(4):270-272
OBJECTIVETo study the chemical constituents in bark of Larix olgensis var. koreana.
METHODThe compounds were isolated with silica gel column chromatography and their structures were elucidated on the basis of spectral analysis (IR, EI-MS, 1H-NMR, 13C-NMR).
RESULTEight compounds were isolated and identified as isopimaric acid (I), beta-sitosterol (II), 24R,5alpha-stigmast-3,6-dione (III), larixol (IV), ferulic acid (V), lariciresinol (VI), secroisolariciresinol (VII) and isolariciresinol (VIII).
CONCLUSIONAll the compounds were isolated from this plant for the first time.
Carboxylic Acids ; chemistry ; isolation & purification ; Furans ; chemistry ; isolation & purification ; Larix ; chemistry ; Lignans ; chemistry ; isolation & purification ; Phenanthrenes ; chemistry ; isolation & purification ; Plant Bark ; chemistry ; Plants, Medicinal ; chemistry ; Sitosterols ; chemistry ; isolation & purification
8.Characteristics of P2X purinoceptors in the membrane of rat trigeminal ganglion neurons.
Yuan-Zheng GU ; Guang-Fu YIN ; Bing-Cai GUAN ; Zhi-Wang LI
Acta Physiologica Sinica 2006;58(2):164-170
The characteristics of purinoceptors in the membrane of rat trigeminal ganglion (TG) neurons were studied by using whole- cell patch clamp technique. The results showed that most of neurons examined (78.9%, 142/180) were responsive to ATP in a concentration-dependent manner; the others (21.1%, 38/180) were ATP insensitive. Of the ATP-sensitive cells, the majority (95.1%, 135/142) responded to ATP with an inward current, a few (2.1%, 3/142) with an outward current, and the rest (2.8%, 4/142) with biphasic current. Small sized cells (<30 mum) responded to ATP with a rapid desensitizing inward current and were highly sensitive to vanilloid; the medium sized cells (30~40 mum) responded to ATP with slow desensitizing inward current and were not sensitive to vanilloid; while the majority of large sized cells (>40 mum) did not respond to ATP and vanilloid. The waveform of ATP-activated inward currents was related to the cell diameter. The I-V curves for both small and medium sized cells manifested obvious inward rectification. Furthermore, we studied the kinetic features of ATP-activated currents and the effects of P2 purinoceptor agonists and antagonists on I(ATP). The findings suggest that ATP receptor-ion channels are expressed differently among different types of rat TG neurons.
Adenosine Triphosphate
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metabolism
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Animals
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Animals, Newborn
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Neurons
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metabolism
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physiology
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Patch-Clamp Techniques
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Rats
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Rats, Sprague-Dawley
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Receptors, Purinergic P2X
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physiology
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Trigeminal Ganglion
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cytology
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metabolism
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physiology
9.Repair of brachial plexus lower trunk injury by transferring brachialis muscle branch of musculocutaneous nerve: anatomic feasibility and clinical trials.
Xian-you ZHENG ; Chun-lin HOU ; Yu-dong GU ; Qi-lin SHI ; Shi-bing GUAN
Chinese Medical Journal 2008;121(2):99-104
BACKGROUNDThere are few effective methods for treating injuries to the lower trunk of brachial plexus, and the curative effect is usually poor. The purpose of this study was to provide anatomic references for transferring the brachialis muscle branch of musculocutaneous nerve (BMBMCN) for selective neurotization of finger flexion in brachial plexus lower trunk injury, and to evaluate its clinical curative effects.
METHODSMicroanatomy and measurement were done on 50 limbs from 25 adult human cadavers to observe the origin, branch, type of the BMBMCN and median nerve, as well as their adjacent structures. Internal topographic features of the fascicular groups of the median nerve at the level of the BMBMCN were observed. In addition, the technique of BMBMCN transfer for selective neurotization of finger flexion of the median nerve was designed and tested in 6 fresh adult human cadavers. Acetylcholinesterase (AchE) staining of the BMBMCN and median nerve was done to observe the features of the nerve fibers. This technique was clinically tried to restore digital flexion in 6 cases of adult brachial plexus lower trunk injury. These cases were followed up for 3, 6, 9 and 12 months postoperatively. Recovery of function, grip strength, nerve electrophysiology and muscle power of the affected limbs were observed and measured.
RESULTSThe brachialis muscle was totally innervated by the musculocutaneous nerve (MCN). Based on the Hunter's line, the level of the origin of the BMBMCN was (13.18 +/- 2.77) cm. AchE histochemical staining indicated that the BMBMCN were totally made up of medullated nerve fibers. At the level of the BMBMCN, the median nerve consistently collected into three fascicular groups as shown by microanatomy in combination with AchE stain. The posterior fascicular group was mainly composed of anterior interosseous nerves and branches to the palmaris longus. The technique was tested in six fresh cadavers successfully, except that stoma split occurred in one case. Five of the six cases recovered digital flexion 12 months after operation, and at the same time grip strength, muscle power, and nerve electrophysiology also recovered markedly.
CONCLUSIONSThe technique of transferring the BMBMCN for selective neurotization of finger flexion is anatomically safe and effective, with satisfactory clinical outcomes.
Acetylcholinesterase ; analysis ; Adult ; Brachial Plexus ; anatomy & histology ; injuries ; Brachial Plexus Neuropathies ; surgery ; Clinical Trials as Topic ; Female ; Humans ; Male ; Middle Aged ; Musculocutaneous Nerve ; transplantation ; Nerve Transfer ; methods ; Retrospective Studies
10.Determination of flavoniod-glycosides in Yixintong pills by HPLC.
Juan SU ; Wei-dong ZHANG ; Yun ZHOU ; Jing ZHOU ; Zheng-bing GU
China Journal of Chinese Materia Medica 2004;29(6):525-527
OBJECTIVETo develop an HPLC method for determining flavoniod-glycosides in Yixintong pills.
METHODAgilent ZORBAX Extend-C18 (4.6 mm x 250 mm, 5 microm) was used with tetrahydrofuran-methanol-acetonitrile-water-acetic acid (30:3:4:120:3) as mobile phase. Detection wavelength was 330 nm.
RESULTGood linearities of rhamnosylvitexin and vetexin-glucoside were obtained within the range of 0.044-1.78 microg (r = 0.9999) and 0.042-0.85 microg (r = 0.9999); the average recoveries were 100.0% and 99.2%; RSD were 0.92% and 1.8%, respectively.
CONCLUSIONThis method can be used for quality control of Yixintong pills.
Apigenin ; analysis ; Chromatography, High Pressure Liquid ; Crataegus ; chemistry ; Drugs, Chinese Herbal ; administration & dosage ; chemistry ; isolation & purification ; Plant Leaves ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control