Objective To observe the anesthetic effects of articaine hydrochloride and epinephrine tartrate injection in oral treatment of elder patients.Methods 66 teeth of 56 elder patients were treated.Pain intensity was scaled by VAS.Pulse and blood pressure were observed before and after drugs administration for 0,5 minutes.ResuIts Pain during injection of articaine hydrochloride and epinephrine tartrate injection and surgery subsequently was rare.The total respouse rate was 95%～100%.No significent toxic effects were found.Pulse and blood pressure remained after injection.Conclusion Articaine hydrochloride and epinephrine tartrate injection was one of local anesthetics with good infiltration,safety and anesehetic effect.It would be more widely used in elder patients oral treatment field in the future.

Objective To evaluate the clinical effects of magnetic attachments in complete overdenture. Methods Casting method was used to fabricate Coping-Keepers for remained roots, and the patients were followed up for 6 months to check the effects of the overdentures retained with Magfit EX attachments. In the experimental group, 21 complete overdentures were made with 33 Magfit EX magnetic attachments for 18 patients. Eleven patients treated with traditional complete overdentures were selected to act as control. The retention force, aspect and comfortableness were subjectively evaluated by the patients of the both groups. Abutment status was observed and compared before and after cementation of the magnetic attachments by measuring periodontal index, including gingival index (GI), sulcus bleeding index (SBI) and bleeding on probing (BOP), etc. Masticatory function of patients in the both groups was measured at 3 time points: immediate, 3 months and 6 months after cementation. Results Patients in experimental group were more satisfied with complete overdentures made up of magnetic attachments than those in control group, especially in retention force and masticatory efficiency and aspect. Magnetic attachments can provide enough retention force for patients and greatly improved the masticatory efficiency and the abutment condition. The masticatory function was to be perfect and stable after 3-6 months. Furthermore, the mobility and periodontal index of most abutments improved after 6 months. Conclusion It is proved that magnetic attachments are effective to improve the function of complete overdentures, worthy to be used widely. It is suggested that the residual root and loosened tooth should be preserved furthest as possible in clinic.

Objective To explore the procedures of breast repair and reconstruction after mastectomy with three methods for optimal appearances of the breast.Methods Three methods were adopted in this study:(1)Two-stage breast reconstruction operation with ampliate latissimus dorsi myocutaneous flap after mastectomy;(2)One stage breast reconstruction operation with transverse rectus adbominis myocutaneous(TRAM)flap after mastectomy;and(3)Two-stage breast reconstruction and nipple repair operation with tissue expander/implant after mastectomy preserving pectoralis major museulus or implants along under pectoralis major musculus.Results All of the flaps survived well and were well-shaped in total 10 patients(10 breasts)with a flap breast reconstruetion,in which the superior ratios were 83.33%.The appearance and volume were not satisfactory in 1 patiant treated with the method of tissue expander/implant,because the volume of tissue inflation was smaller than another one and 100 ml implant was applied.The partial flap necrosis occurred in 1 case with the method of TRAM flap.Conclusions With the development of plastic surgery technique,the appearances and shapes of reconstructed breast achieve the satisfactory effects.After the ampliate latissimus dorsi myocutaneous flap is applied,the appearance and volume of reconstructed breast are similar to the healthy one.The two-stage nipple repair operation is applied with breast reconstruction operation by the tissue expander/implant after mastectomy preserving pectoralis major musculus.According to the different methods of mastectomy to choose suitable procedures of breast reconstruction,the satisfactory appearances and psychotherapy will be achieved.

Objective To detect the differential expression profile of microRNAs between patients with or without gall?bladder stone. Methods Samples from 30 patients with gallbladder stones (GS) and 30 without gallbladder stones (GP) were collected, in which microRNAs expression profiles were examined using high-throughput sequencing instrument Illumi?na HiSeq 2500. MicroRNA sequences were obtained and compared to Genebank and Rfam database for classification. Differ?entially expressed microRNAs were screened, and their target genes were predicted. Significant enrichment analysis of GO and KEGG were performed. Real-time quantitative PCR was performed on selected miRNAs in order to validate their expres?sion. Results Clean tags were obtained from both GS group (n=2 215 832) and GP group (n=1 424 770). A total of 17 mi?croRNAs were differentially expressed between GS and GP groups with statistical significance, among which 9 were up-regu?lated and 8 were down-regulated in GS group compared to those in GP group. GO (Gene ocology) analysis showed that target genes were enriched in ion binding and transport, apolipoprotein binding, calcium channel activity, protein kinase activity, steroid hormone biosynthesis and metabolism. KEGG(Kyoto Encyclopedia of Genes and Genomes)analysis is shown for the target genes enriched in cancer related pathways, including WNT, HIPPO pathways. qRT-PCR validation of some differen?tially expressed miRNAs confirmed the result of high-throughput data analysis. Conclusion The differential expression levels of microRNAs may play an important role in occurrence and development of gallbladder stones.

This study is to establish the gastric hot model of rats. After gastric feeding with ethanol solution for 3 weeks and feeding with extra capsaicin and ethanol solution for another 2 weeks, model group show distinct physical sign of gastric hot syndrome. The pathology of gastrics reveals gastricism of model group, while treatment group (treat with Zuojin Wan) shows mild lesion. Elisa detection of model group show that the solution of interleukin-2 (IL-2) is higher than the blank group. The obvious difference among model group, treatment group and blank group reveals the success of the establishment of gastric hot model.
Animals ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Eating ; Female ; Humans ; Male ; Rats ; Rats, Wistar ; Stomach Diseases ; drug therapy ; pathology ; physiopathology

This study is to establish the gastric cold model of rats. After gastric feeding with cold water for 5 weeks and extra iced water bath in the last 2 weeks, model group show distinct physical sign of gastric cold syndrome. The pathology of gastrics reveals gastricism of model group, while treatment group(treated with Fanzuojin Wan) show mild lesion. Elisa detection of model group show that the solution of interleukin-2 (IL-2) is higher than blank group. The difference with significance among model group, treatment group and blank group reveals the success of the establishment of gastric cold syndrome.
Animals ; Cold Temperature ; Disease Models, Animal ; Female ; Humans ; Male ; Rats ; Rats, Wistar ; Stomach ; chemistry ; metabolism ; pathology ; physiopathology ; Stomach Diseases ; metabolism ; pathology ; physiopathology

Objective To explore the effects of upper limb robot-assisted therapy on motor recovery in acute stroke patients. Methods From August, 2013 to September, 2014, 46 acute stroke patients at their first-ever stroke were enrolled and randomized into experimental group and control group with 23 cases in each group. Both groups received routine therapy. Additional robot-assisted therapy was provided to the experimental group, and additional repetitive movement training was provided to the control group, 30 minutes a day, 5 days a week for 12 weeks. Fugl-Meyer Assessment-Upper Limb (FM-UL), modified Ashworth Scale (MAS) and modified Barthel index (MBI) were used to assess the motor function of the upper limbs and hands, the muscular tension of elbow, and activities of daily living (ADL) before and after treatment. Results After treatment, the scores of FM-UA, MAS and MBI improved in both groups (t>3.856, Z>1.889, P<0.05), and the scores of FM-UA and MAS were better in the experiment group than in the control group (t=-2.386, Z=-2.625, P<0.05), however, there was no significant difference in the score of MBI between two groups (t=-1.326, P=0.098). Conclusion Upper limb robot-assisted therapy can facilitate the recovery of the motor function of upper limbs in acute stroke patient.

Objective To investigate the clinic value of enhanced CT-guided percutaneously transthoracic lung biopsy. Methods A total of 423 patients who underwent CT-guided percutaneously transthoracic lung biopsy were retrospectively analyzed. The percentage of specimen are satisfactory,the rate of sample with pathological findings,the number of cases with complications,the average goes of unsuccessful biopsy and the times of changing puncture point were compared between the enhanced CT group and the plain CT group. Results Compare parameters in patients whose procedure were guided with the enhanced CT group and with plain CT group, the average goes of unsuccessful biopsy, the times of changing puncture point in patients, the percentage of specimen that were satisfactory, the rate of sample with positive pathological findings and the rate that complicated with pneumothorax or hemorrhage show statistical significance.(3.08±1.05 vs 3.75±1.34, 8.52%vs 18.54%,90.13%vs 73.60%, 76.62%vs 63.36%, 15.51%vs 27.53%, 7.76%vs 14.04%, P<0.05). Conclusion In patient whose biopsys were guided by enhanced CT, the percentage of samples that are satisfactory is higher, the incidence of complication is fewer and the sample with pathological finding is higher compared to those in patients whose biopsys were guided by plain CT. Therefore, enhanced CT-guided percutaneous transthoracic biopsy is more valuable than plane CT.

BACKGROUND:Bone marrow mesenchymal stem cells can survive under the lethal dose of radiation in response to hematopoietic stem cells, and stil maintain the typical characteristics of stem cells to promote hematopoietic recovery after radiation. However, autophagy is an important mechanism for cellular adaptation under stress, which may be involved in radiation tolerance of bone marrow mesenchymal stem cells. OBJECTIVE:To investigate the role of autophagy in human bone marrow mesenchymal stem cells in response to irradiation. METHODS:Passage 3 bone marrow mesenchymal stem cells cultured in vitro at logarithmic phase were col ected and randomized into control, 3-mehyladenine, rapamycin, irradiation, irradiation+3-mehyladenine and irradiation+rapamycin groups. The autophagy reactions of bone marrow mesenchymal stem cells were regulated by 5 mmol/L 3-mehyladenine and 200 nmol/L rapamycin for 12 hours in the 3-mehyladenine and rapamycin groups, respectively. Two-hour 6 Gy X-ray irradiation was performed in the irradiation group and two complication groups undergoing 12-hour corresponding drug intervention. RESULTS AND CONCLUSION:The proportions of cells with autophagic vacuoles and apoptotic cells were higher in the irradiation group than the control group, moreover, autophagic+apoptotic cells were increased in the irradiation group. 3-mehyladenine intervention could decrease the proportion of cells with autophagic vacuoles,and increase the number of apoptotic cells. But there was no difference in the proportion of autophagic+apoptotic cells between the 3-mehyladenine and irradiation groups. After rapamycin intervention, the proportion of autophagic cells was higher than that in the irradiation group, but no difference in the proportion of apoptotic cells between the two grups, as wel as there were no autophagic+apoptotic cells. The expression of microtubule-associated protein 1 light chain 3-II was ranked as fol ows:the control and 3-mehyladenine groups

This study was aimed to observe the effect of electro-acupuncture (EA) treatment on Zhong-Xiao (ZX, T2MD) with obesity. A total of 203 patients with ZX accompanied with obesity accepted EA treatment. Before and after 3-month treatment, observations were made on patients' fasting blood glucose (FBS), fasting insulin (FINS), in-sulin sensitivity index (ISI), insulin resistance index (Homa-IR), insulin secretion index (Homa-β), degree of obesity (A), body mass (Kg), body mass index (BMI) and body fat percentage (F%) changes. The results showed that com-pared with pretreatment, the posttreatment FINS, Homa-IR, FBS had a significant decrease among 203 patients (P<0.01); the ISI and Homa-β had a significant increase (P < 0.01). There was a significant statistical difference on islet β-cell function before and after treatment. The A, body mass, BMI, and F% were significantly decreased (P<0.01). It was concluded that EA can significantly correct ZX with obesity patients' glucose consumption, protect the islet β-cell function, and reduce the body mass of patients.