Acupuncture Points ; Acupuncture Therapy ; Adult ; Female ; Humans ; Male ; Middle Aged ; Shoulder Pain ; therapy ; Young Adult

AIM: To investigate the effects of c-fos antisense oligoneuleotide and p21 genetic transfection on the intimal proliferation of venous autografts. METHODS: The external jugule veins were autografted into common carotid arteries in the same side in 20 New Zealand rabbits, which were divided evenly into experimental and control group randomly. The transplanted veins of experimental group were immersed in the adenovirus - mediated p21 gene solution for 15 minutes just before anastomosis and coated with c-fos antisense oligoneucleotide glue gel just after anastomosis, while the control was only treated with empty vector. The transplanted vascular sample were taken at 2 weeks after operation. The intimal thickness (IT), degree of restenosis (DR), expression of proliferating cell nuclear antigen (PCNA), quantity of VSMC were determined by immunohischemistry. RESULTS: The IT , DR and expression of PCNA, VSMC were decreased, compared to control group. CONCLUSION: Transfection of c-fos antisense oligoneuleotide and p21 gene inhibits the intimal proliferation of venous antografs. [

PNAS-4 is a novel pro-apoptosis gene identified latetly. In recent years, there has been a large number of research reports on the basic studies about PNAS-4 in cancer gene therapy and gene therapy of PNAS-4 alone or combined with chemotherapy or radiotherapy manifested a good application prospect, but its molecular mechanisms to promote apoptosis is not clear yet. In this paper, recent research about PNAS-4 in cancer gene therapy is briefly reviewed, and recent hypotheses on its molecular mechanisms to promote apoptosis are especially elucidated. Based on its newly identified characteristics of structural domain, we made a point that PNAS-4 might regulate functions of some target protein related to apoptosis by deSumoylation as a new deSumoylating isopeptidase, and consequently promote apoptosis.
Apoptosis ; Apoptosis Regulatory Proteins ; genetics ; Genetic Therapy ; Humans ; Neoplasms ; therapy ; Sumoylation

Endocrine Disruptors ; Environmental Exposure ; adverse effects ; Environmental Pollutants ; Humans ; Obesity ; epidemiology

During the last fifty-year medical treatments have made great advances in the treatment of heart failure(HF).The current applicatin of treatment improves the life quality of patients with HF,and reduces their clinical envents and mortality.But the patients with refractory heart failure(RHF) have poor prognosis.This article reviews and analyzes the advances in the therapies for patients with RHF.

Artemisinin is a sesquiterpene lactone compounds,their derivatives include mainly dihydroartemisinin,artesunate,artemether and Artemisia ether. These drugs are now mainly used for clinical treatment of malaria. With the pharmacological action of artemisinin and its derivatives have been further studied,except antimalarial effects,researchers have found their anti-inflammatory,anti-sepsis,antitumor,radiosensitization,antimicrobial sensitivity,anti-fibrosis effects in recent years. Here,we collect and review the most newly pharmacological effects of artemisinin and its derivatives all over the world in past years.

AIM: To analyze single nucleotide polymorphisms (SNP) of primary open angle glaucoma- and metabolic syndrome-related genes in primary open angle glaucoma (POAG), in order to elucidate the roles of metabolic syndrome as a risk factor in POAG progress.METHODS: SNP genotypes and alleles of interleukin-6 (IL- 6), IL- 6 receptor (IL- 6R), dopamine D2 receptor (DRD2), beta-fibrinogen (FGB), peroxisome proliferator-activated receptor-γ2 (PPARG), transforming growth factor-β1 (TGF-β1), E-selectin (E-Sel), apolipoprotein A-5 (APOA5), C-reactive protein (CRP), ectonueleotide pyrophosphatase/ phosphodiesterase 1 (ENPP1), hepatic lipase (LIPC), adiponectin (ADIPOQ), paraoxonase 1 (PON1) and serine protease inhibitor E (SERPINE1) genes in POAG (n= 37) and normal control (n=100) groups were measured with ABI Prism 7900HT Fluorescence Quantitative PCR and TaqMan SNP Genotyping fluorescence probe kit.RESULTS: Genotypes and allele frequencies of IL- 6R, IL- 6, FGB, CRP, ENPP1, LIPC, ADIPOQ, PON1, and SERPINE1 in total POAG group were significantly different compared to the control group. CONCLUSION: Metabolic syndrome as a risk factor for POAG may be associated with genotypes and allele frequencies of the related genes.The corresponding gene expression and function can affect POAG progress, including roles of SERPINE1 in extracellular matrix, ENPP1 in insulin inhibition, IL- 6 in endogenous neuroprotection, IL- 6, IL- 6R and E-Sel in autoimmune response, LIPC and FGB in blood hyperviscosity syndrome, ADIPOQ in NOS/NO production, PON1 in vascular endothelial protection.

The studies of nutritional status and biochemical indices of obese children were taken in three elementary schools with different socioeconomic levels. Subjects including obese and overweight children were matched with normal weight children by the ratio of 1:1. The caloric intake of the obese children was obviously higher than that of the normal children. There were significant differences between the obese and the normal children in concentrations of Hb and serum total protein (p

Objective To investigate the effect of chuan kezhi injection atomizing inhalation in the prevention of respiratory infection after general anesthesia postoperative.MethodsA total of 74 cases with fractures and brain surgery postoperative with intubation anesthesia from Jiaxing First Hospital were collected and randomly divided into two groups with 37 cases in each group.Patients in the control group were treated by routine atomizing inhalation, patients in the experiment group were treated by chuan kezhi injection atomizing inhalation, before and after treatment empty stomach sampled venous blood 5 mL, determined T cell subgroup, observed cough and expectoration eased and throat discomfort alleviated situations of the patients after operation 1 to 4 days, and compared the clinical efficacy.ResultsAfter treatment the effective rate of the experiment group 94.59% was higher than the control group 81.08%, with no statistical significance;the cough and expectoration symptoms eased of the experiment group were significant better than the control group after treatment 1, 2, 3d (P<0.05), the throat discomfort symptoms eased of the experiment group was significant better than the control group at treatment 3 d (P<0.05), after treatment the CD3+, CD4+/CD8+ and NK cell levels of the experiment group were higher than the control group(P<0.05).ConclusionChuan kezhi injection atomizing inhalation can effectively ease throat symptoms after general anesthesia, improve immune status, the effect is remarkable.

The eukaryotic expression vector pREP-8-IL-2 or and pREP-8-IFN-? was injected i.p. into mice by calcium phosphate coprecipitation method and IL-2 gene or IFN-? gene was successfully transfected into peritoneal M?s whose expression products could enhance the cytotoxicity of M?s, which secrete some TNF, IL-1 and NO, activate the nonspecific immunity and inhibit the growth of tumor effectively. In particular, IL-2 gene in combination with IFN-? gene transfection were successfully transfected into peritoneal M?s whose high expression products not only significantly enhance the M?s cytotoxicity and made it secrete high level TNF, IL-1 and NO, but also activated the CTLs of spleen and initiated specific immunity and nonspecific immunity . This would produce synergic antitumor effects. The results showed that IL-2 and IFN-? gene transfection produced more antitumor effects than IL-2 or IFN-? gene transfection alone.