1.Atypical HUS caused by anti-complement factor H antibody: a hematologist's perspective.
Meet KUMAR ; Prakas MANDAL ; Rajib DE ; Pinaki MUKHERJEE ; Tuphan Kanti DOLAI ; Maitryee BHATTYACHARYYA
Blood Research 2015;50(1):63-65
No abstract available.
Complement Factor H*
2.Diagnostic value of renal function parameter detection of early renal damage in multiple myeloma.
Journal of Experimental Hematology 2013;21(1):241-244
Renal damage is one of the most common complications and cause of death in patients with multiple myeloma (MM). The studies have pointed out that early renal impairment is risk factor for progress of this disease, timely diagnosis and prompt intervention therapy are very important to improve the prognosis and survival of MM patients. Therefore, the diagnosis of early renal damage is crucial for clinical treatment. The progress on detection of early renal damage parameters and their value are reviewed in this article.
Alpha-Globulins
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urine
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Humans
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Kidney
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physiopathology
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Multiple Myeloma
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diagnosis
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physiopathology
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Proteinuria
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Retinol-Binding Proteins
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urine
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beta 2-Microglobulin
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urine
3.Effects of birth asphyxia or intrauterine distress on renal functions in newborns in the first week of life.
Yong CAI ; Zong-De XIE ; Ping-Yang CHEN ; Yi-Ling DING
Chinese Journal of Contemporary Pediatrics 2006;8(3):184-186
OBJECTIVETo investigate the renal function in newborns with birth asphyxia or intrauterine distress in the first week of life.
METHODSSixty full-term newborns born between June 2002 and February 2003 were assigned into three groups: Control group (healthy newborns), Intrauterine distress group (Apgar score > 7), and Birth asphyxia group without intrauterine distress (12 mild asphyxia and 8 severe asphyxia) (n=20 each). Urinary levels of alpha1-microglobulin (alpha1-MG), beta2-microglobulin (beta2-MG) and albumin (Alb) were detected by radioimmunoassay at 0-2, 3-4 and 6-7 days after birth.
RESULTSThe urinary levels of alpha1-MG, beta2-MG and Alb in the Asphyxia group were significantly higher than those in the Control group at all time points (P < 0.05), peaking at 3-4 days after birth. Statistically significant differences were found between the severely and mildly asphyxiated newborns for the urinary levels of alpha1-MG, beta2-MG and Alb at all time points (P < 0.05). There were no significant differences in the urinary levels of alpha1-MG, beta2-MG and Alb between the Intrauterine distress and the Control groups at each time point.
CONCLUSIONSBirth asphyxia may lead to renal glomerular and tubular impairments and it is speculated that the most serious impairment occurs at the 3rd and 4th days of life. The severity of renal impairments is associated with the degree of asphyxia. The renal function of the newborn appears to be normal following intrauterine distress.
Albuminuria ; urine ; Alpha-Globulins ; urine ; Asphyxia Neonatorum ; physiopathology ; Fetal Distress ; physiopathology ; Humans ; Infant, Newborn ; Kidney ; physiopathology ; beta 2-Microglobulin ; urine
4.Paper electrophoretic pattern of serum protein fractions in various parasitic diseases.
Won Young CHOI ; Ok Ran LEE ; Won Koo LEE
The Korean Journal of Parasitology 1974;12(2):157-164
Electrophoretic patterns of human blood serum protein were studied in parasitic diseases including 19 cases of clonlrchiasis, 29 cases of clonorchiasis and other helminthiasis and 22 cases of healthy controls in Kim-hae area. The same study was made on 32 cases of ascariasis, 56 cases of trichocephaliasis, 80 cases of ascariasis and trichocephaliasis, 9 cases of amebiasis and 44 cases of healthy controls in the Seoul Juvenile Center. The total serum protein was performed by Folin method and the serum protein fraction test by Durrum's paper electrophoretic method. The results were as follows: In the case of clonolrchiasis, the total serum protein, albumin fraction and A/G ratio decreased and alpha (1) globulin fraction increased twice as much as in the case of healthy controls. In the case of clonorchiasis and other helminthiasis, alpha(1) globulin fractions increased. In the case of ascariasis, albumin fractions increased but alpha(1) globulin fractions decreased. In the case of ascariasis and trichocephaliasis, alpha(2) and beta globulin fractions decreased. In the case of amebiasis, alpha(1) globulin decresed.
parasitology-helminth-trematoda
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electrophoresis-immunology
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serum
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alpha-1 globulin
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alpha-2 globulin
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beta globulin
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protein
5.The experimental studies on Capillaria hepatica.
The Korean Journal of Parasitology 1964;2(1):63-77
Capillaria hepatica is an extremely common parasite of rats. Several human cases have also been reported from various parts of the world and recently these aroused the clinical interests. The present study was undertaken to investigate the biological observations of C. hepatica and the changes occurring in blood picture and serum protein in the experimentally infected hosts. The source of C. hepatica obtained from the deposit of non-embryonated eggs encapsulated in the liver of house rats(Rattus norvegicus) in Seoul. The eggs isolated from the infected liver tissues by the freshly prepared artificial gastric juice at 37 C and embryonated in the incubator 27 to 30 C for four to five weeks. For the observation of migratory pathway to the liver, ten mice were infected orally with 1,000 embryonated eggs of C. hepatica, and another ten mice were infected intraperitoneally. No larvae were found in the washings of peritoneal cavity after oral infection, but after the third day of infection, the larvae were isolated from liver tissues. These indicated that the majority of larvae are transported to the liver by the hepatic portal system. On the other hand, 1,000 embryonated eggs of C. hepatica were inoculated into the peritoneal cavity of mice by mantoux syringe containing antibiotics. One third of inoculated eggs hatched out in the peritoneum during two days after inoculation, hatched in the peritoneal cavity invade directly to the surface of liver. Twenty white rats were infected orally with 1,000 to 2,000 embryonated eggs for the study of the development of C. hepatica in the liver and histopathological changes of the infected liver in the course of infection. C. hepatica in the liver of white rats developed rather slowly at the first tenth day after infection, but at the 13th day developed rapidly in its size. The worms were sexually differentiated at the l7th day after infection. At the 20th fully formed eggs appeared in the white or yellowish lesions on the surface of rat liver and they are also found in uterine tubule of the female worm. After the 33rd day, male worm disappeared and only female worms packed with eggs were detected in the liver tissues. However the long hair-like tightly coiled worms were also usually found in the hepatic cysts, and the degenerated or dead worms were observed in the small cysts on the surface of the liver at the 59 th day after infection. Microscopical examination on the first week after infection revealed inflammatory reactions with the dilatation of central vein, Kupffer cell mobilization, focal necrosis and perivascular infiltration. After two weeks of infection granulomatous inflammation were observed around or adjacent to the worms in the lobules. The worms are surrounded by macrophages, multinucleated giant cells, a dense infiltration of lymphocytes, monocytes, neutrophils and, especially, eosinophils. After the third and fourth week, the microscopical findings of infected rat livers have shown proliferation of connective tissues and regeneration of liver cells. During the fifth to sixth week after infection, rat liver showed marked proliferation of fibrous connective tissues encapsulated the worms and massive deposition of the eggs. At the later time the liver reveals many pseudolobules which are caused by postnecrotic cirrhosis. These are irregularly subdivided into lobule by a fibrous septum. The worms were fragmented by the phagocytes and encapsulated by connective tissues. And then finally they appeared to be replaced by the calcium-like material. The liver shows typical cirrhosis after the eighth week after infection. In order to investigate the changes of blood picture and serum protein components of rabbits infected with C. hepatica, twenty rabbits were divided into four groups by the doses of eggs. Group A was given doses of 1,000 embryonated eggs, group B 5,000 eggs, group C 10,000 eggs and group D 30,000 eggs. The pictures of blood especially leukocyte and eosinophil counts and of serum protein were checked every week for ten weeks in the course of infections. The marked elevation of the leukcocyte, eosinophil counts and percentage of eosinophils was observed at the sixth to the seventh week in the course of infection in all groups of rabbits. At the tenth week after infection a decrease was shown in their counts. However in the heavily infected groups (Group C and D) these values persisted relatively in high levels even thereafter. In the white rats given doses of 1,000 to 2,000 eggs, eosinophil counts increased to the peak at the fourth week and decreased at the seventh week after infection. The changes in serum protein components of infected rabbits were investigated by paper electrophoresis. Blood collections were done by the cardiac puncture in the early morning. Serum total protein was determined by Biurets method, serum protein fractionating and A/G ratio by paper electrophoresis using Whatman No.l filter paper and barbital buffer (pH 8.6, ionic strength 0.06). Total protein increased at the sixth and seventh week after infection and the albumin and A/G ratio had decreased significantly in the heavily infected groups at the fifth and sixth week. The alpha-globulin and beta-globulin were not significant in the lightly infected groups(Group A and B), but they decreased after seventh week in the heavily infected groups. The gamma-globulin and gamma/A ratio of the heavily infected groups were significantly increased at fifth to seventh week. Statistically the calculation of entropy was applied to the data obtained in all groups. In the lightly infected groups, the entropy was included almost in the normal ranges, however in the heavily infected groups it was excluded from the normal range during the first to eighth week after infection.
parasitology
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helminth
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nematoda
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pathology
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biochemistry
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hematology
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rabbit
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rat
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alpha-globulin
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beta-globulin
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gamma-globulin
7.Effects of tank operation on renal function of crews.
Qiang MA ; Hong-Fei WANG ; Chang-Jiang XING ; Hua-Chao MA ; Mei-Liang GONG ; Lei SUN ; Hong-Ling LIANG
Chinese Journal of Applied Physiology 2014;30(5):475-477
OBJECTIVETo explore the effects of harmful factors in tank cabins on renal function of tank crews.
METHODSOne hundred and fifty two tank crews as the observation group and 37 soldiers without tank environment exposure as control group were selected in the study. α1-microglobulin(α1-MG), β2-microglobulin(β2-MG), IgG, N-acetyl-β-glucosidase (NAG) and urinary albumin excretion rate (UAER) in morning and 24 h urine were measured.
RESULTSCompared to the control group, the levels of α1-MG, β2-MG, NAG, UAER in observation group were increased significantly (P < 0.05). β2-MG, NAG, UAER of Soldiers with more than 50 motorized hours in observation group were significantly higher than those of control group (P < 0.05). β2-MG, NAG and UAER of soldiers divorced from tank occupation more than 3 years decreased to the normal levels. β2-MG of soldiers divorced from tank occupation more than 10 years was significantly higher than that of 6-10 years group.
CONCLUSIONTank occupational exposure influences the renal function of tank crews but not to a degree of clinical kidney disease. The renal function of crews divorced from tank occupation may recover but dysfunction of renal tubular reabsorption still exists.
Acetylglucosaminidase ; metabolism ; Albuminuria ; Alpha-Globulins ; metabolism ; Environmental Exposure ; adverse effects ; Humans ; Kidney ; physiology ; Kidney Function Tests ; Military Personnel ; beta 2-Microglobulin ; metabolism
8.Angiostatin Works as Immune Modulatory Molecules via Inhibition of Neutrophil Activation and Migration.
Journal of Bacteriology and Virology 2014;44(1):115-119
Angiostatin is derived from enzymatic degradation of plasminogen and it has endogenous anti-angiogenic properties. Although tumor cells, macrophages, platelets, and neutrophils generate high amount of angiostatin, its expression is increased in inflammatory conditions. Moreover, angiostatin binds to integrin alpha(v)beta(3), ATP synthase, and angiomotin, which expressed on neutrophils. Activated neutrophils are essential to innate immune response, but also cause tissue damage through production of reactive oxygen species (ROS) and increase lifespan. In this article, it suggests several mechanism of angiostatin as immune regulator for neutrophils in inflammatory conditions; complex with integrin alpha(v)beta(3) and F(1)F(0) ATP synthase on lipid raft, attenuate polarization, and ROS production. These data provide possible exploit of double-edged role of neutrophils in acute inflammatory pathologies to preserve beneficial effect and minimize tissue damage.
Adenosine Triphosphate
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Angiostatins*
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Apoptosis
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Immunity, Innate
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Integrin alphaVbeta3
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Macrophages
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Neutrophil Activation*
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Neutrophils*
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Pathology
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Plasminogen
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Reactive Oxygen Species
9.Urinary ?-microglobulin excretion for detecting tubular dysfunction in adult patients with primary nephrotic syndrome
Journal of Vietnamese Medicine 2004;302(9):55-59
The research studied urinary beta2-microglobulin excretion to detect tubular dysfunction in 31 patients with primary nephrotic syndrome. The result showed the increased excretion of beta2-microglobulin was observed in 11/31 cases. There were no sighgicant differences of the urinary creatinine excreation and between group of patients with increased urinary beta2-microglobulin excretion and the group without it. This indicated having combine tubular dysfunction in patients with primary nephrotic syndrome. The finding might suggest the use of urinary beta2-microglobulin for detecting the associated tubular disfuntion in such patients
Diagnosis
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Creatinine
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Beta-Globulins